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Temperature changes in the root ecosystem affect plant functionality

Plant Communications 2023年第3期4卷 197-214页

作者： Mary Paz González-García carlos M.Conesa Alberto Lozano-Enguita Victoria Baca-González Bárbara Simancas Sara navarro-Neila María Sánchez-Bermúdez Isai Salas-González Elena Caro Gabriel Castrillo Juan C.del Pozo Centro de Biotecnología y Genómica de Plantas(UPM-INIA/CSIC) Universidad Politécnica de Madrid(UPM)-Instituto Nacional de Investigación y Tecnología Agrariay Alimentaria-CSIC(INIA/CSIC)Campus Montegancedo28223 Pozuelo de Alarcón(Madrid)Spain Departamento de Biotecnología-Biología Vegetal Escuela Técnica Superior de Ingeniería AgronómicaAlimentaria y de BiosistemasUniversidad Politécnica de Madrid(UPM)28040 MadridSpain Undergraduate Program in Genomic Sciences Center for Genomics SciencesUniversidad Nacional Autonóma de MéxicoAv.Universidad s/n.Col.ChamilpaCuernavaca 62210MorelosMéxico Future Food Beacon of Excellence&School of Biosciences University of NottinghamSutton BoningtonUK

Climate change is increasing the frequency of extreme heat events that aggravate its negative impact on plant development and agricultural *** experiments designed to study plant adaption to heat stress apply homogeneous high temperatures to both shoot and ***,this treatment does not mimic the conditions in natural fields,where roots grow in a dark environment with a descending temperature *** high temperatures severely decrease cell division in the root meristem,compromising root growth,while increasing the division of quiescent center cells,likely in an attempt to maintain the stem cell niche under such harsh ***,we engineered the TGRooZ,a device that generates a temperature gradient for in vitro or greenhouse growth *** root systems of plants exposed to high shoot temperatures but cultivated in theTGRooZ grow efficiently and maintain their functionality to sustain proper shoot growth and ***,gene expression and rhizosphere or root microbiomecomposition are significantly less affected in TGRooZ-grown roots than in high-temperature-grown roots,correlating with higher root *** data indicate that use of the TGRooZ in heat-stress studies can improve our knowledge of plant response to high temperatures,demonstrating its applicability from laboratory studies to the field.

关键词： heat stress root temperature gradient nutrition gene expression microbiome

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Increased incidence of and microbiologic changes in pyogenic liver abscesses in the Mexican population

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World Journal of Hepatology 2020年第10期12卷 816-828页

作者： Juanita Pérez-Escobar Wagner Ramirez-Quesada Daniel Alejandro Calle-Rodas Luis Alberto Chi-Cervera Nalu navarro-Alvarez Jorge Aquino-Matus Juan Pablo Ramírez-Hinojosa carlos Moctezuma-Velázquez Aldo Torre Department of Gastroenterology Instituto Nacional de Ciencias Médicas y Nutrición Salvador ZubiránMexico City 14080Mexico Department of Internal Medicine Hospital General Dr.Manuel Gea GonzálezMexico City 14080Mexico

BACKGROUND Pyogenic liver abscess(PLA)is a rare disease with an estimated incidence that varies widely across the globe,being as high as 115.4/100000 habitants in Taiwan and as low as 1.1-1.2/100000 habitants in Europe and *** though there are multiple microorganisms capable of producing an abscess in the liver,including Entamoeba histolytica,fungi,and viruses,most abscesses are derived from bacterial *** epidemiology of PLA in Mexico is currently *** To describe the clinical,demographic and microbiologic characteristics of PLA in *** This is a retrospective study carried out in two centers,and included patients seen between 2006 and 2018 with the diagnosis of pyogenic *** collected demographic,clinical,and microbiological information,treatment,complications,and outcomes.A logistic regression analysis was used to determine the association between different variables and mortality *** A total of 345 patients were included in this study.233(67.5%)had confirmed PLA,133(30%)patients had no positive culture and negative serology and 9(2.5%)had mixed *** mean age was 50 years(ranging from 16-97 years)and 63%were female.65%of the patients had positive cultures for Extended Spectrum Beta-Lactamases(ESBL)-Escherichia coli and Klebsiella *** was administered in 60%of *** most common sources of infection were ascending cholangitis and cholecystitis in 34(10%)and 31(9%),*** median length of hospital stay was 14 d.165 patients underwent percutaneous catheter *** inpatient mortality rate was 63%.Immunocompromised state[OR 3.9,95%CI:1.42-10.46],ESBL-Escherichia coli[OR 6.7,95%CI:2.7-16.2]and Klebsiella pneumoniae[OR 4-8,95%CI:1.6-14.4]predicted inpatient mortality by multivariate *** The prevalence of PLA is increasing in Mexico and has a very high mortality ***-Escherichia coli and Klebsiella pneumoniae are the most common microorganisms causing PLA

