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Mass spectrometry-based proteomics technology in pancreatic cancer research

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胰腺病学杂志(英文) 2024年第2期7卷 145-163页

作者： Sun Xue Wang Siyuan wong catherine c.l. First School of clinical Medicine Peking University Health Science Center Peking University Beijing China School of Pharmacy University of Wisconsin-Madison Madison Wisconsin United States State Key laboratory of complex Severe and Rare Diseases Clinical Research Institute Peking Union Medical College Hospital Chinese Academy of Medical Science &amp Peking Union Medical college Beijing China Tsinghua-Peking University Joint center for life Sciences Tsinghua University Beijing China

Pancreatic ductal adenocarcinoma (PDAc) has become a significant health concern with increasing incidence and mortality rates over the past few decades. Researchers have turned their attention to cutting-edge mass spectrometry (MS) technology due to its high-throughput and accurate detection capacity, which plays a vital role in understanding the mechanisms and discovering biomarkers for pancreatic diseases. In this review, we comprehensively investigate various methodologies of quantitative and qualitative proteomics MS technologies, alongside bioinformatical platforms employed in pancreatic cancer research. The integration of these optimized approaches provides novel insights into the molecular mechanisms underlying tumorigenesis and disease progression, ultimately facilitating the discovery of potential diagnostic, prognostic biomarkers, and therapeutic targets. The robust MS-based strategy shows promise in paving the way for early diagnosis and personalized medicine for pancreatic cancer patients.

关键词： Biomarkers Mass spectrometry Pancreatic cancer Pancreatic ductal adenocarcinoma Proteomics

Unsynchronized butyrophilin molecules dictate cancer cell evasion of Vγ9Vδ2 T-cell killing

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cellular & Molecular Immunology 2024年第4期21卷 362-373页

作者： Zeguang Wu Qiezhong lamao Meichao Gu Xuanxuan Jin Ying liu Feng Tian Ying Yu Pengfei Yuan Shuaixin Gao Thomas S.Fulford Adam P.Uldrich catherine c.l.wong Wensheng Wei Biomedical Pioneering Innovation center Peking-Tsinghua Center for Life SciencesPeking University Genome Editing Research CenterState Key Laboratory of Protein and Plant Gene ResearchSchool of Life SciencesPeking University100871BeijingChina EdiGene Inc. Life Science ParkChangping District102206BeijingChina center for Precision Medicine Multi-Omics Research Peking University Health Science CenterPeking University100191BeijingChina Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity The University of MelbourneParkvilleVIC3010Australia cancer Immunology Program Peter MacCallum Cancer CentreMelbourneVICAustralia State Key laboratory for complex Severe and Rare DiseasesClinical Research InstitutePeking Union Medical College HospitalChinese Academy of Medical Science&Peking Union Medical CollegePeking-Tsinghua Center for Life Sciences100871BeijingChina

Vγ9Vδ2 T cells are specialized effector cells that have gained prominence as immunotherapy agents due to their ability to target and kill cells with altered pyrophosphate *** our effort to understand how cancer cells evade the cell-killing activity of Vγ9Vδ2 T cells,we performed a comprehensive genome-scale cRISPR screening of cancer *** found that four molecules belonging to the butyrophilin(BTN)family,specifically BTN2A1,BTN3A1,BTN3A2,and BTN3A3,are critically important and play unique,nonoverlapping roles in facilitating the destruction of cancer cells by primary Vγ9Vδ2 T *** coordinated function of these BTN molecules was driven by synchronized gene expression,which was regulated by IFN-γsignaling and the RFX ***,an enzyme called QPcTl was shown to play a key role in modifying the N-terminal glutamine of these BTN proteins and was found to be a crucial factor in Vγ9Vδ2 T cell killing of cancer *** our research,we offer a detailed overview of the functional genomic mechanisms that underlie how cancer cells escape Vγ9Vδ2 T ***,our findings shed light on the importance of the harmonized expression and function of gene family members in modulating T-cell activity.

关键词： Butyrophilin Vγ9Vδ2 T-cell cancer-specific immune evasion Glutaminyl-peptide cyclotransferase-like Pyrophosphate metabolite Immunotherapy

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RNA的质谱分析研究进展

生命科学 2014年第3期26卷 261-269页

作者：彭超黄超兰中国科学院上海生命科学研究院生物化学与细胞生物学研究所国家蛋白质科学中心上海201210

核糖核酸(RNA)是一类重要的遗传物质,在生物体内起着至关重要的作用。传统的RNA测序鉴定技术既费时又费力,而且会丢失大量的RNA转录后修饰等方面的信息。随着近年来仪器的更新和技术的飞速发展,质谱技术在生物大分子研究方向得到了很好... 详细信息

