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Short-term microbial responses to soluble inorganic P input in a tropical lowland rain forest in Amazonia

Pedosphere 2021年第6期31卷 964-972页

作者： Yuri W.L.DE SOUSA Erika BUSCARDO carlos a.n.quesada Henrique E.M.nASCIMEnTO Laszlo nAGY national Research Institute for Amazonia Manaus 69067-375(Brazil) Department of Forestry University of BrasiliaBrasilia 70910-900(Brazil) Centre for Functional Ecology University of CoimbraCoimbra 3000-456(Portugal) Department of Animal Biology University of CampinasCampinas 13083-862(Brazil)

In non-flooded lowland rain forests with low soil phosphorus(P)in parts of Amazonia,P cycling largely occurs via leaf litter recycling by arbuscular mycorrhizal(AM)fungal *** high input of P into these ecosystems occurs during drought years with increased *** the length and frequency of drought events are projected to increase in the region,a single-dose nutrient addition experiment was carried out to test how this would impact P *** application rate of 4 kg P ha^(-1) was used,which corresponds to twice the amount of litter-derived P in an average *** was hypothesized that i)the added mineral P would be immobilized by soil microorganisms,leading to measurable increase in soil microbial biomass carbon(C)and P and ii)AM colonization rate would be reduced by the pulse in mineral P available for plant *** results did not support either of our *** addition of P did not have an effect on AM root colonization,nor was P immobilized by soil microbiota during the experimental *** lack of a difference between the control and treatment at our study site could be attributed to the relatively low one-off dose of P applied that did not change either the colonization rate of roots by AM fungi or the amount of soil available labile *** obtain a mechanistic understanding of the availability,capture,and use of P by plant-symbiont associations in tropical rain forest ecosystems,further integrated studies of the soil-plant system combining long-term nutrient manipulations,modeling,and experimental approaches are required.

关键词： Amazon basin arbuscular mycorrhizal fungi nutrient addition phosphorus cycling soil microbial biomass

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Landform Instability and Land-use Dynamics in Tropical High Mountains, Central Mexico

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Journal of Mountain Science 2012年第3期9卷 414-430页

作者： GARCíA-ROMERO Arturo ACEVES-quesada José Fernando ARREDOnDO-LEón carlos Departamento de Geografía Física Instituto de Geografía Universidad Nacional Autónoma de México. Circuito exterior s/n Ciudad Universitaria 04510 Mexico Distrito Federal Mexico Unidad Académica de Estudios Regionales de la Coordinación de Humanidades Universidad Nacional Autónoma de México

This investigation is an analysis of the influence of landform instability on the distribution of land-use dynamics in a hydrographical basin, located in the Mexican Volcanic Belt mountain range (central Mexico), currently affected by substantial changes in land use and deforestation. A landform map was produced, in addition to seven attribute maps - altimetry, drainage density, slope, relief energy, potential erosion, geology and tectonics - which were considered as factors for determining landform instability through Multi-criteria Evaluation Analysis. Likewise, the direction and rhythm of land-use dynamics were analyzed in four dates - between 1976 and 2000 - and cross tabulations were made between them, in order to analyze the trends and processes of land-use dynamics. Afterwards, the databases obtained were cross tabulated with the landform variables to derive areas, percentages and correlation indices. In the study area, high-instability landforms are associated with most ancient volcanic and sedimentary landforms, where high altitude, drainage density, slope and potential to develop gravitational and fluvial processes are the major factors favouring a land-use pattern, dominated by the conservation of extensive forest land, abandonment of human land use and regeneration of disturbed areas. In contrast, low-instability landforms correspond to alluvial plains and lava hills covered by pyroclasts, where low potential erosion to develop fluvial processes, added to water and soil availability and accessibility, have favoured a land-use pattern dominated by the expansion of agroforestry plantations and human settlements, showing a marked trend towards either intensification or permanence of the current land use and with little abandonment and regeneration.