关键词： Liver abscess Pyogenic Mexican population Epidemiology Complications Outcomes Mortality

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Enhancement of Corrosion Resistance of NiCrFeBSi Coatings Obtained by Flame Thermal Spray Process Adding an Electroless Nickel Coating Ni-P

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Journal of Surface Engineered Materials and Advanced Technology 2017年第4期7卷 86-97页

作者： Arturo Barba-Pingarrón Raúl Valdez-navarro Félix Sánchez-De Jesus Ana María Bolarín-Miró Rafael González-Parra Alba Covelo-Villar Miguel ángel Hernández-Gallegos carlos Domínguez-Ríos Centro de Ingeniería de Superficies y Acabados (CENISA) Facultad de Ingeniería Universidad Nacional Autónoma de México 2 Nivel Edificio “T” (Bernardo Quintana) Circuito Exterior Ciudad Universitaria Ciudad de México México área Académica de Ciencias de la Tierra y Materiales Universidad Autónoma del Estado de Hidalgo Pachuca México Centro de Investigación en Materiales Avanzados Chihuahua México

Flame Thermal Spray (FTS) coatings frequently show some porosity and reduced adherence to substrate, which affect its properties, especially its corrosion resistance. In this work, the corrosion resistance of AISI 1018 carbon steel coated by different methods is compared: electroless nickel (EN) coating, NiCrFeBSi obtained by FTS, duplex coatings of an EN deposit on a layer of NiCrFeBSi obtained by FTS and a layer of NiCrFeBSi on an EN deposit. The coatings were characterized using optical microscopy and scanning electron microscopy techniques, EDS microprobe microanalysis, roughness as well as electrochemical polarization tests to obtain the corrosion rate. The results show the enhancement in the corrosion resistance in saline medium of the duplex coatings, especially of the EN coating on FTS layer.

关键词： Flame Thermal Spray Coatings Electroless Nickel Plating Duplex Coatings Corrosion Resistance

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Advances in cellular technology in the hematology field: What have we learned so far?

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World Journal of Stem Cells 2015年第1期7卷 106-115页

作者： Gustavo Torres de Souza Claudineia Pereira Maranduba Camila Maurmann de Souza Danielle Luciana Aurora Soares do Amaral Francisco carlos da Guia Rafaella de Souza Salomao Zanette Joao Vitor Paes Rettore Natana Chaves Rabelo Lucas Mendes Nascimento Icaro Franca navarro Pinto Julia Boechat Farani Abrahao Elias Hallack Neto fernando de Sa Silva carlos Magno da Costa Maranduba Angelo Atalla Laboratory of Human Genetics and Cell Therapy Biology Department Biological Sciences Institute Federal University of Juiz de Fora Juiz de Fora 36036-900Brazil Haematology and Bone Marrow Transplantation Service Medical Clinic Department University Hospital Federal University of Juiz de Fora Juiz de Fora 36036-900 Brazil

Despite the advances in the hematology field, blood transfusion-related iatrogenesis is still a major issue to be considered during such procedures due to blood antigenic incompatibility. This places pluripotent stem cells as a possible ally in the production of more suitable blood products. The present review article aims to provide a comprehensive summary of the state-of-theart concerning the differentiation of both embryonic stem cells and induced pluripotent stem cells to hematopoietic cell lines. Here, we review the most recently published protocols to achieve the production of blood cells for future application in hemotherapy, cancer therapy and basic research.

