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Health 2013年第1期5卷 119-124页

作者： Rosileia Marinho Quadros Paulo Henrique Exterckotter Weiss Geison Willian Ezequiel Renato Batista Tamanho Geanice Lepo Marcio Rodrigues da silva carlos roberto joao da silva junior Flavio Antonio Pacheco de Araujo Luiz Claudio Miletti Universidade do Estado de Santa Catarina LagesBrazil

The knowledge of the epidemiology of parasitic infections in stray and domestic animals, especially of its incidence and prevalence, is fundamental to adopting effective prophylactic measures. Stray dogs play an important role in environmental contamination favoring the transmission cycle of zoonotic agents. Among the parasitic infections that affect humans, Giardia duodenalis is the most common intestinal protozoa and was designated as a re-emerging infectious disease. This study aimed to determine the prevalence of G. duodenalis in dogs siezed by the Center for Control of Zoonoses (CCZ) of the city of Lages, Santa Catarina, Brazil using two diagnostic techniques. In 357 stool samples analysed, the prevalence of G. duodenalis cysts was 5.3% (19/357) and 4.8% (17/357) detected by floatation and sedimentation techniques, respecttively. No correspondence between gender and age was found among the methods used for analyzing the infected dogs in this study. Our data suggested that two diagnostic techniques should be used in a complementary way to ensure that false negatives are not neglected.

关键词： Giardia Duodenalis Dogs Zoonoses Brazil

Antiglycation and antioxidant activities of the crude extract and saponin fraction of Tribulus terrestris before and after microcapsule release

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Journal of Integrative Medicine 2022年第2期20卷 153-162页

作者： Célia Cristina Malaguti Figueiredo Amanda da Costa Gomes Filipe Oliveira Granero joao Luiz Bronzel junior Luciana Pereira silva Valdecir Farias Ximenes Regildo Márcio Goncalves da silva Institute of Chemistry São Paulo State UniversityAraraquaraSão Paulo 14800-060Brazil Educational Foundation of the City of Assis AssisSão Paulo 19807-130Brazil Department of Chemistry School of SciencesSão Paulo State UniversityBauruSão Paulo 17033-360Brazil Department of Biotechnology School of SciencesHumanities and LanguagesSão Paulo State UniversityAssisSão Paulo 19806-900Brazil

Objective:The present study investigated antiglycation and antioxidant activities of crude dry extract and saponin fraction of Tribulus *** also developed a method of microencapsulation and evaluated antiglycation and antioxidant activities of crude dry extract and saponin fraction before and after microcapsule ***:Antiglycation activity was determined by relative electrophoretic mobility(REM),free amino groups and inhibition of advanced glycation end-product(AGE)*** activity was determined by 2,2-diphenyl-1-picrylhydrazyl(DPPH),ferric ion-reducing antioxidant power(FRAP),nitric oxide(NO)and thiobarbituric acid reactive species(TBARS)*** were prepared using maltodextrin as wall material and freeze-drying as encapsulation *** characterization of microcapsules was evaluated by scanning electron microscopy,and encapsulation efficiency and microcapsule release were determined by total saponins *** and antioxidant assays were performed using crude dry extract and saponin fraction of *** before and after ***:Saponin fraction showed an increase of 32.8%total ***-performance liquid chromatography-mass spectrometry analysis showed the presence of saponins in the obtained *** evaluation by REM demonstrated that samples before and after release presented antiglycation activity similar to bovine serum albumin treated with ***,samples inhibited AGE formation,highlighting treatment with saponin fraction after release(89.89%).Antioxidant tests demonstrated antioxidant activity of the *** dry extract before encapsulation presented the highest activities in DPPH(92.00%)and TBARS(32.49%)*** fraction before encapsulation in FRAP test(499μmol Trolox equivalent per gram of dry sample)and NO test(15.13μmol nitrite formed per gram of extract)presented the highest ***:This study presen

关键词： Glycation Microencapsulation Oxidative stress Saponins Tribulus terrestris

Appropriateness of the study of iron deficiency anemia prior to referral for small bowel evaluation at a tertiary center

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World Journal of Gastroenterology 2017年第24期23卷 4444-4453页

