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Exploring the Transcriptome Landscape of Pomegranate fruit Peel for Natural Product Biosynthetic Gene and SSR Marker Discovery

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Journal of Integrative Plant Biology 2011年第10期53卷 800-813页

作者： Nadia Nicole Ono Monica Therese Britton Joseph Nathaniel fass charles Meyer Nicolet Dawei Lin Li Tian Department of Plant Sciences University of California Davis CA 95616 USA Genome Center University of California Davis CA 95616 USA

Pomegranate fruit peel is rich in bioactive plant natural products, such as hydrolyzable tannins and anthocyanins. Despite their documented roles in human nutrition and fruit quality, genes involved in natural product biosynthesis have not been cloned from pomegranate and very little sequence information is available on pomegranate in the public domain. Shotgun transcriptome sequencing of pomegranate fruit peel cDNA was performed using RNA-Seq on the Illumina Genome Analyzer platform. Over 100 million raw sequence reads were obtained and assembled into 9,839 transcriptome assemblies （TAs）（〉200 bp）. Candidate genes for hydrolyzable tannin, anthocyanin, flavonoid, terpenoid and fatty acid biosynthesis and/or regulation were identified. Three lipid transfer proteins were obtained that may contribute to the previously reported IgE reactivity of pomegranate fruit extracts. In addition, 115 SSR markers were identified from the pomegranate fruit peel transcriptome and primers were designed for 77 SSR markers. The pomegranate fruit peel transcriptome set provides a valuable platform for natural product biosynthetic gene and SSR marker discovery in pomegranate. This work also demonstrates that next-generation transcriptome sequencing is an economical and effective approach for investigating natural product biosynthesis, identifying genes controlling important agronomic traits, and discovering molecular markers in non-model specialty crop species.

关键词： hydrolyzable tannin naturat product pomegranate SSR marker transcriptome.

Synthesis, characterization, and stability of iron (III) complex ions possessing phenanthroline-based ligands

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Open Journal of Inorganic Chemistry 2013年第1期3卷 7-13页

作者： Shawnt Tosonian charles J. Ruiz Andrew Rios Elma frias Jack f. Eichler Department of Chemistry University of California Riverside Riverside USA

It has previously been demonstrated that phenanthroline-based ligands used to make gold metallotherapuetics have the ability to exhibit cytotoxicity when not coordinated to the metal center. In an effort to help assess the mechanism by which these ligands may cause tumor cell death, iron binding and removal experiments have been considered. The close linkage between cell proliferation and intracellular iron concentrations suggest that iron deprivation strategies may be a mechanism involved in inhibiting tumor cell growth. With the creation of iron (III) phen complexes, the iron binding abilities of three polypyridal ligands [1,10-phenanthroline (phen), 2,9-dimethyl-1, 10-phenanthroline (methylphen), and 2,9-di-sec-butyl-1, 10-phenanthroline (sec-butylphen)] can be tested via a competition reaction with a known iron chelator. Therefore, iron (III) complexes possessing all three ligands were synthesized. Initial mass spectrometric and infrared absorption data indicate that iron (III) tetrachloride complex ions with protonated phen ligands (RphenH+) were formed: [phenH][feCl4], [methylphenH][feCl4], [sec-butylphenH][feCl4]. UV-vis spectroscopy was used to monitor the stability of the complex ions, and it was found that the sec-butylpheniron complex was more stable than the phen and methylphen analogues. This was based on the observation that free ligand was observed immediately upon the addition of EDTA to the [phenH][feCl4] and [methylphenH] [feCl4] complex ions.

关键词： Polypyridyl Ligands Phenanthroline Iron (III) Complex Ions

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CALMODULIN MEDIATES THE CA2+ DEPENDENT REGULATION Of LENS GAP JUNCTIONS

丛书名：计算机技术译林精选系列

2000年

作者：（美）charles f.Goldfarb （美）Paul Prescod 潇湘工作室译

本书全面而详尽地介绍了XML。其主要内容有：XML的定义和作用，它与HTML的区别以及各种概念，XML开发的经验等。

ISBN: (纸本)7115086575

本书全面而详尽地介绍了XML。其主要内容有：XML的定义和作用，它与HTML的区别以及各种概念，XML开发的经验等。

关键词：可扩充语言计算机网络

来源：畅想之星图书光盘版（南... 评论

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CALMODULIN MEDIATES THE CA2+ DEPENDENT REGULATION OF LENS GA...

The boys are back in town: Rethinking the function of ribosomal DNA repeats in the genomic era

第十八届国际眼科研究大会

作者： charles f..Louis Monica.Lurtz Jenny.Yang University of California Riverside

To determine the mechanism by which intracellular Caconcentration([Ca])regulates cell-to-cell communication mediated by Cx43,the major connexin in lens epithelial cell gap junctions cell-to-cell communication was dete...