关键词： Landform instability Multi-criteriaEvaluation Analysis Land-use change and processes Tuxpan River Basin

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Management of gallstones and gallbladder disease in patients undergoing gastric bypass

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World Journal of Gastroenterology 2010年第17期16卷 2075-2079页

作者： Bernabé M quesada Gustavo Kohan Hernán E Roff carlos M Canullán Luis T Chiappetta Porras Department of Surgery Cosme Argerich HospitalAlte.Brown 240ZC 1155 Buenos AiresArgentina

The appropriate management of gallstones and gallbladder disease in patients undergoing gastric bypass remains *** therapeutic modalities are used and include performing cholecystectomy on all patients at the time of gastric bypass,performing concomitant cholecystectomy only when patients have gallstones and performing cholecystectomy only in the presence of both symptoms and *** groups administer ursodeoxycholic acid for gallstone prevention in the postoperative *** treatment modalities are analyzed and their results and rationality are discussed.

关键词： Gallbladder Gastric bypass Morbid obesity Gallstones Cholecystectomy

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Multiphysics Simulation of a novel Concept of MEMS-based Solid Propellant Thruster for Space Propulsion

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Journal of Thermal Science 2011年第6期20卷 527-533页

作者： José A.Moríigo José Hermida-quesada Dept.of Space Programmes National Institute for Aerospace Technology Ctra.Ajalvir km.4Torrejón de Ardoz 28850 MadridSpain Propulsion EngineerPh.D. Dept.of Aerodynamics and Propulsion National Institute for Aerospace Technology Ctra.Ajalvir km.4Torrejón de Ardoz 28850 MadridSpain

This work analyzes a novel MEMS-based architecture of submillimeter size thruster for the propulsion of small spacecrafts,addressing its preliminary characterization of *** architecture of microthruster comprises a setup of miniaturized channels surrounding the solid-propellant reservoir filled up with a high-energetic *** channels guide the hot gases from the combustion region towards the nozzle entrance located at the opposite side of the *** simulations of the transient response of the combustion gases and wafer heating in thruster firings have been conducted with FLUEnT under a multiphysics modelling that fully couples the gas and solid parts *** approach includes the gas-wafer and gas-polymer thermal exchange,burnback of the polymer with a simplified non-reacting gas pyrolysis model at its front,and a slip-model inside the nozzle portion to incorporate the effect of gas-surface and rarefaction onto the gas ***,accurate characterization of thruster operation requires the inclusion of the receding front of the polymer and heat transfer in the moving gas-solid *** study stresses the improvement attained in thermal management by the inclusion of lateral micro-channels in the *** particular,the temperature maps reveal the significant dependence of the thermal loss on the instantaneous surface of the reservoir wall exposed to the heat flux of hot ***,the simulations stress the benefit of implementing such a pattern of micro-channels connecting the exit of the combustion reservoir with the *** results prove that hot gases flowing along the micro-channels exert a sealing action upon the heat flux at the reservoir wall and partly mitigate the overall thermal loss at the inner-wall vicinity during the *** analysis shows that propellant decomposition rate is accelerated due to surface preheating and it suggests that a delay of the flame extinction into the reservoir

关键词： micronozzles spacecraft micropropulsion solid propellant thrusters

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One-oligonucleotide method for constructing vectors for RnA interference

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Acta Pharmacologica Sinica 2005年第12期26卷 1467-1473页

作者： carlosFabianFLORES-JASSO InesVELAZQUEZ-quesada LuisVACA carlosLAnDA-SOLIS AndresAGUTIERREZ Departamento Departamento Departamento Unidad Unidad de Biología Celular Instituto de Fisiología Celular Universidad nacional Autónoma De México Ciudad Universitaria Mexico City 04510 Mexico de Biología Celular Instituto de Fisiología Celular Universidad nacional Autónoma De México Ciudad Universitaria Mexico City 04510 Mexico de Biología Celular Instituto de Fisiología Celular Universidad nacional Autónoma De México Ciudad Universitaria Mexico City 04510 Mexico de Terapia Celular Centro national de Rehabilitación Mexico City 14389 México de Terapia Celular Centro national de Rehabilitación Mexico City 14389 México

