检索结果-南通市图书馆

山东交通科技 2017年第4期 61-64页

作者：李雯婵王安邦山东省交通科学研究院山东济南250031 宁阳县交通运输局山东泰安271400

主要对加德士沥青、青川岩沥青改性沥青、SBS改性沥青混合料动态模量进行了研究。

关键词：岩沥青掺量 SBS改性沥青

Polymorphisms in interleukin-lB(IL-1B) and interleukin 1 receptor antagonist(IL-1RN) genes associate with gastric cancer risk in Chinese

在线全文

学校读者我要写书评

暂无评论

Polymorphisms in interleukin-lB(IL-1B) and interleukin 1 rec...

The Therapeutic Effect of Pamidronate on Lethal Avian Influenza A H7N9 Virus Infected Humanized Mice

2010’全国肿瘤分子标志及应用学术研讨会暨第五届中国中青年肿瘤专家论坛

作者： bang-Shun He,Yu-Qin Pan,Yong-Fei Xu,chan Zhu,Li-Li Qu,Shu-Kui Wang Central Laboratory,Nanjing First Hospital Affiliated to Nanjing Medical University,Nanjing 210006,China.

Background:Gastric cancer is one of the most common malignancies afflicting the Chinese *** in interleukin-1B(IL-1B) and interleukin-1 receptor antagonist(IL-1RN) genes have been associated with increased gastric cancer

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

The Therapeutic Effect of Pamidronate on Lethal Avian Influe...

视网膜神经纤维层厚度与糖尿病视网膜病变程度的相关性研究

第十届全国免疫学学术大会

作者： Jian Zheng Wai-Lan Wu Yinping Liu Zheng Xiang Ming Liu Kwok-Hung chan Siu-Ying Lau Kwok-Tai Lam Kelvin Kai-Wang To Joseph Fuk-woo chan Lanjuan Li Honglin Chen Yu-Lung Lau Kwok-Yung Yuen Wenwei Tu Department of Paediatrics & Adolescent Medicine University of Hong Kong State Key Laboratory of Emerging Infectious Diseases Department of Microbiology University of Hong Kong Guangzhou Institute of Respiratory Diseases Guangzhou Medical University State Key Laboratory for Diagnosis and Treatment of Infectious Diseases first Affiliated HospitalCollege of Medicine Zhejiang University

Objective:A novel avian influenza virus H7N9 infection occurred among human populations since *** thelack of sustained human-to-human transmission limited the epidemics caused by H7N9,the late presentation of most patients and the emergence of neuraminidase-resistant strains made the development of novel antiviral strategy against H7N9 in urgent *** this study,we evaluated the potential of pamidronate,a pharmacological phosphoantigen that can specifically boost human Vdelta2-T-cell,on treating H7N9 virus-infected humanized ***:H7N9-infected humanized mice were treated with a human equivalent dose of pamidronate or saline of equivalent volume via injected intraperitoneal injection,or oseltamivir or saline of equivalent volume by oral *** weight change and survival of infected mice were monitored *** indicated time points,lungs from different groups were harvested for pathology examination,while the viral loads and the levels of human pro-inflammatory cytokines and chemokines in the supernatants of homogenized lung tissue were ***:Our results showed that intraperitoneal injection of pamidronate could potently decrease the morbidity and mortality of H7N9-infected mice through controlling both viral replication and inflammation in affected *** importantly,pamidronate treatment starting from 3 days after infection could still significantly ameliorate the severity of diseases in infected mice and improve their survival chance,whereas orally oseltamivir treatment starting at the same time showed no therapeutic *** for the mechanisms underlying pamidronate-based therapy,our in vitro data demonstrated that its antiviral effects were mainly mediated by human Vdelta2-T-cell through their secretion of IFN-γand cytotoxicity against virus-infected host cells dependent on the expressions of perforin,granzyme B and ***:As pamidronate has been used for osteoporosis treatment for more than 20 years,pamidr

