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Wastewater surveillance and an automated robot:effectively tracking SARS-CoV-2 transmission in the post-epidemic era

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National Science Review 2023年第6期10卷 86-95页

作者： Guanyong Ou Yuxuan Tang Shiyu Niu Liwen Wu Shaxi Li Yang Yang Jun Wang Yun Peng Chuanfu huang Wei Hu Qinghua Hu Yinghui Li Yang Ping chao Lin Boping Yu Qi Han Yabin Hao Zhiguang Luo Wende Tian Han Zhang Yingxia Liu National Clinical Research Center for Infectious Disease State Key Discipline of Infectious Disease The Third People's Hospital of Shenzhen Second Hospital Affiliated to Southern University of Science and Technology School of Medicine Southern University of Science and Technology International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education Institute of Microscale Optoelectronics College of Physics and Optoelectronic Engineering Shenzhen University Shenzhen Longhua Drainage Co. Ltd. Shenzhen Center for Disease Control and Prevention Microbiology Laboratory Shenzhen Center for Disease Control and Prevention Power China Eco-Environmental Group Co. Ltd. Shenzhen Water Planning & Design Institute Co. Ltd. Shenzhen Academy of Environmental Sciences School of Civil and Environmental Engineering Harbin Institute of Technology (Shenzhen) 1Institute of Solid Wastes and Physical Environment Research Shenzhen Academy of Environmental Sciences Shenzhen Metasensing Technology Co. Ltd. 13. Zhongmin (Shenzhen) intelligent ecology Co. Ltd. Research Centre for Eco-Environmental Sciences Chinese Academy of Sciences

Wastewater-based epidemiology(WBE) has exhibited great utility in the early and rapid identification of SARS-C ***,the efficacy of wastewater surveillance under China’ s previous strict epidemic prevention policy remains to be *** collected the WBE data of wastewater treatment plants(WWTPs) in the Third People’s Hospital of Shenzhen and several communities to determine the significant effectiveness of routine wastewater surveillance in monitoring the local spread of SARS-C oV-2under tight containment of the *** results of 1 month of continuous wastewater surveillance showed that positive signals for SARS-C oV-2 RNA were detected in the wastewater samples,and a significant positive correlation was observed between the virus concentration and the number of daily *** addition,the community’ s domestic wastewater surveillance results were confirmed even 3 days before,or simultaneously with,the infected patient being confirmed as having the ***,an automated sewage virus detection robot,ShenNong No.l robot,was developed,showing a high degree of agreement with experimental data,offering the possibility of large-scale multi-point ***,our results illustrated the clear indicative role of wastewater surveillance in combating COVID-19 and provided a practical basis for rapidly expanding the feasibility and value of routine wastewater surveillance for future emerging infectious diseases.

关键词： SARS-CoV-2 wastewater surveillance wastewater-based epidemiology WWTP automated robot

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Induced pluripotent stem cell-derived motor neurons from amyotrophic lateral sclerosis(ALS)patients carrying different superoxide dismutase 1 mutations recapitulate pathological features of ALS

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Chinese Medical Journal 2021年第20期134卷 2457-2464页

作者： Wen-chao Liu Na Liu Yan Wang Chen huang Yan-Fang Li Hao Wang Xiao-Gang Li Min Deng Institute of Medical Innovation and Research Peking University Third HospitalBeijing 100191China Department of Neurology Peking University Third HospitalBeijing 100191China

