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Identification and characterization of genes related to m^(6)A modification in kiwifruit using RNA-seq and ATAC-seq

Horticultural Plant Journal 2024年第5期10卷 1121-1133页

作者： Xiaoli Hu Tong Li changbin xu Yanna xu Congjun You Xinyi Li Jinli Gong Xiaolong Li xuepeng Sun Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province Key Laboratory of Quality and Safety Control for Subtropical Fruit and VegetableMinistry of Agriculture and Rural AffairsCollege of Horticulture ScienceZhejiang A&F UniversityHangzhouZhejiang 311300China

N6-methyladenosine(m^(6)A)RNA modification is a conserved mechanism that regulates the fate of RNA across eukaryotic *** its significance,a comprehensive analysis of m^(6)A-related genes in non-model plants,such as kiwifruit,is ***,we identified 36 m^(6)A-related genes in the kiwifruit genome according to homology and phylogenetic *** performed bioinformatics and evolutionary analyses of the writer,eraser,and reader families of m^(6)A *** of public RNA-seq data collected from samples under various biotic and abiotic stresses indicated that most m^(6)A-related genes were remarkably expressed under different *** construction of gene co-expression networks,we found significant correlations between several m^(6)A-related genes and transcription factors(TFs)as well as receptor-like genes during the development and ripening of ***,we performed ATAC-seq assays on diverse kiwifruit tissues to investigate the regulatory mechanisms of m^(6)A-related *** identified 10 common open chromatin regions that were present in at least two tissues,and these regions might serve as potential binding sites for MADS protein,C2H2 protein,and other predicted *** study offers comprehensive insights into the gene family of m^(6)A-related components in kiwifruit,which will lay foundation for exploring mechanisms of post-transcriptional regulation involved in development and adaptation of kiwifruit.

关键词： Kiwifruit N^(6)-methyladenosine Phylogenetic analysis cis-element Co-expression network

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基于共生理论构建全民国防教育体系的路径研究

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广东交通职业技术学院学报 2024年第4期23卷 116-120页

作者：许娟许昌斌海南职业技术学院海南海口570216

在实现中国梦和强军梦的伟大征程中,全民国防教育是关乎国家安全和国防建设的重要战略问题。本文基于共生理论,通过分析全民国防教育共生单元、共生模式和共生环境的结构关系,探索共生理论嵌入全民国防教育体系的实践模式。从“政社军... 详细信息

在实现中国梦和强军梦的伟大征程中,全民国防教育是关乎国家安全和国防建设的重要战略问题。本文基于共生理论,通过分析全民国防教育共生单元、共生模式和共生环境的结构关系,探索共生理论嵌入全民国防教育体系的实践模式。从“政社军校家”协同,融合全民国防教育共生单元;共建共享共创,构建互惠共赢全民国防教育共生模式;全方位覆盖,营造正向激励的全民国防教育共生环境等维度提出了构建全民国防教育体系的路径选择,以期优化全民国防教育一体化共生模式,实现全民国防教育高质量发展。

关键词：共生理论全民国防教育体系构建

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Vulnerability assessment of UAV engine to laser based on improved shotline method

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Defence Technology（防务技术） 2024年第3期33卷 588-600页

作者： Le Liu Chengyang xu changbin Zheng Sheng Cai Chunrui Wang Jin Guo Changchun Institute of Optics Fine Mechanics and PhysicsChinese Academy of SciencesChangchun 130033China University of Chinese Academy of Sciences Beijing 100049China

Laser anti-drone technology is entering the sequence of actual combat,and it is necessary to consider the vulnerability of typical functional parts of *** the concept of"vulnerability"was proposed,a variety of analysis programs for battlefield targets to traditional weapons have been developed,but a comprehensive assessment methodology for targets'vulnerability to laser is still *** on the shotline method,this paper proposes a method that equates laser beam to shotline array,an efficient vulnerability analysis program of target to laser is established by this method,and the program includes the circuit board and the wire into the vulnerability analysis category,which improves the precision of the vulnerability *** the UAV engine part as the target of vulnerability analysis,combine with the"life-death unit method"to calculate the laser penetration rate of various materials of the UAV,and the influence of laser weapon system parameters and striking orientation on the killing probability is quantified after introducing the penetration rate into the vulnerability analysis *** quantitative analysis method proposed in this paper has certain general expansibility,which can provide a fresh idea for the vulnerability analysis of other targets to laser.

