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现代农药 2024年第2期23卷 26-32页

作者：曾哲政罗朝喜华中农业大学果蔬园艺作物种质创新与利用全国重点实验室武汉430070 华中农业大学植物科技学院湖北省作物病害监测及安全控制重点实验室武汉430070

概述了我国主要的桃病害,介绍了在桃病害防治中使用的药剂及其应用技术。针对当前桃病害化学防治中出现的抗药性问题,系统总结和分析了相关研究成果,以期为桃病害监测和绿色防控提供参考,实现对桃病害更加高效、安全的防控。

关键词：桃树病害化学防治抗药性

华中农业大学“三田”实践教学的信息化实践与探索

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科教导刊 2024年第11期 75-77页

作者：袁金展李海霞罗朝喜华中农业大学作物学国家级实验教学示范中心湖北武汉430070

随着现代农业的快速发展,植物生产类专业生产实习需要及时调整教学模式,以适应现代农业技术的发展。文章介绍了华中农业大学近年来植物生产类相关专业“三田”实习的信息化建设与实践:“三田”基地信息化改造、“三田”实习课程改革、... 详细信息

随着现代农业的快速发展,植物生产类专业生产实习需要及时调整教学模式,以适应现代农业技术的发展。文章介绍了华中农业大学近年来植物生产类相关专业“三田”实习的信息化建设与实践:“三田”基地信息化改造、“三田”实习课程改革、实践实习模式创新。同时,结合学校实际情况提出创新“三田”实习信息化实践教学,升级改造课程内容及组织形式,同时提升师资信息化水平,从而使“三田”实习向智慧化方向发展。

关键词：植物生产类 “三田”实习信息化

bZIP Transcription Factor UvATF21 Mediates Vegetative Growth,Conidiation,Stress Tolerance and Is Required for Full Virulence of Rice False Smut Fungus Ustilaginoidea virens

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Rice science 2023年第1期30卷 50-57页

作者： LIU Yueran QU Jinsong WANG Yufu YIN Weixiao luo chaoxi Hubei Key Laboratory of Plant Pathology/College of Plant Science and Technology Huazhong Agricultural UniversityWuhan 430070China

bZIP proteins are widely distributed in eukaryotic organisms and regulate a diverse range of physiological *** bZIP proteins have previously been identified in Ustilaginoidea ***,the biological roles of these bZIP proteins in this pathogen are still ***,one of these bZIP protein coding genes,UvATF21,was functionally *** deletion of UvATF21resulted in reduced conidiation and pathogenicity despite of the increased vegetative *** deletion mutants also significantly decreased the sensitivity to osmotic and oxidative ***,deletion of UvATF21 exhibited different performances to cell wall integrity *** results indicated that UvATF21 played crucial roles in vegetative growth,conidiation,stress response,and full virulence in ***.

关键词： Ustilaginoidea virens bZIP protein conidiation stress response pathogenicity

MfCdtf1基因对桃褐腐病菌生长、产孢和致病力的影响

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植物保护学报 2024年第3期51卷 672-683页

作者：杨静雅曾哲政肖媛玲蔡民政吴沛珊魏闻恺阴伟晓罗朝喜华中农业大学果蔬园艺作物种质创新与利用全国重点实验室武汉430070 华中农业大学植物科技学院湖北省作物病害监测及安全控制重点实验室武汉430070

为探究桃褐腐病菌实生链核盘菌Monilinia fructicola环磷酸腺苷依赖性转录因子(cyclic adenosine monophosphate dependent transcription factor,Cdtf)在侵染植物寄主过程中发挥的作用,以野生型菌株Bmpc7为研究对象,通过基因组学和侵... 详细信息