关键词： Hematopoietic stem cells Progenitor Induced pluripotent stem cells Reprogramming Embryonic stem cells

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Different profile of cytokine synthesis according to the severity of acute pancreatitis

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World Journal of Gastroenterology 2005年第34期11卷 5309-5313页

作者： Raquel Laveda Juan Martínez carlos Mu■oz Juan carlos Penalva Jesus Sáez Germán Belda Salvador navarro Faust Feu Anton Mas José Ma Palazón José Sánchez-Payá José Such Miguel Pérez-Mateo Gastroenterology Department and Liver Unit Hospital General Universitario Alicante Spain Institut de Malalties Digestives Hospital Clínic. Institut d'Investigacions Biomédiques August Pi I Sunyer Barcelona Spain

AIM: To evaluate the cellular synthetic ability of cytokines involved in pro- and anti-inflammatory reactions in patients with ***: Sixty-seven patients with AP (16 severe,51 mild) and 10 controls were included in the *** of whole blood were performed in samples obtained within the first 72 h from the onset of *** levels of interleukins (IL) 6, 8, 10, and TNF-α were measured at baseline and in the supernatant of cultures with (functional reserve) or without stimulation with ***: Basal levels of cytokines were significantly higher in patients with severe AP. A significant increase of all pro-inflammatory cytokines vs basal levels was observed in the supernatant after 24 h of whole blood cultures in patients, but not in controls. In contrast, IL-10 increased significantly in the supernatant of cultures only in patients with mild AP. Cells showed a statistically significant functional reserve for all IL in patients with mild, but only for pro-inflammatory cytokines in patients with severe ***: A marked activation of immune system may be observed in patients with AP, being balanced between pro- and anti-inflammatory cytokines in patients with mild but not severe AP. A reduced functional reserve for the synthesis of IL-10 may be observed in patients with severe AP, which might lead to a worst prognosis.

关键词： Acute pancreatitis Cytokines Severity Wholeb lood

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Distributed Differences Structures Underlie Gating between the Kin Channel KAT1 and the Kout Channel SKOR

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Molecular Plant 2010年第1期3卷 236-245页

作者： Janin Riedelsberger Tripti Sharma Wendy Gonzalez Pawel Gajdanowicz Samuel Elias Morales-navarro carlos Garcia-Mata Bernd Mueller-Roeber fernando Danilo Gonzalez-Nilo Michael R. Blatt Ingo Dreyer Universitat Potsdam Institut for Biochemie und Biologie Molekularbiologie Heisenberg-Gruppe Biophysik und Molekulare Pflanzenbiologie BPMPB Karl-Liebknecht-Strasse 24-25 Haus 20 D-14476 Potsdam-Golm Germany Max-Planck Institute of Molecular Plant Physiology 14476 Potsdam-Golm Germany Centro de Bioinformatica y Simulacion Molecular Universidad de Talca Casilla 721 Talca Chile Laboratory of Plant Physiology and Biophysics IBLS Plant Sciences Bower Building University of Glasgow Glasgow G12 8QQ UK Laboratorio de Fisiologia Molecular e Integrativa Institutos de Investigaciones Biologicas Universidad Nacional de Mar del Plata 7600 Mar del Plata Buenos Aires Argentina Universitat Potsdam Institut for Biochemie und Biologie Abteilung Molekularbiologie 14476 Potsdam-Golm Germany

The family of voltage-gated （Shaker-like） potassium channels in plants includes both inward-rectifying （Kin） channels that allow plant cells to accumulate K＋ and outward-rectifying （Kout） channels that mediate K＋ efflux. Despite their dose structural similarities, Kin and Kout channels differ in their gating sensitivity towards voltage and the extracellular K＋ concentration. We have carried out a systematic program of domain swapping between the Kout channel SKOR and the Kin channel KAT1 to examine the impacts on gating of the pore regions, the S4, S5, and the S6 helices. We found that, in particular, the N-terminal part of the S5 played a critical role in KAT1 and SKOR gating. Our findings were supported by molecular dynamics of KAT1 and SKOR homology models. In silico analysis revealed that during channel opening and closing, displacement of certain residues, especially in the S5 and S6 segments, is more pronounced in KAT1 than in SKOR. From our analysis of the S4-S6 region, we conclude that gating （and K＋-sensing in SKOR） depend on a number of structural elements that are dispersed over this -145-residue sequence and that these place additional constraints on configurational rearrangement of the channels during gating.

关键词： Arabidopsis K+ channel outward rectifier inward rectifier channel protein structure channel protein-cation interaction gating, K+-dependent.

