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Antifungal Activity of Oleoresin and Fractions of Pinus elliottii Engelm and Pinus tropicalis against Phytopathogens

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American Journal of Plant Sciences 2014年第26期5卷 3898-3903页

作者： Gessica Andrade Fariza Abrao Patrick Silva Sergio Ricardo Ambrosio Rodrigo cassio Sola Veneziani Wilson Roberto Cunha Regina Helena Pires Carlos Henrique G.Martins Laboratory of Research in Applied Microbiology University of FrancaFrancaBrazil Research Group on Natural Products University of FrancaFrancaBrazil

Microorganism resistance to the existing products is yet another difficulty that agriculturalists have to deal with. In this context, the search for new agricultural products that can fight phytopathogens has become increasingly important. Plants have played an important role in this process, because they can serve as a source of new compounds for drug discovery. Plants belonging to the genus Pinus produce an oleoresin that protects the plant against herbivores and pathogens. With a view to developing products that can combat fungal pathogens without harming the environment, this work aimed to evaluate the antifungal activity of the oleoresins and fractions of Pinus elliottii Engelm and Pinus tropicalis against phytopathogens. The methodology based on NCCLS M38-A standards aided antifungal activity assessment. The microdilution method helped to determine the Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC). The oleoresins of P. elliottii and P. tropicalis afforded the most significant results—they displayed fungicidal activity against all the tested species. MIC values were promising, especially the MIC of the oleoresin of P. elliottii against S. rolfsii (1.95 μg·mL-1). The MIC values of the oleoresins of P. elliottii and P. tropicalis ranged from 1.95 to 1000 μg·mL-1 and from 31.25 to 250 μg·mL-1, respectively. Fraction PT2 of P. tropicalis furnished the best results among all the assayed fractions: MIC values lay between 125 and 500 μg·mL-11. In conclusion, the oleoresin of P. tropicalis is a promising source of new antifungal agents for application in the treatment of phytopathogenic infections.

关键词： Pinus elliottii Pinus tropicalis Phytopathogens Antifungal Activity Oleoresin

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Analysis of the Tropism and Anti-Bacterial Potential of a Type of Clay

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Open Journal of Medical Microbiology 2014年第2期4卷 140-145页

作者： cassio Ilan Soares Medeiros Erica Renata Nogueira Sa Juliana Raquel de Morais Santos Oliveira Lis Magalhaes Geraldo Goncalves de Almeida Filho Hilzeth de Luna Freire Pessoa Graduate Student in Biomedicine Integrated College of PatosParaibaBrazil ICU Specialist Physiotherapist Integrated College of PatosParaibaBrazil Medical Student College of Santa MariaCajazeiras ParaibaBrazil MSc.in Natural and Synthetic Bioactive Products Federal University of ParaibaUFPBJoao PessoaBrazil

Natural clays have been used by man in infections of bacterial etiology, since the first historical registers. Our attention turned to a red-colored clay, known in the northeast of Brazil as “barro de louça” (dish clay). These clays and other natural earth materials seem interesting to us, as the blockage of the liberation of toxins or inactivation, may be related to the interruption of infection cycles in the skin and mucous membranes. The adsorptive and absorptive properties of the mineral clays are well documented in the cure process of skin and gastrointestinal diseases. Susceptibility and bacterial tropism tests were carried out. The results were analyzed and interpreted according to the conventional microbiological protocol. The bacterial strains, Staphylococcus aureus, Escherichia coli e Pseudomonas aeruginosa, did not present a susceptibility profile to an isotonic solution of clay, but there was an increase of the bacterial tropism as the concentration of the isotonic solution was increased, being the minimal observed concentration of 100 mg/mL. Our aim is to document a type of red clay from the northeast of Brazil with possible attraction properties (Tropism) to bacteria and their toxins.

