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A nitroreductase-responsive nanoprobe with homogeneous composition and high loading for preoperative non-invasive tumor imaging and intraoperative guidance

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Chinese Chemical Letters 2024年第7期35卷 313-318页

作者： Jingqi Xin Shupeng Han Meichen Zheng Chenfeng Xu Zhongxi Huang Bin Wang changmin yu Feifei An yu Ren Institute of Medical Engineering Department of BiophysicsSchool of Basic Medical ScienceHealth Science CenterXi'an Jiaotong UniversityXi'an 710061China School of Public Health Health Science CenterXi'an Jiaotong UniversityXi'an 710061China Key Laboratory of Flexible Electronics(KLOFE)&Institute of Advanced Materials(IAM) Nanjing Tech UniversityNanjing 211816China Department of Breast Surgery the First Affiliated Hospital of Xi'an Jiaotong UniversityXi'an 710061China

Tumor microenvironment(TME)-activatable probes have been proven to effectively increase signal-tobackground ratios(SBRs)and improve the success rate of complete tumor ***,many fluorescence probes have to be loaded into a nanocarrier for tumor targeted delivery,which consequently encounters poor drug loading,heterogeneous composition and non-encapsulated drug aggregates occurred during nanoformulation ***,a nitroreductase(NTR)-activated“OFF-ON”near-infrared fluorescence nanoprobe,named Nano Bodipy,was synthesized by the spontaneous self-assembling of NTRresponsive dye-polyethylene glycol(PEG)amphiphilic polymer in *** NTR-responsive dye acted as the hydrophobic segment in the amphiphilic polymer,yielding a homogeneous composition and a high loading of 12.2 wt%(according to calculation)in the synthesized Nano *** synthesized Nano Bodipy can efficiently accumulate in tumors via the enhanced permeability and retention(EPR)effect,enabling non-invasive tumor-targeted fluorescence imaging and guiding complete tumor *** the synthesized Nano Bodipy entered the tumor cells,they dissociated and were activated by overexpressed *** the real-time fluorescence guide of Nano Bodipy,complete tumor resection surgery was performed successfully.

关键词： Nitroreductase Tumor diagnosis Intraoperative guidance Self-assembly Nanoprobe

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磁分离技术和纳米金比色法用于嗜碱性粒细胞活化试验研究

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材料导报 2024年第9期38卷 249-255页

作者：王迎迎刘永欣沈倩付婵余昌敏东南大学附属中大医院南京210009 南京工业大学柔性电子(未来技术)学院南京210009

嗜碱性粒细胞活化试验是一种安全、高效的诊断过敏疾病技术,成为临床体外诊断过敏疾病的主要方法之一。目前,嗜碱性粒细胞活化试验主要是利用流式细胞术对细胞进行检测,这种方法通常存在检测时间长、操作繁琐和成本高等缺点。针对这些问... 详细信息

嗜碱性粒细胞活化试验是一种安全、高效的诊断过敏疾病技术,成为临床体外诊断过敏疾病的主要方法之一。目前,嗜碱性粒细胞活化试验主要是利用流式细胞术对细胞进行检测,这种方法通常存在检测时间长、操作繁琐和成本高等缺点。针对这些问题,本研究开发了一种表面修饰HLA-DR抗体的磁珠分离体系,将其与过敏患者的外周血共孵育5 min后,利用磁性分离技术,筛选出表面含有HLA-DR标记物的细胞。测试结果表明,筛选后的嗜碱性粒细胞在全血中的细胞占比从0.2%提升到11.5%,嗜碱性粒细胞活化试验检测灵敏性得到了有效提高。进一步地,本研究构建了CD63适配体修饰的金纳米粒子检测体系,利用CD63适配体特异性识别活化后的嗜碱性粒细胞表面的CD63抗体,使金纳米粒子在嗜碱性粒细胞表面聚集,溶液颜色由红色转变为蓝紫色,实现对激活的嗜碱性粒细胞可视化比色检测,为临床开展嗜碱性粒细胞活化试验提供了新的方法。