关键词： Laser weapon Laser damage Vulnerability UAV Engine Killing probability

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Investigation of Terraces along the Luoyunshan Piedmont Fault Zone and Its Tectonic Implications

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Earthquake Research in China 2012年第2期26卷 210-221页

作者： Sun changbin Xie Xinsheng xu Jianhong Laboratory of Crustal Dynamics Institute of Crustal Dynamics CEA Beijing 100085 China

Located at the west of the Linfen basin, the Luoyunshan piedmont fault zone controls the western boundary of the basin. According to the measurements of the terraces in eight gullies along the Luoyunshan fault zone, five levels of terraces, namely T1～T5 have developed in these gullies. The heights of terraces T1, T2, T3, T4 and T5 are about 3m, 8～10m, about 20m, about 30m and 40～50m, respectively. The dating data of the terraces and investigation of the faulted landforms show that the Luoyunshan fault zone has experienced much activity since the Late Quaternary. The uplift rate of the terraces was 0.41mm/a since the Middle-Late Pleistocene, and 0.75mm/a since the Holocene. The increasing trend of uplift rate of the terraces along the Luoyunshan fault zone from the Middle-Late Pleistocene to Holocene indicates the tendency of gradual tectonic uplift of the fault zone since the late Quaternary. This is in good agreement with the increasing trend of subsidence rate of the Linfen basin from the Late Pleistocene to Holocene.

关键词： Luoyunshan piedmont fault zone Terrace Late Quaternary Uplift rate Tectonic implication

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我国证人宣誓制度存在与发展研究

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前沿 2010年第20期 108-111页

作者：徐长斌江西师范大学鹰潭学院江西鹰潭335000

西方国家证人制度的建立和完善在很大程度上得益于证人宣誓制度的存在和发展。而我国理论界在研究证人出庭问题或证人制度时却较少涉及证人宣誓制度,研究证人宣誓制度时又较少涉及证人出庭问题。造成研究过程中脱节现象是对证人宣誓制... 详细信息

西方国家证人制度的建立和完善在很大程度上得益于证人宣誓制度的存在和发展。而我国理论界在研究证人出庭问题或证人制度时却较少涉及证人宣誓制度,研究证人宣誓制度时又较少涉及证人出庭问题。造成研究过程中脱节现象是对证人宣誓制度在整个证人制度中的地位和作用忽视的结果,表明了我国证人宣誓制度研究视阈的褊狭。因此必须确立证人宣誓制度研究的新理路,以缓解我国证人出庭难问题和进一步推进司法改革的步伐。

关键词：证人宣誓证人保证书司法改革

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Scientific objectives and payload configuration of the Chang'E-7 mission

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National Science Review 2024年第2期11卷 285-300页

作者： Chi Wang Yingzhuo Jia changbin xue Yangting Lin Jianzhong Liu Xiaohui Fu Lin xu Yun Huang Yufen Zhao Yigang xu Rui Gao Yong Wei Yuhua Tang Dengyun Yu Yongliao Zou State Key Laboratory of Space Weather National Space Science Center Chinese Academy of Sciences National Space Science Center Chinese Academy of Sciences Institute of Geology and Geophysics Chinese Academy of Sciences Institute of Geochemistry Chinese Academy of Sciences Shandong Provincial Key Laboratory of Optical Astronomy and Solar-Terrestrial Environment School of Space Science and Physics Institute of Space SciencesShandong University Institute of Drug Discovery Technology Ningbo University State Key Laboratory of Isotope Geochemistry Guangzhou Institute of Geochemistry Chinese Academy of Sciences School of Earth Science and Engineering Sun Yat-sen University Lunar Exploration and Space Engineering Center China Aerospace Science and Technology Corporation

As the cornerstone mission of the fourth phase of the Chinese Lunar Exploration Program, Chang'E-7(CE-7) was officially approved, and implementation started in 2022, including a main probe and a communication relay satellite. The main probe, consisting of an orbiter, a lander, a rover and a mini-flying probe, is scheduled to be launched in 2026. The lander will land on Shackleton crater's illuminated rim near the lunar south pole, along with the rover and mini-flying probe. The relay satellite(named Queqiao-2) will be launched in February 2024 as an independent mission to support relay communication during scientific exploration undertaken by Chang'E-4, the upcoming Chang'E-6 in 2024 and subsequent lunar *** CE-7 mission is mainly aimed at scientific and resource exploration of the lunar south pole. We present CE-7's scientific objectives, the scientific payloads configuration and the main functions for each scientific payload with its key technical specifications.

