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Selection and Analysis of Polymorphisms in Somaclonal Variants of Agave americana Resistant to Fusarium oxysporum via an Ethyl methanesulphonate Treatment

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Phyton-International Journal of Experimental Botany 2021年第6期90卷 1727-1739页

作者： Sheila Jazmín Reyes-Zambrano carlos Alberto Lecona-guzmán Federico Antonio Gutiérrez-miceli Víctor manuel Ruiz-Valdiviezo Arely Anayansi Vargas-Díaz Laboratorio de Biotecnología Vegetal Instituto Tecnológico de Tuxtla GutiérrezTecnológico Nacional de MéxicoTuxtla GutiérrezChiapas29050México ConACYT-Colegio de Postgraduados Campus Campeche ChampotónCampeche24450México

Agave americana *** were exposed to different concentrations of ethyl methanesulphonate(EmS)0,15,30,45 and 60 mm and to different times of exposure(2 and 4 h).The viability and capacity of shoot formation were shown to be affected when the callus were exposed to high concentrations(30–60 mm).only the callus exposed to 15 mm EmS presented shoot formation;the exposure time of two hours produced the largest quantity of shoots regenerated per callus(21 shoots/callus).In order to generate somaclonal variants resistant to Fusarium oxysporum,a selection pressure was applied through of a culture filtrate(CF)of 100 ppm of the *** was made in callus obtained in the treatment with 15 mm EmS during 2 h of *** CF caused oxidation and necrosis in 71.25%of the callus;however,they were capable of generating shoots(3.5 shoots/callus).molecular markers type RAPD,ISSR and DAmD were used to evaluate the genetic variation arising from the mutations caused by EmS on control plants and 16-month-old somaclonal *** polymorphic information content(PIC)for each one of the initiating groups was:0.28(DAmD),0.09(ISSR)and 0.14(RAPD).DAmD revealed a greater percentage of polymorphism than RAPD and *** bands were detected in the somaclonal *** indicated that the EmS caused genetic variation in the regenerated plants conferring resistance to them against Fusarium oxysporum.

关键词： mutagenesis polymorphisms genetic variation molecular markers

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Discriminatory capacity of serum interleukin-6 between complicated and uncomplicated acute appendicitis in children:a prospective validation study

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World Journal of Pediatrics 2022年第12期18卷 810-817页

作者： Javier Arredondo montero Giuseppa Antona Adriana Rivero marcotegui carlos BardajíPascual mónica Bronte Anaut Raquel Ros Briones Amaya Fernández-Celis natalia López-Andrés nerea martín-Calvo Pediatric Surgery Department Hospital Universitario de NavarraIrunlarrea 331008PamplonaSpain Clinical Analysis Department Hospital Universitario de NavarraPamplonaSpain Pathology Department Hospital Universitario de NavarraPamplonaSpain Cardiovascular Translational Research NavarraBiomed(Miguel Servet Foundation)Hospital Universitario de NavarraUniversidad Pública de Navarra(UPNA)IdiSNAPamplonaSpain School of medicine Department of Preventive Medicine and Public HealthUniversity of NavarraPamplonaSpain CIBER Fisiopatología de la obesidad y nutrición(CIBERobn) Health Institute Carlos IIIMadridSpain Instituto de Investigación Sanitaria de navarra IdiSNAPamplonaNavarraSpain