关键词：比色法金纳米粒子嗜碱性粒细胞激活实验磁分离适配体

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金纳米粒子的表面修饰及生物应用进展

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化学通报 2024年第6期87卷 642-651页

作者：赵陈泷沈倩黄众熙陈泽娜杨乃娣余昌敏南京工业大学柔性电子(未来技术)学院南京210009

近年来纳米材料被广泛应用于生物医学、航空航天和精细化工等领域。构成纳米材料的纳米粒子具有小尺寸效应、表面效应和宏观量子隧道效应等性质。其中金纳米粒子(AuNPs)由于其独特的荧光特性、良好的生物相容性和表面等离子共振等性质,... 详细信息

近年来纳米材料被广泛应用于生物医学、航空航天和精细化工等领域。构成纳米材料的纳米粒子具有小尺寸效应、表面效应和宏观量子隧道效应等性质。其中金纳米粒子(AuNPs)由于其独特的荧光特性、良好的生物相容性和表面等离子共振等性质,被广大科研人员深入研究。然而,目前AuNPs仍存在水分散性差、肾清除效率低和量子发射产率低等问题,限制了其广泛应用。因此,研究者对AuNPs表面进行多样化修饰,从而能有效克服上述缺点。本文就目前主流配体表面修饰AuNPs的研究进展进行总结,着重介绍了功能化AuNPs在生物成像、生物检测、生物治疗三方面的应用,最后对AuNPs的临床治疗机制的探索以及商业化的应用进行了展望,希望能为相关领域的研究者们提供新思路。

关键词：金纳米粒子表面修饰生物应用

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Dimorphism in Calceola sandalina (Linneé, 1771)

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Science China Earth Sciences 2007年第12期50卷 1761-1766页

作者： yu changmin Nanjing Institute of Geology and Palaeontology Chinese Academy of Sciences NIGPAS Nanjing 210008 China

Based on studying the ontogenetic development of Calceola sandalina in the Early Devonian yukiang Formation from Guangxi Zhuang Autonomous Region, South China, it is for the first time to issue the evidence indicating the existence of sexual dimorphism in Calceola sandalina. The new discovery will add important data on sexual dimorphism throughout the non-colonial fossil metazoan, and it also may throw lights on studying the taxonomy and phylogeny of Calceola sandalina and its relevant taxa.

关键词： biocoenosis, benthonics, sexual dimorphism, bimodal variation, metazoans, ecomorphs, brood pouches

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Rational design of nanocarriers for mitochondria-targeted drug delivery

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Chinese Chemical Letters 2022年第9期33卷 4146-4156页

作者： Lihua Huang Zonghao Sun Qian Shen Zhongxi Huang Shuangxi Wang Naidi Yang Gongqiang Li Qiong Wu Wei Wang Lin Li changmin yu Key Laboratory of Flexible Electronics(KLOFE)&Institute of Advanced Materials(IAM) Nanjing Tech UniversityNanjing 211816China Institute of Agro-product Safety and Nutrition Zhejiang Academy of Agricultural SciencesHangzhou 310021China State Key Laboratory of Coordination Chemistry Nanjing UniversityNanjing 210023China

Well-developed mitochondria-targeted nanocarriers for function regulation are highly *** studies have been conducted on the treatment of mitochondria-related diseases;however,further improvements are required to develop more effective drug delivery ***,we comprehensively introduce recent developments progress in rational design of mitochondria-targeted nanocarriers,and discuss the different strategies of available nanocarriers for targeting *** also highlight the advantages and disadvantages of various carrier systems that are currently in ***,perspective on new generation for mitochondria-targeted delivery systems in the emerging area of drug-based therapeutics is also discussed.

