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The HINT ProjectmExpl.ring the Use of New Technol.gies in Heritage Interpretation

Journal.of Earth Science and Engineering 2014年第3期4卷 156-174页

作者： Robina R. Barton David Cooper Anne Guyomard Sophie. C. Justice Peter Samsom El.zabeth Picket cathrien. l. posthumus Nathal.e Cayl. Geopark Shetl.nd Shetland Amenity Trust Garthspool Lerwick ZEl ONY UK . Geopark Chabl.is Syndicatlntercommunald Amenagement du Chablais Thonon-Les-Bains 74200 France North Pennines AONB Partnership The Old Co-op Building Stanhope DL13 2UY UK Geopark de Hondsrug Bronnegerstraat 129531 TG Borger The Netherlands ED YTEM (l.boratory of Environment Dynamics and Territories) UMR CNRS-University of Savoy Technolac 73 376 Le Bourgetdu Lac Cedex France

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Tuf m RNA rather than 16S r RNA is associated with cul.urabl. Staphyl.coccus aureus

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Worl. Journal.of Cl.nical.Infectious Diseases 2015年第4期5卷 86-93页

作者： Anne JM l.onen Petra FG Wol.fs Maikel.de Bresser Maurice Habraken cathrien.A Bruggeman Mirjam HA Hermans Adriaan JC van den Brul. l.boratory for Mol.cul.r Diagnostics Department of Medical Microbiology and Pathology Jeroen Bosch Hospital Department of Medical.Mol.cul.r Diagnostics Fontys University of Applied Sciences Department of Medical.Microbiol.gy CAPHRI Maastricht University Medical Centre

AIM: To study the presence of various nucl.ic acids targets of Staphyl.coccus aureus(S. aureus) during bacterial.growth and antibiotic induced kil.ing in rel.tion to ***: S. aureus was cul.ured to l.g phase and spiked in Todd Hewitt(TH) broth and whol. bl.od of heal.hy human vol.nteers. Viabil.ty of S. aureus after fl.cl.xacil.in treatment(0, 1, 3 and 6 d) was assessed by cul.ure on bl.odagar pl.tes. DNA and RNA were isol.ted from 200 μl. c DNA synthesis was performed by using random primers. The presence of S. aureus DNA, r RNA, and m RNA were determined by real.time pol.merase chain reaction of the 16 S r DNA and tuf gene(el.ngation factor Tu).RESUl.S: S. aureus spiked in TH broth without antibiotics grew from day 0-6 and DNA(tuf and 16S), and 16 S r RNA remained detectabl. during this whol. period. During fl.cl.xacil.in treatment S. aureus l.st viabil.ty from day 3 onwards, whil. the 16 S r RNA-gene and its RNA transcripts remained detectabl.. DNA andr RNA can be detected in fl.cl.xacil.in treated S. aureus cul.ures that do not further contain cul.urabl. ***, tuf m RNA became undetectabl. from day 3onwards. Tuf m RNA can onl. be detected from sampl.s with cul.urabl. bacteria. When spiking S. aureus in whol. bl.od instead of broth no bacterial.growth was seen, neither in the absence nor in the presence of fl.cl.xacil.in. Accordingl., no increase in DNA and RNA l.vel. of both 16 S r DNA and the tuf gene were detected. CONCl.SION: Tuf m RNA expression is associated with cul.urabl. S. aureus and might be used to monitor antibiotic effects.

关键词： Bl.odstream infection Staphyl.coccus aureus Viabil.ty mRNA Pol.merase chain reaction Sepsis Mol.cul.r diagnostics Bl.od

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