作者： Jaime Pereira Rodrigues Rolando Pinho Joana silva Ana Ponte Mafalda Sousa joao carlos silva joao Carvalho Department of Gastroenterology Centro Hospitalar Vila Nova de Gaia/EspinhoRua Conceicao FernandesVila Nova de Gaia

To evaluate the adequacy of the study of iron deficiency anemia (Ida) in real life practice prior to referral to a gastroenterology department for small bowel evaluation. METHODSAll consecutive patients referred to a gastroenterology department for small bowel investigation due to iron deficiency anemia, between January 2013 and December 2015 were included. Both patients referred from general practitioners or directly from different hospital departments were selected. Relevant clinical information regarding prior anemia workup was retrospectively collected from medical records. An appropriate pre-referral study was considered the execution of esophagogastroduodenoscopy (EGD) with Helicobacter pylori (H. pylori) investigation, colonoscopy with quality standards (recent, total and with adequate preparation) and celiac disease (CD) screening (through serologic testing and/or histopathological investigation). RESULTSA total of 77 patients (58.4% female, mean age 67.1 ± 16.7 years) were included. Most (53.2%) patients were referred from general practitioners, 41.6% from other hospital specialties and 5.2% directly from the emergency department. The mean pre-referral hemoglobin concentration was 8.8 ± 2.0 g/dL and the majority of anemias had microcytic (71.4%) and hypochromic (72.7%) characteristics. 77.9% of patients presented with an incomplete pre-referral study: EGD in 97.4%, with H. pylori investigation in 58.3%, colonoscopy with quality criteria in 63.6%, and CD screening in 24.7%. Patients with an appropriate study at the time of referral were younger (48.7 ± 17.7 vs 72.3 ± 12.3 years, P da was small bowel angioectasia (18.2%) but additional causes were also found in the upper and lower ga

关键词： Iron deficiency anemia Colonoscopy Celiac disease Esophagogastroduodenoscopy Helicobacter pylori Small bowel

Biological and chemical attributes of soils under forest species in Northeast Brazil

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Journal of Forestry Research 2020年第5期31卷 1959-1973页

作者： Olmar Baller Weber Maria Catia Barroso da silva Cristiane Figueira da silva joao Alencar de Sousa carlos Alberto Kenji Taniguch Deborah dos Santos Garruti Ricardo Espindola Romero Embrapa Tropical Agroindustry Rua Dra.Sara Mesquita2270PiciFortalezaCeara60511-110Brazil Centro de Ciencias e Tecnologia Universidade Estadual do CearaAv.Dr.Silas Munguba1700Campus do ItaperiFortaleza 60740-000Brazil Universidade Federal Rural do Rio de Janeiro BR 465Km 7SeropedicaRio De Janeiro 23890-000Brazil Depto Ciencia do Solo Universidade Federal do CearaCampus do PiciFortaleza 60021-970Brazil

Timber forests contribute to the sustainable development of the biomes in tropical *** aim of this study was to evaluate the biological and chemical properties of the soil as a consequence of the cover with native and non-native species in the Acarau basin,a transition area from the coast to the Brazilian semi-arid *** planted with four native species(Anadenanthera colubrina,Astronium fraxinifolium,Handroanthus impetiginosus,Colubrina glandulosa)and three exotic species(Acacia mangium,Casuarina equisetifolia,Eucalyptus urophylla)plus a non-forested agricultural area were evaluated for organic carbon contents,and microbial and chemical soil *** levels of soil organic carbon were highest in *** and *** *** basal soil respiration was observed but the microbial biomass was particularly low in the non-forested *** the ***,***,and *** plantations,elevated soil metabolic quotients were *** *** and *** plantations had the highest levels of easily extracted-glomalin related soil *** species affect concentrations of essential nutrients and the biological quality of the soil in different *** can also improve the biological and chemical properties of the soil in the coastal plains of tropical regions.

