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Comparative analyses of the venom components in the salivary gland transcriptomes and saliva proteomes of some heteropteran insects

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Insect Science 2022年第2期29卷 411-429页

作者： Kyungjae Andrew Yoon Woo-Jin Kim Seungki lee hee-Sun Yang Byoung-hee lee Si Hyeock lee Research Institute of Agriculture and Life Sciences Seoul National UniversitySeoulKorea EntoCode Co. SeoulKorea National Institute of Biological Resources Environmental Research ComplexIncheonKorea Department of Agricultural Biology Seoul National UniversitySeoulKorea

Salivary gland-specific transcriptomes of nine heteropteran insects with distinct feeding strategies (predaceous, hematophagous, and phytophagous) were analyzed and annotated to compare and identify the venom components as well as their expression profiles. The transcriptional abundance of venom genes was verified via quantitative real-time PCR. Hierarchical clustering of 30 representative differentially expressed venom genes from the nine heteropteran species revealed unique groups of salivary gland-specific genes depending on their feeding strategy. The commonly transcribed genes included a paralytic neurotoxin (arginine kinase), digestive enzymes (cathepsin and serine protease), an anti-inflammatory protein (cystatin), hexamerin, and an odorant binding protein. Both predaceous and hematophagous (bed bug) heteropteran species showed relatively higher transcription levels of genes encoding proteins involved in proteolysis and cytolysis, whereas phytophagous heteropterans exhibited little or no expression of these genes, but had a high expression of vitellogenin, a multifunctional allergen. Saliva proteomes from four representative species were also analyzed. All venom proteins identified via saliva proteome analysis were annotated using salivary gland transcriptome data. The proteomic expression profiles of venom proteins were in good agreement with the salivary gland-specific transcriptomic profiles. Our results indicate that profiling of the salivary gland transcriptome provides important information on the composition and evolutionary features of venoms depending on their feeding strategy.

关键词： hematophagous heteroptera phytophagous predaceous proteome analysis salivary gland-specific venom transcriptome analysis

Effectiveness and safety of auriculotherapy for breastfeeding: a systematic review

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Journal of Traditional Chinese Medicine 2020年第5期40卷 721-737页

作者： Boram lee chan-Young Kwon Sun Haeng lee Department of Clinical Korean Medicine Graduate SchoolKyung Hee UniversitySeoul 02453Republic of Korea Clinical Medicine Division Korea Institute of Oriental MedicineDaejeon 34054Republic of Korea Department of Oriental Neuropsychiatry Dong-eui University College of Korean MedicineBusan 47227Republic of Korea Department of Pediatrics of Korean Medicine Kyung Hee University Korean Medicine HospitalKyung Hee University Medical CenterSeoul 02447Republic of Korea

OBJECTIVE:To summarize and critically evaluate the evidence pertaining to the effectiveness and safety of auriculotherapy for ***:We performed a systematic search of10 databases from their inception dates to May 15,*** controlled trials evaluating the effectiveness and safety of auriculotherapy for breastfeeding were *** of bias was assessed using the Cochrane risk of bias ***:This review included 31 randomized controlled trials with 5389 *** to the obvious clinical or statistical heterogeneity,a Meta-analysis was not ***-nine studies compared auriculotherapy plus routine care with routine care *** consistent adjunctive effect of auriculotherapy was observed in increasing the volume of milk production(9 studies showed significant results in favor of auriculotherapy and 5 did not)and serum prolactin level(7 studies showed significant results in favor of auriculotherapy and 2 did not),and in facilitating the initiation of milk secretion(10 studies showed significant results in favor of auriculotherapy and 7 did not).Two studies compared auriculotherapy with no *** as a monotherapy showed significant effects in increasing serum prolactin level and facilitating the onset of milk secretion;however,it was limited and inconclusive because only two studies were included in this *** subgroup analysis of women who underwent cesarean delivery showed inconsistent results with respect to all *** serious adverse events were reported with the use of *** methodological quality of the included studies was generally ***:Although some studies showed positive results,no definite conclusion about the effectiveness and safety of auriculotherapy for breastfeeding could be drawn,owing to the low methodological quality of the included studies and the heterogeneity among ***-scale,well-designed studies are warranted on this topic.