Background:Investigations of the pathogenic mechanisms in motor neurons(MNs)derived from amyotrophic lateral sclerosis(ALS)disease-specific induced pluripotent stem(iPS)cell lines could improve understanding of the issues affecting ***,in this study we explored mutant superoxide dismutase 1(SOD1)protein expression in MNs derived from the iPS cell lines of ALS patients carrying different SOD1 ***:We generated induced pluripotent stem cell(iPSC)lines from two familial ALS(FALS)patients withSOD1-V14M andSOD1-C111Y mutations,and then differentiated them into *** investigated levels of the SOD1 protein in iPSCs and MNs,the intracellular Ca2+levels in MNs,and the lactate dehydrogenase(LDH)activity in the process of differentiation into the MNs derived from the controls and ALS patients’***:The iPSCs from the two FALS patients were capable of differentiation into MNs carrying different SOD1 mutations and differentially expressed MN *** detected high SOD1 protein expression and high intracellular calcium levels in both the MN and iPSCs that were derived from the twoSOD1 mutant ***,at no time did we observe stronger LDH activity in the patient lines compared with the control ***:MNs derived from patient-specific iPSC lines can recapitulate key aspects of ALS pathogenesis,providing a cell-based disease model to further elucidate disease pathogenesis and explore gene repair coupled with cell-replacement *** mutant expressions of SOD1 in MNs may have disrupted MN function,either causing or contributing to the intracellular calcium disturbances,which could lead to the occurrence and development of the disease.

关键词： Amyotrophic lateral sclerosis Induced pluripotent stem cell SOD1 gene mutation Motor neuron SOD1 aggregation

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Prognostic value of time to generalization in 71 Chinese patients with sporadic amyotrophic lateral sclerosis

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Chinese Medical Journal 2019年第9期132卷 1023-1027页

作者： Qiong-hua Sun Yan-Ran Li Wen-Jie Lan Fei Yang Fang Cui Xu-Sheng huang Department of Neurology Chinese People's Liberation Army General HospitalBeijing 100853China College of Medicine Nankai UniversityTianjin 300071China

Background:It is important to determine prognostic factors for the outcome of amyotrophic lateral sclerosis (ALS) at an early *** time taken for symptoms to spread from spinal or bulbar regions to both (time to generalization;TTG) is considered a strong predictor of survival;however,this has rarely been studied in Asian *** aim of this retrospective study was to evaluate potential factors affecting prognosis in Chinese patients with sporadic ALS,with a focus on the association between TTG and overall ***:Seventy-one patients with sporadic ALS who were hospitalized at Chinese PLA General Hospital from 2009 to 2016 were followed up until December *** analysis was performed using univariate Kaplan-Meier log-rank and multivariate Cox proportional hazards *** clinical data of the patients were recorded and *** studied were age at symptom onset,sex,site of symptom onset,diagnostic latency,TTG,diagnostic category,ALS Functional Rating Scale-revised score,percent predicted forced vital capacity (FVC%),and disease progression rate (DPR) at ***:The mean age at onset was 54 (SD = 10.2) years,and the median survival time from symptom onset was 41 months (95% confidence interval:34–47).By univariate analysis,factors independently affecting survival were age at symptom onset (Log rank = 15.652,P<0.0001),TTG (Log rank = 14.728,P<0.0001),diagnostic latency (Log rank = 11.997,P = 0.001),and DPR (Log rank = 6.50,P = 0.011).In the Cox multivariate model,TTG had the strongest impact on survival time (hazard ratio = 0.926,P = 0.01).Conclusions:TTG can be used as an effective indicator of prognosis in patients with sporadic ALS.

关键词： Amyotrophic lateral sclerosis Time to generalisation (TTG) Prognosis Survival

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Artificial intelligence model of complicated flow behaviors for Ti-13Nb-13Zr alloy and relevant applications

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Transactions of Nonferrous Metals Society of China 2019年第10期29卷 2090-2098页

作者： Ze-yan SHI Guo-zheng QUAN chao AN Hui-min QIU Wei-yong WANG Zhi-hua ZHANG CQU-UC Joint Co-op Institute Chongqing UniversityChongqing 400044China State Key Laboratory of Mechanical Transmission College of Material Science and EngineeringChongqing UniversityChongqing 400044China School of Civil Engineering Chongqing UniversityChongqing 400045China