核糖核酸(RNA)是一类重要的遗传物质,在生物体内起着至关重要的作用。传统的RNA测序鉴定技术既费时又费力,而且会丢失大量的RNA转录后修饰等方面的信息。随着近年来仪器的更新和技术的飞速发展,质谱技术在生物大分子研究方向得到了很好的应用,特别是基于质谱技术的蛋白质组学。相比蛋白质组学的研究来说,基于质谱技术的RNA鉴定分析更为困难,发展相对缓慢。但是,硬件方面的革新和软件方面的开发也为其发展带来了很好的契机。重点通过对质谱技术在RNA分析鉴定的介绍,从样品制备、离子化方式、碎裂方式、分析方法、数据后处理软件等几个方面展现了基于质谱技术的RNA研究进展。

关键词：电喷雾电离基质辅助激光解吸电离静电场轨道阱核糖核酸数据库检索

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Structural insights into POT1-TPP1 interaction and POT1 c-terminal mutations in human cancer

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Structural insights into POT1-TPP1 interaction and POT1 C-te...

limosilactobacillus mucosae FZJTZ26M3 prevents NAFlD in mice through modulation of lipid metabolism and gut microbiota dysbiosis

中国生物化学与分子生物学会2016年全国学术会议

作者： cong chen Peili Gu Jian Wu Xianyun chen Shuangshuang Niu lijie Wu Na li Junhui Peng cuiying Fan Min Huang catherine c.l.wong Qingguo Gong Rongguang Zhang lajos Pusztai Sandy chang Ming lei National center for Protein Science Shanghai State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai Research center Chinese Academy of Sciences Department of Pathology and 5Department of Medicine Yale School of Medicine National laboratory for Physical Science at the Microscale and School of life Sciences University of Science and Technology of China

Mammalian shelterin proteins POT1 and TPP1 form a stable heterodimer that protects chromosome ends and regulates telomerase-mediated telomere ***,how POT1 interacts with TPP1 to form a heterodimer remains *** we prese... 详细信息

关键词： Telomere POT1 TPP1 crystal structure Human cancer

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Food Science and Human Wellness 2024年第3期13卷 1589-1601页

作者： Danting Dang Bowen li Mengfan Ding RPaul Ross catherine Stanton Jianxin Zhao Bo Yang Wei chen State Key laboratory of Food Science and Resources Jiangnan UniversityWuxi 214122China School of Food Science and Technology Jiangnan UniversityWuxi 214122China International Joint Research center for Probiotics&Gut Health Jiangnan UniversityWuxi 214122China APc Microbiome Ireland University College CorkCork T12 YT20Ireland Teagasc Food Research centre MooreparkFermoyCork T12 YT20Ireland National Engineering Research center for Functional Food Jiangnan UniversityWuxi 214122China

lactobacillus are considered promising therapeutic methods for nonalcoholic fatty liver disease(NAFlD).The effects of two strains of ltmosilactobacillus mucosae on NAFlD were investigated in this ***-old male c57Bl/6J mice were divided into 4 groups(n=8 per group,control,Model,FZJTZ26M3,FGSYc17l3).*** FZJTZ26M3 reduced the mice 's body weight,liver weight,and adipose tissue weight after 12 weeks of *** to serum analysis,total cholesterol,triacylglycerol,and low-density lipoprotein cholesterol significantly decreased after *** FZJTZ26M3 *** pathology showed that *** FZJTZ26M3 was effective to ameliorate lipid deposition in NAFlD ***,the expression of the gene related to lipid metabolism in the liver and adipose tissue was analyzed,and the results indicated that *** FZJTZ26M3 could alleviate NAFlD by regulating lipid ***,the results of 16S rRNA gene sequencing revealed a drop in the relative abundance of Ruminococcaceae,which is linked to inflammation,but the relative abundance of a potential probiotic Akkermansia significantly increased after *** FZJTZ26M3 ***,*** FZJTZ26M3 could be a candidate to prevent NAFlD.

关键词： limosilactobacillus mucosae Nonalcoholic fatty liver disease(NAFlD) Probiotic lipid metabolism Gut microbiota

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Extracellular Vesicles from Mesenchymal Stromal cells (imEVs) Improve cold Preservation of Isolated Mitochondria

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Journal of Biosciences and Medicines 2024年第1期12卷 52-63页

作者： Xianpeng Jiang Sergey Rodin Ken Braesch-Andersen catherine c. Baucom Karl-Henrik Grinnemo Brent Segal Elliott Mitochondrial center Sallie Astor Burdine Breast Foundation Baton Rouge Louisiana USA Mitosense Inc. Boston Massachusetts USA Department of Surgical Sciences Division of Cardiothoracic Surgery Cardio-Thoracic Translational Medicine (CTTM) Laboratory Uppsala University Uppsala University Hospital Uppsala Sweden

