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2型猪链球菌细胞分裂蛋白GpsB的原核表达及其鉴定

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中国人兽共患病学报 2019年第3期35卷 201-205页

作者：侯红芬张会芳胡丹郑峰朱旭辉王长军潘秀珍曹祥荣南京师范大学生命科学学院南京210046 南京军事医学研究所南京210002

目的利用原核表达系统诱导表达2型猪链球菌(Streptococcus suis 2,*** 2)分裂相关因子GpsB重组蛋白,为后续研究奠定基础。方法以*** 2菌株05ZYH33全基因组DNA为模板,经PCR扩增得到目的基因片段。目的基因经双酶切后连接至表达载体pET32a... 详细信息

目的利用原核表达系统诱导表达2型猪链球菌(Streptococcus suis 2,*** 2)分裂相关因子GpsB重组蛋白,为后续研究奠定基础。方法以*** 2菌株05ZYH33全基因组DNA为模板,经PCR扩增得到目的基因片段。目的基因经双酶切后连接至表达载体pET32a,转化大肠杆菌(Escherichia coli,***)DH5α感受态细胞。重组质粒经测序鉴定正确后转化*** BL21感受态细胞。获得的重组表达菌经IPTG诱导表达目的蛋白。利用Ni离子亲和层析柱纯化重组蛋白,并进行SDS-PAGE和Western blot鉴定。利用重组蛋白免疫小鼠制备多克隆抗体。结果成功构建出重组表达载体pET32a^(gpsB),并经IPTG诱导表达出目的蛋白。重组蛋白主要存在于表达菌裂解液上清中,分子质量约30 kD,与预期大小一致。Western blot检测发现,该蛋白能被His-Tag单克隆抗体特异性识别。制备的多克隆抗体能特异性识别重组GpsB蛋白(rGpsB)。结论成功表达和纯化了rGpsB并获得了该蛋白的多克隆抗体,为进一步研究该蛋白在*** 2分裂过程中的作用鉴定了基础。

关键词： 2型猪链球菌 GpsB 原核表达多克隆抗体

2型猪链球菌酪氨酸激酶基因的原核表达及表达产物抗血清的制备

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中国病原生物学杂志 2020年第2期15卷 136-140页

作者：张会芳吴倩倩王悄悄王晶胡丹郑峰汪春晖潘秀珍曹祥荣南京师范大学生命科学学院江苏南京210023 东部战区疾病预防控制中心南京医科大学附属无锡妇幼保健院

目的制备2型猪链球菌(Streptococcus suis 2)酪氨酸激酶Cps2C重组蛋白及其抗血清,为进一步研究Cps2C的作用奠定基础。方法采用在线工具对cps2C基因进行生物信息学分析。设计cps2C基因引物,以05ZYH33菌株基因组DNA为模板PCR扩增cps2C基因... 详细信息

目的制备2型猪链球菌(Streptococcus suis 2)酪氨酸激酶Cps2C重组蛋白及其抗血清,为进一步研究Cps2C的作用奠定基础。方法采用在线工具对cps2C基因进行生物信息学分析。设计cps2C基因引物,以05ZYH33菌株基因组DNA为模板PCR扩增cps2C基因,构建pET32a:cps2C重组质粒;重组质粒转入感受态*** BL21后,IPTG诱导表达Cps2C重组蛋白,经Ni2+亲和层析纯化后用His单克隆抗体进行鉴定。用纯化的重组蛋白免疫BALB/c小鼠,获得多克隆抗血清,采用间接ELISA法测定抗体效价。结果成功构建pET32a:cps2C表达质粒,获得阳性*** BL21Cps2C重组蛋白表达菌株,SDS-PAGE显示纯化的目的蛋白条带单一,相对分子质量为43×103,与预期一致;Western blot检测Cps2C重组蛋白可以与His单克隆抗体、感染05ZYH33全菌的猪恢复期血清反应。制备的多克隆抗血清效价为1∶819 200,且能与重组蛋白反应。结论原核表达了具有抗原性的Cps2C重组蛋白,制备的鼠源多抗血清能识别Cps2C蛋白,为研究Cps2C蛋白在2型猪链球菌中的作用奠定了基础。