The comprehensive nonlinear flow behaviors of a ductile alloy play a significant role in the numerical analysis of its forming process. The accurate characterization of as-forged Ti-13 Nb-13 Zr alloy was conducted by an improved intelligent algorithm, GA-SVR, the combination of genetic algorithm(GA) and support vector regression(SVR). The GA-SVR model learns from a training dataset and then is verified by a test dataset. As for the generalization ability of the solved GA-SVR model, no matter in β phase temperature range or(α+β) phase temperature range, the correlation coefficient R-values are always larger than 0.9999, and the AARE-values are always lower than 0.18%. The solved GA-SVR model accurately tracks the highly-nonlinear flow behaviors of Ti-13 Nb-13 Zr alloy. The stress-strain data expanded by this model are input into finite element solver, and the computation accuracy is improved.

关键词： Ti-13Nb-13Zr alloy flow stress constitutive model support vector regression genetic algorithm

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具连续变量的二阶中立型差分方程

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湖南师范大学自然科学学报 2005年第3期28卷 4-6页

作者：黄梅申建华湖南省第一师范学校数理系中国长沙410002 湖南师范大学数学与计算机科学学院中国长沙410081

研究一类具有连续变量的二阶中立型时滞差分方程Δ2τ[x(t)-cx(t-τ)]=p(t)x(t-σ),t≥t0>0的解的振动性,给出了其有界解振动的几个充分条件.

关键词：中立型差分方程有界解振动非振动

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非受体型蛋白酪氨酸磷酸酶12基因在脑胶质瘤中的表达及意义

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中华神经外科杂志 2021年第1期37卷 75-80页

作者：黄华王宽宇首都医科大学北京市神经外科研究所100070

目的基于中国脑胶质瘤基因组图谱计划(CGGA)和癌症基因组图谱计划(TCGA)数据库分析非受体型蛋白酪氨酸磷酸酶12基因(PTPN12)在脑胶质瘤中的表达和意义。方法回顾性分析CGGA数据库(325例)和TCGA数据库(636例)中脑胶质瘤患者的临床资料和... 详细信息

目的基于中国脑胶质瘤基因组图谱计划(CGGA)和癌症基因组图谱计划(TCGA)数据库分析非受体型蛋白酪氨酸磷酸酶12基因(PTPN12)在脑胶质瘤中的表达和意义。方法回顾性分析CGGA数据库(325例)和TCGA数据库(636例)中脑胶质瘤患者的临床资料和RNA测序数据。分析不同世界卫生组织(WHO)肿瘤级别、四分型亚型、异柠檬酸脱氢酶(IDH)突变状态、O6-甲基鸟嘌呤-DNA甲基转移酶(MGMT)启动子甲基化状态的患者PTPN12表达量的差异。根据PTPN12的表达量将患者分为PTPN12高表达组和PTPN12低表达组,采用Kaplan-Meier法绘制生存曲线,比较两组患者的生存差异。采用单因素和多因素Cox回归分析法判断PTPN12对脑胶质瘤患者的预后作用。进一步通过基因本体(GO)功能富集分析及京都基因和基因组百科全书(KEGG)通路富集分析获得基因相关的功能以及有关的通路。结果两数据库中,PTPN12的表达量均随脑胶质瘤病理级别的升高而升高(均P<0.01);与IDH突变型比较,IDH野生型中PTPN12表达量升高(均P<0.01);MGMT启动子非甲基化者PTPN12表达量高于MGMT启动子甲基化者(均P<0.01);PTPN12在经典型、间质型、神经元型以及前神经元型中表达量的差异均具有统计学意义,间质型中表达量最高(均P<0.01)。两数据库中,PTPN12高表达者均比低表达者的生存期短(均P<0.01)。两个数据库中,多因素Cox回归分析结果均显示,PTPN12表达量为脑胶质瘤患者生存期的独立影响因素(CGGA数据库中,HR=1.433,95%CI:1.094~1.876,P=0.009;TCGA数据库中,HR=1.588,95%CI:1.018~2.477,P=0.042)。GO分析结果显示,PTPN12表达量正相关的基因更多地富集在增殖、凋亡、黏附、蛋白水解、免疫反应、血管生成、药物反应、缺氧反应、肿瘤细胞G2/M期的转化、肿瘤细胞G1/S期的转化等多个与脑胶质瘤恶性进展相关的功能上。KEGG分析结果显示,与PTPN12表达量呈正相关的基因更多地富集在肿瘤相关通路、PI3K-Akt通路、丝裂原活化蛋白激酶通路、肿瘤坏死因子通路、缺氧诱导因子1通路、p53通路、凋亡通路以及细胞周期通路上。结论不同WHO肿瘤级别的脑胶质瘤患者PTPN12表达量不同,PTPN12表达量可独立用于脑胶质瘤患者的预后判断,且与多个脑胶质瘤恶性进展相关的功能和通路有关。