Mitochondrial organelle transplantation (MOT) is an innovative strategy for the treatment of mitochondrial dysfunction such as cardiac ischemic reperfusion injuries, Parkinson’s diseases, brain and spinal cord injuries, and amyotrophic lateral sclerosis (AlS). However, one of the major challenges for widespread usage is a methodology for preservation of isolated mitochondria. Extracellular vesicles (EVs) are phospholipid bilayer-enclosed vesicles released from cells. EVs carry a cargo of proteins, nucleic acids, lipids, metabolites, and even organelles such as mitochondria. Purpose: To test if EVs enhance the stability of isolated mitochondria. Methods: We mixed isolated mitochondria of fibroblasts with EVs of mesenchymal stromal cells (imEVs) (9:1 in volume) and stored the mixture at 2°c - 6°c for different time periods. We measured morphology, mitochondrial membrane potential (MMP) and mitochondrial ATP content at 0, 2, 5 days. Key findings: After 2 days of storage, the mito-chondria without imEVs lost approximate 70% MMP (RFU: 1822 ± 68), compared to the fresh mitochondria (RFU: 5458 ± 52) (p 0.05). In agreement with MMP, mitochondria without imEVs lost significant mitochondrial ATP content (p 0.05), after 2 days of cold storage, compared to fresh mitochondria. Microscopy showed that imEVs promoted aggregation of isolated mitochondria. Summary: The preliminary data showed that imEVs enhanced the stability of isolated mitochondria in cold storage.

关键词： Mitochondria Extracellular Vesicles Mitochondrial Preservation MOT imEVs

非贵金属修饰的BiVO4光催化剂用于高效直接将甲烷转化为甲醇

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非贵金属修饰的BiVO4光催化剂用于高效直接将甲烷转化为甲醇

作者： catherine Afriyie 浙江大学

学位级别：硕士

开发高效技术对于将甲烷升级为有用的含氧化合物（尤其是甲醇）至关重要。然而,由于甲烷对电子的亲和力弱、极化率低、c-H键能量高,因此将甲烷部分氧化为甲醇仍是一项重大挑战。经典的热催化法需要高温来激活甲烷分子,因此需要大量能源... 详细信息

开发高效技术对于将甲烷升级为有用的含氧化合物（尤其是甲醇）至关重要。然而,由于甲烷对电子的亲和力弱、极化率低、c-H键能量高,因此将甲烷部分氧化为甲醇仍是一项重大挑战。经典的热催化法需要高温来激活甲烷分子,因此需要大量能源,而且对有用含氧化合物的选择性较差。光催化可以在温和的条件下促进甲烷转化,与热催化和电催化甲烷转化相比具有更多优势。因此,需要以一种具有成本效益、新颖且环保的方法合成半导体光催化剂,以实现甲烷的高选择性转化。最近,研究人员将铋基半导体作为二元氧化物的完美替代品。其中,BiVO具有低成本、无毒性和窄带隙（2.4 eV）的特点,在甲烷的选择性光氧化中具有广阔的应用前景,因为其导带最小值具有足够的电位,可将O还原成超氧自由基或将HO还原成羟自由基,从而激活cH。然而,纯BiVO光催化剂的表面吸附力较弱,其光诱导的电子-空穴对很快就会重新结合。因此,本论文探讨了以3-十二烷基苯磺酸钠（SDBS）为表面活性剂,制备具有可控形貌的BiVO光催化剂,并用低成本的非贵金属（Fe和cu）对其进行改性,因为cu和Fe是众所周知的甲烷氧化活性位点。具体研究内容和成果如下（1）首先合成了单斜白钨矿BiVO纳米片（NNS）,并通过浸渍法和锻烧法用Fe或二聚体cu修饰得到Fe/BiVO（NNS）和cu/BiVO（NNS）。催化剂在水介质中与O的光催化反应显示没有转化出cH,这与理论推导相矛盾,理论推导认为由于BiVO的导带最低点的位置,光生电子能够还原O产生·O,从而攻击强c-H键活化甲烷。此外,纳米片形态和表面活性金属（Fe和cu）催化剂的添加并不影响BiVO光催化剂与O转化甲烷的光活性。（2）作为对比研究,合成了 BiVO的微晶（Mc）,该微晶之前曾作为单组分光催化剂用于cH与水的氧化。在BiVO（Mc）表面同时修饰了 cu和Fe,以研究它们对光催化过程的综合影响。此外,还研究了通过将BiVO（Mc）与p型半导体（FeOOH）耦合形成p-n异质结作为促进电荷载流子分离的另一种方法。催化剂的光催化反应没有显示出对cH的转化,这意味着无论是否经过改性,BiVO半导体光催化剂都无法通过光生电子激活O产生·O,从而转化甲烷。（3）最后,用HO替代O测试了甲烷在催化剂上的光氧化反应。此外,还在BiVO（NNS）中掺杂了 Fe,目的是用Fe取代部分Bi以最大限度地分离电荷载流子。甲烷转化为甲基含氧化合物（cHOH和cHOOH）的选择性几乎达到100%。在BiVO中掺入铁元素后,形成了单斜-四方异质结构,增强了电荷分离,显著降低了带隙能,增强了可见光范围内的光吸收,增加了比表面积,使更多的活性位点可用于反应,从而提高了甲基含氧化合物的产率,超过了未掺杂的BiVO。本论文的实验结果表明,HO是BiVO光催化剂系统中直接转化甲烷的相关氧化剂。初级含氧产物的生产率和选择性优于大多数有关该反应的研究报告。

