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Science China Earth Sciences 2007年第6期50卷 927-935页

作者： ZHAO ZhiZhong QIAO YanSong WANG Yan FU JianLi WANG shuBing LI chaozhu YAO HaiTao JIANG FuChu Institute of Geomechanics Chinese Academy of Geological Sciences Beijing 100081 China

Two red earth sections from the Chengdu Plain in Sichuan Province are dated using geomagnetic methods. The Brunhes/Matuyama (B/M) reversal boundary is recognized within the upper parts of the two sections,and the Jaramillo normal subzone occurs in the middle-lower parts of the vermiculated red soil (VRS),which indicate that the parent material for the VRS deposited in the middle Quaternary. Sedimentologic and geochemical studies suggest that the Chengdu Clay and part of the parent material for the VRS are of aeolian origin. Pollen analysis indicates that the Chengdu Plain was mainly covered by needle-leaved forest with a cool to cool temperate humid climate when the parent material for the VRS deposited. The relatively cool to cool temperate climatic condition in the Chengdu Plain in the middle Quaternary,which may be caused by the uplift of the Tibetan Plateau,was a response to the geo-environmental changes of global significance.

关键词： Chengdu Plain vermiculated red soil magnetostratigraphy origin paleoenvironment

“互联网+护理服务”上门护士岗位胜任力指标体系的系统评价和Meta整合

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中西医结合护理（中英文） 2024年第4期10卷 170-173页

作者：黄南杏车运秋刘佳佳何朝珠南昌大学江西南昌330000

目的系统评价国内外上门护士岗位胜任力评价指标,为中国“互联网+护理服务”上门护士的培养和队伍建设提供参考。方法计算机检索PubMed、Web of Science、Cochrane Library、CINAHL、Embase、CNKI、万方、VIP和SinoMed中关于上门护士岗... 详细信息

目的系统评价国内外上门护士岗位胜任力评价指标,为中国“互联网+护理服务”上门护士的培养和队伍建设提供参考。方法计算机检索PubMed、Web of Science、Cochrane Library、CINAHL、Embase、CNKI、万方、VIP和SinoMed中关于上门护士岗位胜任力要素的研究,检索时限为建库至2022年12月,采用汇集性整合方法分析归纳结果。结果共纳入12篇文献、提炼157个主题、归纳9个类别、形成3个整合结果,共计33条胜任力条目。结论在评价上门护士岗位胜任力时,应从护士的个人特质与职业品质、专业综合能力和知识与技能方面进行综合评判,并纳入上门护士培养、考核与选拔中,以提高上门护士队伍的整体水平和服务质量。

关键词： “互联网+护理服务” 上门护士胜任力评价指标 Meta整合

乌司奴单抗在溃疡性结肠炎患者中的有效性和安全性:真实世界研究的荟萃分析

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胃肠病学和肝病学杂志 2024年第6期33卷 685-691页

作者：张朝柱刘宁纪粟敖建萍何明霞中国人民解放军陆军第九五一医院消化内科新疆库尔勒841000 中国人民解放军陆军第九五一医院心胸神经外科新疆库尔勒841000 中国人民解放军陆军第九五一医院介入医学中心新疆库尔勒841000

目的通过荟萃分析探究乌司奴单抗(Ustekinumab,UST)治疗溃疡性结肠炎(ulcerative colitis,UC)的安全性和有效性。方法检索Medline、EmBase、PubMed、万方数据库,对真实世界研究进行系统综述和Meta分析,评价UST治疗UC的安全性和有效性。... 详细信息

目的通过荟萃分析探究乌司奴单抗(Ustekinumab,UST)治疗溃疡性结肠炎(ulcerative colitis,UC)的安全性和有效性。方法检索Medline、EmBase、PubMed、万方数据库,对真实世界研究进行系统综述和Meta分析,评价UST治疗UC的安全性和有效性。结果共纳入11项研究(3384例患者),其中97.1%的UC患者曾接受生物制剂治疗。在12周诱导缓解期,UST治疗UC的临床应答率为61%,临床缓解率为34%,无激素缓解率为38%。维持缓解期24周时,临床缓解率为39%,无激素缓解率为40%。1年后,临床缓解率为37%,无激素缓解率为37%。UC患者中不良事件发生率为5%。发表偏倚评价结果显示无显著异质性。结论UST是治疗UC有效且安全的药物之一。对于中重度UC患者,特别是拒绝手术治疗的患者,考虑使用UST治疗可能会取得较好的疗效。

关键词：乌司奴单抗溃疡性结肠炎真实世界研究荟萃分析生物制剂

Progress and perspective on cotton breeding in Pakistan

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Journal of Cotton Research 2022年第4期5卷 25-41页