关键词：神经胶质瘤基因组学预后非受体型蛋白酪氨酸磷酸酶12

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二维垂直摆倾斜仪对地倾斜的响应

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哈尔滨工程大学学报 2006年第3期27卷 469-473页

作者：黄玉武立华哈尔滨工程大学理学院黑龙江哈尔滨150001

地倾斜测量不仅对地球动力学研究和地震前兆观测具有重要意义,而且在某些精密测量实验、工程建筑以及工业生产中都是不可或缺的研究内容.文中提出了一种利用二维垂直摆倾斜仪能同时测量2个方向的地倾斜信号的新方法,研究了二维垂直摆倾... 详细信息

地倾斜测量不仅对地球动力学研究和地震前兆观测具有重要意义,而且在某些精密测量实验、工程建筑以及工业生产中都是不可或缺的研究内容.文中提出了一种利用二维垂直摆倾斜仪能同时测量2个方向的地倾斜信号的新方法,研究了二维垂直摆倾斜仪对地面倾斜的响应.介绍了二维垂直摆倾斜仪的结构及工作原理,电容式微位移传感器的组成及工作原理.对二维垂直摆倾斜仪的灵敏度进行了初步标定,实验结果表明二维垂直摆倾斜仪对地倾斜信号较灵敏,已满足观测地倾斜潮汐的要求.

关键词：垂直摆倾斜仪电容式传感器地倾斜灵敏度

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Catheter ablation for atrial fibrillation is associated with reduced risk of mortality in the elderly:a prospective cohort study and propensity score analysis

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Journal of Geriatric Cardiology 2020年第12期17卷 740-749页

作者： Xin SU Xin DU Shang-Xin LU chao JIANG Jing DU Shi-Jun XIA Zhao-Jie DONG Zhao-Xu JIA De-Yong LONG Cai-hua SANG Ri-Bo TANG Nian LIU Song-Nan LI Rong BAI Jian-Zeng DONG Chang-Sheng MA Department of Cardiology Beijing Anzhen HospitalCapital Medical UniversityNational Clinical Research Centre for Cardiovascular DiseasesBeijingChina Heart Health Research Center BeijingChina The George Institute for Global Health Faculty of MedicineUniversity of New South WalesSydneyAustralia Beijing Centre for Disease Prevention and Control BeijingChina

