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Genetic variation in relation to adaptability of three mangrove species from the Indian Sundarbans assessed with RAPD and ISSR markers

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Journal of Forestry Research 2018年第2期29卷 300-309页

作者： Nirjhar Dasgupta Paramita Nandy chandan sengupta Sauren Das Agricultural and Ecological Research Unit Indian Statistical Institute Deaprtment of Botany Sister Nibedita Government General Degree College for Girls' Department of Botany University of Kalyani

Rich genetic polymorphism is important for plants to adapt to changes because it enables the plant to make anatomical,physiological and biochemical changes in response to abiotic *** characteristics,demographic interference and a cumulative decrease in freshwater influx in the Indian Sundarbans region have proved detrimental to some economically important *** this study,genetic polymorphism of three mangrove species,Xylocarpus granatum,Excoecaria agallocha,and Phoenix paludosa,was assessed using RAPD and ISSR molecular ***,already in distress in the Sundarbans,had the least genetic polymorphism,14.56%in the RAPD analysis and 12.92% in the *** higher genetic polymorphism was recorded for the profusely growing *** and ***: 24.66 and26.4% in RAPD; 24.87 and 20.32% in ISSR analysis respectively.A UPGMA dendrogram constructed using the similarity matrix from RAPD,ISSR and combined datashowed that for ***,the least and highest salinity zones clustered together,whereas for *** and ***,higher and lower salinity areas clustered in different ***’s genetic diversity,calculated from RAPD and ISSR data,was also in accordance with 0.0637 and 0.0583 for ***,respectively,much lower than0.0794 and 0.0818 for *** and 0.0799 and 0.0688 for *** opposing degree of polymorphism might be attributed to the profusely growing *** and *** and precarious status of *** throughout the Indian Sundarbans.

关键词： Polymorphism ISSR Mangrove RAPD Sundarbans

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<i>In Vitro</i>Propagation of Ocimum Gratissimum L. (Lamiaceae) and Its Evaluation of Genetic Fidelity Using RAPD Marker

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American Journal of Plant Sciences 2012年第1期3卷 64-74页

作者： Soumen Saha Abdul Kader chandan sengupta Parthadeb Ghosh 不详

An efficient plant propagation system through nodal explants was established in Ocimum gratissimum L, a medicinally important herbaceous perennial herb belonging to the family Lamiaceae. Axillary shoot bud proliferation was initiated from nodal explants cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of N6-benzyladenine (BA) (0.5 - 3.0 mg/l), Kinetin (KN) (0.5 - 3.0 mg/l) and 2-isoPentenyladenine (2-iP) (0.5 - 3.0 mg/l). Maximum numbers of shoots (5.17 ± 0.04) with average length (2.50 ± 0.07) were induced on medium containing 1.0 mg/l BA. Shoot multiplication was maintained by repeated subculturing the original nodal explants on shoot multiplication medium after each harvest of newly formed shoots. Histological study shows that the organogenesis occurs directly, without callus formation on epidermal and sub epidermal layer of the explants. Rooting of shoots was achieved on half strength MS medium supplemented with 1.5 mg/1 Indole-3-butyric acid (IBA) and 2% sucrose. Well-developed complete plantlets were transferred to plastic pots containing a mixture of (1:1) soil and vermiculite showed 82.5 % survival rate. Genetic fidelity was assessed by chromosome analysis and DNA fingerprinting using random amplified polymorphic DNA (RAPD) of in vitro and in vivo plants. Nine arbitrary decamers displayed same banding profile showed no genomic alterations, indicating homogeneity among the tissue culture regenerates and genetic uniformity with that of donor plants. The present study provides high fidelity micro-propagated system for efficient and rapid micro-propagation protocol of this important medicinal plant and great use in conserving without risk of genetic instability.

关键词： Micropropagation Multiple Shoot Nodal Explant Histology Genetic Fidelity

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Health-Benefit Assets of Different Indian Processed Teas: A Comparative Approach

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American Journal of Plant Sciences 2017年第7期8卷 1607-1623页

作者： Anjan Hazra Jhinuk Saha Nirjhar Dasgupta chandan sengupta Padmanabhan Mohan Kumar Sauren Das Agricultural and Ecological Research Unit Indian Statistical Institute Kolkata India Department of Botany University of Kalyani Kalyani India Tea Board of India National Tea Research Foundation Kolkata India

A comparative deliberation has been considered among three Indian processed teas (black, green and white) in respect to their prevalence of some secondary metabolites, antioxidant ability (ABTS and DPPH assay), nutritional properties, inorganic elemental profile and bactericidal efficiency. Green and white tea, incidence of total phenol, total flavonoids, proanthocyanidins and tannin are higher than the black. ABTS and DPPH study reveals lower IC50 occurred in Green tea. Green tea is enriched in Na, Fe, Mg, and Mn content;black is rich in K, Ca and white has highest Zn. Total sugar and free amino acid are highest in white tea;total protein content is almost same in all three types. Green tea is enriched with vitamin C. Antimicrobial asset is experienced against Bacillus subtilis, Staphylococcus aureus (gram + ve) and Escherichia coli and Pseudomonas aeruginosa (gram?-?ve) bacteria. Study revealed that green tea has higher antimicrobial activity than the other two, though higher inhibitory effect of black tea might be attributed to the presence of substantial amount of tannin. Statistical evaluation reflects that in all organic and aqueous extracts, secondary metabolites correlate linearly with DPPH and ABTS assays but are not consistent with bactericidal efficiency in all cases.

关键词： Antioxidant Bactericidal Properties Dietary Assessment Minerals Secondary Metabolites Tea

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Health-Benefit Assets of Different Indian Processed Teas: A Comparative Approach

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American Journal of Plant Sciences（美国植物学期刊） 2017年第7期

作者： Anjan Hazra Jhinuk Saha Nirjhar Dasgupta chandan sengupta Padmanabhan Mohan Kumar Sauren Das

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