关键词： Breastfeeding Auriculotherapy Systematic review

Safety of acupotomy in a real-world setting:A prospective pilot and feasibility study

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Journal of Integrative Medicine 2022年第6期20卷 514-523页

作者： Sang-Hoon Yoon chan-Young Kwon hee-Geun Jo Jae-Uk Sul Hyangsook lee Jiyoon Won Su Jin Jeong Jun-Hwan lee Jungtae leem Chung-Yeon Korean Medicine Clinic Gangnam-guSeoul 06224Republic of Korea Department of Oriental Neuropsychiatry Dong-eui University College of Korean MedicineBusanjin-guBusan 47340Republic of Korea Department of Herbal Pharmacology College of Korean MedicineGachon University1342 Seongnamdae-roSujeong-guSeongnam 13120Republic of Korea Department of Korean Rehabilitation Medicine Dongshin University Korean Medicine HospitalGwangju 61619Republic of Korea Acupuncture and Meridian Science Research Center Kyung Hee UniversityDongdaemun-guSeoul 02447Republic of Korea Department of Science in Korean Medicine Graduate SchoolKyung Hee UniversityDongdaemun-guSeoul 02447Republic of Korea KM Science Research Division Korea Institute of Oriental MedicineDaejeon 34054Republic of Korea Department of Statistics Support Medical Science Research InstituteKyung Hee University Medical CenterDongdaemun-guSeoul 02447Republic of Korea Clinical Medicine Division Korea Institute of Oriental MedicineYuseong-guDaejeon 34054Republic of Korea Korean Medicine Life Science University of Science&Technology(UST)Campus of Korea Institute of Oriental MedicineYuseong-guDaejeon 34054Republic of Korea College of Korean Medicine Wonkwang UniversityIksanJeollabuk-do54538Republic of Korea

Objective:Acupotomy is a modern acupuncture method that includes modern surgical *** acupotomy is relatively more invasive than filiform acupuncture treatment,it is important to establish the safety profile of this *** justify further large-scale prospective observational studies,this preliminary study was performed to assess the feasibility of the approach and investigate the safety profile and factors potentially associated with adverse events(AEs).Methods:This was a prospective pilot study that assessed the feasibility of a large-scale forthcoming safety study on acupotomy treatment in a real-world *** feasibility(call response rate,dropout rate,response rate for each variable and recruitment per month)and safety profile(incidence,type,severity and causality of AEs,and factors potentially associated with AEs)were ***:A total of 28 participants joined the study from January to May 2018.A follow-up assessment was achieved in 258(1185 treatment points)out of 261 sessions(1214 treatment points).The response rate via telephone on the day after treatment was 87.3%.There were 8 systemic AEs in all the sessions(8/258;3.11%)and 27 local AEs on the total points treated(27/1185;2.28%).Severe AEs did not *** AE and local AE occurrence were associated with blade width and the number of needle stimulations per treatment ***:The findings suggest that it could be feasible to analyze the safety of acupotomy in a realworld ***,the primary data on some relevant AEs could be *** are planning large-scale prospective studies based on these *** registration:Clinical Research Information Service(CRIS)KCT0002849(https://***/cris/search/***/11487).

关键词： Acupotomy Acupuncture Safety Adverse effects Observational study Traditional Chinese medicine

On the Classification of Positive Quaternionic Khler Manifolds with b_4=1

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Acta Mathematica Sinica,English Series 2010年第5期26卷 875-884页

作者： Jin Hong KIM hee Kwon lee Department of Mathematical Sciences Korea Advanced Institute of Science and Technology Guseong-dong

Let M be a positive quaternionic Kahler manifold of dimension 4m. We already showed that if the symmetry rank is greater than or equal to [m/2] ＋ 2 and the fourth Betti number b4 is equal to one, then M is isometric to HPm. The goal of this paper is to report that we can improve the lower bound of the symmetry rank by one for higher even-dimensional positive quaternionic Kahler manifolds. Namely, it is shown in this paper that if the symmetry rank of M with b4（M） = 1 is greater than or equal to m/2 ＋ 1 for m ≥ 10, then M is isometric to HPm. One of the main strategies of this paper is to apply a more delicate argument of Frankel type to positive quaternionic Kahler manifolds with certain symmetry rank.