关键词： Mitochondria target Mitochondria diseases Therapeutic drugs Nanocarriers

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Ultrasensitive detection of IgE levels based on magnetic nanocapturer linked immunosensor assay for early diagnosis of cancer

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Chinese Chemical Letters 2022年第4期33卷 1855-1860页

作者： Yongxin Liu Mengda Cao Zhongxi Huang changmin yu Naidi Yang Qiong Wu Liang Shi Wenjie Duan Yi Zhu Jifu Wei Lin Li Wei Huang Key Laboratory of Flexible Electronics(KLOFE)&Institute of Advanced Materials(IAM) Nanjing Tech University(NanjingTech)Nanjing 211816China Research Division of Clinical Pharmacology&Clinical Allergy Center the First Affiliated Hospital of Nanjing Medical UniversityDepartment of PharmacyJiangsu Institute of Cancer Research&the Affiliated Cancer Hospital of Nanjing Medical UniversityNanjing 210009China Department of Pharmacy Zhongda HospitalSchool of MedicineSoutheast UniversityNanjing 210044China State Key Laboratory of Coordination Chemistry Nanjing UniversityNanjing 210023China Shaanxi Institute of Flexible Electronics(SIFE) Northwestern Polytechnical University(NPU)Xi’an 710072China The First Affiliated Hospital of USTC Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001China

Rapid and accurate detection of immunoglobulin E(Ig E) in serum and reduction of serum dosage are of great significance for clinical detection. Herein, we described a rapid magnetic separation of Ig E from patient serum based on Fe3 O4@Si O2-NTA@026 sdab as the capture probe and multiple horseradish peroxidase(HRP)-labeled antibodies linked gold nanoparticles(Au NPs) as chemiluminescence(CL) signal amplifier for ultrasensitive detection of total Ig E. Results showed that the limit of detection of our immunosensor system in serum samples was 0.03 k U/L, which is lowest in comparison with current methods, and far lower than that of Immuno CAP for Ig E detection(0.1 k U/L). Furthermore, our immunosensor possessed satisfied repeatability and accuracy, as well as good stability. In comparison with the Immuno CAP for the quantitative detection of Ig E, highly consistent results were achieved in 20 serum samples. Specially, this method was also successfully utilized for assessing the Ig E traces in breast cancer patients,which provides a new idea for the diagnosis of early cancer. Therefore, we believe that such versatile immunosensor will offer an alternative method for the on-site monitoring and determination of various Ig E-related diseases.

关键词： Gold nanoparticles Magnetic nanocapturer IgE detection Ultrasensitive immunosensor Cancer

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New Data on the Age of the yukiang (yujiang) Formation at Liujing, Guangxi, South China

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Acta Geologica Sinica(English Edition) 2017年第4期91卷 1438-1447页

作者： LU Jianfeng QIE Wenku yu changmin CHEN Xiuqin Nanjing Institute of Geology andPalaeontology Chinese Academy of Sciences Nanjing 210008 China Key Laboratory of Economic Stratigraphy and Palaeogeography Nanjing Institute of Geology and Palaeontology Chinese Academy of Sciences Nanjing 210008 China

The age of the yukiang （yujiang） Formation has been shrouded in controversy for more than 75 years. New investigation at the Liujing section, Hengxian County, Guangxi, has revealed a conodont sequence assignable to two Early Devonian conodont zonations： the Eognathodus kindlei？-Polygnathus pireneae Zone and the Polygnathus nothoperbonus Zone from oldest to youngest. The Gaoling and Mahuangling members of the Nahkaoling （Nagaoling） Formation and the overlying Xiayiling Member and lower part of the Shizhou Member of the yukiang Formation are proven to belong to the Pragian Eognathodus kindlei？-Polygnathus pireneae Zone. The co-occurrence of Polygnathus pireneae and Pol. sokolovi in the lower part of the Shizhou Member demonstrates that this interval can be approximately correlated with the uppermost part of the Polygnathus pireneae Zone. However, precise level identification of the Pragian-Emsian Boundary （PEB） in the Liujing section remains dfficult due to the scarcity of suitable limestone samples for conodont analysis from the middle and upper parts of the Shizhou Member. The Daliancun and Liujing members of the yukiang Formation and the lower part of the overlying Moding Formation are assigned to the Polygnathus nothoperbonus Zone with the nominal species of this biozone ranging from the bottom of the Daliancun Member to the lower part of the Moding Formation.