关键词： Vdelta2-T cell Amidronate Oseltamivir Humanized mice

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

眼科新进展 2011年第5期31卷 438-440页

作者：熊小艳毛新帮王婵婵赵菊莲游志鹏陆莹南昌大学第二附属医院眼科江西省南昌市330006

目的通过光学相干断层扫描(optical coherence tomography,OCT)仪观察具有不同视网膜病变程度的2型糖尿病患者及正常人视网膜神经纤维层(retinal nerve fiber layer,RNFL)厚度,探讨RNFL厚度与糖尿病视网膜病变(diabetic retinopathy,DR)的程度是否有相关性。方法正常对照组50人89眼,2型糖尿病患者75例130眼,按DR国际临床分型标准分为无糖尿病视网膜病变(non-diabetic retinopathy,NDR)组37例70眼、非增生性糖尿病视网膜病变(non-proliferative diabetic retinopathy,NPDR)组21例33眼、增生性糖尿病视网膜病变(proliferative diabetic retinopathy,PDR)组17例27眼,所有患者均行眼科常规检查和OCT检查;OCT测量数据包括:视盘周围360°平均及上方、下方、鼻侧和颞侧平均RNFL厚度。结果正常对照组RNFL厚度视盘360°平均为(106.80±9.28)μm、上方(130.24±16.13)μm、下方(137.50±14.12)μm、鼻侧(75.02±8.90)μm、颞侧(84.20±12.39)μm;NDR组RNFL厚度360°平均为(104.20±7.12)μm、上方(120.46±20.40)μm、下方(137.63±11.18)μm、鼻侧(74.42±6.50)μm、颞侧(84.38±12.58)μm;NPDR组RNFL厚度360°平均为(86.67±4.22)μm、上方(105.97±7.22)μm、下方(114.97±5.29)μm、鼻侧(52.93±7.86)μm、颞侧(72.81±6.91)μm;PDR组RNFL厚度360°平均为(127.92±6.44)μm、上方(154.48±9.28)μm、下方(167.22±9.59)μm、鼻侧(83.33±9.83)μm、颞侧(106.63±10.40)μm。与正常对照组相比,NPDR、PDR组各方位RNFL厚度差异均有统计学意义(均为P<0.05),NPDR组变薄而PDR组变厚;NDR组与正常对照组比较,只有360°平均及上方有统计学意义(均为P<0.05);NDR、NPDR、PDR三组间两两比较,差异均有统计学意义(均为P<0.05)。结论糖尿病患者RNFL厚度的变化与DR的程度具有相关性,NDR及NPDR患者RNFL厚度变薄提示眼底开始出现神经节细胞凋亡的可能,当RNFL厚度由薄转为增厚则提示出病变的进行性加重,对临床早期诊断及治疗具有指导意义。

关键词：视网膜神经纤维层厚度糖尿病视网膜病变光学相干断层扫描

A six-year descriptive epidemiological study of human coronavirus infections in hospitalized patients in Hong Kong

在线全文

学校读者我要写书评

暂无评论

Virologica Sinica 2016年第1期31卷 41-48页

作者： Cyril C.Y.Yip Carol S.F.Lam Hayes K.H.Luk Emily Y.M.Wong Rodney A.Lee Lok-Yee So Kwok-Hung chan Vincent C.C.Cheng Kwok-Yung Yuen Patrick C.Y.woo Susanna K.P.Lau Department of Microbiology The University of Hong Kong Department of Microbiology Pamela Youde Nethersole Eastern Hospital Department of Paediatrics and Adolescent Medicine Pamela Youde Nethersole Eastern Hospital Research Centre of Infection and Immunology The University of Hong Kong State Key Laboratory of Emerging Infectious Diseases The University of Hong Kong

We conducted a six-year epidemiological study on human coronaviruses（HCoVs） circulating in Hong Kong, using 8275 nasopharyngeal samples from patients with acute respiratory tract infections. HCoVs were detected in 77（0.93%） of the samples by a pan-HCoV RT-PCR assay. The most frequently detected HCoV species was HCoV-OC43（0.58%）, followed by HCoV-229E（0.15%）,HCoV-HKU1（0.13%） and HCoV-NL63（0.07%）. HCoVs were detected throughout the study period（September 2008–August 2014）, with the highest detection rate from September 2010 to August2011（22/1500, 1.47%）. Different seasonal patterns of each HCoV species in Hong Kong were ***-OC43 was predominant in the fall and winter, whereas HCoV-HKU1 showed peak activity in winter, with a few cases occurred in spring and summer. HCoV-229 E mainly occurred in winter and spring, while HCoV-NL63 was predominant in summer and autumn. HCoVs most commonly infect the elderly and young children, with median age of 79.5 years（range, 22 days to 95 years）.Intriguingly, the detection rate of HCoV-OC43 in the age group of ＆gt; 80 years（26/2380, 1.09%） was significantly higher than that in the age group of 0–10 years（12/2529, 0.47%）（P 〈 0.05）. These data provides new insight into the epidemiology of coronaviruses.