关键词： Soil quality Brazilian timber species Eucalyptus Acacia Casuarina

Guiding strategies for breeding vegetable cultivars

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Agricultural Sciences 2014年第1期5卷 9-32页

作者： joao carlos da silva Dias Technical University of Lisbon Instituto Superior de Agronomia Tapada da Ajuda Lisbon Portugal

Vegetables are considered essential for well-balanced diets. The production and marketing of vegetables crops are undergoing continuous change globally. This is mainly due to the growing demands of consumers for safe and healthy vegetables, increased urbanisation of societies, and the growth in scale and influence of supermarkets chains. Horticultural science can respond to many of these challenges through research, breeding and innovation that can seek to gain more efficient methods of crop production, refined post-harvest storage and handling methods, newer and higher value vegetable cultivars and demonstration of their health benefits. Vegetable breeding has to address and satisfy the needs of both the consumer and the producer. Innovation in vegetable breeding depends on specific knowledge, the development and application of new technologies, access to genetic resources, and capital to utilise them. The driving force behind this innovation is acquiring or increasing market share. Access to technology, as well as biodiversity, is essential for the development of new vegetable cultivars. A few multinational corporations, whose vast economic power has effectively marginalized the role of public sector breeding as well as local, small/medium-scale seed companies, dominate the global vegetable seed trade. For most vegetable crops, only a few multinational seed corporations are controlling large part of the world market. This situation makes a growing part of the global vegetable supply dependent on a few seed providers. The multinational seed corporations ensued from merging some small or medium vegetable breeding programs to reduce costs. There may be fewer vegetable breeders in the future and growers will rely on seeds with a narrow genetic base. In order to meet future needs of vegetable breeders it is important that educational programs incorporate rapidly changing new technologies with classical content and methods. Active and positive connections between the pr

关键词： Vegetables Horticulture Health Benefits Marketing Trading Breeding Year-Round Production Sustainability

Nutritional and Health Benefits of Carrots and Their Seed Extracts

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Food and Nutrition Sciences 2014年第22期5卷 2147-2156页

作者： joao carlos da silva Dias Instituto Superior de Agronomia University of LisbonLisboaPortugal

Carrot is a root vegetable with carotenoids, flavonoids, polyacetylenes, vitamins, and minerals, all of which possess numerous nutritional and health benefits. Besides lending truth to the old adage that carrots are good for eyes, carotenoids, polyphenols and vitamins present in carrot act as antioxidants, anticarcinogens, and immunoenhancers. Anti-diabetic, cholesterol and cardiovascular disease lowering, anti-hypertensive, hepatoprotective, renoprotective, and wound healing benefits of carrot have also been reported. The cardio- and hepatoprotective, anti-bacterial, anti-fungal, anti-inflammatory, and analgesic effects of carrot seed extracts are also noteworthy. All are discussed in this review article.

关键词： daucus carota Chemical Composition Antioxidants Phytochemicals Disease Prevention

Lactobacillus bulgaricus inhibits colitis-associated cancer via a negative regulation of intestinal inflammation in azoxymethane/dextran sodium sulfate model

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World Journal of Gastroenterology 2020年第43期26卷 6782-6794页

作者： Denise Sayuri Calheiros Silveira Luciana Chain Veronez Luis carlos Lopes-junior Elen Anatriello Mariangela Ottoboni Brunaldi Gabriela Pereira-da-silva Department of Biochemistry and Immunology University of São Paulo at Ribeirão Preto Medical SchoolRibeirão Preto 14049-900SPBrazil Health Sciences Center Federal University of Espírito Santo-UFESVitória 29043-900ESBrazil Institute of Science and Technology Federal University of São PauloUNIFESPSão Josédos Campos 12231-280SPBrazil Department of Pathology and Forensic Medicine University of São PauloRibeirao Preto 14040-902SPBrazil Department of Maternal-Infant Nursing and Public Health University of São Paulo at Ribeirão Preto College of NursingRibeirão Preto 14040-902SPBrazil

