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Multiple factors to assist human-derived induced pluripotent stem cells to efficiently differentiate into midbrain dopaminergic neurons

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Neural Regeneration Research 2024年第4期19卷 908-914页

作者： Yalan Chen Junxin kuang Yimei Niu Hongyao Zhu Xiaoxia Chen Kwok-Fai So Anding Xu Lingling Shi Guangdong-Hong Kong-Macao Institute of CNS Regeneration Ministry of Education CNS Regeneration Collaborative Joint LaboratoryJinan University Department of Neurology and Stroke Center The First Affiliated HospitalJinan University Department of Psychiatry The First Affiliated HospitalJinan University Co-innovation Center of Neuroregeneration Nantong University

Midbrain dopaminergic neurons play an important role in the etiology of neurodevelopmental and neurodegenerative *** also represent a potential source of transplanted cells for therapeutic *** vitro differentiation of functional midbrain dopaminergic neurons provides an accessible platform to study midbrain neuronal dysfunction and can be used to examine obstacles to dopaminergic neuronal *** evidence and impressive advances in human induced pluripotent stem cells,with tuned neural induction and differentiation protocols,makes the production of induced pluripotent stem cell-derived dopaminergic neurons *** SB431542 and dorsomorphin dual inhibitor in an induced pluripotent stem cell-derived neural induction protocol,we obtained multiple subtypes of neurons,including 20%tyrosine hydroxylase-positive dopaminergic *** obtain more dopaminergic neurons,we next added sonic hedgehog(SHH)and fibroblast growth factor 8(FGF8)on day 8 of *** increased the proportion of dopaminergic neurons,up to 75%tyrosine hydroxylase-positive neurons,with 15%tyrosine hydroxylase and forkhead box protein A2(FOXA2)co-expressing *** further optimized the induction protocol by applying the small molecule inhibitor,CHIR99021(CHIR).This helped facilitate the generation of midbrain dopaminergic neurons,and we obtained 31-74%midbrain dopaminergic neurons based on tyrosine hydroxylase and FOXA2 ***,we have established three induction protocols for dopaminergic *** on tyrosine hydroxylase and FOXA2 immunostaining analysis,the CHIR,SHH,and FGF8 combined protocol produces a much higher proportion of midbrain dopaminergic neurons,which could be an ideal resource for tackling midbrain-related diseases.

关键词： dopaminergic neurons FGF signal induced pluripotent stem cells midbrain neural differentiation SHH signal SMAD signal WNT signal

Inhibition of SLC26A4 regulated by electroacupuncture suppresses the progression of myocardial ischemia-reperfusion injury

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BIOCELL 2024年第4期48卷 665-675页

作者： FEI KONG QIYUAN TIAN BINGLIN kuang LILI SHANG XIAOXIAO ZHANG DONGYANG LI YING KONG Department of Endocrinology Heilongjiang University of Chinese MedicineHarbin150040China Department of Cardiology Heilongjiang University of Chinese MedicineHarbin150040China College of Clinical Medicine Heilongjiang University of Chinese MedicineHarbin150040China Department of Nephrology Beijing Hospital of Integrated Traditional Chinese and Western MedicineBeijing100039China College of Acupuncture and Massage Heilongjiang University of Chinese MedicineHarbin150040China Department of Acupuncture Heilongjiang University of Chinese MedicineHarbin150040China

Introduction:Myocardial ischemia-reperfusion(IR)injury has received widespread attention due to its damaging ***(EA)pretreatment has preventive effects on myocardial IR ***26A4 is a Na+independent anion reverse transporter and has not been reported in myocardial IR ***:Toﬁnd potential genes that may be regulated by EA and explore the role of this gene in myocardial IR ***:RNA sequencing and bioinformatics analysis were performed to obtain the differentially expressed genes in the myocardial tissue of IR rats with EA *** infarction size was detected by TTC *** CK,creatinine kinase-myocardial band,Cardiac troponin I,and lactate dehydrogenase levels were determined by *** effect of SLC26A4 on cardiomyocyte apoptosis was explored by TUNEL staining and western *** effects of SLC26A4 on inﬂammation were determined by HE staining,ELISA,and real-time *** effect of SLC26A4 on the NF-κB pathway was determined by western ***:SLC26A4 was up-regulated in IR rats but downregulated in IR rats with EA *** with IR rats,those with SLC26A4 knockdown exhibited improved cardiac function according to decreased myocardial infarction size,reduced serum LDH/CK/CK-MB/cTnI levels,and elevated left ventricular ejection fraction and fractional ***26A4 silencing inhibited myocardial inﬂammation,cell apoptosis,phosphorylation,and nuclear translocation of NF-κB ***:SLC26A4 exhibited promoting effects on myocardial IR injury,while the SLC26A4 knockdown had an inhibitory effect on the NF-κB *** results further unveil the role of SLC26A4 in IR injury.