关键词： Red Clay Bacterial Tropism Bacterial Resistance

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Different Approaches on the Investigation of Ground Water

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Journal of Earth Science and Engineering 2018年第1期8卷 39-49页

作者： cassio Stein Moura Roberto Heemann Moises Razeira Daniela Govoni Sotelo Gabriela Borges Soares Giovanna Ramos Garcez Jfilio Cesar Gall Pires Vanessa da Conceicao Osorio Heldiane Souza dos Santos Institute of Petroleum and Natural Resources Pontifical University of Rio Grande do Sul Porto Alegre 90619-900 Brazil Federal University of the Pampa Cacapava do Sul 96570-000 Av. lpiranga 6681 Predio 96 J Porto Alegre RS Brazil

In this paper we discuss ways to obtain information about the quality of ground water and their availability. We classify the different approaches in two categories： geophysical methods, e.g., electroresistivity sounding, seismic survey, gravimetry, MT （magnetotelluric） method, and geochemical methods. The former ones are able to provide information on the geological structure,meaning depth, range, amount of water and possible connections among different exploration areas or regions at risk due to contamination. On the other hand, the last ones provide information about the quality of water and the possible of use for agriculture, industry or human consumption. As a case study we aim at the Guarani Aquifer, more specifically at its recharge zone on the southern rim.

关键词： Drought ground water geophysics geochemistry.

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Use of the American Society of Anesthesiologists Classification as an Additional Planning Tool for Renal Cell Carcinoma Assessment

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Journal of Cancer Therapy 2013年第9期4卷 7-14页

作者： Daniel Beltrame Ferreira Stenio de cassio Zequi Walter Henriques da Costa Diego Abreu Clavijo Ricardo Decia Deusdedit Cortez Neto Renato Rosa de Oliveira Gustavo Cardoso Guimaraes Ademar Lopes Urology Division Department of Pelvic SurgeryA.C.Camargo Cancer CenterSao PauloBrazil Department of Urology Pasteur HospitalMontevideoUruguay

Objective: To determine the prognostic value of the American Society of Anesthesiologists (ASA) classification and of the main clinical pathologic variables in renal cell carcinoma (RCC) patients who underwent surgical treatment. Methods: In this international collaborative study, 376 RCC patients who underwent partial nephrectomy (PN) or radical nephrectomy (RN) during the period 1989-2009 were assessed. The pathological data were reviewed by a single pathologist, and all of the surgically treated patients had been previously evaluated by a team of anesthesiologists and classified as ASA 1, ASA 2, or ASA 3. Results: In total, 318 patients were included in the study, with a mean followup of 48 months. Incidental tumors represented 47% of the cases, while 11.6% presented with metastases at diagnosis. Among the patients assessed, 38 (11.9%) were classified as ASA 1, 213 (67%) as ASA 2, and 67 (21.1%) as ASA 3. An association between the ASA classification and the main clinicopathological variables of RCC was observed. The univariate analysis for overall survival (OS) revealed significant differences in the survival curves according to the ASA classification (p < 0.001). High-grade neoplasms, the presence of metastasis at diagnosis, clinical stage III/IV, and incidental tumors remained as independent predictors of survival. Moreover, the multivariate analysis revealed a negative impact of the ASA classification on OS (p = 0.001). Conclusions: The present study demonstrated a correlation between the ASA classification and the main prognostic factors of RCC and its impact on survival rates. ASA 3 patients had more aggressive tumors, increased risk of perioperative complications, and worse outcomes compared with ASA1 or ASA 2. Thus, the ASA classification may be considered an additional tool for assessing and planning the treatment of RCC patients.

关键词： Comorbidity Renal Cell Carcinoma ASA Prognosis Oncology

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Relationship between vascular endothelium and periodontal disease in atherosclerotic lesions: Review article

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World Journal of Cardiology 2015年第1期7卷 26-30页

作者： Marco Aurélio Lumertz Saffi Mariana Vargas Furtado Carisi Anne Polanczyk Márlon Munhoz Montenegro Ingrid Webb Josephson Ribeiro cassio Kampits Alex Nogueira Haas Cassiano Kuchenbecker Rosing Eneida Rejane Rabelo-Silva Cardiovascular Division Hospital de Clínicas de Porto Alegre Porto Alegre 90035-003 Brazil Federal University of Rio Grande do Sul Faculty of Dentistry Periodontology Porto Alegre 90030-035 Brazil School of Nursing Federal University of Rio Grande do Sul Porto Alegre 90620-110 Brazil

Inflammation and endothelial dysfunction are linked to the pathogenesis of atherosclerotic disease. Recent studies suggest that periodontal infection and the ensuing increase in the levels of inflammatory markers may be associated with myocardial infarction, peripheral vascular disease and cerebrovascular disease. The present article aimed at reviewing contemporary data on the pathophysiology of vascular endothelium and its association with periodontitis in the scenario of cardiovascular disease.

