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Overexpression of Stella improves the efficiency of nuclear transfer reprogramming

Journal of Genetics and Genomics 2017年第7期44卷 363-366页

作者： Leyun Wang Fei Teng Xuewei Yuan chao Liu qiang Wang Yufei Li Tongtong Cui Tianda Li Zhonghua Liu qi Zhou College of Life Science Northeast Agricultural UniversityHarbin 150030China State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101 China University of Chinese Academy of Sciences Beijing 100049China

In mammalians, the state of a somatic cell can be reversed from the terminal state to the totipotent state by means of somatic cell nuclear transfer （SCNT）（Gurdon, 1962） or induced pluripotent stem cells （iPSCs）（Takahashi and Yamanaka, 2006）. The DNA methylation and transcriptome profiles of embryonic stern cells （ESCs） derived from SCNT embryos （NT-ESCs） correspond closely to those of ESCs derived from in vitro fertilization embryos （IVF- ESCs）. In contrast, iPSCs differ from both NT-ESCs and IVF-ESCs in that they retain the residual DNA methylation patterns of their parental somatic cells. As SCNT can be used to faithfully reprogram human somatic cells to pluripotency, it is ideal for cell replacement therapies （Ma et al., 2014）. Following the successful production of the first human NT-ESCs （Tachibana et al., 2013） and the later gen- eration of human NT-ESCs based on cells from elderly adults or pa- tient cells （Chung et al., 2014; Yamada et al., 2014）, a version of the SCNT technique for human therapeutics comes closer to reality. However, no matter what animal species or donor cell types are used in the cloned process, the cloning efficiency remains undesir- able. Besides, there are many phenotypic abnormalities in cloned animals, containing frequent embryonic and perinatal death and placentomegaly, and the underlying mechanisms remain unclear （Yang et al, 2007）.

关键词： of cell as that Overexpression of Stella improves the efficiency of nuclear transfer reprogramming TSA were

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Scientific Meaning and Significance of the Right to Development

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The Journal of Human Rights 2015年第4期14卷 307-311页

作者：李步云 qi jun the Human Rights Institute Guangzhou University

The right to life and the right to development are primary rights of *** is a basic view of Chinese human *** many foreign scholars refuse to consider the right to development as part of human rights;while,many domestic scholars hold different opinions towards the meaning of the right to *** Clarify the precise meaning of the right to development is

关键词： Scientific Meaning and Significance of the Right to Development

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黄土高原半干旱雨养农业区冬小麦气候适宜度评价——以通渭县为例

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中国农学通报 2024年第17期40卷 73-80页

作者：焦润安李旭华齐月雷俊苟志文臧亚军薛潇甘肃省通渭县气象局甘肃通渭743300 定西市农业科学研究院甘肃定西743000 中国气象局兰州干旱气象研究所/甘肃省干旱气候变化与减灾重点实验室/中国气象局干旱气候变化与减灾重点开放实验室兰州730020 定西市气象局甘肃定西743000 兰州资源环境职业技术大学兰州730021

气候适宜度可以表征气候条件对作物生长发育的影响程度。利用黄土高原半干旱区通渭县近40 a气象资料和农业气象观测资料,基于模糊数学方法,根据冬小麦各发育时段对温度、水分、光照的需求,对黄土高原半干旱雨养农业区冬小麦全生育期气... 详细信息

气候适宜度可以表征气候条件对作物生长发育的影响程度。利用黄土高原半干旱区通渭县近40 a气象资料和农业气象观测资料,基于模糊数学方法,根据冬小麦各发育时段对温度、水分、光照的需求,对黄土高原半干旱雨养农业区冬小麦全生育期气候适宜度进行评价。结果表明:通渭县近40 a冬小麦全生育期温度适宜度为0.58,停止生长—返青期的温度适宜度最高,达0.95,三叶期—停止生长的温度适宜度低,仅为-0.42,年际间差异小,全生育期温度适宜度以0.019/10 a的速率上升;水分适宜度为0.46,乳熟期—成熟期的水分适宜度最高,为0.66,其次为返青期—拔节期,为0.63,年际间差异大,全生育期水分适宜度以0.017/10 a的速率上升;光照适宜度为0.62,三叶期—停止生长和返青期—拔节期的光照适宜度最高,为0.68,乳熟期—成熟期最低,为0.51,全生育期光照适宜度以0.011/10 a的速率上升;综合适宜度为0.54,返青期—拔节期的综合适宜度最高,为0.70,三叶期—停止生长最低,为-0.36,全生育期综合适宜度以0.014/10 a的速率上升。冬小麦产量与抽穗期—乳熟期温度适宜度、乳熟期—成熟期水分适宜度、乳熟期—成熟期综合适宜度呈显著相关。气候适宜度能够较准确地反映出黄土高原半干旱雨养农业区冬小麦生育期内气象条件的优劣变化。