关键词： Chang’ E-7 scientific objectives scientific payloads

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The Clausena lansium genome provides new insights into alkaloid diversity and the evolution of the methyltransferase family

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Journal of Integrative Agriculture 2024年第10期23卷 3537-3553页

作者： Yongzan Wei Yi Wang Fuchu Hu Wei Wang changbin Wei Bingqiang xu Liqin Liu Huayang Li Can Wang Hongna Zhang Zhenchang Liang Jianghui Xie State Key Laboratory of Biological Breeding of Tropical Crops Institute of Tropical Bioscience and BiotechnologyChineseAcademy of Tropical Agricultural SciencesHaikou 571101China Key Laboratory of Genetic Resources Evaluation and Utilization of Tropical Fruits and Vegetables(Co-construction by Ministryand Province) Ministry of Agriculture and Rural Affairs/Institute of Tropical Fruit TreesHainan Academy of Agricultural SciencesHaikou 571100China Beijing Key Laboratory of Grape Sciences and Enology CAS Key Laboratory of Plant ResourcesInstitute of BotanyChineseAcademy of SciencesBeijing 100093China Key Laboratory of Tropical Fruit Biology(Ministry of Agriculture and Rural Affairs) South Subtropical Crops Research InstituteChinese Academy of Tropical Agricultural SciencesZhanjiang 524091China Haikou Experimental Station Chinese Academy of Tropical Agricultural SciencesHaikou 571101China Sanya Institute of Breeding and Multiplication/School of Tropical Agriculture and Forestry Hainan UniversityHaikou 570228China Sanya Research Academy Chinese Academy of Tropical Agricultural SciencesSanya 572019China

Wampee(Clausena lansium)is an important evergreen fruit tree native to southern China that has a long history of use for medicinal ***,a chromosome-level genome of *** was constructed with a genome size of 282.9 Mb and scaffold N50 of 30.75 *** assembled genome contains 48.70%repetitive elements and 24,381 protein-coding *** genomic analysis showed that *** diverged from Aurantioideae 15.91-24.95 million years ***,some expansive and specific gene families related to methyltransferase activity and S-adenosylmethionine-dependent methyltransferase activity were also *** analysis indicated that N-methyltransferase(NMT)is mainly involved in alkaloid biosynthesis and O-methyltransferase(OMT)participates in the regulation of coumarin accumulation in *** suggested that wampee's richness in alkaloids and coumarins might be due to the gene expansions of NMT and *** tandem repeat event was one of the major reasons for the NMT ***,the reference genome of *** will facilitate the identification of some useful medicinal compounds from wampee resources and reveal their biosynthetic pathways.

关键词： Clausena lansium genome evolution methyltransferase activity alkaloid biosynthesis coumarin accumulation

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Clinical and molecular significance of homologous recombination deficiency positive non-small cell lung cancer in Chinese population:An integrated genomic and transcriptional analysis

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Chinese Journal of Cancer Research 2024年第3期36卷 282-297页

作者： Yifei Wang Yidan Ma Lei He Jun Du Xiaoguang Li Peng Jiao Xiaonan Wu Xiaomao xu Wei Zhou Li Yang Jing Di changbin Zhu Liming xu Tianlin Sun Lin Li Dongge Liu Zheng Wang Department of Pathology Department of Minimally Invasive Tumor Therapies Center Department of Thoracic Surgery Department of Oncology Department of Respiratory and Critical Care Medicine Beijing HospitalNational Center of GerontologyInstitute of Geriatric MedicineChinese Academy of Medical SciencesBeijing 100730China Amoy Diagnostics Co. Ltd.Xiamen 361027China