关键词： Endothelium Vascular Atherosclerosis Periodontal diseases Nitric oxide Cardiovascular diseases

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Parallel Implementation of the BiCGStab（2） Method in GPU Using CUDA and Matlab for Solution of Linear Systems

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通讯和计算机（中英文版） 2014年第4期11卷 339-346页

作者： Lauro cassio Martins de Paula Anderson da Silva Soares Institute of lnformatics Federal University of Goias (UFG) Goias 74001-970 Brazil

This paper presents a parallel implementation of the hybrid BiCGStab(2) (bi-conjugate gradient stabilized) iterative method in a GPU (graphics processing unit) for solution of large and sparse linear systems. This implementation uses the CUDA-Matlab integration, in which the method operations are performed in a GPU core using Matlab built-in functions. The goal is to show that the exploitation of parallelism by using this new technology can provide a significant computational performance. For the validation of the work, we compared the proposed implementation with a BiCGStab(2) sequential and parallelized implementation in the C and CUDA-C languages. The results showed that the proposed implementation is more efficient and can be viable for simulations being carried out with quality and in a timely manner. The gains in computational efficiency were 76x and 6x compared to the implementation in C and CUDA-C, respectively.

关键词： Matlab 并行实现线性系统 GPU 图形处理单元双共轭梯度内置函数计算性能

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Thyroid Nodule: Alpha Score 2.0 Classification for FNAB Selection, Multicentric Study in Latin America

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Open Journal of Radiology 2021年第4期11卷 160-174页

作者： Glenn Mena Maria Cristina Chammas Carlos Mario Gonzalez Vasquez Lylian Rocío Villagómez Marco Alfredo Muñoz Pico Patricio Alejandro Montalvo Santiago Mena-Bucheli Julio Olmedo Elizabeth Quintero Pedro Henrique de Marqui Moraes Osmar cassio Saito Hubertino Diaz Denise Romero Gabriela Velalcazar Angel Ramón Sosa Fleitas Yamil Oliver Quevedo Ontaneda Victor Ricardo Chara Alpha Imagen Radiología e Intervencionismo Quito Ecuador Hospital das Clínicas Faculdade de Medicina da Universidade de Sã o Paulo S&#227 o Paulo Brasil Hospital Pablo Tobón Uribe Medellin Colombia Hospital del Instituto Ecuatoriano de Seguridad Social Quito Sur Quito Ecuador Hospital San Francisco de Quito Quito Ecuador Hospital Edgardo Rebagliati Martins Lima Perú Hospital Carlos Andrade Marín Quito Ecuador Hospital de Clínicas San José de San Martín Buenos Aires Argentina Hospital Universitario de Los Andes Mérida Venezuela Hospital de la Policía Quito Ecuador Hospital Sisol Tacna Perú

Introduction: To perform a Latin-American multicentric study for the prediction of benign and malignant thyroid nodules using Alpha Score, and to compare it with ACR TIRADS® and Bethesda®. Materials and Methods: A prospective multicentric study in 10 radiological hospitals and institutions of Latin America was performed and 818 thyroid nodules were analyzed by ultrasound and classified by using both ACR TIRADS® and Alpha Score;fine-needle aspiration biopsy was performed when needed and classified with Bethesda. The relationships between predictors were analyzed by using binary logistic regression, statistical significance was defined by a p-value of 0.05, with an error margin of 4% and 95% confidence intervals. Results: Alpha Score 2.0 establishes five types of malignant predictors: microcalcifications, irregular borders, taller-than-wide shape, predominant solid texture and hypoechogenicity;a diameter equal to or greater than 1.5 cm adds an extra point to the final score. Resulting classification divides TNs into 4 categories: benign (1.9%), low suspicion (8.7%), mild suspicion (13.6%) and high suspicion (75.7%) of malignancy probability;sensitivity of 82%, specificity of 74%, the positive predictive value of 94%, the negative predictive value of 51%, the statistical accuracy of 81%, odds ratio of 108.89 and correlation with ACR TIRADS of 0.77 and Bethesda of 0.91. Conclusions: Alpha Score 2.0 has superior diagnostic accuracy and performance compared to the previously published Alpha Score and is able to classify a benign TN in a precise, safe and accurate way, avoiding unnecessary FNABs or determining the necessity of FNAB in cases of moderate to high suspicion of malignancy.