为探究桃褐腐病菌实生链核盘菌Monilinia fructicola环磷酸腺苷依赖性转录因子(cyclic adenosine monophosphate dependent transcription factor,Cdtf)在侵染植物寄主过程中发挥的作用,以野生型菌株Bmpc7为研究对象,通过基因组学和侵染早期转录组学分析预测Cdtf1基因的结构及功能,对该基因进行敲除并对敲除转化子和回补转化子进行表型和抗逆性测定。结果显示,MfCdtf1在桃褐腐病菌侵染早期上调表达明显,接种后1 h和24 h时MfCdtf1的表达量显著上升,表明MfCdtf1参与调控桃褐腐病菌的侵染和致病。对3个纯合MfCdtf1敲除转化子和3个杂合回补转化子的表型进行观察,发现MfCdtf1基因的缺失使桃褐腐病菌的菌丝生长速率和致病力显著降低,产孢能力和色素合成能力丧失。甘油、H_(2)O_(2)、十六烷基三甲基溴化铵、十二烷基苯磺酸钠、CaCl_(2)、KCl和NaCl等多种外源胁迫对敲除转化子ΔMfCdtf1的生长抑制率显著低于对野生型菌株Bmpc7的生长抑制率,表明MfCdtf1的缺失降低了桃褐腐病菌对外源胁迫的敏感性。

关键词：桃褐腐病实生链核盘菌 MfCdtf1基因致病机制外源胁迫抗逆性

黄马酊联合雷公藤多苷片治疗免疫性小血管炎慢性创面临床观察

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亚太传统医药 2024年第4期20卷 68-72页

作者：何姚姚陈岚罗朝熙熊贵莉曾充聪银曼羽曹艳尹千璐重庆市中医院重庆400021

目的:观察黄马酊联合雷公藤多苷片治疗免疫性小血管炎慢性创面的临床疗效。方法:42例免疫性小血管炎患者按随机数字表分成对照组21例和观察组21例。对照组给予基础常规治疗和醋酸泼尼松片,创面局部予以黄马酊治疗;观察组在对照组基础上... 详细信息

目的:观察黄马酊联合雷公藤多苷片治疗免疫性小血管炎慢性创面的临床疗效。方法:42例免疫性小血管炎患者按随机数字表分成对照组21例和观察组21例。对照组给予基础常规治疗和醋酸泼尼松片,创面局部予以黄马酊治疗;观察组在对照组基础上加雷公藤多苷片口服。结果:两组患者治疗后创面面积均减少,愈合率提高,伯明翰血管炎性活动性评分均降低,而观察组优于治疗组,差异具有统计学意义(P<0.01)。两组患者治疗后hs-CRP、ESR均下降,血清VEGF水平增加,差异具有统计学意义(P<0.05),观察组hs-CRP、ESR均低于对照组,血清VEGF水平高于对照组,差异具有统计学意义(P<0.01)。结论:黄马酊联合雷公藤的使用能降低免疫性小血管炎活动性,减轻炎症反应,促进血管生成,提高创面愈合率。

关键词：黄马酊雷公藤多苷片免疫性小血管炎慢性创面

UvHP1 Regulates Growth,Sporulation,Hydrophobicity and Pathogenesis in Ustilaginoidea virens

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UvHP1 Regulates Growth,Sporulation,Hydrophobicity and Pathog...

Ustilaginoidea virens modulates lysine 2-hydroxyisobutyrylation in rice flowers during infection

中国植物病理学会2023年学术年会

作者： YAN Jiali WANG Yufu luo chaoxi YIN Weixiao Hubei Key Laboratory of Plant Pathology College of Plant Science and Technology Huazhong Agricultural University

In recent years,the area of occurrence and severity of rice false smut cussed by Ustilaginoidea virens has been increasing in China,which has been one of the major rice *** fungal disease causes significant yield losses and poses a threat to human and animal health through the production of lungal *** research has been reported on the pathogenesis of *** and the mining of resistance resources to *** analysis revealed that UvHP1 exhibited up-regulated expressionat early infection ***,the function of UvHP1 was elucidated in ***,complemented and overexpression transformants were obtained by PEG-mediated protoplast transformation and their growth,spore production,hydrophobicity of mycelium and pathogenicity were *** growth rate of knockout and overexpression transformants was increased,indicating that the UvHP1 regulates the growth of *** addition,sporulation and spore germination rate of the knockout and overexpression transformants were significantly lower than wild ***,knockout transformants were more easily penetrated by aniline blue,whereas overexpression transformants were more difficult to be penetrated,suggesting that UvHP1 regulates the hydrophobic;function of *** UvHP1 knockout and overexpression transformants showed reduced pathogenesis,indicating that the UvHP1 gene contributes to the pathogenesis of *** results suggest that UvHP1 is involved in growth,sporulation,mycelial hydrophobicity,and pathogenesis.