Background It is unclear whether catheter ablation(CA)for atrial fibrillation(AF)affects the long-term prognosis in the *** study aims to evaluate the relationship between CA and long-term outcomes in elderly patients with *** Patients more than 75 years old with non-valvular AF were prospectively enrolled between August 2011 and December 2017 in the Chinese Atrial Fibrillation Registry *** who underwent CA at baseline were propensity score matched(1:1)with those who did not receive *** outcome events included all-cause mortality,cardiovascular mortality,stroke/transient ischemic attack(TIA),and cardiovascular *** Overall,this cohort included 571 ablated patients and 571 non-ablated patients with similar characteristics on 18 *** a mean follow-up of 39.75±19.98 months(minimum six months),24 patients died in the ablation group,compared with 60 deaths in the non-ablation group[hazard ratio(HR)=0.49,95%confidence interval(CI):0.30-0.79,P=0.0024].Besides,6 ablated and 29 non-ablated subjects died of cardiovascular disease(HR=0.25,95%CI:0.11-0.61,P=0.0022).A total of 27 ablated and 40 non-ablated patients suffered stroke/TIA(HR=0.79,95%CI:0.48-1.28,P=0.3431).In addition,140 ablated and 194 non-ablated participants suffered cardiovascular hospitalization(HR=0.84,95%CI:0.67-1.04,P=0.1084).Subgroup analyses according to gender,type of AF,time since onset of AF,and anticoagulants exposure in initiation did not show significant *** In elderly patients with AF,CA may be associated with a lower incidence of all-cause and cardiovascular mortality.

关键词： Atrial fibrillation Catheter ablation Mortality Stroke The elderly

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金香丹对缺血再灌注大鼠心肌细胞凋亡的影响

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中国老年学杂志 2014年第4期34卷 1014-1015页

作者：王朝阳杨化冰黄少祥湖北中医药大学基础医学院湖北武汉430065 荆州市中医医院

目的研究金香丹对缺血再灌注大鼠心肌细胞凋亡的影响。方法采用在体结扎大鼠冠状动脉左前降支建立心肌缺血再灌注模型,采用细胞凋亡测试盒(TUNEL)法观察心肌细胞凋亡情况,免疫组化法检测凋亡相关基因Bax、Bcl-2和Caspsase-3表达的变化... 详细信息

目的研究金香丹对缺血再灌注大鼠心肌细胞凋亡的影响。方法采用在体结扎大鼠冠状动脉左前降支建立心肌缺血再灌注模型,采用细胞凋亡测试盒(TUNEL)法观察心肌细胞凋亡情况,免疫组化法检测凋亡相关基因Bax、Bcl-2和Caspsase-3表达的变化。结果模型组细胞凋亡指数、Bax、Bcl-2及Caspsase-3的蛋白表达显著高于假手术组,金香丹组细胞凋亡指数、Bax及Caspsase-3的蛋白表达均显著低于模型组,Bcl-2的蛋白表达显著高于模型组。结论金香丹可以抑制缺血再灌注心肌细胞的凋亡,其作用可能与上调Bcl-2蛋白的表达同时下调Bax、Casperse-3蛋白的表达有关。

关键词：金香丹心肌缺血再灌注损伤细胞凋亡

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Palladium-Catalyzed One-Step Synthesis of Stereodefined Difunctionalized Glycals

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CCS Chemistry 2023年第3期5卷 741-749页

作者： Xiao-Ping Gong Yuke Li Hong-chao Liu Zhe Zhang Zhi-Jie Niu Ya-Nan Ding Yang An Xi Chen Yan-Chong huang Rui-Qiang Jiao Xue-Yuan Liu Yong-Min Liang State Key Laboratory of Applied Organic Chemistry Lanzhou UniversityLanzhou 730000 Department of Chemistry Centre for Scientific Modeling and ComputationChinese University of Hong KongShatinHong Kong 999077

Efficiently modifying glycals by directly introducing functional groups into their double bonds is a longstanding ***,the strategy of introducing two different functional groups into 1-iodoglycals to obtain modified glycals in one step through palladium catalysis was reported for the first time,and the modified glycals contained stereodefined tetrasubstituted *** this method,various difunctionalized glycals that were difficult to form by other routes were synthesized in moderate to good *** experiments and density functional theory calculations show that the palladium catalyst played dual roles in this transformation,namely,inducing nucleophilic substitution and catalyzing Suzuki *** reaction intermediate was isolated and confirmed by X-ray crystallographic ***,the gram-scale synthesis and facile deprotection of the target compound enhances the practicality of this strategy.

关键词： palladium catalysis glycals tetrasubstitute olefins difunctionalization stereodefined threecomponent

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