关键词： conodonts Nahkaoling Formation yukiang Formation Liujing section Pragian Emsian

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A Comprehensive Evaluation on the Devonian-Carboniferous Boundary Stratotype Candidate Section in Nanbiancun, Guilin, Guangxi

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Acta Geologica Sinica(English Edition) 1990年第1期64卷 69-79页

作者： yu changmin Nanjing Institute of Geology and Palaeontology, Academia Sinica 2This paper is extracted from some relevant chapters of a monograph. The authors who wrote the monograph are: yu changmin Ruan Yiping Xu Hankui Wang Shangqi Zhu Zhaoling Mu Xinan Wang Keliang Yin Bao' an Li Zhengliang Li Yukuan Guo Shuying Yao Zhaogui He Guiying Zhang Yue'e Lu Hongjin Huang Zhicheng Zhu Sizhao Yang Jiedong Wang Yinxi Ye Sujuan and Xu Tongchun.

This paper presents a comprehensive evaluation on the Devonian-Carboniferous Boundary (DCB) sectionin Nanbiancun. Guilin. Guangxi on the basis of Palaeontological, biostratigraphical and sedimentarypetrological studies as well as microfacies analysis. REE and trace element analysis and isotopic dating. The DCB at this section conforms to the definition of this boundary and essentially measure up the criteriaand conditions for selection of the boundary stratotype. Up to 14 fossil groups are contained in the section. Thesection presents a general biostratigraphical framework with multispecies fossils zones, assemblage zones andmultiple groups of fossils and has latent preponderant conditions for magnetostratigraphic andchronostratigraphic studies. The boundary and point occur in a persistent and continuous monofacialcarbonate succession. and the section is located in a tourist city with convenient communication, where variousfacies of DCB sections are developed. allowing the correlation within a small area. So it is an ideal candidateboundary stratotype.

关键词： Guilin Guangxi A Comprehensive Evaluation on the Devonian-Carboniferous Boundary Stratotype Candidate Section in Nanbiancun

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主体的电影哲学:卡维尔、怀疑主义与媒介特殊性

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电影艺术 2022年第1期 12-18页

作者：于昌民美国爱荷华大学

本文从电影哲学的基本假设与路径出发,透过德勒兹的框架分析电影哲学对于真假问题的态度、电影与世界的关系、媒介特殊性和历史演变等核心议题。透过分析《理性的要求》中的日常语言哲学、怀疑主义来演绎《看见的世界——关于电影本体... 详细信息

本文从电影哲学的基本假设与路径出发,透过德勒兹的框架分析电影哲学对于真假问题的态度、电影与世界的关系、媒介特殊性和历史演变等核心议题。透过分析《理性的要求》中的日常语言哲学、怀疑主义来演绎《看见的世界——关于电影本体论的思考》中的电影本体论以及概念上的关联性。这些分析将有助于我们厘清电影的媒介特殊性和主体与现代世界疏离之间的关系,并帮助理解为什么美国哲学家卡维尔的电影哲学可以继承德勒兹在英美学界的地位。

关键词：电影哲学德勒兹卡维尔怀疑主义媒介特殊性

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Recent progress in small molecule fluorescent probes for nitroreductase

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Chinese Chemical Letters 2018年第10期29卷 1451-1455页

作者： Wenjing Qin Chenchen Xu Yanfei Zhao changmin yu Sheng Shen Lin Li Wei Huang Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM) Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM)Nanjing Tech University (NanjingTech)Nanjing 211816 China Department of Sports Medicine and Adult Reconstructive Surgery Drum Tower HospitalSchool of MedicineNanjing UniversityNanjing 210008 China

Nitroreductase（NTR） is a member of flavin-containing enzymes that exists widely in bacteria. Hypoxia,which is a characteristic of locally advanced solid tumors, resulting from an imbalance between oxygen consumption and supply, can result in NTR overexpression. Using either nicotinamide adenine dinucleotide（NADH） or nicotinamide adenine dinucleotide phosphate（NADPH） as a source of reducing equivalents, NTR can catalyze the reduction of nitroaromatic compounds to the corresponding *** on this reduction mechanism, NTR can be applied not only in the bioremediation and degradation of organic nitrogen compounds, but also in the development of NTR-targeted fluorescent probes to detect the hypoxic status of cancer cells. This review aims to provide a summary of the progress in fluorescent probes for NTR in recent years and elucidate the main fluorescent mechanisms that have been applied to design probes.

关键词： Probe Fluorescence Nitroreductase Small molecule probes

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