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The thr.e-dimensional str.ctur. of Infectious flacher.e vir.s cap.id deter.ined by cr.o-electr.n micr.scop.

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Science china(Life Sciences) 2009年第12期52卷 1186-1191页

作者： XIE Li1,4, ZHANG QinFen2., LU XingMeng3, DAI XinHong2. LI Kunp.ng2. HONG Jian1,4· & ZHOU Xuep.ng11 Institute of Biotechnology, zhejiang university, Hangzhou 31002., china 2.State Key Labor.tor. of Biocontr.l, School of Life Sciences, Sun Yat-Sen {2., Guangzhou 5102.5, china 3 College of Animal Sciences, zhejiang university, Hangzhou 31002., china 4 center of analysis and measurement, zhejiang university, Hangzhou 31002., china Institute of Biotechnology Zhejiang University Hangzhou 310029 China State Key Labor.tor. of Biocontr.l School of Life Sciences Sun Yat-Sen University Guangzhou 510275 China College of Animal Sciences Zhejiang University Hangzhou 310029 China center of analysis and {2.ment Zhejiang University Hangzhou 310029 China

Cr.o-electr.n micr.scop. and image r.constr.ction wer. used to deter.ine the thr.e-dimensional str.ctur. of Infectious flacher.e vir.s (IFV). 5047 p.r.icles wer. selected for.the final r.constr.ction. The FSC cur.e showed that the r.solution of this cap.id str.ctur. was 18 ·. The str.ctur. is a p.uedo T=3 (p.3) icosahedr.l cap.id with a diameter.of 302.4 · and a single shell thickness of 15 ·. The density map.showed that IFV has a smooth sur.ace without any p.ominent p.otr.de or.dep.ession. Comp.r.son of the IFV str.ctur. with those of the insect p.cor.a-like vir.s-Cr.cket p.r.lysis vir.s (Cr.0})and human p.cor.avir.s-Human r.inovir.s 14 (Hr. 14) r.vealed that the IFV str.ctur. r.sembles the Cr.0} str.ctur.. The "r.ssmann canyon" is absent in both IFV and Cr.0} p.r.icles. The p.lyp.p.ide top.logy of IFV Vp.0} IFV Vp. was p.edicted and the subunit location at the cap.id sur.ace was fur.her.analyzed.

关键词： Infectious flacher.e vir.s cr.o-electr.n micr.scop. thr.e-dimensional str.ctur. p.lyp.p.ide folding

A new family of windows——convolution windows and their.ap.lications

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Science china(Technological Sciences) 2005年第4期48卷 468-480页

作者： ZHANG Jieqiu1,2. LIANG Changhong1 & CHEN Yanp.3 1. Dep.r.ment of Ap.lied Math. & p.ys., College of Science, Air.or.e Engineer.ng university, Xi’an 710051, china 2. National Key Labor.tor. of Antennas and Micr.wave Technology, Xidian university, Xi’an 710071, china 3. Comp.ter.center, Xi’an Communication College, Xi’an 710106, china 1. Dep.r.ment of Ap.lied Math. & p.ys. College of Science Airforce Engineering University 710051 Xi’an China 2. National Key Labor.tor. of Antennas and Micr.wave Technology Xidian University 710071 Xi’an China 3. Comp.ter.center Xi’an Communication College 710106 Xi’an China

A new family of windows is constr.cted by convolutions via a few r.ctangular.windows with same time width and is thus r.fer.ed to as convolution windows. The exp.essions of the second-or.er.up.to the eighth-or.er.convolution windows in both the time and fr.quency domains ar. der.ved. Their.ap.lications in high accur.cy har.onic analysis of p.r.odic signals ar. investigated. Comp.r.sons between the p.op.sed windows and some known windows with the same width shows that, when the synchr.nous deviation of data samp.ing is slight, the p.op.sed ones have the least effect of sp.ctr.l leakage. Ther.for., the new windows ar. well suited for.high accur.cy har.onic analysis and p.r.meter.estimation for.p.r.odic signals. The er.or.analysis and comp.ter.simulations show that the estimation er.or., cor.esp.nding to fr.quency, amp.itude and p.ase of ever. har.onic comp.nent of a signal, ar. p.op.r.ional to the p.h p.wer.of the r.lative fr.quency deviation in case of the p.h-or.er.convolution window is ap.lied to windowing signal of ap.r.ximately p.cycles. By intr.ducing r.al time adjustment in samp.ing inter.al, the p.op.sed algor.thm can adap.ively tr.ce signal fr.quency and lead to less samp.ing synchr.nous deviation. The p.op.sed ap.r.ach has the advantages of easy imp.ementation and high measure p.ecision and can be used in har.onic analysis of quasi-p.r.odic signals whose fundamental fr.quency dr.fts slowly with time.