关键词： 2型猪链球菌原核表达酪氨酸激酶多抗血清

Effect of Boron Mud and CaF_2 on Surface Tension and Density of cao-SiO_2-B_2O_3 Ternary Slag Systems

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Journal of Iron and Steel Research International 2014年第8期21卷 745-748页

作者： Li-li YANG Hong-ming WANG xiang ZHU Gui-rong LI School of Materials Science and Engineering Jiangsu University Jiangsu Province Key Laboratory of High-End Structural Materials

The effect of boron mud and CaF2,which were used as fluxing agents,on surface tension and density of cao-SiO2-B2O3 ternary slag systems was *** surface tension and density were measured by using the ring detachment method and Archimedean method,*** results indicated that surface tension and density of the slag systems decreased with increasing temperature in the range of 1380-1480℃.When 5%-10% CaF2 was employed as fluxing agent,the surface tension and density of slag were decreased with increasing CaF2 content,and the surface tension changed from 0.3to 0.6N/m,while the density changed from 3.4to 4.1g/***,when using 5%-10%boron mud as fluxing agent,the surface tension was enhanced,whereas the density decreased with the increase of boron mud content,and the variation range was 0.4-0.6N/m for surface tension and 3.2-3.9g/cm3 for the *** a conclusion,using boron mud as fluxing agent to substitute for CaF2 did not cause significant changes in slag surface tension and density.

关键词： boron mud calcium fluoride surface tension density

Replacing PVP by macrocycle cucurbit[6]uril to cap sub-5 nm Pd nanocubes as highly active and durable catalyst for ethanol electrooxidation

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Nano Research 2019年第10期12卷 2628-2633页

作者： Dongshuang Wu Minna cao rong cao State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of MatterChinese Academy of SciencesFuzhou 350002China

Pd nano cubes(NCs)en closed by six{100}facets are fasci nating model materials for both fun dame ntal studies and practical applicati ***,the only available method to prepare well-defined sub-10 nm Pd NCs was developed by Xia et *** than 10 years ago,un avoidably using polyvinyl pyrrol id one(PVP)polymer to preve nt particle aggregati *** strongly adsorbed PVP extremely deteriorates the catalysts*efficiency because of the high coverage of accessible surface-active *** efforts have been devoted to replacing PVP with weaker capping agents but with limited progress predominately due to the difficulties in tuning the growth kinetics of Pd *** the first time,we report that macrocycle cucurbit[6]uril(CB[6])can replace PVP in the synthesis of Pd NCs by dedicatedly controlling the growth ***[6]capped Pd NCs showed 1.1-1.5 times in creased specific surface area compared to the surfactant-free commercial Pd ***,X-ray photoelectron spectroscopy dem on strated the modified electronic structure of Pd NCs through the carb onyl group of CB[6].Consequently,compared to the commercial catalysts,the obtained Pd NCs exhibited 7 times higher current density towards ethanol oxidation ***,after 17 h of continuous work,it reduced deactivation by up to 1-4 orders of magnitude.

关键词： palladium cucurbituril nanocubes ethanol oxidation fuel cells

血管生成靶向纳米粒子c(RGDyK)@SiO_2@Fe_3O_4的合成及生物性能

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中国组织工程研究与临床康复 2012年第3期16卷 413-416页

作者：孙鹏飞曹向荣朱浩焦宗宪张锋伟颉克蓉兰州大学第二医院甘肃省兰州市730030 兰州大学基础医学院甘肃省兰州市730000 化学化工学院甘肃省兰州市730000

背景:SiO2含有较多的羟基官能团,可进一步功能化而与靶向性配体相偶联,从而拓展Fe3O4@SiO2纳米粒子在生物医药领域的应用。目的:探讨靶向性纳米粒子c(RGDyK)@SiO2@Fe3O4)的合成方法,并对其性能进行测试。方法:采用一壶化学共沉淀法合成... 详细信息