BACKGROUND Colitis-associated cancer(CAC)accounts for 2%-3%of colorectal cancer(CRC)cases preceded by inflammatory bowel diseases(IBD)such as Crohn's disease and ulcerative *** microbiota has been reported to play a central role in the pathogenesis of IBD and ***,numerous prebiotics and probiotics have being investigated as antitumor agents due to their capacity to modulate inflammatory *** studies have indicated that lactic acid bacteria could be successfully used in managing sporadic CRC,however little is known about their role in *** To investigate the effect of the probiotic Lactobacillus bulgaricus(***)during the development of an experimental model of colitis associated colon cancer(CAC).METHODS C57BL/6 mice received an intraperitoneal injection of azoxymethane(10 mg/kg),followed by three cycles of sodium dextran sulphate diluted in water(5%w/v).Probiotic group received daily *** inflammation was determined by scoring clinical *** levels were determined from colon and/or tumor samples by ELISA BD OptEIATM *** level of significance was set at P<*** were generated and statistical analysis performed using the software GraphPad Prism *** *** treatment inhibited of total tumor volume and mean size of *** addition,the probiotic also attenuated the clinical signs of intestinal inflammation inducing a decrease in intestinal and tumor levels of IL-6,TNF-α,IL-17,IL-23 and IL-1β.CONCLUSION Our results suggest a potential chemopreventive effect of probiotic on *** regulates the inflammatory response and preventing CAC.

关键词： Lactobacillus bulgaricus Colitis-associated cancer Colorectal cancer Carcinogenesis Probiotics Inflammation

Plant Breeding for Harmony between Modern Agriculture Production and the Environment

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Agricultural Sciences 2015年第1期6卷 87-116页

作者： joao carlos da silva Dias Instituto Superior de Agronomia University of LisbonLisbonPortugal

The world population is estimated to be 9.2 billion in 2050. To sufficiently feed these people, the total food production will have to increase 60% - 70%. Climate models predict that warmer tem-peratures and increases in the frequency and duration of drought during the present century will have negative impact on agricultural productivity. These new global challenges require a more complex integrated agricultural and breeding agenda that focuses on livelihood improvement coupled with agro-ecosystem resilience, eco-efficiency and sustainability rather than just on crop productivity gains. Intensifying sustainability agro-ecosystems by producing more food with lower inputs, adapting agriculture to climate change, conserving agro-biodiversity through its use, and making markets to work for the small farmers are needed to address the main issues of our time. Plant breeding has played a vital role in the successful development of modern agriculture. Development of new cultivars will be required while reducing the impact of agriculture on the environment and maintaining sufficient production. Conventional plant breeding will remain the backbone of crop improvement strategies. Genetic engineering has the potential to address some of the most challenging biotic constraints faced by farmers, which are not easily addressed through conventional plant breeding alone. Protective measures and laws, especially patenting, must be moderated to eliminate coverage so broad that it stifles innovation. They must be made less restrictive to encourage research and free flow of materials and information. Small farmers have an important role in conserving and using crop biodiversity. Public sector breeding must remain vigorous, especially in areas where the private sector does not function. This will often require benevolent public/private partnerships as well as government support. Active and positive connections between the private and public breeding sectors and large-scale gene banks ar

关键词： Breeding Modern Agriculture Climate Changes Biodiversity Environment Ecosystems Transgenic Crops Small Farmers

The assessment of climatic,environmental,and socioeconomic aspects of the Brazilian Cerrado

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Ecological Processes 2023年第1期12卷 274-285页

作者： Washington Luiz Félix Correia Filho JoséFrancisco de Oliveira-Júnior Dimas de Barros Santiago Hazem Ghassan Abdo Hussein Almohamad Ahmed Abdullah Al Dughairi carlos Antonio da silva junior Postgraduate Program in Environmentrics(PPGAmb) Institute of MathematicsStatisticsand Physics(IMEF)Federal University of Rio Grande(FURG)Rio GrandeRio Grande do Sul 96203-900Brazil Institute of Atmospheric Sciences(ICAT) Federal University of Alagoas(UFAL)MaceióAlagoas 57072-260Brazil Postgraduate Program in Biosystems Engineering(PGEB) Federal Fluminense University(UFF)NiteróiRio de Janeiro 24220-900Brazil Postgraduate Program in Meteorology Unidade Acadêmica de Ciências Atmosféricas(UACA)Federal University of Campina Grande(UFCG)Campina GrandeParaíba 58429-140Brazil Geography Department Faculty of Arts and HumanitiesTartous UniversityTartousSyria Geography Department Faculty of Arts and HumanitiesDamascus UniversityDamascusSyria Geography Department Faculty of Arts and HumanitiesTishreen UniversityLat-takiaSyria Department of Geography College of Arabic Language and Social StudiesQassim UniversityBuraydah 51452Saudi Arabia Departamento de Geografia State University of Mato Grosso(UNEMAT)SinopMato Grosso 78555-000Brazil