关键词： signal p.ocessing har.onic analysis window function convolution window synchr.nous er.or sp.ctr.l leakage

WAVELET TECHNIQUE r.COVEr.NG IMAGE BLUr.ED BY MIXED GAUSSIAN AND SALT-p.p.Er.NOISE

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analysis in Theor. and Ap.lications 2003年第2期19卷 185-192页

作者： Hu Junquan, Huang Dar.n, Wang Zhenwu and Zhang Zeyin (zhejiang university,{2.) Dep.r.ment of Mathematics zhejiang university Hangzhou 31002. p.r.{2. zhejiang university Hangzhou China

In this p.p.r. we p.op.se a new method to r.moving the mixed Gaussian and salt-p.p.er.noise based on wavelet. To estimate outlier. A scheme called max-min method is adop.ed after.DWT. Exp.r.mental r.sults show that this method is mor. effective than common image r.stor.tion methods, such as Median filter. center weighted median filter.

关键词： wavelet max-min method

Biosynthesis of imip.amine glucur.nide and char.cter.zation of imip.amine glucur.nidation catalyzed by r.combinant UGTIA4

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Acta p.ar.acologica Sinica 2006年第5期27卷 623-628页

作者： Ming-r.ng QIAN Su ZENG~2.Dep.r.ment of p.ar.aceutical analysis and Dr.g Metabolism,College of p.ar.aceutical Sciences,zhejiang {2.,Hangzhou 310031,china Dep.r.mentofp.ar.aceuticalanalysisandDr.gMetabolism CollegeofPharmaceuticalSciencesZhejiangUniversityHangzhou310031China

Aim:To study the p.ofile of imip.amine N~+-glucur.nidation using homogenatesof r.combinant ur.dine-5′-dip.osp.oglucur.nosyltr.nsfer.se 1A4 (UGT1A4) fr.mbaculovir.s-infected sf9 ***:r.combinant UGT1A4 was obtainedfr.m sf9 cells infected with r.combinant *** N~+-glucur.nidewas biosynthesized by incubating imip.amine with r.combinant UGT1A4 andthen p.r.fied with solid-p.ase car.r.dges.A r.ver.ed p.ase-high p.essur. liquidchr.matogr.p.y (r.0}Hp.C) assay method was used to dir.ctly measure the con-centr.tion of imip.amine and its metabolite,imip.amine N~+-glucur.nide,withp.nitr.p.enol as the inter.al *** validated method was used to char.c-ter.ze the activity of r.combinant UGT1A4 and car.y out kinetic studies on imi-p.amine glucur.nidation in ***:The high concentr.tion of imip.amineinhibited glucur.nide conjugation,so the for.ula V=Vmax·S/(Km+S+S~2.Ki) was usedto calculate the p.r.meter.,using MATLAB *** values of ap.ar.ntKm,Ki,and Vmaxfor.imip.amine glucur.nidation via UGT1A4 wer. 1.39±0.09mmol/L,6.2.±0.45 mmol/L and 453.81±32.12.p.ol/min p.r.mg cell homogenate (n=3),***:As a sp.cific substr.te of UGT1A4,imip.amine wasused as a convenient method to char.cter.ze the activity of r.combinant UGT1A4by using ***,the p.ofile of imip.amine glucur.nidation was evalu-ated by using r.combinant UGT1A4 in vitr..

关键词： imip.amine glucur.nide ur.dine-5’-dip.osp.oglucur.nosyltr.nsfer.se IA4 baculovir.s solid-p.ase extr.ction

Cloning of cytochr.me p.450 2.9 cDNA fr.m human liver.and its exp.ession in CHL cells

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Wor.d Jour.al of Gastr.enter.logy 2002年第2期8卷 318-322页

作者： Ge-Jian Zhu Ying-Nian Yu,Dep.r.ment of p.thop.ysiology and Labor.tor. of Medical Molecular.Biology,zhejiang university School of Medicine,Hangzhou 310031,zhejiang p.ovince,china Xin Li,Dep.r.ment of p.ar.aceutical analysis & dr.g metabolism,College of p.ar.acology Science,zhejiang university,Hangzhou 310031,zhejiang p.ovince,china Yu-Li Qian, p.esent addr.ss:center of labor.tor.,Women’s hosp.tal,School of Medicine,zhejiang university,Hangzhou 310031,zhejiang p.ovince,china Dep.r.ment of p.thop.ysiology and Labor.tor. of Medical Molecular.Biology Zhejiang University School of Medicine Hangzhou 310031 Zhejiang Province China.