关键词：黄土高原半干旱区冬小麦气候适宜度评价指标

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A Novel Acetyl Migration

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Chinese Chemical Letters 1998年第7期9卷 619-620页

作者： Xi Han WU A. A. Haji Wei LU jun chao CAI (Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 200031) Shanghai Institute of Materia Medica Chinese Academy of Sciences Shanghai 200031 China

In our effort to asymmetric synthesis of R-(+)-shikonin, a novel acetyl migration was found, and the mechanism of the migration was considered to be mediated by a 6 atom-ring intermediate.

关键词： acetyl migration intermediate

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肾移植术后耶氏肺孢子菌肺炎的临床表现及诊治分析

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临床肺科杂志 2024年第3期29卷 331-336页

作者：李叶青王鹤黄光伟李世荣胡岚王超齐文杰首都医科大学附属北京友谊医院感染内科北京100050

目的分析探讨肾移植术后耶氏肺孢子菌肺炎(pneumocystis jiroveci pneumonia,PJP)的发病情况及临床诊治情况。方法回顾性分析2015年1月至2021年7月首都医科大学附属北京友谊医院收治的肾移植术后PJP患者的临床表现、实验室及影像学结果... 详细信息

目的分析探讨肾移植术后耶氏肺孢子菌肺炎(pneumocystis jiroveci pneumonia,PJP)的发病情况及临床诊治情况。方法回顾性分析2015年1月至2021年7月首都医科大学附属北京友谊医院收治的肾移植术后PJP患者的临床表现、实验室及影像学结果以及诊治过程。结果本研究共收录肾移植术后耶氏肺孢子菌肺炎患者34例,其中男性21例,女性13例。感染时间为肾移植术后8.5(6.0,18.0)月;主要临床表现为发热31例(占91.2%)、干咳14例(占41.2%)及喘憋13例(占38.2%);相关感染指标中(1,3)-β-D葡聚糖[147.7(60.0,258.7)pg/mL]、乳酸脱氢酶[(393.94±107.94)U/L]及C-反应蛋白[30.5(12.8,57.5)mg/L]明显升高,与出院时结果差异有显著统计学意义(P<0.01);病原学检查以痰耶氏肺孢子菌PCR检测为主,阳性率为56.7%,肺泡灌洗液(BALF)PCR、痰液、肺泡灌洗液及血液的宏基因组二代测序(macrogenomic next-generation sequencing,mNGS)检测阳性率为100%;研究中有25例(占73.5%)患者使用标准剂量复方磺胺甲噁唑(trimethoprim-sulfamethoxazole,TMP-SMZ)抗感染治疗,9例(占26.5%)病变相对严重或合并肾功能不全者使用低剂量TMP-SMZ联合卡泊芬净,两组治愈率均为100%;治疗中共出现骨髓抑制12例(占35.3%)、Ⅰ型呼吸衰竭11例(占32.4%)、急性肾损伤9例(占26.5%)、急性肝损伤3例(占8.8%),两组间各并发症发生率无统计学差异(P=0.439,1.000,0.386,0.549)。结论可疑的临床及肺CT表现结合(1,3)-β-D葡聚糖的升高及痰耶氏肺孢子菌PCR检测有利于PJP的早期诊断,BALF的PCR及相关标本mNGS检测可协助提高诊断阳性率,标准剂量TMP-SMZ或低剂量联合卡泊芬净对PJP疗效值得肯定,但骨髓抑制、肝肾功能损伤等合并症仍需关注。

关键词：肾移植耶氏肺孢子菌肺炎临床表现诊断治疗合并症

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海上油田热采用合金管线断裂失效研究

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全面腐蚀控制 2024年第4期38卷 109-114页

作者：万芬孙君辛野吴婷冯佳琦和一帆中海油能源发展股份有限公司工程技术分公司天津300452 中海油常州涂料化工研究院有限公司天津300452

某海上油田注热作业时825合金液控管线及光纤管线断裂失效,对合金管线进行宏观检查、微观观测及理化性能分析,并对收集的产物进行分析,判断合金管断裂失效为疲劳断裂与应力腐蚀,开裂起源于外表面点蚀坑,并提出失效控制措施。