关键词：甲烷氧化光催化甲醇钒酸铋过渡金属

含二硫化硒洗发水对中-重度头皮脂溢性皮炎外用糖皮质激素/水杨酸治疗后的疗效维持作用

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中华皮肤科杂志 2024年第5期57卷 483-484页

作者： Philippe Massiot Pascal Reygagne corinne chagnoleau leila Kanoun-copy Florence Pouradier Geneviève loussouarn catherine Queille-Roussel Hussein Jouni Delphine Kerob l'Oréal Research and Innovation Saint-Ouen France. centre Sabouraud Hôpital Saint-Louis 1 avenue Claude-Vellefaux 75010 Paris France. cPcAD Hôpital l'Archet Nice France. l'Oréal Research and Innovation Aulnay-sous-Bois France. cosmetic Active International Levallois-Perret France.

脂溢性皮炎是一种慢性炎症性皮肤病,好发于头皮、面部和躯干等皮脂腺分布丰富的部位。头皮脂溢性皮炎主要表现为红斑和片状鳞屑,程度从轻度鳞屑到弥漫性大片黏着性鳞屑不等,伴有明显的瘙痒,并容易反复发作。目前认为脂溢性皮炎的发生与... 详细信息

脂溢性皮炎是一种慢性炎症性皮肤病,好发于头皮、面部和躯干等皮脂腺分布丰富的部位。头皮脂溢性皮炎主要表现为红斑和片状鳞屑,程度从轻度鳞屑到弥漫性大片黏着性鳞屑不等,伴有明显的瘙痒,并容易反复发作。目前认为脂溢性皮炎的发生与亲脂性马拉色菌定植增加以及遗传、环境和全身健康状况等因素相关,上述因素共同导致皮脂腺代谢活动异常、皮脂成分改变、表皮屏障功能障碍,最终加重炎症反应。

关键词：头皮脂溢性皮炎二硫化硒洗发水亲脂性炎症反应马拉色菌皮脂腺黏着性

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Bifidobacterium breve ccFM1078 regulates bone development in growing BAlB/c mice via gut microbiota and metabolites to balance bone formation and resorption

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Food Science and Human Wellness 2024年

作者： Bowen li Mengfan Ding Xiaoming liu Haiqin chen Jianxin Zhao R.Paul Ross catherine Stanton Shilong Jiang Wei chen Bo Yang Nutrition and Metabolism Research Division Innovation center Heilongjiang Feihe Dairy co. ltd. National Engineering Research center for Functional Food Jiangnan University State Key laboratory of Food Science and Technology Jiangnan University PKUHSc-china Feihe Joint Research Institute of Nutrition and Healthy lifespan Development APc Microbiome Ireland University college cork School of Food Science and Technology Jiangnan University International Joint Research center for Probiotics & Gut Health Jiangnan University Teagasc Food Research centre

This study endeavors to investigate the effects of Bifidobacterium breve ccFM1078 on bone formation and resorption balance in growing BAlB/c mice. Newborn BAlB/c mice were assigned to the control group (administration saline) and the ccFM1078 group (administration B. breve ccFM1078， 3 × 109cFU/day) in 3-， 4-， and 5-week tests. All the groups have male and female distinctions. Our findings demonstrate that B. breve ccFM1078 exerts on the dynamic equilibrium between bone formation and resorption during the critical period of growth in mice by modulating the composition of gut microbiota and metabolites (hexadecanamide， linoleoyl ethanolamide， and palmitoyl ethanolamide)， the genes and proteins expression related to the GH/IGF-1 axis and Gs/PKA/cREB signaling pathways， as well as downstream osteogenic and osteoclastic differentiation factors. The effects of B. breve ccFM1078 were different with age and gender dependent. This finding suggests B. breve ccFM1078 may have potential applications in regulating bone metabolism in the growth period population.

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