关键词： positive quaternionic Kahler symmetry

Controlled oxidation of Ni for stress-free hole transport layer of large-scale perovskite solar cells

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Nano Research 2019年第12期12卷 3089-3094页

作者： Seongha lee hee-Suk Roh Gill Sang Han Jung-Kun lee Department of Mechanical Engineering and Materials Science University of PittsburghPennsylvania 15261USA School of Advanced Materials Science and Engineering Sungkyunkwan UniversitySuwon 16419Republic of Korea

The effect of the residual thermal stress of NiO films on the performance of an inverted type perovskite solar cell was *** this study,NiO films were grown on fluorine dopedtin oxide(FTO)substrates of different surface roughness by thermally oxidizing Ni film and weretested as a hole transport layer for large-scale perovskite solar *** and simulation results show that it is very important tosuppress the appearance of the residual stress at the NiO-FTO in terface during the oxidation of the Ni film for effective hole extracti *** Ni oxidation on the flat FTO film produced in-plane compressive stress in the NiO film due to the Ni film volume *** led to theformation of defects including small *** residual stress and defects in creased leakage curre nt through the NiO film,preve ntingholes from being selectively collected at the NiO-perovskite ***,when Ni was deposited and oxidized on the rough surface,the residual stress of the NiO film was negligible and its inhere nt high resistance was ***-free NiO film is an excelle nt holetransport layer that stops the photogenerated electrons of the perovskite layer from moving to *** improvements in the structural andelectrical qualities of the NiO film by engirteering the residual stress reduce the carrier recombination and increase the power conversi onefficiency of the perovskite solar cells to 16.37%.

关键词： nickel oxidation surface roughness residual stress perovskite solar cells large scale processing

Recent TCP(Tidal Current Power) projects in Korea

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Science China(Technological Sciences) 2010年第1期53卷 57-61页

作者： Chul hee JO Kang hee lee Yu Ho RHO School of Engineering Inha University253Yonghyun-DongNam-GuIncheon 402-751Republic of Korea Graduate School Department.of Naval Architecture & Ocean EngineeringInha University253Yonghyun-DongNam-GuIncheon 402-751Republic of Korea

Having strong current on the west coast with up to 10 m tidal range in Korea,there are many suitable and applicable locations for TCP(Tidal Current Power) application in *** only the west coast,but also the south west regions having narrow channels between islands creating fast ocean stream are suitable for *** first TCP application in Korea is 1 MW helical VAT(Vertical Axis Turbine) device installed in Wooldolmog area in *** first HAT(Horizontal Axis Turbine) TCP device of 25 KW was developed and installed in cooling water weir of Samchonpo power plant in ***,a 100 kW prototype HAT TCP device is being planned to be installed in Daebang strait in *** big project would be Inchon TCP farm of 200 MW on the west coast in *** consortium of 5 members was formed in April,*** site will be the largest TCP site in the *** paper describes recent TCP projects in Korea with background and environmental conditions of the projects.

关键词： TCP(Tidal Current Power) ocean energy renewable energy clean energy HAT(Horizontal Axis Turbine) VAT(Vertical Axis Turbine)

Using fluorescence surrogates to track algogenic dissolved organic matter(AOM) during growth and coagulation/flocculation processes of green algae

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Journal of Environmental Sciences 2019年第5期31卷 311-320页

作者： Quang Viet Ly Mi-hee lee Jin Hur Department of Environment & Energy Sejong University

Tracking the variation of the algogenic organic matter(AOM) released during the proliferation of green algae and subsequent treatment processes is crucial for constructing and optimizing control strategies. In this study, the potential of the spectroscopic tool was fully explored as a surrogate of AOM upon the cultivation of green algae and subsequent coagulation/flocculation(C/F) treatment processes using ZrCl_4 and Al_2(SO_4)_3. Fluorescence excitation emission matrix coupled with parallel factor analysis(EEM-PARAFAC) identified the presence of three independent fluorescent components in AOM, including protein-like(C1), fulvic-like(C2) and humic-like components(C3). Size exclusion chromatography(SEC) revealed that C1 in AOM was composed of large-sized proteins and aromatic amino acids. The individual components exhibited their unique characteristics with respect to the dynamic changes. C1 showed the highest correlation with AOM concentrations(R^2= 0.843) upon the C/F processes. C1 could also be suggested as an optical predictor for the formation of trihalomethanes upon the C/F processes. This study sheds a light for the potential application of the protein-like component(C1) as a practical surrogate to track the evolution of AOM in water treatment or wastewater reclamation systems involving Chlorella vulgaris green algae.