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Journal of Integrative Plant Biology 2021年第10期63卷 1801-1814页

作者： Xiaoyang Chen Qiutao Xu Yuhang Duan Hao Liu Xiaolin Chen Junbin Huang chaoxi luo Dao-Xiu Zhou Lu Zheng State Key Laboratory of Agricultural Microbiology/Hubei Key Laboratory of Plant Pathology Huazhong Agricultural UniversityWuhan 430070China National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070China Institute of Plant Science Paris-Saclay(IPS2) CNRSINRAEUniversity Paris-SaclayOrsay 91405France

The post-translational modification lysine 2-hydroxyisobutyrylation(K_(hib))plays an important role in gene transcription,metabolism,and enzymatic activity.K_(hib)sites have been identified in rice(Oryza sativa).However,the K_(hib)status of proteins in rice flowers during pathogen infection remains ***,we report a comprehensive identification of K_(hib)-modified proteins in rice flowers,and the changes in these proteins during infection with the fungal pathogen Ustilaginoidea *** using a tandem mass tag-based quantitative proteomics approach,we identified 2,891 K_(hib)sites on 964 proteins in rice *** data demonstrated that 2-hydroxyisobutyrylated proteins are involved in diverse biological processes.K_(hib)levels were substantially reduced upon infection with *** immunoprecipitation polymerase chain reaction(PCR)and reverse transcription quantitative PCR analyses revealed that histone K_(hib)is involved in the expression of disease-resistance *** importantly,most quantified sites on core histones H3 were downregulated upon *** *** addition,the histone deacetylases HDA705,HDA716,SRT1,and SRT2 are involved in the removal of K_(hib)marks in ***705 was further confirmed to negatively regulate rice disease resistance to pathogens ***,Magnaporthe oryzae,and Xanthomonas oryzae ***(Xoo).Our data suggest that *** could modulate K_(hib)in rice flowers during infection.

关键词： histone deacetylases infection Khib Ustilaginoidea virens

MfMel1基因参与调控桃褐腐病菌对桃果实的致病力

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植物保护学报 2023年第2期50卷 380-390页

作者：谷文倩阴伟晓罗朝喜华中农业大学果蔬园艺作物种质创新与利用全国重点实验室武汉430070 华中农业大学植物科技学院湖北省作物病害监测及安全控制重点实验室武汉430070

为明确MfMel1基因在桃褐腐病菌Monilinia fructicola中的功能,利用生物信息学软件对该基因的结构、所编码蛋白的保守结构域以及进化关系进行分析;通过遗传转化方法对该基因进行敲除,并进行表型分析。结果显示,敲除转化子△MfMel1菌株的... 详细信息

为明确MfMel1基因在桃褐腐病菌Monilinia fructicola中的功能,利用生物信息学软件对该基因的结构、所编码蛋白的保守结构域以及进化关系进行分析;通过遗传转化方法对该基因进行敲除,并进行表型分析。结果显示,敲除转化子△MfMel1菌株的菌丝生长速率、孢子萌发率与野生型菌株Bmpc7均无显著差异,但产孢量显著降低。△MfMel1菌株在0.01%SDS、600μg/mL刚果红、150 g/L甘油、200 g/L蔗糖、6 mmol/L H_(2)O_(2)、4%NaCl和1.2 mmol/L山梨醇培养基上的生长速率、菌丝形态均无显著变化,但对200 g/L葡萄糖的渗透胁迫敏感性增强。敲除转化子△MfMel1菌株的α-半乳糖苷酶活性明显降低,对桃果实的致病力显著下降。表明MfMel1基因在桃褐腐病菌中可能通过参与调控α-半乳糖苷酶酶活,从而参与病原菌的致病。

关键词：桃褐腐病菌 MfMel1基因糖苷水解酶侵染机制 α-半乳糖苷酶

Identification and Characterization of the Nepl-like Protein UvNLP in Rice False Smut Fungus Ustilaginoidea virens

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Identification and Characterization of the Nepl-like Protein...