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Molecular Plant 2023年第3期16卷 514-516页

作者： francesca B.Lopez Peter C.McKeown Antoine fort Galina Brychkova charles Spillane Genetics and Biotechnology Laboratory Plant&AgriBiosciences Research Centre(PABC)Ryan InstituteUniversity of GalwayH91 REW4 GalwayIreland Present address:Department of Plant Biology and Genome Center University of CaliforniaDavisUC Davis Genome CenterDavisCA 95616USA

Next-generation sequencing technological advances,affording high-throughput,scalable,and fast assemblies,have enabled genome editing techniques that allow us to remove,insert,and modify genes for rapid and precise fun... 详细信息

关键词： remove precise function

The Crystallization Behavior Regulating Nature of Hydrogen Bonds Interaction on Polyamide 6,6 by Poly(vinyl pyrrolidone)

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Chinese Journal of Polymer Science 2023年第3期41卷 394-404,I0008页

作者： Yang Liu Li Peng Jie-Long Lin Yong Zhou Du-Jin Wang charles C.Han Xian-Bo Huang Xia Dong National-certified Enterprise Technology Center Kingfa Science and Technology Co.LTD.Guangzhou 510663China CAS Key Laboratory of Engineering Plastics Beijing National Laboratory for Molecular SciencesInstitute of ChemistryChinese Academy of SciencesBejing 100190China University of Chinese Academy of Sciences Beijing 100049China

The crystallization behavior of polyamide 6,6(PA66) under complex flow field is of great importance for its final mechanical ***(vinyl pyrrolidone)(PVP) was applied as crystallization modifying agency to improve processability of *** regulation nature on hydrogen bonds(H-bonds) interaction of PVP was studied upon cooling *** revealed by in situ fTIR,the sample with 5 wt% PVP displays decreased enthalpy change(ΔH) for both the generation of H-bonds and the transition of H-bonds from “disordered” to “ordered” state,which illustrates the molecular mechanism for the regulation of crystallzation ***,2D-WAXD is applied to reveal the evolution of microstructure for sample under external injection *** orientation factor(f_(H)) decreases obviously with the addition of PVP,because the relaxation of chain segments is promoted as well the crystallization is *** above crystallization and microstructure changes during the processing provokes the improvement of processability without the apparent sacrifice of mechanical properties.

关键词： Polyamide 66 Poly(vinyl pyrrolidone) Hydrogen bonds interaction

Significance of age and sex in botulinum neurotoxin dosing for adductor spasmodic dysphonia

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World Journal of Otorhinolaryngology-Head and Neck Surgery 2023年第2期9卷 168-173页

作者： Richard Heyes charles H.Adler Nan Zhang David G.Lott Stephen f.Bansberg Department of Otorhinolaryngology-Head and Neck Surgery Mayo Clinic ArizonaPhoenixArizonaUSA Department of Neurology Mayo Clinic ArizonaPhoenixArizonaUSA Department of Biostatistics and Bioinformatics Mayo Clinic ArizonaPhoenixArizonaUSA

Objectives: This study aims to analyze the impact of age and sex on botulinum neurotoxin (BoNT-A) dosing and outcomes in adductor spasmodic dysphonia (AdSD).Methods: A database review of all spasmodic dysphonia patients treated with BoNT from 1989 to 2018 at the Mayo Clinic in Arizona was performed. Only patients who had received ≥4 injections of BoNT-A for AdSD were included. Patients were divided into two cohorts to analyze age, with an age of first treatment cutoff of 60 years. Patients were divided into male and female cohorts to analyze ***: The final analysis included 398 patients. The mean dose of BoNT-A per treatment was significantly higher in the younger cohort (4.4 vs. 3.9 units,p = 0.048). The mean maximal benefit was similar (72% vs. 70%,p = 0.48);however, the mean length of benefit was significantly shorter in younger patients (3.0 vs. 3.6 months,p ficantly higher in the female cohort (4.2 vs. 3.6 units,p = 0.02). The mean maximal benefit was similar (69% vs. 75%,p = 0.58), as was the mean length of benefit (3.2 vs. 3.5 months,p = 0.11).Conclusions: This study suggests that age and sex influence BoNT-A dosing and outcomes in AdSD.

关键词： adductor age gender outcomes sex spasmodic dysphonia

Thick film Circuit Materials

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功能材料 1992年第5期 265-269页

作者： charles C.Y.Kuo CTS Corporation 7 Swanson Manor Elkhart IN 46516 USA 本刊通讯编委 7 Swanson Manor Elkhart IN 46516 USA

THICK fILM CIRCUITS most commonly involve the screen printing process of forming a functional composition through a patterned mesh screen onto *** ceramics. The printed film is dried and fired, typically in the range of 500 to 900 ℃ (930 to 1650°f) to sinter the film and adhere it ro the substrate with the desired characteristics for circuit applications. The functional compositions in general include conductors, resistors, and dielectrics. The dielectrics may be used as overglazes, crossovers, capacitors, or multilayers. The term thick film originated from the term thin film. Thin films are deposited by vacuum technology, either by evaporation or sputtering. In the early 1960s, the Bell Tele-

关键词： high

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瓣膜性心脏病

江西医药 1986年第2期 172-188页

作者： charles f.Rackley

瓣膜性心脏病的临床症象来自瓣膜的狭窄和(或)关闭不全,这些机械性病变使邻近腔室承受压力或容积的超负荷。瓣膜性心脏病中最常受累的心腔是左室,因代偿压力或容积长期负荷过重而扩张、肥厚。心肌的需氧量即与心肌的机械作功和肥厚有关... 详细信息

瓣膜性心脏病的临床症象来自瓣膜的狭窄和(或)关闭不全,这些机械性病变使邻近腔室承受压力或容积的超负荷。瓣膜性心脏病中最常受累的心腔是左室,因代偿压力或容积长期负荷过重而扩张、肥厚。心肌的需氧量即与心肌的机械作功和肥厚有关。瓣膜性心脏病晚期,心肌失代偿,心排出量减低,尽管心肌对氧的需要量仍高,但冠状动脉灌注可能已影响到氧的输送。

关键词：心脏病膜性心腔心脏疾病心脏血管疾病瓣膜心导管检查