关键词： EEM-PARAFAC Enhanced coagulation Size exclusion chromatography Algal dissolved organic matter Trihalomethanes

Proximate and mineral components of Viscum album var. coloratum grown on eight different host tree species

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Journal of Forestry Research 2019年第4期30卷 1245-1253页

作者： Chul-Woo Kim chan-Hoon An Hyun-Seok lee Jae-Seon Yi Eun Ju Cheong Sang-Hyun Lim hee-Yeon Kim Forest Bioresources Department National Institute of Forest Science39 OnjeongroGwonseon-guSuwon 16631Republic of Korea Forest Medicinal Resources Research Center National Institute of Forest ScienceSobaek-ro 2009PunggiYoungjuGyungsangbuk-do 36040Republic of Korea Program of Forest Resources Division of Forest ScienceCollege of Forest and Environmental SciencesKangwon National UniversityChuncheon 24341Republic of Korea Agriproduct Processing Experiment Station Gangwon-do Agicultural Research and Experiment ServicesChuncheon 24203Republic of Korea

Mistletoes are parasite plants growing on various tree species,taking nutrients from the *** compounds of mistletoe were used in medicine and these mistletoes were commonly collected from oak species that were not well *** analyzed the nutritional value by measuring proximate and mineral components of Korean mistletoe(Viscum album ***)from various host *** was the most proximate component in both leaves and twigs followed crude protein,crude fiber,crude ash and crude *** the contents of the proximate components and minerals varied among hosts and between plant parts,leaves contained higher crude protein and ash,and more minerals except for magnesium than twigs,although no significant difference was found inmistletoe grown on Prunus *** were significant differences of proximate and mineral contents depending on the host *** grown on Chaenomeles sinensis contained higher levels of carbohydrates,crude fat,crude ash,magnesium,calcium,sodium,iron,and zinc than mistletoe grown on other *** grown on *** contained high mineral levels of potassium,copper and *** nutritional value of mistletoes were greatly influenced by the host species but among the hosts,specific nutrients accumulated in leaves more than in twigs,and vice *** on the analysis,Korean mistletoe contains relatively good quality of nutrients and has high potential for dietary supplements as well as for medicinal compounds.

关键词： Mistletoe Viscum album var coloratum Proximate compositions Mineral contents Nutritional value Host species

^(27)Al MAS-NMR study of inorganic polymer formation at ambient temperature

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Transactions of Nonferrous Metals Society of China 2011年第A1期21卷 182-187页

作者： Sang-Wook AHN hee-Soo lee Wan-hee YANG Jung-Woo lee Young-Keun JEONG National Core Research Center for Hybrid Materials Solution Pusan National University School of Material Science and Engineering Pusan National University INTChem Co. Ltd.

Inorganic polymers are a novel class of materials formed by the polymerization of silicon, aluminium and oxygen species to form an amorphous three-dimensional framework structure. The basis of this process is the alkaline solutions to induce a certain amount of Si and AI atoms to dissolve from a feedstock such as aluminosilicate. A study of 27A1 MAS-NMR was carried out in an attempt to understand the reaction mechanism of the inorganic polymerization at ambient temperature. Scanning electron microscopy （SEM） and X-ray diflYactometry （XRD） were also employed to establish the composition and microstructure of the inorganic polymerization. Specimens were prepared with different A1/Si mole ratios from the starting materials. The higher the AI content, the more sufficient the AI atoms that can combine with SiO4, and the longer the reaction time, the more the bonded Si--O--A1--O polymer structure, and then the higher the A1 content, the fewer the octahedral A1 with a uniform Si--O--A1--O structure in four directions, because four AI atoms are combined with SiO4, resulting in a uniform Si--O--AI--O structure in four directions. The results show that they have an amorphous microstructure.

关键词： inorganic polymer amorphous tetrahedral