Aim:To develop an easy,fast,automated,and inexpensive method for construct-ing short-hairpin-RnA cassettes for RnAi ***:Using singleoligonucleotides,a variety of DnA cassettes for RnAi vectors were constructedin only few minutes in an automated *** cassettes,targeting the eGFP,were cloned into plasmids driven by RnA polymerase Ⅲ promoter ***,theplasmids were transfected into HeLa cells that were later infected with a recombi-nant adenovirus encoding the eGFP *** level of eGFP fluorescence wasevaluated by confocal imaging and flow ***:The plasmids con-structed with the DnA cassettes made by the one-oligonucleotide method inhibi-ted eGFP with different potencies,ranging from 55% to 75%.Conclusion:Byusing the method reported here,it is possible to simultaneously construct hun-dreds of different DnA cassettes for RnAi experiments in an inexpensive,auto-mated *** method will facilitate functional genomics studies on mammaliancells.

关键词： RnA interference shRnAs DnA cassette RnAi vector oligonucleotide

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Healthcare use among people with diabetes mellitus in Europe:a population-based cross-sectional study

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Family Medicine and Community Health 2022年第4期10卷 16-23页

作者： Álvaro Fuentes-Merlos JoséAntonio quesada-Rico Raul Reina Domingo Orozco-Beltrán Faculty of Medicine Miguel Hernandez University of ElcheSant Joan D'AlacantSpain Department of Sports Sciences Sport Research CentreMiguel Hernandez University of ElcheElcheSpain

Objective This study aimed to determine the association of health determinants,lifestyle and socioeconomic variables on healthcare use in people with diabetes in *** A cross-sectional study was conducted using data from the European Health Interview Survey wave 2(ie,secondary analysis).Setting The sample included data from 25 European *** The sample included 16270 patients with diabetes aged 15 years or older(49.1%men and 50.9%women).Results The survey data showed that 58.2%of respondents had seen their primary care physician in the past month and 22.6%had been admitted to the hospital in the past *** of primary care was associated with being retired(prevalence ratio(PR)1.13,95%CI 1.07 to 1.19)and having very poor self-perceived health(PR 1.80,95%CI 1.51 to 2.15),long-standing health problems(PR 1.14,95%CI 1.04 to 1.24),high blood pressure(PR 1.06,95%CI 1.03 to 1.10)and chronic back pain(PR 1.07,95%CI 1.04 to 1.11).Hospital admission was associated with very poor self-perceived health(PR 3.03,95%CI 2.14 to 4.31),accidents at home(PR 1.54,95%CI 1.40 to 1.69),chronic obstructive pulmonary disease(COPD)(PR 1.34,95%CI 1.22 to 1.47),high blood pressure(PR 1.08,95%CI 1.01 to 1.17),chronic back pain(PR 0.91,95%CI 0.84 to 0.98),moderate difficulty walking(PR 1.33,95%CI 1.21 to 1.45)and severe difficulty walking(PR 1.67,95%CI 1.51 to 1.85).Conclusions In the European diabetic population,the high cumulative incidences of primary care visits and hospital admissions are associated with labour status,alcohol consumption,self-perceived health,long-standing health problems,high blood pressure,chronic back pain,accidents at home,COPD and difficulty walking.

关键词： admitted sectional difficulty

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Influence of olive oil and its components on mesenchymal stem cell biology

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World Journal of Stem Cells 2019年第12期11卷 1045-1064页

作者： Antonio Casado-Díaz Gabriel Dorado Jose Manuel quesada-Gómez CIBER de Fragilidad y Envejecimiento Saludable(CIBERFES) Instituto Maimónides de Investigación Biomédica de Córdoba(IMIBIC)Hospital Universitario Reina SofíaCórdoba 14004Spain Departement Bioquímica y Biología Molecular Campus Rabanales C6-1-E17Campus de Excelencia Internacional Agroalimentario(ceiA3)Universidad de CórdobaCIBERFESCórdoba 14071Spain Unidad de Gestión Clínica de Endocrinología y nutrición CIBER de Fragilidad y Envejecimiento Saludable(CIBERFES)Instituto Maimónides de Investigación Biomédica de Córdoba(IMIBIC)Hospital Universitario Reina SofíaCórdoba 14004Spain