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Analysis of tumor-draining vein secretome: A direct access to tumor-derived extracellular vesicles in surgical lung cancer patients

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BIOCELL 2023年第5期47卷 951-957页

作者： YANGYI HE DAVID SANCHEZ-LORENTE MELISSA ACOSTA-PLASENCIA MARC BOADA ANGELA GUIRAO RAMON MMARRADES LAUREANO MOLINS ALFONS navarro Molecular Oncology and Embryology Laboratory Department of Surgery and Surgical SpecializationsHuman Anatomy UnitFaculty of Medicine and Health SciencesUniversitat de Barcelona(UB)Barcelona08036Spain School of Basic Medical Sciences Chengdu UniversityChengdu610106China Department of Thoracic Surgery Hospital Clínic de BarcelonaUniversitat de Barcelona(UB)Barcelona08036Spain Thoracic Oncology Unit Hospital ClinicBarcelona08036Spain Institut d’Investigacions Biomèdiques August Pi i Sunyer(IDIBAPS) Barcelona08036Spain Department of Pneumology Institut Clínic Respiratori(ICR)Hospital Clínic de BarcelonaUniversitat de Barcelona(UB)Barcelona08036Spain Centro de Investigación Biomédica en Red de Enfermedades Respiratorias(CIBERES) Instituto de Salud Carlos IIIMadrid28029Spain

Tumor-secreted extracellular vesicles(EVs)participate in the metastasis process through different mechanisms,including the preparation of the pre-metastatic niche to grant circulating tumor cells(CTCs)implantation and *** study of the metastasis process through the analysis of CTCs and tumor-derived EVs is difficult because of the dilution grade of these elements in peripheral *** early-stage lung cancer patients,the tumor-secreted products are even more *** attractive strategy in surgical lung cancer patients is to purify them from a pulmonary tumor-draining vein where they are *** information obtained from the analysis of EVs and CTCs purified from this source could give more accurate information about tumor biology and could be an important source of biomarkers to identify patients at high risk of relapse after curative surgery.

关键词： Lung cancer Liquid biopsy Tumor-draining vein Extracellular vesicles Exosomes

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HLA-DQ:Celiac disease vs inflammatory bowel disease

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World Journal of Gastroenterology 2018年第1期24卷 96-103页

作者： Marta Maia Bosca-Watts Miguel Minguez Dolores Planelles Samuel navarro Alejandro Rodriguez Jesus Santiago Joan Tosca Francisco Mora Disease Department of the University Clinic Hospital of Valencia University of ValenciaValencia 46017Spain Histocompatibility Department of the Transfusion Center of the Valencian Community Valencia 46014Spain Pathology Department of the University Clinic Hospital of Valencia University of ValenciaValencia 46017Spain Digestive Disease Department of the Hospital Virgen del Castillo of Yecla Yecla 30510Spain Digestive Disease Department of the Hospital de Manises Valencia 46940Spain Digestive Disease Department of the University Clinic Hospital of Valencia University of ValenciaValencia 46017Spain

AIM To determine the genetic predisposition to celiacdisease(Ce D) in inflammatory bowel disease(IBD) patients by quantifying the frequency of Ce D-related human leucocyte antigen(HLA)(HLA-Ce D: HLA-DQ2 and-DQ8) in IBD patients globally, by type of IBD and gender, and by calculating the protective/risk contribution of these haplotypes in the development of the IBD *** We conducted a prospective study with IBD patients from our Unit. Clinical information was gathered and blood was tested for HLA-CeD. The control group was made up of unrelated Valencian organ ***1034 subjects were analyzed: 457 IBD [207 ulcerative coliti(UC) and 250 Crohn's disease(CD)] patients and 577 healthy controls. 39% of the controls and 34% of the patients had HLA-Ce D(P = 0.0852). HLA-DQ2 was less frequent in UC patients(P = 0.0287), and HLA-DQ8 in CD(P = 0.0217). In women with UC, the frequency of DQ2.5 cis(DQB1*02:01-DQA1*05:01) was reduced ≥ 50% [P = 0.0344; preventive fraction(PF) = 13%]. PFs(7%-14%) were obtained with all HLACe D haplotypes. HLA DQB1*02:02-DQA1*02:01(HLADQ2.2) was more frequent in CD patients with respect to controls(P = 0.001) and UC patients(etiological fraction = 15%).CONCLUSION HLA-CeD is not more frequent in IBD patients, with an even lower frequency of HLA-DQ2 and-DQ8 in UC and CD respectively. HLA-DQ2.5 confers protection from the development of UC, especially in women, and HLADQ8 does so for the appearance of CD. HLA-DQ2.2 is present in 34% of the CD patients and may constitute a genetic risk factor for CD development.