关键词： Thyroid Cancer-Clinical Radiology-Imaging Thyroid Nodule Alpha Score TIRADS® Thyroid Ultrasonography

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系统性红斑狼疮

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美国医学会杂志（中文版） 2005年第5期24卷 320-320页

作者： Sarah Ringold cassio Lynm 顾佳（译）

系统性红班狼疮（systemic lupus erythematosus，SLE）也叫狼疮，是一种自身免疫性疾病，此时人体内的免疫系统（体内抗感染细胞）异常攻击机体自身的组织器官，诱发炎症和损伤。狼疮多影响育龄妇女，也可见于儿童、青少年和男性。狼... 详细信息

系统性红班狼疮（systemic lupus erythematosus，SLE）也叫狼疮，是一种自身免疫性疾病，此时人体内的免疫系统（体内抗感染细胞）异常攻击机体自身的组织器官，诱发炎症和损伤。狼疮多影响育龄妇女，也可见于儿童、青少年和男性。狼疮的病因尚不清楚，可能与遗传因素、环境因素和感染有关。该病可影响人体几乎所有器官，肾脏受累最为常见。有些狼疮病例病情轻微（比如仅累及皮肤），而另一些病例病情极为严重（影响包括大脑在内的多个器官）。病程具有发作（活动期）和缓解（非活动期）的特点。2005年6月22日-29日出版的JAMA杂志中含有一篇文章，阐述了狼疮性肾病的现有治疗措施。

关键词：系统性红斑狼疮 erythematosus 自身免疫性疾病系统性红班狼疮组织器官感染细胞 lupus 狼疮性肾病免疫系统育龄妇女

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肾移植

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美国医学会杂志（中文版） 2006年第3期25卷 192-192页

作者： Janet M Torpy cassio Lynm（Illustrator）顾佳（译）

肾脏可以滤过血液，排泄废物，分泌激素，产生尿液。尿液由双肾经输尿管引流到膀胱中，并储存于此。对于终末期肾病（又称作慢性肾功能衰竭）患者而言，肾透析（采用仪器来替代肾脏，排出废物）或肾移植不失为治疗之选。移植成功的肾脏... 详细信息

肾脏可以滤过血液，排泄废物，分泌激素，产生尿液。尿液由双肾经输尿管引流到膀胱中，并储存于此。对于终末期肾病（又称作慢性肾功能衰竭）患者而言，肾透析（采用仪器来替代肾脏，排出废物）或肾移植不失为治疗之选。移植成功的肾脏能像自身健康的肾脏一样发挥作用。这意味着患者不再需要进行透析，也不需要服用某些药物。当然，并非所有肾功能衰竭患者均适于行肾移植，在选择治疗方案时应与医生进行商讨。2005年12月7日出版的JAMA杂志中含有一篇有关肾移植的文章。

关键词：肾移植肾功能衰竭患者慢性肾功能衰竭治疗方案终末期肾病分泌激素自身健康 JAMA 肾透析肾脏

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食用水产品的利弊

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美国医学会杂志（中文版） 2007年第5期26卷 320-320页

作者： Janet M.Torpy cassio Lynm Richard M. Glass 顾佳（译）

饮食可影响人体健康。水产品（无论是鱼还是贝）除含有蛋白质、维生素D及硒等营养成分外，还具有一类特殊的脂肪，即ε-3脂肪酸，后者可减少心脏及其他疾病的患病风险。但是，水产品中有可能还含有汞及其他污染物，对人体健康构成威胁。... 详细信息

饮食可影响人体健康。水产品（无论是鱼还是贝）除含有蛋白质、维生素D及硒等营养成分外，还具有一类特殊的脂肪，即ε-3脂肪酸，后者可减少心脏及其他疾病的患病风险。但是，水产品中有可能还含有汞及其他污染物，对人体健康构成威胁。在2006年10月18日刊出的JAMA杂志中，有一篇文章阐述了食用水产品的利与弊。

关键词：水产品食用人体健康营养成分维生素D 患病风险 JAMA 脂肪酸

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