Extra virgin olive oil is characterized by its high content of unsaturated fatty acid residues in triglycerides,mainly oleic acid,and the presence of bioactive and antioxidant *** consumption is associated with lower risk of suffering chronic diseases and unwanted processes linked to aging,due to the antioxidant capacity and capability of its components to modulate cellular signaling *** of olive oil can alter the physiology of mesenchymal stem cells(MSCs).This may explain part of the healthy effects of olive oil consumption,such as prevention of unwanted aging *** date,there are no specific studies on the action of olive oil on MSCs,but effects of many components of such food on cell viability and differentiation have been *** objective of this article is to review existing literature on how different compounds of extra virgin olive oil,including residues of fatty acids,vitamins,squalene,triterpenes,pigments and phenols,affect MSC maintenance and differentiation,in order to provide a better understanding of the healthy effects of this ***,most studies have shown a positive effect of these compounds on *** collective findings support the hypothesis that at least part of the beneficial effects of extra virgin olive oil consumption on health may be mediated by its effects on MSCs.

关键词： Olive oil Mesenchymal stem cells Cellular differentiation Aging Cellular niche Mediterranean diet

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Assessing the impact of tropical cyclones on economic sectors in Costa Rica,Central America

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Tropical Cyclone Research and Review 2024年第3期13卷 196-207页

作者： Adolfo quesada-Roman Hugo GHidalgo Eric JAlfaro Laboratorio de Geografía Física Escuela de GeografíaUniversidad de Costa RicaCosta Rica Centro de Investigaciones Geofísicas(CIGEFI) Universidad de Costa RicaCosta Rica Escuela de Física Universidad de Costa RicaCosta Rica Centro de Investigacion en Matematica Pura y Aplicada(CIMPA) Universidad de Costa RicaCosta Rica Centro de Investigacion en Ciencias del Mar y Limnología(CIMAR) Universidad de Costa RicaCosta Rica

Tropical cyclones(TC)pose a persistent natural hazard to Costa *** to natural hazards,such as mass movements and floods,is compounded by a growing urban population and inadequate land use *** study conducted a comprehensive analysis of the economic impacts of TC of Costa Rica from Hurricane Joan in 1988 to Hurricane Eta in 2020,assessing the impact by municipality and economic sector using baseline information of the Ministry of national Planning and Economic *** to the study,road infrastructure(933.8 US million),agriculture(280.5 US million),river rehabilitation(153.96 US million),housing 98.26(US million),and health(81.74 US million)were among the sectors most severely affected by TC over the past 30 *** Pacific basin municipalities in Costa Rica were found to be the most vulnerable,primarily due to the indirect impacts of *** study's results offer useful information on the economic sectors and municipalities that are most exposed from TC in Costa Rica and provide a replicable methodology for other regions and countries facing similar tropical phenomena.

关键词： Tropical cyclones natural hazards Economic impacts Municipality impact Developing countries Central America

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Increased incidence of and microbiologic changes in pyogenic liver abscesses in the Mexican population

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World Journal of Hepatology 2020年第10期12卷 816-828页

作者： Juanita Pérez-Escobar Wagner Ramirez-quesada Daniel Alejandro Calle-Rodas Luis Alberto Chi-Cervera nalu navarro-Alvarez Jorge Aquino-Matus Juan Pablo Ramírez-Hinojosa carlos Moctezuma-Velázquez Aldo Torre Department of Gastroenterology Instituto Nacional de Ciencias Médicas y Nutrición Salvador ZubiránMexico City 14080Mexico Department of Internal Medicine Hospital General Dr.Manuel Gea GonzálezMexico City 14080Mexico