关键词： Myocardial ischemia Reperfusion SLC26A4 NF-κB pathway

A Compact UHF Antenna Based on Hilbert Fractal Elements and a Serpentine Arrangement for Detecting Partial Discharg

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Energy Engineering 2024年第5期121卷 1127-1141页

作者： Xiang Lin Jian Fang Ming Zhang kuang Yin Yan Tian Yingfei Guo Qianggang Wang Guangzhou Power Supply Bureau of Guangdong Power Grid Co. Ltd.China Southern Power GridGuangzhou510620China State Key Laboratory of Power Transmission Equipment Technology School of Electrical EngineeringChongqing UniversityChongqing400044China

Efforts to protect electric power systems from faults have commonly relied on the use of ultra-high frequency(UHF)antennas for detecting partial discharge(PD)as a common precursor to ***,the effectiveness of existing UHF antennas suffers from a number of challenges such as limited bandwidth,relatively large physical size,and low detection *** present study addresses these issues by proposing a compact microstrip patch antenna with fixed dimensions of 100 mm×100 mm×1.6 *** results of computations yield an optimized antenna design consisting of 2nd-order Hilbert fractal units positioned within a four-layer serpentine arrangement with a fractal unit connection distance of 3.0 ***,the optimized antenna design achieves a detection bandwidth for which the voltage standing wave ratio is less than 2 that is approximately 97.3%of the UHF frequency range(0.3–3 GHz).Finally,a prototype antenna is fabricated using standard printed circuit board technology,and the results of experiments demonstrate that the proposed antenna is capable of detecting PD signals at a distance of 8 m from the discharge source.

关键词： Power system fault partial discharge UHF antenna fractal antenna micro-strip antenna

Multifaceted role of microRNAs in gastric cancer stem cells: Mechanisms and potential biomarkers

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World Journal of Gastrointestinal Oncology 2024年第2期16卷 300-313页

作者： Qian-Hui Sun Zi-Yu kuang Guang-Hui Zhu Bao-Yi Ni Jie Li Department of Oncology Guang'anmen HospitalChina Academy of Chinese Medical SciencesBeijing 100053China Graduate College Beijing University of Traditional Chinese MedicineBeijing 100029China Department of Oncology Heilongjiang University of Chinese MedicineHarbin 150040Heilongjiang ProvinceChina

MicroRNAs(miRNAs)have received much attention in the past decade as potential key epigenomic regulators of tumors and cancer stem cells(CSCs).The abnormal expression of miRNAs is responsible for different phenotypes of gastric cancer stem cells(GCSCs).Some specific miRNAs could be used as promising biomarkers and therapeutic targets for the identification of *** review summarizes the coding process and biological functions of miRNAs and demon-strates their role and efficacy in gastric cancer(GC)metastasis,drug resistance,and apoptosis,especially in the regulatory mechanism of *** shows that the overexpression of onco-miRNAs and silencing of tumor-suppressor miRNAs can play a role in promoting or inhibiting tumor metastasis,apart from the initial formation of *** also discusses the epigenetic regulation and potential clinical applications of miRNAs as well as the role of CSCs in the pathogenesis of *** believe that this review may help in designing novel therapeutic approaches for GC.

关键词： Gastric cancer,Cancer stem cells MicroRNAs Epigenetics Therapeutic target

Enhancing the Photocatalytic Activity of Anatase TiO2 by Improving the Specific Facets-Induced Spontaneous Separation of Photogenerated Electrons and Holes

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Enhancing the Photocatalytic Activity of Anatase TiO2 by Imp...

CHCHD2 Thr61Ile mutation impairs F1F0-ATPase assembly in in vitro and in vivo models of Parkinson's disease

中国化学会第六届全国结构化学学术会议

作者： chang liu,Qin kuang,~* Zhao-Xiong Xie,~* Lan-Sun Zheng State Key Laboratory for Physical Chemistry of Solid Surfaces & Department of Chemistry,College of Chemistry and Chemical Engineering,Xiamen University,Xiamen, 361005(China)

Recently,it has been proved that directional flow of photogenerated charge carriers occur on the specific facets of TiO *** this paper,we demonstrated that photocatalytic activity of anatase TiO nanocrystals in both photoreduction and photooxidation processes can be enhanced by selectively depositing Pt nanoparticles on the {101} facets,which strengthens spontaneously surface-induced

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Neural Regeneration Research 2024年第1期19卷 196-204页

作者： Xiang Chen Yuwan Lin Zhiling Zhang Yuting Tang Panghai Ye Wei Dai Wenlong Zhang Hanqun liu Guoyou Peng Shuxuan Huang Jiewen Qiu Wenyuan Guo Xiaoqin Zhu Zhuohua Wu Yaoyun kuang Pingyi Xu Miaomiao Zhou Department of Neurology The First Affiliated Hospital of Guangzhou Medical UniversityGuangzhouGuangdong ProvinceChina Department of Neurology The People’s Hospital of Guangxi Zhuang Autonomous RegionNanningGuangxi Zhuang Autonomous RegionChina School of Basic Medical Sciences Guangzhou Medical UniversityGuangzhouGuangdong ProvinceChina Department of Neurology Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghaiChina

Mitochondrial dysfunction is a significant pathological alte ration that occurs in Parkinson's disease(PD),and the Thr61lle(T61I)mutation in coiled-coil helix coiled-coil helix domain containing 2(CHCHD2),a crucial mitochondrial protein,has been reported to cause Parkinson's ***-ATPase participates in the synthesis of cellular adenosine triphosphate(ATP)and plays a central role in mitochondrial energy ***,the specific roles of wild-type(WT)CHCHD2 and T611-mutant CHCHD2 in regulating F1FO-ATPase activity in Parkinson's disease,as well as whether CHCHD2 or CHCHD2 T61I affects mitochondrial function through regulating F1FO-ATPase activity,remain unclea ***,in this study,we expressed WT CHCHD2 and T61l-mutant CHCHD2 in an MPP^(+)-induced SH-SY5Y cell model of *** found that CHCHD2 protected mitochondria from developing MPP^(+)-induced *** normal conditions,ove rexpression of WT CHCHD2 promoted F1FO-ATPase assembly,while T61I-mutant CHCHD2 appeared to have lost the ability to regulate F1FO-ATPase *** addition,mass spectrometry and immunoprecipitation showed that there was an interaction between CHCHD2 and *** weeks after transfection with AAV-CHCHD2 T61I,we intraperitoneally injected 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine into mice to establish an animal model of chronic Parkinson's disease and found that exogenous expression of the mutant protein worsened the behavioral deficits and dopaminergic neurodegeneration seen in this *** findings suggest that WT CHCHD2 can alleviate mitochondrial dysfunction in PD by maintaining F1F0-ATPase structure and function.

关键词： ATP synthase(F1F0-ATPase) coiled-coil helix coiled-coil helix domain containing 2 dopaminergic neuron mitochondrial dysfunction neurodegeneration oligomycin sensitivity-conferring protein Parkinson's disease T61I mutation tyrosine hydroxylase

Diagnostic approaches for monkeypox virus

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iLABMED 2024年第1期2卷 6-13页

作者： Guohao Fan Jiahua kuang Shengjie Zhang Yang Yang Yingxia liu Hongzhou Lu Shenzhen Key Laboratory of Pathogen and Immunity Shenzhen Clinical Research Center for Infectious DiseaseState Key Discipline of Infectious DiseaseShenzhen Third People's HospitalSecond Hospital Affiliated to Southern University of Science and TechnologyShenzhenChina National Clinical Research Center for Infectious Disease ShenzhenChina

Mpox(formerly Monkeypox)is a zoonotic infection caused by Monkeypox virus(MPXV).Since 2022,Mpox epidemics have occurred in many nonendemic countries and regions,leading the World Health Organization to declare a public health emergency of international *** the persistent transmission and evolution of MPXV,symptoms of Mpox have become milder,with some infections being *** addition,MPXV has become more ***,rapid and accurate diagnosis and screening of MPXV is vital to prevent and control MPXV ***,we review and summarize the technical details,application scenarios,and the advantages and disadvantages of MPXV‐specific diagnostic methods.

关键词： antigen diagnosis laboratory testing monkeypox virus specimens

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煤矿钢井架结构设计要点探讨

煤炭工程 2024年第9期56卷 31-35页

作者：刘矿中国煤炭科工集团北京华宇工程有限公司北京100120

钢井架因良好的结构性能在大中型煤矿的竖向提升中被广泛应用。为了研究四斜柱式钢井架设计的布置选型、受力分析、荷载组合等设计要点,以实际工程为例,对同一模型分别采用不同规范的分项系数进行计算,并对结果进行对比。结果表明,按照... 详细信息

钢井架因良好的结构性能在大中型煤矿的竖向提升中被广泛应用。为了研究四斜柱式钢井架设计的布置选型、受力分析、荷载组合等设计要点,以实际工程为例,对同一模型分别采用不同规范的分项系数进行计算,并对结果进行对比。结果表明,按照新规范的分项系数进行设计更安全,同时提出了重视前期方案设计、进行参数化、模块化设计的建议,研究结果可供类似工程参考对照。

关键词：钢井架破断力工作荷载分项系数应力比

Intelligent Shipbuilding: Into the Main Battefield of Digital Transformation

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船舶经济贸易 2024年第1期 71-76页

作者： kuang Zhanting 不详

Whattechnical routeto be taken to promote the new type of industrialization and accelerate the building of a manufacturing and shipbuilding power?The consensus of industry experts is:taking intelligent manufacturing(IM)as the main *** present,accelerating the intelligent transformation has becomean inevitable choice for China's shipbuilding industry to promote the transformation and upgrading,and realize high-quality *** theinitiatory automation equipment application and information technology(IT)promotion,to today,the intelligent transformation of China's shipbuilding industry has entered a new stage of development.

关键词： Transformation promotion industry