Background The Cerrado is the most biodiverse savanna and maintains other *** of its significance,this paper evaluated the Brazilian Cerrado’s climatic,environmental,and socioeconomic aspects using remote sensing data and spatial statistics(correlation analysis and principal components analysis—PCA).Following the measures of sample adequacy(MSA)and Kaiser–Meyer–Olkin(KMO)tests,seventeen variables were *** The MSA revealed that the dataset had a good quality(0.76),and nine variables were selected:elevation,evapotranspiration,active fires,Human Development Index(HDI),land use and land cover(LULC;shrubland and cropland/rainfed),rainfall(spring and autumn),and *** correlation matrix indicated a positive(negative)association between HDI and autumn rainfall(HDI and active fires)with a value of 0.77(-0.55).The PCA results determined which three principal components(PC)were adequate for extracting spatial patterns,accounting for 68.02%of the total variance with respective values of 38.59%,16.89%,and 12.5%.Due to economic development and agribusiness,Cerrado’s northern(central,western,and southern)areas had negative(positive)score HDI values,as shown in ***(rainfall—spring and fall)and environmental(cropland/rainfed and shrubland)aspects dominated the PC2,with negative scores in northern and western portions due to the transition zone between Amazon and Cerrado biomes caused by rainfall *** the other hand,environmental aspects(LULC-shrubland and elevation)influenced the PC3;areas with high altitudes(>500 m)received a higher *** Agricultural expansion substantially affected LULC,leading to deforestation-caused suppression of native vegetation.

关键词： Environment Agribusiness Active fire Human Development Index Land use and land cover

19-year remotely sensed data in the forecast of spectral models of the environment

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International Journal of Digital Earth 2021年第8期14卷 1040-1066页

作者： Fernando Saragosa Rossi carlos Antonio da silva junior JoséFrancisco de Oliveira-Júnior Paulo Eduardo Teodoro Luciano Shozo Shiratsuchi Mendelson Lima Larissa Pereira Ribeiro Teodoro Auana Vicente Tiago Guilherme Fernando Capristo-silva State University of Mato Grosso(UNEMAT) Alta FlorestaMato GrossoBrazil Department of Geography State University of Mato Grosso(UNEMAT)SinopMato GrossoBrazil cFederal University of Alagoas(UFAL) MaceióAlagoasBrazil dFederal University of Mato Grosso do Sul(UFMS) Chapadão do SulMato Grosso do SulBrazil eAgCenter School of PlantEnvironmentaland Soil SciencesLouisiana State University(LSU)Baton RougeLouisianaUSA fPostgraduate Program in Agronomy Federal University of Mato Grosso(UFMT)SinopMato GrossoBrazil

The aims of this study were:i)to compare no-till areas in two municipalities located in different regions of Brazil,along with the influence on CO_(2)Flux and GPP,and ii)to verify the difference between environmental factors followed by the trends of these variables regarding future scenarios(ARIMA time-series model number).The study was carried out in two areas with different latitudes in the municipalities of Sinop-MT and Passo Fundo-RS,both in Brazil.A time series of 19 years was performed with data acquired by remote sensing from the following satellites:i)Landsat-8(OLI and TIRS),and ii)TERRA/AQUA(MODIS).The results propound that the spectro-temporal variables are directly influenced by soil management and agricultural practices over the observation time,with a satisfactory correlation in future predictions of the variables for the next ten years,in which presented that the variation of GPP and albedo values for the two study sites would gradually increase until 2028 and the temperature remained constant between the range of its seasonality,and CO_(2)Flux tends to decrease in its seasonality,indicating a higher CO_(2) absorption.

关键词： ARIMA numerical modeling remote sensing orbital products tillage