Background Serum interleukin-6(IL-6)has a moderate diagnostic performance in pediatric acute appendicitis(PAA).The evidence regarding its capacity to discern between complicated and uncomplicated PAA is *** We designed a prospective observational study to validate serum IL-6 as a marker for diagnostic classification between complicated and uncomplicated *** study included 205 patients divided into three groups:(1)patients who underwent major outpatient surgery(n=57);(2)patients with non-surgical abdominal pain(nSAP)in whom the diagnosis of PAA was excluded(n=53),and(3)patients with a confirmed diagnosis of PAA(n=95).The PAA patients were further classified as uncomplicated or complicated ***-6 concentration was determined in all patients at *** statistical analysis was performed using the mann-Whitney U test,the Fisher exact test and the Kruskall Wallis *** area under the receiver operating characteristic curves(AUC)were *** median(interquartile range,IQR)serum IL-6 values were 2 pg/mL(2.0–3.4)in group 1,3.9 pg/mL(2.4–11.9)in group 2,and 23.9 pg/mL(11.1–61.0)in group 3(Pmong the participants in group 3,those with uncomplicated PAA had median(IQR)serum IL-6 values of 17.2 pg/mL(8.5–36.8),and those with complicated PAA had 60.25 pg/mL(27.1–169)serum IL-6(Poff point of 19.55 pg/mL,the AUC for the discrimination between patients in group 2 vs.3 was 0.83[95%confidence interval(CI)0.76–0.90],with a sensitivity of 61.3%and a specificity of *** AUC for the discrimination between patients with uncomplicated and complicated PAA was 0.77(95%CI 0.68–0.86)and the cut-off point was 25.90 pg/mL,with a sensitivity and specificity of 84.6%and 65.6%,*** Serum IL-6 has a good performance in discerning between complicated and uncomplicated PAA.A score including clinical and radiological variables may increase the diagnostic performance of this molecule.

关键词： Acute Appendicitis Complicated Diagnostic Interleukin-6 Pediatric Uncomplicated

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Auricular embryonal rhabdomyosarcoma

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World Journal of Pediatrics 2022年第5期18卷 358-360页

作者： Javier Arredondo montero monica Bronte Anaut Sara Hernandez-martin Juan carlos Lopez-Gutierrez Pediatric Surgery Department Complejo Hospitalario de NavarraCalle Irunlarrea 331008 PamplonaNavarraSpain Pathology Department Complejo Hospitalario de NavarraPamplonaSpain Pediatric Surgery Department Hospital Universitario La PazMadridSpain

A 13-month-old girl presented with a red-violaceous tumor of 13 mm in diameter on the inner side of the root of the right helix,with increased temperature and cystic consistency(Fig.1).no pulse was palpable in the *** ultrasonography was performed,showing a 1.6 cm rounded lesion with well-defined margins,hypoechogenic and homogeneous,compatible with *** treatment with oral propranolol was started without improve-ment.

关键词： sarcoma ultrasonography showing

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Latest developments in early diagnosis and specific treatment of severe influenza infection

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Journal of Intensive medicine 2024年第2期4卷 160-174页

作者： Francisco Valenzuela-Sánchez Blanca Valenzuela-méndez Juan Francisco Rodríguez-Gutiérrez Ángel Estella Intensive Care Unit University Hospital of JerezRonda de Circunvalación s/nJerez de la FronteraSpain Haematology Department University Hospital of JerezRonda de Circunvalación s/nJerez de la FronteraSpain Centro de Investigación Biomédica en Red Enfermedades respiratoriasCIBERESInstituto de Salud Carlos IIIAv.de Monforte de LemosMadridSpain Department of oncological Surgery Institut du Cancer de Montpellier(ICM)Parc Euromédecine208 Av.des ApothicairesMontpellierFrance Department of medicine Faculty of MedicineUniversity of CádizCalle Doctor MarañónCádizSpain Instituto de Investigación e Innovación Biomédica de Cádiz(InIBiCA) Avenida Ana de Viya 21CádizSpain