作者： SHAHZAD Kashif MUBEEN Iqra ZHANG Meng ZHANG Xuexian WU Jianyong XING chaozhu State Key Laboratory of Cotton Biology/Key Laboratory of Biological and Genetic Breeding of Cotton Ministry of Agriculture and Rural Affairs/Institute of Cotton ResearchChinese Academy of Agricultural ScienceAnyang 455000HenanChina Centre of Agricultural Biochemistry and Bio-technology University of AgricultureFaisalabad 38040Pakistan

Cotton is the prime natural fiber with economic significance *** farming and breeding have a long his-tory in *** development of high yielding upland cotton(Gossypium hirsutum)varieties gradually replaced the cultivation of diploid Gossypium *** change along with emergence of new epidemic diseases caused yield loss in recent *** biotic stress considerably reduced the performance and yield potential of ***-able breeding strategies are essential to generate useful genetic variations and to identify desired *** breeding has remarkably increased cotton yield and fiber quality,which has cultivated the NIAB-78,S-12,MNH‐786,and FH‐Lalazar like ***,this phenotypic selection based breeding method has low efficiency to pro-duce stress resilient *** efficiency of traditional breeding has significantly improved by the marker assisted selection *** in molecular genetics,bioinformatics analysis,genetic engineering,and genome sequencing have opened new technique routes for cotton *** addition,genetic improvement through quantitative trait loci,transcriptome,and CRISPR/Cas9 mediated genomic editing can provide suitable platform to improve the resistance to stresses induced by bollworms,cotton leaf curl virus,heat,drought,and *** approval of transgenic lines harboring triple gene Cry1Ac+Cry2A+GTG are critical for cotton *** review has critically discussed the progress and limitations of cotton breeding in Pakistan,and reviewed the utilization of novel genetic variations and selection tools for sustainable cotton production.

关键词： Cotton yield Biotic stress Abiotic stress Conventional breeding Molecular marker Genome selection

棉花CMS-D2和CMS-D8不育系线粒体全基因组比较分析

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棉花学报 2024年第1期36卷 28-38页

作者：葛李爽冯娟娟张梦郭立平戚廷香张学贤李永旗唐会妮乔秀琴邢朝柱吴建勇中国农业科学院棉花研究所/棉花生物育种与综合利用全国重点实验室/农业农村部棉花生物学与遗传育种重点实验室河南安阳455000

【目的】探究哈克尼西棉不育胞质(CMS-D2)和三裂棉不育胞质(CMS-D8)的不育系线粒体基因组之间的序列结构差异,为筛选鉴定不育相关基因奠定基础。【方法】根据D2A和D8A这2个不育系的线粒体基因组测序组装结果,使用Synteny and Rearrangem... 详细信息

【目的】探究哈克尼西棉不育胞质(CMS-D2)和三裂棉不育胞质(CMS-D8)的不育系线粒体基因组之间的序列结构差异,为筛选鉴定不育相关基因奠定基础。【方法】根据D2A和D8A这2个不育系的线粒体基因组测序组装结果,使用Synteny and Rearrangement Identifier(Sy RI)软件鉴定结构变异,用Plotsr可视化分析包含共线性及非共线性区域的重组变异位点。以D8A线粒体基因组注释结果为参考,用D2A线粒体基因组注释的开放阅读框(open reading frame,ORF)编码的氨基酸序列进行tblastn比对,筛选出D2A线粒体基因组中特有的ORF,并进行聚合酶链式反应(polymerase chain reaction,PCR)验证、相对表达量分析和生物信息学分析。【结果】D2A和D8A这2个不育系线粒体基因组之间存在2个部分重叠且相邻的倒易位区域。在D2A中发现17个特异的ORF,PCR验证了D2A中存在的6个特异ORF(orf114e、orf121b-1、orf121b-2、orf138b-2、orf186a-2和orf317a-2)。3~4 mm花蕾中orf121b-1和orf121b-2的相对表达量较高。orf114e、orf186a-2和orf317a-2具有典型的跨膜结构域和嵌合基因结构,符合不育基因的部分特征。【结论】D2A和D8A线粒体基因组间存在2个相邻的倒易位区域。D2A中存在6个特异的ORF,其中orf114e、orf186a-2和orf317a-2可能与棉花CMS-D2孢子体败育有关。

关键词：棉花细胞质雄性不育线粒体基因组孢子体不育配子体不育开放阅读框

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Numerical model of planar heterojunction organic solar cells

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Chinese Science Bulletin 2011年第19期56卷 2050-2054页

作者： MA chaozhu PENG YingQuan WANG RunSheng LI RongHua XIE HongWei WANG Ying XIE JiPeng YANG Ting Institute of Microelectronics of the School of Physical Science and Technology Lanzhou University Lanzhou 730000 China Key Laboratory for Magnetism and Magnetic Materials of the Ministry of Education Lanzhou University Lanzhou 730000 China Department of Basic Airforce Communication Sergeant Accademy Dalian 116600 China

We present a numerical study of the effects of the energy barrier between the lowest unoccupied molecular orbital of the acceptor layer and the cathode, the thicknesses of the donor layer and acceptor layer on the distributions of carrier density, the electric fields and the electric potentials of organic planar heterojunction solar cells. We obtained the quantitative dependencies of the distribution of carrier density, electric fields and the electric potentials on these quantities. The results provide a theoretical foundation for the experimental study of open-circuit organic planar heterojunction solar cells.