关键词： epidemiology human coronaviruses respiratory tract infections

在线全文

学校读者我要写书评

暂无评论

我国古代农禅的特征

安徽农业科学 2008年第23期36卷 10272-10273页

作者：李帮儒郑州大学历史学院

综述了我国古代农禅的3个主要特征。

关键词：禅宗农禅禅农

Effect of ischemic preconditioning on antioxidant status in the gerbil hippocampal CA1 region after transient forebrain ischemia

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2016年第7期11卷 1081-1089页

作者： Seung Min Park chan woo Park Tae-Kyeong Lee Jeong Hwi Cho Joon Ha Park Jae-Chul Lee Bai Hui Chen Bich-Na Shin Ji Hyeon Ahn Hyun-Jin Tae Myoung Cheol Shin Taek Geun Ohk Jun Hwi Cho Moo-Ho Won Soo Young Choi In Hye Kim Department of Emergency Medicine Sacred Heart HospitalCollege of MedicineHallym University Department of Emergency Medicine School of MedicineKangwon National University Department of Neurobiology School of MedicineKangwon National University Department of Physiology College of MedicineHallym University Department of Biomedical Science Research Institute of Bioscience and BiotechnologyHallym University

Ischemic preconditioning（IPC） is a condition of sublethal transient global ischemia and exhibits neuroprotective effects against subsequent lethal ischemic ***,in this study,examined the neuroprotective effects of IPC and its effects on immunoreactive changes of antioxidant enzymes including superoxide dismutase（SOD） 1 and SOD2,catalase（CAT） and glutathione peroxidase（GPX） in the gerbil hippocampal CA1 region after transient forebrain *** neurons of the stratum pyramidale（SP） in the hippocampal CA1 region of animals died 5 days after lethal transient ischemia without IPC（8.6%（ratio of remanent neurons） of the sham-operated group）;however,IPC prevented the pyramidal neurons from subsequent lethal ischemic injury（92.3%（ratio of remanent neurons） of the sham-operated group）.SOD1,SOD2,CAT and GPX immunoreactivities in the sham-operated animals were easily detected in pyramidal neurons in the stratum pyramidale（SP） of the hippocampal CA1 region,while all of these immunoreactivities were rarely detected in the stratum pyramidale at 5 days after lethal transient ischemia without ***,their immunoreactivities in the sham-operated animals with IPC were similar to（SOD1,SOD2 and CAT） or higher（GPX） than those in the sham-operated animals without ***,their immunoreactivities in the stratum pyramidale of the ischemia-operated animals with IPC were steadily maintained after lethal ischemia/*** of western blot analysis for SOD1,SOD2,CAT and GPX were similar to immunohistochemical *** conclusion,IPC maintained or increased the expression of antioxidant enzymes in the stratum pyramidale of the hippocampal CA1 region after subsequent lethal transient forebrain ischemia and IPC exhibited neuroprotective effects in the hippocampal CA1 region against transient forebrain ischemia.

关键词： hippocampal operated antioxidant stratum subsequent superoxide glutathione neuronal hippocampus peroxidase

Infectious intracranial aneurysm associated with Lactococcus garvieae: A case report and literature review

在线全文

学校读者我要写书评

暂无评论

Infectious Medicine

Infectious Medicine 2024年第3期3卷 78-86页

作者： Chung-Ho Lee Peter Yat-Ming woo Calvin Ka-Lam Leung Ronald Li Jenny Kwan-Tsz chan Kwan-Shun Ng Cindy Wing-Sze Tse Department of Pathology Kwong Wah HospitalHong Kong Special Administrative RegionChina Department of Neurosurgery Kwong Wah HospitalHong Kong Special Administrative RegionChina

Lactococcus garvieae is a known fish pathogen associated with numerous aquacultural *** humans,*** primarily causes infective endocarditis,but infections involving other organs have also been *** report the first case of ruptured infectious intracranial aneurysm associated with *** bacteraemia without concomitant infective *** diagnosis of a left distal posterior cerebral artery mycotic aneurysm was based on a computed tomography angiogram,catheter angiogram and histopathological examination of the resected ***,we review the literature on human *** infections and describe the clinical characteristics,risk factors,management and outcomes of the cases identified to date.