关键词： Genetic predisposition Celiac disease Inflammatory bowel disease Crohn's disease Human leucocyte antigen Ulcerative colitis

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Application of a Sulfur Removal Hydrometallurgical Process in a Lead-Acid Battery Recycling Plant in Costa Rica

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Open Journal of Air Pollution 2017年第1期6卷 18-26页

作者： Marta navarro-Monge Germain Esquivel-Hernandez Jose Pablo Sibaja Brenes Jose carlos Mora-Barrantes Ricardo Sanchez-Murillo Juan Valdes-Gonzalez Pablo Bolanos-Ulloa Atmospheric Laboratory Chemistry DepartmentNational University of Costa RicaHerediaCosta Rica Chemistry Department National University of Costa RicaHerediaCosta Rica Pb Metals S.A. CartagoCosta Rica

This study presents the implementation of a desulphurization process for lead recycling under different chemical and physical conditions using pyro-metallurgical processes. Desulphurization was done using a hydrometallurgical process using sodium carbonate as a desulphurization agent and different lead-bearing loads compositions. Waste characterization included: SO2 concentrations in the stack emissions, total lead content in the furnace ash, the total lead content in the slag, and the toxicity characteristic leaching procedure (TCLP). A significant reduction in SO2 emissions was achieved (~55% reduction) where mean SO2 concentrations changed from 2193 ± 135 ppm to 1006 ± 62 ppm after the implementation of the modified processes. The desulfurized lead paste (i.e. the metallic fraction lead of the battery) of the modified process exhibited an improvement in the concentration of the lead in the TCLP test, with an average value of 1.5 ppm which is below US EPA limit of 5 ppm. The traditional process TCLP mean value for the TCLP was 54.2 ppm. The total lead content in the bag house ashes shows not significant variations, when comparing the desulphurization (67.6% m/m) and non-desulphurization process (64.9% m/m). The total lead mean content in the slag was higher in the desulphurization process (2.49% m/m) than the traditional process (1.91% m/m). Overall, the implementation of a new desulphurization method would potentially increase the operation costs in 10.3%. At the light of these results, a combination of hydrometallurgical and pyro-metallurgical processes in the recycling of lead-acid batteries can be used to reduce the environmental impact of these industries but would increase the operational costs of small lead recyclers.

关键词： Lead-Acid Battery Recycling Hydrometallurgical and Pyro-Metallurgical Processes Hazardous Waste Management

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Thoracoscopic esophagectomy is related to better outcomes in early adenocarcinoma of esophagogastric junction tumors

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World Journal of Gastrointestinal Endoscopy 2021年第8期13卷 319-328页

作者： Flavio Roberto Takeda carlos de Almeida Obregon Yasmin Peres navarro Diogo Turiani Hourneaux Moura Ulysses Ribeiro Jr Rubens Antonio Aissar Sallum Ivan Cecconello Department of Gastroenterology University of São Paulo Medical SchoolSão Paulo 05403-000Brazil

BACKGROUND Thoracoscopic esophagectomy is related to an extended lymphadenectomy,and a high number of retrieved lymph nodes,compared to the transhiatal approach;however,its association with an improvement in overall survival(OS)is *** To compare thoracoscopic esophagectomy with transhiatal esophagectomy in patients with adenocarcinoma of the esophagogastric junction(AEGJ)in terms of survival,number of lymph nodes,and *** In total,147 patients with AEGJ were selected retrospectively from 2002 to 2019,and divided into Group A for thoracoscopic esophagectomy,and group B for transhiatal ***,disease-free survival,postoperative complications,and number of nodes,were similarly *** One hundred and thirty(88%)were male;the mean age was 64 *** A had a mean age of 61.1 years and group B 65.7 years(P=0.009).Concerning the extent of lymphadenectomy,group A showed a higher number of retrieved lymph nodes(mean of 31.89±8.2 vs 20.73±7;P<0.001),with more perioperative complications,such as hoarseness,surgical site infections,and respiratory *** both groups had similar OS rates,subgroup analysis showed better survival of transthoracic esophagectomy in patients with earlier *** Both methods are safe,having similar morbidity and mortality *** thoracoscopic esophagectomy allows a more extensive resection of the lymph nodes and may have better oncological outcomes during earlier stages of the *** studies are warranted to better evaluate these findings.

关键词： Adenocarcinoma Esophagogastric junction Transhiatal Thoracoscopic Lymph nodes Surgery

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