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Desmarestia anceps Montagne (Phaeophyceae) against Colorectal Cancer Cells: Cytotoxic Activity and Proapoptotic Effects

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Advances in Biological Chemistry 2022年第6期12卷 228-245页

作者： Rafaele Frassini Daniela Steffens Sidnei Moura cesar aguzzoli Aline Paternostro Martins Pio Colepicolo Mutue Toyota Fujii Nair S. Yokoya Cláudio Martin Pereira De Pereira Ana Cláudia Phillipus Miriam De Barcellos Falkenberg João Antonio Pêgas Henriques Mariana Roesch-Ely Laboratory of Applied Toxicology and Bioproducts University of Caxias do Sul RS Brazil Laboratory of Biosynthetic and Natural Products of Caxias do Sul RS Brazil Area of Exact Sciences and Engineering University of Caxias do Sul RS Brazil Laboratory of Biochemistry and Molecular Biology of Algae University of S&#227 o Paulo S&#227 o Paulo SP Brazil Nucleus Research Phycology Institute of Botany S&#227 o Paulo SP Brazil Laboratory of Lipidomics and Biorganic-LipidomicaBio Federal University of Pelotas Pelotas RS Brazil Study Group on Natural and Synthetic Products (GEPRONAS) Federal University of Santa Catarina SC Brazil

Impairment of apoptosis promotes abnormal cellular proliferation and accumulation of genetic alterations. Identifying bioactive compounds extracted from seaweeds that induce apoptosis in cancer cells may be explored as new agents for cancer chemoprevention and/or chemotherapy. The present study aimed to determine the chemical composition and biological activity of the chloroform crude extract and fractions from Antarctic seaweed Desmarestia anceps. The chloroform extract was obtained by three consecutive macerations and the fractionation by vacuum liquid chromatography. The chemical characterization of the extract and fractions was performed through Gas Chromatography. The cytotoxicity of the crude extract and fractions was evaluated by the MTT assay. Cell death and cell cycle evaluation after 24 hours of exposure to chloroform extract were performed by flow cytometry. A total of 48 compounds were identified. The results indicate that chloroform extract and its fractions presented cytotoxic activity against HCT 116 cell line in a dose dependent-manner. Proapoptotic events were observed after chloroform extract exposition, which promoted an increase of multinucleated cells and reduced cell viability. This study was the first to explore cytotoxic potential of seaweed D. anceps fractions against HCT colorectal cancer cell line, suggesting that these macroalgae may be a promising candidate against anticancer activity.

关键词： Antarctic Seaweeds Antitumor Activity Apoptosis Proapoptotic Effect Colorectal Cancer

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