Influenza pandemics are unpredictable recurrent events with global health,economic,and social *** objective of this review is to provide an update on the latest developments in early diagnosis and specific treatment of the disease and its complications,particularly with regard to respiratory organ *** advances in treatment,the rate of mortality in the intensive care unit remains approximately 30%.Therefore,early identification of potentially severe viral pneumonia is extremely important to optimize treatment in these *** pathogenesis of influenza virus infection depends on viral virulence and host ***,in some patients,it is associated with an excessive systemic response mediated by an authentic cytokine *** process leads to severe primary pneumonia and acute respiratory distress *** prognostication in the emergency department based on comorbidities,vital signs,and biomarkers(e.g.,procalcitonin,ferritin,human leukocyte antigen-DR,mid-regional proadrenomedullin,and lactate)is *** of these biomarkers on admission may facilitate clinical decision-making to determine early admission to the hospital or the intensive care *** decisions are reached considering pathophysiological circumstances that are associated with a poor prognosis(e.g.,bacterial co-infection,hyperinflammation,immune paralysis,severe endothelial damage,organ dysfunction,and septic shock).moreover,early implementation is important to increase treatment *** on a limited level of evidence,all current guidelines recommend using oseltamivir in this *** possibility of drug resistance should also be *** options include other antiviral drugs and combination therapies with monoclonal ***,it is not recommended to use corticosteroids in the initial treatment of these ***,the implementation of supportive measures for respiratory failure is ***

关键词： Influenza Pandemic Respiratory failure Acute respiratory distress syndrome Intensive care unit admission

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Diagnostic performance of serum interleukin-6 in pediatric acute appendicitis:a systematic review

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World Journal of Pediatrics 2022年第2期18卷 91-99页

作者： Javier Arredondo montero carlos Bardaji Pascual monica Bronte Anaut natalia Lopez-Andres Giuseppa Antona nerea martin-Calvo Pediatric Surgery Department Complejo Hospitalario de NavarraCalle Irunlarrea 331008 PamplonaNavarraSpain Pathology Department Complejo Hospitalario de NavarraPamplonaSpain Cardiovascular Translational Research Navarrabiomed(Miguel Servet Foundation)Complejo Hospitalario de Navarra(CHN)Universidad Publica de Navarra(UPNA)IdiSNAPamplonaSpain Department of Preventive medicine and Public Health Instituto de Investigacion Sanitaria de Navarra(IdiSNA).University of Navarra.PamplonaSpain CIBER Fisiopatologia de la obesidad y nutricion(CIBERobn) Health Institute CarlosⅢMadridSpain

Background Despite the radiological and analytical advances of the last decades,there is still a significant rate of diagnostic error in pediatric acute *** recent years,multiple biomarkers have emerged as potential diagnostic *** study aimed to examine the diagnostic performance of serum interleukin-6 in pediatric acute *** We conducted a systematic review of the literature that involved an extensive search in the main databases of medical bibliography(medline,Pubmed,Web of Science and SciELo).Two independent reviewers selected the relevant articles based on the previously defined inclusion and exclusion *** quality of the selected article was rated using the QUADAS2 *** extraction was performed by two independent *** The research in the medical bibliography databases resulted in 68 *** removed 26 *** the remaining 42 articles,we excluded 33 following the inclusion and exclusion *** the final 9 studies included in this review,8 provided measured serum interleukin-6 values,and all of them reported significant differences between groups,but inconsistent results regarding sensitivity and *** The diagnostic performance of interleukine-6 alone for the diagnosis of acute appendicitis in children is *** sensitivity and specificity of interleukine-6 for the diagnosis of non-complicated acute appendicitis in the pediatric population are moderate but increased in complicated *** seems to be a direct relationship between serum level of interleukin-6 and the hours of evolution of abdominal pain in children with acute *** increasingly widespread use of non-operative management of acute appendicitis warrants further exploration of the classificatory potential of this marker between complicated and uncomplicated *** consider that this may be an avenue of research to explore in the future.

关键词： Acute appendicitis Children Interleukin-6 Pediatric Sensitivity Specificity Systematic review

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A nETWoRK AnALYSIS oF THE 2010 FIFA WoRLD CUP CHAmPIon TEAm PLAY

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Journal of Systems Science & Complexity 2013年第1期26卷 21-42页

作者： CoTTA carlos moRA Antonio m mERELo Juan Julin mERELo-moLInA Cecilia Department of Computer Science University of Mlaga Department of Architecture and Computer Technology University of Granada