BACKGROUnD Pyogenic liver abscess(PLA)is a rare disease with an estimated incidence that varies widely across the globe,being as high as 115.4/100000 habitants in Taiwan and as low as 1.1-1.2/100000 habitants in Europe and *** though there are multiple microorganisms capable of producing an abscess in the liver,including Entamoeba histolytica,fungi,and viruses,most abscesses are derived from bacterial *** epidemiology of PLA in Mexico is currently *** To describe the clinical,demographic and microbiologic characteristics of PLA in *** This is a retrospective study carried out in two centers,and included patients seen between 2006 and 2018 with the diagnosis of pyogenic *** collected demographic,clinical,and microbiological information,treatment,complications,and outcomes.A logistic regression analysis was used to determine the association between different variables and mortality *** A total of 345 patients were included in this study.233(67.5%)had confirmed PLA,133(30%)patients had no positive culture and negative serology and 9(2.5%)had mixed *** mean age was 50 years(ranging from 16-97 years)and 63%were female.65%of the patients had positive cultures for Extended Spectrum Beta-Lactamases(ESBL)-Escherichia coli and Klebsiella *** was administered in 60%of *** most common sources of infection were ascending cholangitis and cholecystitis in 34(10%)and 31(9%),*** median length of hospital stay was 14 d.165 patients underwent percutaneous catheter *** inpatient mortality rate was 63%.Immunocompromised state[OR 3.9,95%CI:1.42-10.46],ESBL-Escherichia coli[OR 6.7,95%CI:2.7-16.2]and Klebsiella pneumoniae[OR 4-8,95%CI:1.6-14.4]predicted inpatient mortality by multivariate *** The prevalence of PLA is increasing in Mexico and has a very high mortality ***-Escherichia coli and Klebsiella pneumoniae are the most common microorganisms causing PLA

关键词： Liver abscess Pyogenic Mexican population Epidemiology Complications Outcomes Mortality

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Predicting the optical observables for nucleon scattering on even-even actinides

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Chinese Physics C 2017年第9期41卷 123-128页

作者： D. S. Martyanov E. Sh. Soukhovitskii R. Capote J. M. quesada S. Chiba Joint Institute for Energy and nuclear Research 220109 Minsk-Sosny Belarus nAPC-nuclear Data Section International Atomic Energy Agency Vienna A-1400 Austria Departamento de Fisica Atomica Molecular y Nuclear Universidad de Seviha Ap.1065 E-4i080 Sevilla Spain Research Laboratory for nuclear Reactors Tokyo Institute of Technology 2-12-1-N1-90okuyama Meguro-ku Tokyo 152-8550 Japan

The previously derived Lane consistent dispersive coupled-channel optical model for nucleon scattering on 232Th and 23Su nuclei is extended to describe scattering on even-even actinides with Z=90-98. A soft-rotator- model （SRM） description of the low-lying nuclear structure is used, where the SRM Hamiltonian parameters are adjusted to the observed collective levels of the target nucleus. SRM nuclear wave functions （mixed in K quantum number） have been used to calculate the coupling matrix elements of the generalized optical model. The ＂effective＂ deformations that define inter-band couplings are derived from the SRM Hamiltonian parameters. Conservation of nuclear volume is enforced by introducing a dynamic monopolar term to the deformed potential, leading to additional couplings between rotational bands. The fitted static deformation parameters are in very good agreement with those derived by Wang and collaborators using the Weizs^cker-Skyrme global mass model （WS4）, allowing use of the latter to predict cross sections for nuclei without experimental data. A good description of the scarce ＂optical＇experimental database is achieved. SRM couplings and volume conservation allow a precise calculation of the compound-nucleus formation cross sections, which is significantly different from that calculated with rigid-rotor potentials coupling the ground-state rotational band. The derived parameters can be used to describe both neutron- and proton-induced reactions.

关键词： optical model soft-rotator model FRDM deformations WS4 deformations even-even actinides

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