关键词： Lactococcus garvieae Bacteraemia Infectious intracranial aneurysm Mycotic aneurysm Subarachnoid haemorrhage

重组腺病毒Ad-DT-A靶向治疗胰岛素样生长因子2基因组印迹丢失的肿瘤细胞株

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中华肿瘤杂志 2011年第11期33卷 816-821页

作者：潘玉琴何帮顺朱蝉渠利利徐勇飞王书奎南京医科大学附属南京第一医院中心实验室 210006 南京师范大学生命科学学院

目的构建携带胰岛素样生长因子2（IGF2）印迹系统的重组腺病毒，探讨其用于肿瘤靶向治疗的可行性。方法采用聚合酶链反应（PCR）扩增H19enhancer、DMD以及H19启动子序列，并克隆至pDC-312中；扩增出增强型绿色荧光蛋白（EGFP）和白喉毒... 详细信息

目的构建携带胰岛素样生长因子2（IGF2）印迹系统的重组腺病毒，探讨其用于肿瘤靶向治疗的可行性。方法采用聚合酶链反应（PCR）扩增H19enhancer、DMD以及H19启动子序列，并克隆至pDC-312中；扩增出增强型绿色荧光蛋白（EGFP）和白喉毒素A（DT．A）片段，分别插入到构建好的重组质粒中，构建成重组腺病毒穿梭质粒。重组腺病毒穿梭质粒与Ad5共转染，产生重组腺病毒Ad—EGFP和Ad—DT—A。以Ad—EGFP分别感染正常IGF2印迹（MOI）细胞GES．1和MCF-7以及IGF2印迹丢失（LOI）细胞HCT-8，荧光显微镜下观察各组细胞中EGFP的表达。通过检测腺病毒衣壳蛋白hexon基因的表达证实腺病毒的感染，采用逆转录聚合酶链反应（***）和Western blot方法检测Ad—DT—A感染后各组细胞中DT—A基因的表达；采用四甲基偶氮唑蓝（M1Tr）法和流式细胞术检测Ad—DT—A体外抗肿瘤效应。构建皮下移植瘤裸鼠模型，研究Ad-DT-A在体内的抑瘤作用。结果所构建重组腺病毒Ad-EGFP感染各组细胞后，EGFP蛋白仅在IGF2LOI细胞HCT。8中表达。各组细胞经Ad—DT—A感染后，DT-A基因仅在HCT-8细胞中表达；且HCT-8细胞以每个细胞10PFU的***-A感染72h后，其增殖活力降低至（75．4±6．4）％，凋亡率升高至（20．8±5．9）％。Ad-DT-A多点注射人HCT-8移植瘤内，Ad—DT—A能够有效抑制移植瘤的生长，抑瘤率达36．4％。结论成功构建了携带IGF2印迹系统和DT-A基因的重组腺病毒。重组腺病毒Ad—DT-A能够有效杀伤IGF2LOI肿瘤细胞，为依赖IGF2LOI的肿瘤靶向治疗开拓了新的途径。

关键词： GES-1细胞 MCF-7细胞 HCT HCT-8款新产品亮相BICES2011细胞 IGF2印迹 IGF2印迹丢失靶向治疗重组腺病毒

在线全文

学校读者我要写书评

暂无评论

特征荧光谱在早期膀胱癌诊断中的应用

中华医学杂志 2007年第36期87卷 2544-2546页

作者：邹钧何慧婵姚永帮戴奇山韩兆冬钟惟德广州医学院附属广州市第一人民医院泌尿外科 510180 中国科学院安徽光学精密机械研究所

目的寻找紫外激光诱发膀胱癌组织荧光特征光谱并探讨其在膀胱癌早期诊断中的应用价值。方法以血卟啉单甲醚作为光敏剂,全固态紫外激光作为激发光源(波长355nm),检测膀胱癌组织特征荧光光谱。并对其特性进行分析研究。结果膀胱癌组织自... 详细信息

目的寻找紫外激光诱发膀胱癌组织荧光特征光谱并探讨其在膀胱癌早期诊断中的应用价值。方法以血卟啉单甲醚作为光敏剂,全固态紫外激光作为激发光源(波长355nm),检测膀胱癌组织特征荧光光谱。并对其特性进行分析研究。结果膀胱癌组织自发荧光在445～490nm处存在双峰状组织特征蜂,经光敏剂孵育后癌组织药物荧光谱除出现组织峰外还在628.65nm 处出现特征药物峰。结论紫外激光诱发膀胱癌组织药物荧光光谱性质稳定,强度佳,特征药物峰处于可见光范围,肉眼易识别,有望提高早期膀胱癌诊断的阳性率。

关键词：膀胱肿瘤光谱法荧光诊断