背景:SiO2含有较多的羟基官能团,可进一步功能化而与靶向性配体相偶联,从而拓展Fe3O4@SiO2纳米粒子在生物医药领域的应用。目的:探讨靶向性纳米粒子c(RGDyK)@SiO2@Fe3O4)的合成方法,并对其性能进行测试。方法:采用一壶化学共沉淀法合成油酸修饰的疏水性Fe3O4纳米粒子,采用反相微乳液法合成生物相容性Fe3O4@SiO2复合纳米粒子;以3-氨丙基三乙氧基硅烷为偶联剂将复合粒子中SiO2表面的羟基氨基化、醛基化,加入1.0mgc(RGDyK)多肽,超声震荡下反应生成c(RGDyK)@SiO2@Fe3O4纳米粒子。将Fe3O4@SiO2或c(RGDyK)@SiO2@Fe3O4与人脐静脉细胞融合细胞(***926)共培养24,48,72h进行检测。结果与结论:实验合成的Fe3O4@SiO2复合纳米粒子的平均粒径为40nm,应用3-氨丙基三乙氧基硅烷可将c(RGDyK)成功耦合于复合粒子的SiO2表面。Fe3O4@SiO2或c(RGDyK)@SiO2@Fe3O4与***926共培养24h,***926细胞活性明显增高(P0.05)。电镜观察发现,***926细胞对靶向性c(RGDyK)@SiO2@Fe3O4粒子的吞噬能力明显强于非靶向性Fe3O4@SiO2粒子。说明实验合成的c(RGDyK)@SiO2@Fe3O4纳米粒子具有良好的生物相容性、超顺磁性及较高的血管内皮细胞靶向性,是一种优良的生物材料。

关键词：微乳液法二氧化硅四氧化三铁四甲基偶氮唑盐 RGD肽生物相容性超顺磁性靶向性

64层螺旋CT低剂量管电流恒定技术在胸部的临床应用研究

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兰州大学学报（医学版） 2014年第4期40卷 57-62页

作者：马来阳孙鹏飞曹向荣何慧董永兴叶斌强邓荣庭兰州大学第二医院放射科甘肃兰州730030

目的探讨64层螺旋CT低剂量管电流恒定技术(CCC)在胸部扫描中的价值。方法 80例受试者随机分为4组,分别为C1组(50 m A)、C2组(30 m A)、C3组(20 m A)和A组,C1-C3组采用CCC扫描,A组采用自动曝光控制技术(AEC)扫描作为对照。扫描结束后分... 详细信息

目的探讨64层螺旋CT低剂量管电流恒定技术(CCC)在胸部扫描中的价值。方法 80例受试者随机分为4组,分别为C1组(50 m A)、C2组(30 m A)、C3组(20 m A)和A组,C1-C3组采用CCC扫描,A组采用自动曝光控制技术(AEC)扫描作为对照。扫描结束后分析不同组横断面图像质量评分、图像噪声以及剂量长度乘积,以确定最佳的恒定管电流扫描方案。结果 CCC扫描各组和AEC扫描图像质量比较差异无统计学意义(P>0.05);CCC扫描各组间所得图像质量比较差异也均无统计学意义(P>0.05)。C2组和C3组图像噪声显著高于AEC扫描(PC2组>C1组,差异有统计学意义(P<0.05)。CCC扫描各组辐射剂量明显低于AEC扫描(P<0.05),且C3组剂量最低。结论 CCC扫描各组图像质量良好且辐射剂量明显低于AEC扫描,64层螺旋CT低剂量(20 m A)扫描可应用于胸部常规检查。

关键词：低剂量胸部管电流恒定技术体层摄影术 X线计算机

Evolution of nuclear charge radii in copper and indium isotopes

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Chinese Physics C 2022年第6期46卷 138-146页

作者： rong An xiang Jiang Li-Gang cao Feng-Shou Zhang Key Laboratory of Beam Technology of Ministry of Education Institute of Radiation TechnologyBeijing Academy of Science and TechnologyBeijing 100875China Key Laboratory of Beam Technology of Ministry of Education College of Nuclear Science and TechnologyBeijing Normal UniversityBeijing 100875China College of Physics and Optoelectronic Engineering Shenzhen UniversityShenzhen 518060China Center of Theoretical Nuclear Physics National Laboratory of Heavy Ion Acceleator of LanzhouLanzhou 730000China