关键词：有机太阳能电池异质结数值模型平面载流子密度电势分布数值研究分子轨道

Late Cenozoic Geology and Paleo-environment Change in the Eastern Edge of Qinghai-Xizang Plateau

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Acta Geologica Sinica(English Edition) 2008年第5期82卷 959-966页

作者： ZHAO Zhizhong QIAO Yansong WANG shubing YAO Haitao WANG Yan LI chaozhu FU Jianli LIU Zongxiu LI Mingze MIAO Qi JIANG Fuchu Institute of Geomechanics Chinese Academy ofGeosciences Beijing 100081 China Open Laboratory of Lake Sediment and Environment Chinese Academy of Sciences Nanjing 210008 Jiangsu China

There are late Cenozoic lacustrine deposits and loess and red clay and moraines in eastern edge of the Qinghai-Xizang Plateau. Various genetic sediments recorded rich information on late Cenozoic paleo-environment changes. Xigeda lacustrine formed during 4.2 Ma B.P.-2.6 Ma B.P. There were 9 periodic warm-cold alternations. Eolian deposition in western Sichuan began at 1.15 Ma B.P. The loess-soil sequences recorded successively 14 paleo-monsoon climate cycles. Laterite in Chengdu plain recorded 5 stages of paleoclimatic stages since 1.13 Ma B.P. There was an old glacial period of 4.3 Ma B.P. in eastern Qinghai-Xizang Plateau. During Quaternary, there are 5 extreme paleoclimatic events corresponding with 5 glaciations.

关键词： Qinghai-Xizang Plateau Xigeda lacustrine Loess in western Sichuan Chengdu laterite Quaternary glaciations

The cotton mitochondrial chimeric gene orf610a causes male sterility by disturbing the dynamic balance of ATP synthesis and ROS burst

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The Crop Journal 2022年第6期10卷 1683-1694页

作者： Yongjie Zhang Yang Han Meng Zhang Xuexian Zhang Liping Guo Tingxiang Qi Yongqi Li Juanjuan Feng Hailin Wang Huini Tang Xiuqin Qiao Liangliang Chen Xiatong Song chaozhu Xing Jianyong Wu Zhengzhou Research Base State Key Laboratory of Cotton BiologySchool of Agricultural SciencesZhengzhou UniversityZhengzhou 450001HenanChina State Key Laboratory of Cotton Biology/Key Laboratory for Cotton Genetic Improvement Ministry of AgricultureInstitute of Cotton Research of Chinese Academy of Agricultural SciencesAnyang 455000HenanChina State Key Laboratory of Cotton Biology(Hebei Base) College of AgronomyHebei Agricultural UniversityBaoding 071001HebeiChina

Plant cytoplasmic male sterility(CMS)is maternally inherited and often manifested as aborted pollen development,but the molecular basis of abortion remains to be *** facilitate an investigation of CMS in cotton,the complete sequence of cotton mitochondrial(mt)genome for CMS-D2 line ZBA was *** mt genome was assembled as a single circular molecule with 634,036 bp in length.A total of 194 ORFs,36 protein-coding genes,six r RNAs,and 24 t RNAs were *** chimeric genes encoding hypothetical proteins with transmembrane domains were *** them,a previously unknown chimeric gene,orf610a,which is composed of atp1 and a 485-bp downstream sequence of unknown nature,was ***-PCR and q RT-PCR validation indicated that orf610a was expressed specifically in a sterile *** expression of orf610a in yeast resulted in excessive accumulation of reactive oxygen species and reduction in ATP content,in addition to inhibition of cellular *** *** overexpressing orf610a fused with a mitochondrial targeting peptide displayed partial male *** between ORF610a and the nuclear-encoded protein RD22 indicated an association between ORF610a and pollen *** feedback during transcriptional regulation between nuclear regulatory factors and the mt CMS gene may account for the male sterility of ZBA.