This paper analyzes the network of passes among the players of the Spanish team during the last FIFA World Cup 2010,where they emerged as the champion,with the objective of explaining the results obtained from the behavior at the complex network *** team is considered a network with players as nodes and passes as(directed) edges.A temporal analysis of the resulting passes network is also done,looking at the number of passes,length of the chain of passes,and to network measures such as player centrality and clustering *** of the last three matches(the decisive ones) indicate that the clustering coefficient of the pass network remains high,indicating the elaborate style of the Spanish *** effectiveness of the opposing team in negating the Spanish game is reflected in the change of several network measures over time,most importantly in drops of the clustering coefficient and passing length/speed,as well as in their being able in removing the most talented players from the central positions of the ***'s ability to restore their combinative game and move the focus of the game to offensive positions and talented players is shown to tilt the balance in favor of the Spanish team.

关键词：网络分析世界杯播放军队聚类系数移动游戏西班牙网络行为

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on the non-evolution of the dependence of black hole masses on bolometric luminosities for QSos

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Research in Astronomy and Astrophysics 2012年第3期12卷 249-259页

作者： martín López-Corredoira carlos m. Gutirrez Instituto de Astrofísica de Canarias Departamento de Astrofísica Universidad de La Laguna

There are extremely luminous quasi stellar objects （QSos） at high redshift which are absent at low redshift. The lower luminosities at low redshifts can be understood as the external manifestation of either a lower Eddington ratio or a lower mass. To distinguish between both effects, we determine the possible dependence of masses and Eddington ratios of QSos with a fixed luminosity as a function of redshifts; this avoids the malmquist bias or any other selection effect. For the masses and Eddington ratios derived for a sample of QSos in the Sloan Digital Sky Survey, we model their evolution by a double linear fit separating the dependence on redshifts and luminosities. The validity of the fits and possible systematic effects were tested by the use of different estimators of masses or bolometric luminosities, and possible intergalactic extinction effects. The results do not show any significant evolution of black hole masses or Eddington ratios for equal luminosity QSos. The black hole mass only depends on the bolometric luminosity without significant dependence on the redshift as （m BH/10 9 m ）≈ 3.4 （L bol/10 47 erg s-1）0.65 on average for z ≤ 5. This must not be confused with the possible evolution in the formation of black holes in QSos. The variations of the environment might influence the formation of the black holes but not their subsequent accretion. It also leaves a question to be solved： Why are there not QSos with very high mass at low redshift？ A brief discussion of the possible reasons for this is tentatively pointed out.

关键词： accretion—methods: statistical—quasars: general

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mechanical behavior of iron ore tailings under standard compression and extension triaxial stress paths

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Journal of Rock mechanics and Geotechnical Engineering 2023年第7期15卷 1883-1894页

作者： Alexia Cindy Wagner João Paulo de Sousa Silva João Vítor de Azambuja Carvalho Ana Luisa Cezar Rissoli Pedro Pazzoto Cacciari Helder mansur Chaves Hugo carlos Scheuermann Filho nilo Cesar Consoli Universidade Federal do Rio Grande do Sul Porto Alegre90035-190Brazil mineral Development Centre Vale S.A.Santa Luzia33040-900Brazil Department of Civil Geological and Mining EngineeringPolytechnique MontrealMontrealH3C 3A7Canada

The disposal of mining tailings has increasingly focused on the use of dry *** structures offer more security since they use filtered and compacted *** of the construction method and the heights achieved,the material that compounds the structure can be subjected to different stress paths along the failure *** theoretical framework considered in the design of these structures generally is the critical state soil mechanics(CSSm).However,the data in the literature concerning the uniqueness of critical state line(CSL)is still controversial as the soil is subjected to different stress *** respect to tailings,this question is even more *** paper studies two tailings with different gradings due to the beneficial processes over extension and compression paths.A series of drained and undrained triaxial tests was conducted over a range of initial densities and stress *** the q-p'plane,different critical stress ratio(m)values were obtained for compression and extension stress ***,the critical state friction angle is very similar with a slightly higher critical state friction angle for extension *** stress path dependent CSLs were obtained in the n-lnp0 plane with the extension tests below the CSL defined in *** the fines content,the studied tailings presented very similar m and critical state friction angle ***,the fines content af-fects the volumetric behavior of the studied tailings and the CSLs on the n-lnp0 plane shift downwards with the increasing fines content for compression and extension *** relation to dilatancy analysis,the fines content did not present an evident influence on the dilatancy of the ***,different values of mean stress ratio n were obtained between compression and extension tests and can corroborate the existence of non-unique CSLs for these materials.