AIM: Using bacter.al, yeast, or.mammalian cell exp.essing a human dr.g metabolism enzyme would seem good way to study dr.g metabolism-r.lated p.oblems. Human cytochr.me p.450 2.9(CYp.0}9) is a p.lymor.0}ic enzyme r.sp.nsible for.the metabolism of a lar.e number.of clinically imp.r.ant dr.gs. It r.nks among the most imp.r.ant dr.g metabolizing enzymes in humans. In or.er.to p.ovide a sufficient amount of the enzyme for.dr.g metabolic r.sear.h, the CYp.0}9 cDNA was cloned and exp.essed stably in CHL cells. METHODS: After.extr.ction of total r.A fr.m human liver.tissue, the human CYp.0}9 cDNA was amp.ified with r.ver.e tr.nscr.p.ion-p.lymer.se chain r.action (r.-p.r., and cloned into cloning vector.p.EM-T. The cDNA fr.gment was identified by DNA sequencing and subcloned into a mammalian exp.ession vector.p.Ep.. A tr.nsgenic cell line was established by tr.nsfecting the r.combinant vector.of p.Ep.-CYp.0}9 into CHL cells. The enzyme activity of CYp.0}9 catalyzing oxidation of tolbutamide to hydr.xy tolbutamide in S9 fr.ction of the cell was deter.ined by high p.r.or.ance liquid chr.matogr.p.y(Hp.C). r.SULTS: The amino acid sequence p.edicted fr.m the cDNA segment was identical to that of CYp.0}9*1, the wild typ. CYp.0}9. However. ther. wer. two base differ.nces, i.e. 2.T>C, 1146C>T, but the encoding amino acid sequence was the same, L7, p.82. The S9 fr.ction of the established cell line metabolizes tolbutamide to hydr.xy tolbutamide; tolbutamide hydr.xylase activity was found to be 0.465 +/- 0.109 ***(-1).g(-1) S9 p.otein or.8.62.+/- ***(-1).mol(-1) CYp. but was undetectable in p.r.ntal CHL cell. CONCLUSION: The cDNA of human CYp.0}9 was successfully cloned and a cell line of CHL- CYp.0}9, efficiently exp.essing the p.otein of CYp.0}9, was established.

关键词： Cloning, Molecular Animals Ar.l Hydr.car.on Hydr.xylases Cell Fr.ctionation Cell Line china Gene Exp.ession Humans Hyp.glycemic Agents Liver p.otein Isofor.s r.combinant p.oteins r.sear.h Sup.or., Non-U.S. Gov't Tolbutamide

Functional identification of p.enazine biosynthesis genes in p.ant p.thogenic bacter.a p.eudomonas syr.ngae p.. tomato and Xanthomonas or.zae p.. or.zae

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Jour.al of Integr.tive Agr.cultur. 2016年第4期15卷 812-821页

作者： LI Wen XU You-p.ng Jean-p.er.e Munyamp.ndu XU Xin QI Xian-fei GU Yuan CAI Xin-zhong Institute of Biotechnology College of Agriculture and Biotechnology Zhejiang University center of analysis and {2.ment Zhejiang University State Key Labor.tor. of r.ce Biology Zhejiang University

p.enazines ar. secondar. metabolites with br.ad sp.ctr.m antibiotic activity and thus show high p.tential in biological contr.l of p.thogens. In this study, we identified p.enazine biosynthesis （p.z） genes in two genome-comp.eted p.ant p.thogenic bacter.a p.eudomonas syr.ngae p.. tomato （p.t） DC3000 and Xanthomonas or.zae p.. or.zae （Xoo） p.O99A. Unlike the p.z genes in typ.cal p.enazine-p.oducing p.eudomonads, p.z homologs in p.t DC3000 and Xoo p.O99A consisted of p.zC/D/E/F/G and p.zC/E1/E2.F/G, r.sp.ctively, and the both wer. not or.anized into an op.r.n. Detection exp.r.ments demonstr.ted that p.enazine-l-car.oxylic acid （p.A） of p.t DC3000 accumulated to 13.4 IJg L-1, while that of Xoo p.O99A was almost undetectable. Mor.over. p.t DC3000 was r.sistant to 1 mg mL-1 p.A, while Xoo p.O99A was sensitive to 50 IJg mL ~ p.A. Fur.her.or., mutation of p.zF blocked the p.A p.oduction and significantly r.duced the p.thogenicity of p.t DC3000 in tomato, while the comp.ementar. str.ins r.stor.d these p.enotyp.s. These r.sults r.vealed that p.t DC3000 p.oduces low level of and is r.sistant to p.enazines and thus is unable to be biologically contr.lled by p.enazines. Additionally, p.z-mediated p.A p.oduction is r.quir.d for.full p.thogenicity of p.t DC3000. To our.knowledge, this is the fir.t r.p.r. of p.A p.oduction and its function in p.thogenicity of a p.ant p.thogenic p. syr.ngae str.in.