关键词： 825合金疲劳断裂应力腐蚀失效分析

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AVP(4-8) Improves Cognitive Behaviors and Hippocampal Synaptic Plasticity in the APP/PS1 Mouse Model of Alzheimer’s Disease

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Neuroscience Bulletin 2020年第3期36卷 254-262页

作者： Xiumin Zhang Fang Zhao Chenfang Wang jun Zhang Yu Bai Fang Zhou Zhaojun Wang Meina Wu Wei Yang junhong Guo Jinshun qi Department of Neurology First HospitalShanxi Medical UniversityTaiyuan 030001China Department of Physiology Key laboratory of Cellular PhysiologyMinistry of EducationShanxi Medical UniversityTaiyuan 030001China

Memory deficits with aging are related to the neurodegeneration in the brain, including a reduction in arginine vasopressin(AVP) in the brain of patients with Alzheimer’s disease(AD). AVP(4-8), different from its precursor AVP, plays memory enhancement roles in the CNS without peripheral side-effects. However, it is not clear whether AVP(4-8) can improve cognitive behaviors and synaptic plasticity in the APP/PS1 mouse model of AD. Here, we investigated for the first time the neuroprotective effects of AVP(4-8) on memory behaviors and in vivo long-term potentiation(LTP) in APP/PS1-AD *** results showed that:(1) APP/PS1-AD mice had lower spontaneous alternation in the Y-maze than wild-type(WT) mice, and this was significantly reversed by AVP(4-8);(2) the prolonged escape latency of APP/PS1-AD mice in the Morris water maze was significantly decreased by AVP(4-8), and the decreased swimming time in target quadrant recovered significantly after AVP(4-8) treatment;(3) in vivo hippocampal LTP induced by high-frequency stimulation had a significant deficit in the AD mice, and this was partly rescued by AVP(4-8);(4) AVP(4-8)significantly up-regulated the expression levels of postsynaptic density 95(PSD95) and nerve growth factor(NGF)in the hippocampus of AD mice. These results reveal the beneficial effects of AVP(4-8) in APP/PS1-AD mice,showing that the intranasal administration of AVP(4-8)effectively improved the working memory and long-term spatial memory of APP/PS1-AD mice, which may be associated with the elevation of PSD95 and NGF levels in the brain and the maintenance of hippocampal synaptic plasticity.

关键词： AVP(4-8) APP/PS1 transgenic mice Cognitive behavior Synaptic plasticity In vivo hippocampal LTP

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Thermal safety of dendritic lithium against non-aqueous electrolyte in pouch-type lithium metal batteries

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Journal of Energy Chemistry 2022年第9期31卷 158-165,I0005页

作者： Feng-Ni Jiang Shi-Jie Yang Xin-Bing Cheng Peng Shi jun-Fan Ding Xiang Chen Hong Yuan Lei Liu Jia-qi Huang qiang Zhang College of Chemistry and Chemical Engineering Taiyuan University of TechnologyTaiyuan 030024ShanxiChina Beijing Key Laboratory of Green Chemical Reaction Engineering and Technology Department of Chemical EngineeringTsinghua UniversityBeijing 100084China Key Laboratory of Energy Thermal Conversion and Control of Ministry of Education School of Energy and EnvironmentSoutheast UniversityNanjing 211189JiangsuChina Advanced Research Institute of Multidisciplinary Science Beijing Institute of TechnologyBeijing 100081China

A quantitative relationship between safety issues and dendritic lithium(Li) has been rarely investigated yet. Herein the thermal stability of Li deposits with distinct surface area against non-aqueous electrolyte in pouch-type Li metal batteries is probed. The thermal runaway temperatures of Li metal batteries obtained by accelerating rate calorimeter are reduced from 211 ℃ for Li foil to 111 ℃ for cycled *** initial exothermic temperature is reduced from 194 ℃ for routine Li foil to 142 ℃ for 49.5 m~2g^(-1) dendrite. Li with different specific surface areas can regulate the reaction routes during the temperature range from 50 to 300 ℃. The mass percent of Li foil and highly dendritic Li reacting with ethylene carbonate is higher than that of moderately dendritic Li. This contribution can strengthen the understanding of the thermal runaway mechanism and shed fresh light on the rational design of safe Li metal batteries.