关键词： Cytoplasmic male sterility Mitochondrial genome Chimeric gene Reactive oxygen species ATP

基于转录组测序技术挖掘影响布莱凯特黑牛睾丸大小的功能基因

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华北农学报 2024年第1期39卷 191-200页

作者：梅艳芳刘瑞莉韩明轩张文琦陈振鹏王秀源苗秀萍肖超柱张任政董雅娟柏学进青岛农业大学动物科技学院山东青岛266109 黑牛种业技术创新中心山东青岛266109 山东兆福科技股份有限公司山东淄博256306

旨在通过对布莱凯特黑牛大小睾丸转录组数据进行分析,挖掘影响睾丸生殖功能的关键候选基因,探究睾丸大小对牛生殖功能维持的分子机制。采集12月龄布莱凯特黑牛睾丸,根据质量、长径和短径将其分为2组(大小睾丸组各3头)进行高通量转录组测... 详细信息

旨在通过对布莱凯特黑牛大小睾丸转录组数据进行分析,挖掘影响睾丸生殖功能的关键候选基因,探究睾丸大小对牛生殖功能维持的分子机制。采集12月龄布莱凯特黑牛睾丸,根据质量、长径和短径将其分为2组(大小睾丸组各3头)进行高通量转录组测序;以|log2(Fold Change)|≥1且P-value≤0.01为阈值筛选差异基因,对差异基因进行GO和KEGG功能富集分析,筛选出影响睾丸生殖功能的关键基因;对其进行CDS区克隆测序,并运用qRT-PCR、Western Blot、免疫组化等方法进行进一步研究。结果显示,差异表达基因共353个,其中114个基因在小睾丸中表达上调,239个基因表达下调。GO功能注释表明,差异基因参与代谢、发育、生殖等过程;KEGG富集分析显示,差异基因主要富集在Wnt信号传导、PI3K-Akt信号通路等与睾丸生殖功能相关的途径。最终筛选出睾丸生殖功能基因CCN4进行进一步研究。qRT-PCR和Western Blot结果显示,CCN4基因和蛋白在大睾丸中表达量显著低于小睾丸。免疫组化结果显示,CCN4蛋白主要在精细胞外的区域(特别是支持细胞)中表达,且大睾丸中的相对表达量显著低于小睾丸,推测其高表达会抑制支持细胞发育,从而影响睾丸生殖功能。综上,研究结果可为深入开展牛睾丸大小对生殖功能影响的分子机制研究提供基础资料。

关键词：牛大小睾丸转录组差异基因 CCN4

Cytochrome P450 mono-oxygenase CYP703A2 plays a central role in sporopollenin formation and ms5ms6 fertility in cotton

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Journal of Integrative Plant Biology 2022年第10期64卷 2009-2025页

作者： Huanhuan Ma Yuanlong Wu Ruiling Lv Huabin Chi Yunlong Zhao Yanlong Li Hongbo Liu Yizan Ma Longfu Zhu Xiaoping Guo Jie Kong Jianyong Wu chaozhu Xing Xianlong Zhang Ling Min National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070China College of Biology and Agricultural Resources Huanggang Normal UniversityHuanggang 438000China College of Plant Science and Technology Huazhong Agricultural UniversityWuhan 430070China Institute of Economic Crops Xinjiang Academy of Agricultural SciencesXinjiang 830091China State Key Laboratory of Cotton Biology Institute of Cotton Research of Chinese Academy of Agricultural SciencesAnyang 455000China

The double-recessive genic male-sterile(ms)line ms5 ms6 has been used to develop cotton(Gossypium hirsutum)hybrids for many years,but its molecular-genetic basis has remained ***,we identified the Ms5 and Ms6 loci through map-based cloning and confirmed their function in male sterility through CRISPR/Cas9 gene ***5 and Ms6 are highly expressed in stages 7–9 anthers and encode the cytochrome P450 mono-oxygenases CYP703A2-A and *** ms5 mutant carries a single-nucleotide C-to-T nonsense mutation leading to premature chain termination at amino acid 312(GhCYP703A2-A^(312aa)),and ms6 carries three nonsynonymous substitutions(D98E,E168K,and G198R)and a synonymous mutation(L11L).Enzyme assays showed that GhCYP703A2 proteins hydroxylate fatty acids,and the ms5(GhCYP703A2-A^(312aa))and ms6(GhCYP703A2-D^(D98E,E168K,G198R))mutant proteins have decreased enzyme *** and lipidomic analyses showed that in ms5 ms6 plants,C12–C18 free fatty acid and phospholipid levels are significantly elevated in stages 7–9 anthers,while stages 8–10 anthers lack sporopollenin fluorescence around the pollen,causing microspore degradation and male ***,our characterization uncovered functions of GhCYP703A2 in sporopollenin formation and fertility,providing guidance for creating male-sterile lines to facilitate hybrid cotton production and therefore exploit heterosis for improvement of cotton.

关键词： cotton cytochrome P450 mono-oxygenase doublerecessive genic male sterility sporopollenin formation