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Designing and generating a mouse model:frequently asked questions

The Journal of biomedical Research 2021年第2期35卷 76-90页

作者： channabasavaiah bgurumurthy Thomas LSaunders Masato Ohtsuka Department of Pharmacology and Experimental Neuroscience Department of Pharmacology and Experimental NeuroscienceCollege of MedicineUniversity of Nebraska Medical CenterOmahaNE 68106-5915USA Mouse Genome Engineering Core Facility Department of Pharmacology and Experimental NeuroscienceCollege of MedicineUniversity of Nebraska Medical CenterOmahaNE 68106-5915USA Department of Internal Medicine Division of Genetic MedicineUniversity of Michigan Medical SchoolAnn ArborMI 48109USA Transgenic Animal Model Core University of MichiganAnn ArborMI 48109USA Department of Molecular Life Science Division of Basic Medical Science and Molecular MedicineTokai University School of MedicineIseharaKanagawa 259-1193Japan The Institute of Medical Sciences Tokai UniversityIseharaKanagawa 259-1193Japan

Genetically engineered mouse(GEM)models are commonly used in biomedical *** GEMs involve complex set of experimental procedures requiring sophisticated equipment and highly skilled technical *** of these reasons,most research institutes set up centralized core facilities where custom GEMs are created for research ***,on the other hand,when they begin thinking about generating GEMs for their research,several questions arise in their *** example,what type of model(s)would be best useful for my research,how do I design them,what are the latest technologies and tools available for developing my model(s),and finally how to breed GEMs in my *** there are several considerations and options in mouse designs,and as it is an expensive and time-consuming endeavor,careful planning upfront can ensure the highest chance of *** this article,we provide brief answers to several frequently asked questions that arise when researchers begin thinking about generating mouse model(s)for their work.

关键词： CRISPR transgenic mouse genetic engineering knockout mouse conditional knockout mouse knock-in mouse

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A transgenic pig model expressing a CMV-ZsGreen1 reporter across an extensive array of tissues

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The Journal of biomedical Research 2021年第2期35卷 163-173页

作者： Amy T.Desaulniers Rebecca A.Cederberg Elizabeth P.Carreiro channabasavaiah b.gurumurthy brett R.White Department of Animal Science University of Nebraska-LincolnLincolnNE 68583-0908USA Department of Pharmacology and Experimental Neuroscience University of Nebraska Medical CenterOmahaNE 68198-5930USA

Since genetic engineering of pigs can benefit both biomedicine and agriculture,selecting a suitable gene promoter is critically *** cytomegalovirus(CMV)promoter,which can robustly drive ubiquitous transgene expression,is commonly used at present,yet recent reports suggest tissue-specific activity in the *** objective of this study was to quantify ZsGreen1 protein(in lieu of CMV promoter activity)in tissues from pigs harboring a CMV-ZsGreen1 transgene with a single integration *** samples(n=35)were collected from neonatal hemizygous(n=3)and homozygous(n=3)piglets and ZsGreen1 abundance was determined via ***1 was detected in all tissues,except hypothalamus,kidney cortex and *** expression patterns of homozygous and hemizygous piglets were similar(P>0.05).However,quantification revealed that ZsGreen1 protein levels were *** neural/endocrine tissues,ZsGreen1 abundance was highest in the anterior pituitary gland,intermediate in the cerebellum and lowest in the cerebrum,spinal cord and posterior pituitary(Pbundant in the salivary gland than esophagus,stomach,pancreas,duodenum,jejunum,ileum,spleen,colon,gallbladder and liver(Pbers;Pbeled organs and a valuable tool to advance genome editing research.

关键词： gene expression profiling CMV promoter ZsGreen1 biomedical model genome editing

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The effect of 2, 4-Dichlorophenoxy acetic acid and bradyrhizobium on induction of Para-nodule in wheat roots

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Journal of Agricultural Science and Technology 2009年第8期3卷 14-16页

作者： Nagaraja R. gurumurthy b. R. Devappa V Department of Microbiology College of Agriculture Navile Campus Shimoga 577204 India Areca Research Station Thirtahalli University of Horticultural Sciences Bagalkot 587301 India Department of Plant Pathology College of Agriculture Navile Campus Shimoga 577204 India

The investigation was conducted to know the effect of 2, 4-Dichlorophenoxy acetic acid and inoculation on Para-nodule formation and growth parameters in wheat. Formation of nodular outgrowth on the roots of wheat seedlings treated with 2, 4-D commenced in 8-10 days. The maximum Para-nodule formation was found in 1 ppm of 2, 4-D when incubated with bradyrhizobium P-132. Inoculation of the same has helped to increase the number of Para-nodule, but not essential for Para-nodulation. Histological study shows that, these induced Para-nodules originated from the pericycle and these are appeared to be modified lateral roots and Para-nodule structure formations however, it enhanced by bradyrhizobial inoculation.

关键词： Para-nodule 2, 4-D pericycle N-fixation

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