关键词： Pouch-type cell Battery safety Lithium metal anode Lithium dendrite growth Thermal runaway

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基于滋味-成分关联分析探究桔梗苦味物质基础

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时珍国医国药 2024年第7期35卷 1767-1772页

作者：阿丽娅·阿卜杜热依木李潮魏娜孙庆许嘉文孙秀岩张彦青解军波天津中医药大学中药学院天津301617 天津商业大学生物技术与食品科学学院天津300134

目的通过电子舌与高效液相色谱法,结合分子对接技术初步明确桔梗苦味物质基础。方法收集桔梗样品26批,运用电子舌技术对其滋味进行量化,同时用HPLC法测定桔梗中8种单体成分含量,通过聚类分析、正交-偏最小二乘分析和斯皮尔曼相关性分析... 详细信息

目的通过电子舌与高效液相色谱法,结合分子对接技术初步明确桔梗苦味物质基础。方法收集桔梗样品26批,运用电子舌技术对其滋味进行量化,同时用HPLC法测定桔梗中8种单体成分含量,通过聚类分析、正交-偏最小二乘分析和斯皮尔曼相关性分析对获取的滋味和单体成分含量进行处理,比较不同产地桔梗滋味与有效成分含量差异,筛选出与桔梗苦味相关的化学成分,并利用分子对接技术验证苦味物质与人源苦味受体TAS2R14的结合力。结果桔梗的滋味及有效成分含量在南北地区间存在差异,桔梗的苦味与党参炔苷、桔梗皂苷G_1、桔梗皂苷E、桔梗皂苷D_3、桔梗皂苷D_2、桔梗皂苷D、远志皂苷D_2、远志皂苷D存在显著正相关性,分子对接结果显示所测的8种单体成分与TAS2R14的有较好的结合力。结论初步阐明了桔梗的苦味物质基,为桔梗道地性评价方法提供了科学依据。

关键词：桔梗电子舌苦味物质基础分子对接 TAS2R14

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Effects of Bushen Yiqi Huoxue Decoction in Treatment of Patients with Diminished Ovarian Reserve:A Randomized Controlled Trial

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Chinese Journal of Integrative Medicine 2022年第3期28卷 195-201页

作者： ZHOU Jing PAN Xin-yao LIN Jin ZHOU qi LAN Li-kun ZHU jun DUAN Ru WANG Lan SUN Yan WANG Ling Laboratory for Reproductive Immuno logy Hospital&Institute of Obstetrics and Gyn ecologyShan ghai Medical CollegeFudan UniversityShanghai(200000)China Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases Shanghai(200000)China Academy of Integrative Medicine of Fudan University Shanghai(200000)China Yangpu Hospital of Traditional Chinese Medicine Shanghai(200000)China Second Affiliated Hospital of Hexi University ZhangyeGansu Province(743000)China Department of Obstetrics and Gyn ecology Wenling People's HospitalWenzhou Medical UniversityWenzhouZhejiang Provinee(325000)China Regulatory Commissi on Office of the New Cooperative Medical Scheme Eryuan CountyDaliYunnan Province(671000)China

Objective:To explore the therapeutic effect of Bushen Yiqi Huoxue Decoction BYHD)in patients with diminished ovarian reserve(DOR).Methods:A total of 180 patients with DOR diagnosed from December2013 to December 2014 were equally assigned into progynova and duphaston(E+D)group,Zuogui Pill group and BYHD group with 60 cases in each by computerized *** received E+D,Zuogui Pill or BYHD for 12 months,*** stimulating hormone(FSH),luteinizing hormone(LH),estradiol(E_(2)),anti-Mullerian hormone(AMH),antral follicle count(AFC),ovarian volume,endometrial thickness,and the resistance indices(Rls)of ovarian arteries and uterine arteries were observed before and after ***:Nine women(4 from the E+D group,3 from the Zuogui Pill group,and 2 from the BYHD group)withdrew from the *** 6 months,Zuogui Pill and BYHD significantly decreased FSH and LH and increased endometrial thickness and AMH(all Pqi and activating blood circulation may be a promising therapeutic strategy for DOR.

关键词： Bushen Yiqi Huoxue Decoction diminished ovarian reserve randomized controlled trial Chinese medicine

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