Systematic trends in nuclear charge radii are of great interest due to universal shell effects and odd-even staggering(OES).The modified root mean square(rms)charge radius formula,which phenomenologically accounts for the formation of neutron-proton(np)correlations,is here applied for the first time to the study of odd-Z copper and indium *** results obtained by the relativistic mean field(RMF)model with NL3,PK1 and NL3^(*)parameter sets are compared with experimental *** results show that both OES and the abrupt changes across N=50 and 82 shell closures are clearly reproduced in nuclear charge *** inverted parabolic-like behaviors of rms charge radii can also be described remarkably well between two neutron magic numbers,namely N=28 to 50 for copper isotopes and N=50 to 82 for indium *** implies that the np-correlations play an indispensable role in quantitatively determining the fine structures of nuclear charge radii along odd-Z isotopic ***,our conclusions have almost no dependence on the effective forces.

关键词： nuclear charge radii neutron-proton correlations relativistic mean field theory odd-even staggering shell closure

Design and numerical analysis of serpentine microchannel integrated with inner-wall ridges for enhanced droplet mixing

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Science China(Technological Sciences) 2023年第2期66卷 560-573页

作者： cao xiang CHEN YongPing Key Laboratory of Energy Thermal Conversion and Control of Ministry of Education School of Energy and EnvironmentSoutheast UniversityNanjing 210096China Jiangsu Key Laboratory of Micro and Nano Heat Fluid Flow Technology and Energy Application School of Environmental Science and EngineeringSuzhou University of Science and TechnologySuzhou 215009China

A numerical model is developed for three-dimensional droplet mixing in serpentine microchannels with inner-wall ridges(referred to as SMR microchannels).The underlying mechanisms of mixing in SMR microchannels are revealed,and the effects of the ridge width and ridge number on mixing inside droplets of different sizes are *** results indicate that SMR microchannels are capable of enhancing the mixing efficiency by 3.6%–12.5%compared with that in smooth serpentine microchannels,particularly when the droplet length is larger than the microchannel width and the width ratio of the ridge to the microchannel is 0.25–*** above mixing enhancement is determined by two main factors:the combined effects of the vortex pair and reverse flow,and non-horizontal ***,when the width ratio is 1,there is a maximum 16.0%increase in mixing efficiency for small droplets with a length equal to the microchannel ***,for small droplets,mixing in an SMR microchannel is worse than in a smooth serpentine microchannel when the width ratio is less than *** increasing the ridge number,when the width ratio is 0.25,the mixing efficiency is reduced for small droplets but increased for larger droplets.

关键词： droplet mixing serpentine microchannel ridge microfluidics

First-Principles Study of Structural,Elastic and Electronic Properties of OsSi

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Communications in Theoretical Physics 2009年第10期52卷 701-706页

作者： LI Jin LINGHU rong-Feng YANG Ze-Jin cao Yang YANG xiang-Dong Institute of Atomic and Molecular Physics of Sichuan University Chengdu 610065 China College of Material and Chemical Engineering Hainan University Haikou 570228 China Hainan Provincial Key Laboratory of Research on Utilization of Si-Zr-Ti Resources Haikou 570228 China School of Physics and Electronic Science Guizhou Normal University Guiyang 550001 China

First-principles study of structural, elastic, and electronic properties of the B20 structure OsSi has been reported using the plane-wave pseudopotential density functional theory method. The calculated equilibrium lattice and elastic constants are in good agreement with the experimented data and other theoretical results. The dependence of the elastic constants, the aggregate elastic modulus, the deviation from the Cauchy relation, the elastic wave velocities in different directions and the elastic anisotropy on pressure have been obtained and discussed. This could be the first quantitative theoretical prediction of the elastic properties under high pressure of OsSi compound. Moreover, the electronic structure calculations show that OsSi is a degenerate semiconductor with the gap value of 0.68 eV, which is higher than the experimental value of 0.26 eV. The analysis of the PDOS reveals that hybridization between Os d and Sip states indicates a certain covalency of the Os-Si bonds.

关键词： OsSi elastic constants electronic properties high pressure effect