Comprehensive identification of lysine 2-hydroxyisobutyrylated proteins in Ustilaginoidea virens reveals the involvement of lysine 2-hydroxyisobutyrylation in fungal virulence

中国植物病理学会2023年学术年会

作者： WANG Yufu Qu Jinsong XIE Songlin WANG Zuoqian YIN Liangfen YIN Weixiao luo chaoxi Key Laboratory of Crop Disease Monitoring & Safety Control in Hubei Province College of Plant Science and Technology Huazhong Agricultural University

The necrosis-and ethylene-inducing peptide 1(Nepl)-like proteins(NLPs) are a broadly distributed superfamily of protiens in plant-associated microorganisms,including bacteria,fungi,and *** are considered to be important virulence factors,and some have been well ***,the role of NLPs in Ustilaginoidea virens,the agent of rice false smut fungus,remains to be *** this study,a protein containing NLP-specific NPP1 domain was identified in *** and named ***;analysis revealed that UvNLP was a type 2 NLP,and realtime PCR revealed that UvNLP was highly expressed at 3 days post-inoculation.A yeast secretion assay demonstrated that UvNLP contained a functional signal peptide(SP).Furthermore,transient expression of UvNLP in Nicotiana benthamiana induced weak cell death,and replacement of the SP failed to enhance cell ***-time PCR indicated that UvNLP induced the expression of plant defence *** conclusion,we identified a secreted protein UvNLP in *** that was infection related and could inducted plant cell death and defence *** study providesd evidence that NLPs act as proteinaceous MAMPs,giving insight into the elucidation of the pathogenicity mechanism of *** UvNLP might be used as a potential plant defence inducer for disease control in practice.

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Journal of Integrative Plant Biology 2021年第2期63卷 409-425页

作者： Xiaoyang Chen Xiabing Li Pingping Li Xiaolin Chen Hao Liu Junbin Huang chaoxi luo Tom Hsiang Lu Zheng Hubei Key Laboratory of Plant Pathology Huazhong Agricultural UniversityWuhan 430070China School of Environmental Sciences University of GuelphGuelphOntarioN1G 2W1Canada

Lysine 2-hydroxyisobutyrylation(Khib) is a newly identified post-translational modification(PTM) that plays important roles in transcription and cell proliferation in eukaryotes. However, its function remains unknown in phytopathogenic fungi. Here,we performed a comprehensive assessment of Khibin the rice false smut fungus Ustilaginoidea virens, using Tandem Mass Tag(TMT)-based quantitative proteomics approach. A total of 3 426 Khibsites were identified in 977 proteins, sugg esting that Khibis a common and complex PTM in U. virens. Our data demonstrated that the2-hydroxyisobutyrylated proteins are involved in diverse biological processes. Network analysis of the modified proteins revealed a highly interconnected protein network that included many well-studied virulence factors. We confirmed that the Zn-binding reduced potassium dependency3-type histone deacetylase(UvRpd3) is a major enzyme that removes 2-hydroxyisobutyrylation and acetylation in U. virens. Notably, mutations of Khib sites in the mitogen-activated protein kinase(MAPK)UvSlt2 significantly reduced fungal virulence and decreased the enzymatic activity of UvSlt2. Molecular dynamics simulations demonstrated that 2-hydroxyisobutyrylation in UvSlt2 increased the hydrophobic solvent-accessible surface area and thereby affected binding between the UvSlt2 enzyme and its substrates. Our findings thus establish Khibas a major post-translational modification in U. virens and point to an important role for Khibin the virulence of this phytopathogenic fungus.

关键词： Khib Ustilaginoidea virens virulence