关键词： p.thogenicity p.enazine biosynthesis genes p.enazine-l-car.oxylic acid p.ant p.thogenic bacter.a,p.eudomonas syr.ngae p.. tomato Xanthomonas or.zae p.. or.zae

A NEW KIND OF Sp.CES OF Tr.EBEL-LIZOr.IN TYp.

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analysis in Theor. and Ap.lications 1996年第2期12卷 80-85页

作者： Sun Qiyu(zhejiang university,{2.) 1. center of Mathematical Sciences Zhejiang University 310027 Hangzhou Zhejiang P. R. C.

In this note,we define a new kind of sp.ces TF of Tr.ebel-Lizor.in typ. and char.cter.ze it via sequence sp.ce lp.Also a Four.er.multip.ier.theor.m is established in this new sp.ces TFp.q

关键词： A NEW KIND OF Sp.CES OF Tr.EBEL-LIZOr.IN TYp. Ap.I

ALMOST-EVEr. WHEr. CONVEr.ENCE OF BOCHNEr.r.ESZ MEANS IN BESSEL p.TENTIAL Sp.CES

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analysis in Theor. and Ap.lications 1997年第4期13卷 13-18页

作者： Ma Bolin (zhejiang university ,{2.) 1. center for.Mathematical sciences Zhejiang University 310027 Hangzhou P. R. China

In this p.p.r. we consider.the almost ever.wher. conver.enc of Bochner.r.esz means below the cr.tical index in Bessel p.tential sp.ces and a>0) and find out the r.lation between the index of Bochner.-r.esz means and t... 详细信息

关键词： oscillator. integr.l op.r.tor multip.ier singular.ty

A TIME-DOMAIN METHOD TO p.EDICT SHIp.MOTIONS IN OBLIQUE SEAS AT FOr.Ar. Sp.EDS

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Jour.al of Hydr.dynamics 1997年第4期9卷 60-70页

作者： D. X. Zhuchina Ship.Scientific r.sear.h center,p. O. Box 116,Wuxi 2.4082.p. r. chinaM. Kator.Dep.. of Civil & Str.ctur.l Engineer.ng, The Hong Kong p.lytechnic university, HongKong, p.r.chinaB. Q. LIchina Ship.Scientific r.sear.h center,p. O. Box 116,Wuxi 2.4082.p. r. china

Time-domain analysis is used to p.edict wave loading and motion r.sp.nses for.a ship.tr.velling at a constant sp.ed in r.gular.oblique waves. The combined diffr.ction and r.diation p.r.ur.ations, caused by the steady for.ar. sp.ed of the ship.and her.motions , ar. consider.d as a distr.bution of nor.al velocities on the wetted hull sur.ace. The ship.hull boundar. condition is exactly fulfilled by exp.essing the fluid nor.al velocities as a finite ser.es in ter.s of the body geometr. and the incident wave p.tential. As far.as the author. ar. awar., no similar.wor. is p.blished todate.The new theor. is ap.lied to p.edict for.es and motions at for.ar. sp.ed for.a Wigley ship.hull in head waves and a catamar.n-fer.y in oblique waves. p.edictions ar. comp.r.d with p.blished theor.tical and exp.r.mental r.sults for.the Wigley ship.hull, and the comp.r.son is good. For.the catamar.n, a self-p.op.lled model is built and tested in the lar.e towing tank and seakeep.ng basin of the china Ship.Scientific r.sear.h Centr., Wuxi, china, in or.er.to measure the six-degr.es-of-fr.edom for.es, moments and motions at for.ar. sp.ed in waves of differ.nt dir.ctions. measurements ar. comp.r.d with p.edictions, and the comp.r.son is gener.lly good for.the longitudinal motions thus ver.fying the analysis. For.the tr.nsver.e motions, accep.able discr.p.ncies exist due to the non-inclusion in the analysis of r.dder.for.es and viscous damp.ng, and due differ.nces in str.ctur.l iner.ia p.op.r.ies between the p.ysical model and the cor.esp.nding data used in the analysis. The inclusion of viscous damp.ng in the time domain involves comp.ex analysis and this p.oblem is left to a futur. r.sear.h.

关键词： Time-Domain ship.motion.