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Optimization of high throughput spectral flow cytometry for immune cell profiling in mouse liver

Liver Research 2023年第3期7卷 263-271页

作者： Grayson W.Way Hongkun Lu Xuan Wang Derrick Zhao carmen camarena Devanand Sarkar Rebecca K.Martin Huiping Zhou C.Kenneth and Dianne Wright Center for Clinical and Translational Research Virginia Commonwealth UniversityRichmondVAUSA Department of Microbiology and Immunology Virginia Commonwealth University School of MedicineRichmondVAUSA Department of Human and Molecular Genetics Massey Cancer CenterVCU Institute of Molecular Medicine(VIMM)Virginia Commonwealth UniversityRichmondVAUSA Massey Cancer Center Virginia Commonwealth UniversityRichmondVAUSA VCU Institute of Molecular Medicine Virginia Commonwealth UniversityRichmondVAUSA Central Virginia Veterans Healthcare System RichmondVAUSA

The liver plays an important role in both metabolism and *** of the hepatic immune microenvironment is closely associated with various liver *** gain a better understanding of how different types of immune cells contribute to the progression of liver diseases,it is crucial to thoroughly characterize hepatic immune *** direct digestion of liver tissue is a relatively simple method for isolating immune cells,it often induces excessive hepatocyte death,which causes a release of intracellular components that leads to the activation of stress responses and injury in the surrounding *** injury can lead to excessive death in the hepatic immune cells,making isolation and accurate characterization of the immune profile challenging,especially in diseased *** method described here addresses these challenges by utilizing Phosphate buffered saline(PBS)and digestion buffer perfusions to eliminate contaminating blood cells,ensure a pure hepatic immune population,and minimize hepatic immune cell *** ex vivo digestion of the liver enables the isolation of the immune cells from the hepatic tissues and the generation of a single-cell suspension that can be stained for spectral flow *** enhance intracellular cytokine detection and maintain signaling under different physiological and pathological conditions,this protocol uses an in vivo administration of Brefeldin A,a less toxic inhibitor of cytokine *** in vivo administration of Brefeldin A allows for a more accurate representation of the immune cell function and cytokine expression compared to the traditionally used ex vivo Brefeldin A administration.A comprehensive spectral flow cytometry panel,comprising extracellular and intracellular staining,is used for deep immunophenotyping and immune cell effector function *** this protocol is specifically designed for liver digestion of Mdr2 knockout mice(a model for primary sclerosing cholangitis)and f

关键词： metabolism flow utilizing

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Antibacterial activity of five Peruvian medicinal plants against Pseudomonas aeruginosa

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Asian Pacific Journal of Tropical Biomedicine 2015年第11期5卷 928-931页

作者： Gabriela Ulloa-Urizar Miguel Angel Aguilar-Luis María del carmen De Lama-Odría José camarena-Lizarzaburu Juana del Valle Mendoza Centro de Investigacin de la Facultad de Ciencias de la Salud Universidad Peruana de Ciencias Aplicadas-UPC Facultad de Ciencias Biolgicas Universidad Nacional Mayor de San Marcos (UNMSM) Instituto de Investigacin Nutricional

Objective: To evaluate the susceptibility of Pseudomonas aeruginosa(P. aeruginosa)in vitro to the ethanolic extracts obtained from five different Peruvian medicinal ***: The plants were chopped and soaked in absolute ethanol(1:2, w/v). The antibacterial activity of compounds against P. aeruginosa was evaluated using the cupplate agar diffusion ***: The extracts from Maytenus macrocarpa("Chuchuhuasi"), Dracontium loretense Krause("Jergon Sacha"), Tabebuia impetiginosa("Tahuari"), Eucalyptus camaldulensis Dehn(eucalyptus), Uncaria tomentosa("U?a de gato") exhibited favorable antibacterial activity against P. aeruginosa. The inhibitory effect of the extracts on the strains of P. aeruginosa tested demonstrated that Tabebuia impetiginosa and Maytenus macrocarpa possess higher antibacterial ***: The results of the present study scientifically validate the inhibitory capacity of the five medicinal plants attributed by their common use in folk medicine and contribute towards the development of new treatment options based on natural products.

关键词： Pseudomonas Medicinal plants Maytenus macrocarpa D

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