关键词： Iron ore tailings(IoTs) Dry stacking Critical state soil mechanics Extension tests Stress path

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Food allergy among mexican infants and preschoolers:prevalence and associated factors

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World Journal of Pediatrics 2023年第4期19卷 401-405页

作者： martín Bedolla-Barajas Jaime morales-Romero Rafael Sánchez-magallón Jorge Alejandro Valdez-Soto Tonatiuh Ramses Bedolla-Pulido carlos meza-López Allergy and Immunology Department Nuevo Hospital Civil de Guadalajara Dr.Juan I.MenchacaSalvador Quevedo y Zubieta No.740Colonia La Perla44340GuadalajaraJaliscoMéxico Institute of Public Health Universidad VeracruzanaXalapaMexico Pediatrics Department Nuevo Hospital Civil de Guadalajara Dr.Juan I.MenchacaGuadalajaraMexico

The number of people with food allergy(FA)has increased in recent decades,as has the number of foods to which they are *** of FA prevalence vary widely,likely because of different definitions,evaluated foods and geo-graphic *** developed countries,overall preva-lence estimates range from 1.9%to 7.2%[1,2].In Latin America,the prevalence of FA in children is between 1.4%and 5.3%[3-6],with important age-based variations(from 1.9%in infants to 0.4%in preschoolers)[7].FA is a complex trait in which the interplay of genetic and environmental fac-tors results in disease ***-known factors that increase atopic susceptibility include sex[8,9],age[10],and socioeconomic status[11-13].Furthermore,the asso-ciation between family history of allergies and childhood FA has been consistently reported in recent ***,a maternal history of allergic disease appears to be more strongly associated with FA than paternal history of atopy[14].

关键词： prevalence allergy allergic

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near shore seismic movements induced by seaquakes using the boundary element method

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Earthquake Engineering and Engineering Vibration 2017年第3期16卷 571-585页

作者： Alejandro Rodríguez-Castellanos manuel Carbajal-Romero norberto Flores-guzmán J.Efraín Rodríguez-Sánchez Andriy Kryvko instituto mexicano del petróleo eje central lázaro cárdenas 152Gustavo A.MaderoDistrito FederalMéxico escuela superior de ingeniería mecánicay eléctrica instituto politécnico nacionalupalmEdif.5Av.Instituto Politécnico Nacional S/NCol.LindavistaGustavo A.MaderoDistrito FederalMéxico escuela superior de ingeniería mecánicay eléctrica instituto politécnico nacionalunidad profesional azcapotzalcoDistrito FederalMéxico centro de investigación en matemáticas jalisco s/nmineral de valencianaguanajuatoMéxico

This study quantifies seismic amplifications in near-shore arising from seaquakes. Within the Boundary Element method, boundary elements are used to irradiate waves and force densities obtained for each element. Huygens′ Principle is implemented since the diffracted waves are constructed at the boundary from which they are radiated, which is equivalent to Somigliana′s theorem. Application of boundary conditions leads to a system of integral equations of the Fredholm type of second kind and zero order. Several numerical configurations are analyzed： The first is used to verify the present formulation with ideal sea floor configurations to estimate seismic amplifications. With the formulation verified, simple slope configurations are studied to estimate spectra of seismic motions. It is found that P-waves can produce seismic amplifications from 1.2 to 3.9 times the amplitude of the incident wave. SV-waves can generate seismic amplifications up to 4.5 times the incident wave. Another relevant finding is that the highest amplifications are at the shore compared to the ones at the sea floor.

关键词： seismic amplifications seaquake earthquake elastic waves boundary element method near shore cities

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