Objective:The clinical significance of homologous recombination deficiency(HRD)in breast cancer,ovarian cancer,and prostate cancer has been established,but the value of HRD in non-small cell lung cancer(NSCLC)has not been fully *** study aimed to systematically analyze the HRD status of untreated NSCLC and its relationship with patient prognosis to further guide clinical ***:A total of 355 treatment-naïve NSCLC patients were retrospectively *** status was assessed using the AmoyDx Genomic Scar Score(GSS),with a score of≥50 considered ***,transcriptomic,tumor microenvironmental characteristics and prognosis between HRD-positive and HRDnegative patients were ***:Of the patients,25.1%(89/355)were *** to HRD-negative patients,HRDpositive patients had more somatic pathogenic homologous recombination repair(HRR)mutations,higher tumor mutation burden(TMB)(P<0.001),and fewer driver gene mutations(P<0.001).Furthermore,HRD-positive NSCLC had more amplifications in PI3K pathway and cell cycle genes,MET and MYC in epidermal growth factor receptor(EGFR)/anaplastic lymphoma kinase(ALK)mutant NSCLC,and more PIK3CA and AURKA in EGFR/ALK wild-type ***-positive NSCLC displayed higher tumor proliferation and immunosuppression ***-negative NSCLC showed activated signatures of major histocompatibility complex(MHC)-II,interferon(IFN)-γand effector memory CD8+T ***-positive patients had a worse prognosis and shorter progressionfree survival(PFS)to targeted therapy(first-and third-generation EGFR-TKIs)(P=0.042).Additionally,HRDpositive,EGFR/ALK wild-type patients showed a numerically lower response to platinum-free immunotherapy ***:Unique genomic and transcriptional characteristics were found in HRD-positive *** prognosis and poor response to EGFR-TKIs and immunotherapy were observed in HRD-positive *** study highlights potential actionable alterations in HRD

关键词： Non-small cell lung cancer homologous recombination deficiency genetic alterations transcriptional analysis tumor microenvironment prognosis

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我国刑事证人不出庭作证行为的理性分析

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井冈山大学学报（社会科学版） 2009年第3期30卷 80-83页

作者：徐长斌江西师范大学鹰潭学院

证人出庭问题一直困扰着我国司法改革和实践,时至今日,证人出庭难的困境依然未得到有效缓解。究其原因,主要是因为对证人不出庭行为本身缺乏必要的分析,没有认识到证人不出庭行为存在的合理性和价值性,从而对证人出庭行为认识不全面,导... 详细信息

证人出庭问题一直困扰着我国司法改革和实践,时至今日,证人出庭难的困境依然未得到有效缓解。究其原因,主要是因为对证人不出庭行为本身缺乏必要的分析,没有认识到证人不出庭行为存在的合理性和价值性,从而对证人出庭行为认识不全面,导致解决措施实效性降低。而通过对证人不出庭行为的合理性研究,能够促成研究视角的转换、提高全面思考的意识和增强措施的实效性。

关键词：证人不出庭作证合理性

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Genetic basis of geographical differentiation,breeding selection,domestication effects,and breeding application for TaJAZ1 in wheat

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Journal of Genetics and Genomics 2024年第6期51卷 665-668页

作者： Botao Ye Yangyang Liu Ziying Wang Liping Shen changbin Yin Kuocheng Shen Jiaqiang Sun Xiaowan xu Mengjing Sun Jianhui Wu Zhonghu He xuchang Yu Fei Lu Yuanfeng Hao Zifeng Guo Key Laboratory of Plant Molecular Physiology Institute of BotanyChinese Academy of SciencesBeijing 100093China University of Chinese Academy of Sciences Beijing 100049China China National Botanical Garden Beijing 100093China State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyInnovative Academy of Seed DesignChinese Academy of SciencesBeijing 100101China Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China State Key Laboratory of Crop Stress Biology for Arid Areas Northwest A&F UniversityYanglingShaanxi 712100China International Maize and Wheat Improvement Center China Office c/o CAASBeijing 100081China CAS-JIC Centre of Excellence for Plant and Microbial Science Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China

Common wheat(Triticum aestivum L.,2n=6x=42,AABBDD)is one of the most widely grown crops worldwide,providing about 20%of the daily calories and protein consumed by humans(Shiferaw et al.,2013).The world population is projected to reach 9.8 billion in 2050(https://***/en/desa/world-population-projectedreach-98-billion-2050-and-112-billion-2100);thus,to meet the challenges of global food and nutritional security,wheat yields must continue to be increased through breeding programs and improved agricultural techniques(Hunter et al.,2017).

关键词： breeding crops wheat

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