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Rapid detection of Pseudomonas aeruginosa by cross priming amplification

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Journal of Integrative Agriculture 2020年第10期19卷 2523-2529页

作者： XIANG Yong YAN Ling ZHENG Xiao-cui LI Li-zhen LIU Peng cao wei-sheng College of Veterinary Medicine South China Agricultural UniversityGuangzhou 510642P.R.China Key Laboratory of Zoonosis Prevention and Control of Guangdong Province South China Agricultural UniversityGuangzhou 510642P.R.China National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control South China Agricultural UniversityGuangzhou 510642P.R.China South China Collaborative Innovation Centre for Prevention and Control of Poultry Infectious Diseases and Safety of Poultry Products South China Agricultural UniversityGuangzhou 510642.P.R.China

Pseudomonas aeruginosa(PA)is an opportunistic pathogen of humans and animals and a common source of nosocomial infections especially of the respiratory *** aeruginosa is also a major bacterial disease of poultry and in particular,eggs and newly hatched *** this study,we developed a simple,accurate and rapid molecular detection method using cross priming amplification(CPA)with a nucleic acid test strip to detect *** assay efficiently amplified the target gene within 45 min at 62℃only using a simple water *** detection limit of the method was 1.18x 102 copiesμL^-1 for plasmid DNA and 4.4 CFU mL^-1 for bacteria in pure culture,and was 100 times more sensitive than conventional *** screened 83 clinical samples from yellow-feather broiler breeder chickens and hospitalized/treated dogs and cats using CPA,PCR and traditional culture *** positive sample ratios were 15.3%(13/83)by CPA,13.3%(11/83)by PCR and 12.1%(10/83)by the culture *** established CPA method has significant advantages for detecting *** method is easy to use and possesses high specificity and sensitivity without the requirements of complicated experimental *** PA-CPA assay is especially fit for outdoor and primary medical units and is an ideal system for the rapid detection and monitoring of ***.

关键词： Pseudomonas aeruginosa cross priming amplification isothermal amplification rapid detection detection method

Modified cellular automaton model for modeling of microstructure and microsegregation in solidification of ternary alloys

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中国有色金属学会会刊：英文版 2006年第A2期16卷 180-185页

作者：朱鸣芳 cao wei-sheng CHEN Shuang-lin XIE Fan-you HONG Chun-pyo CHANG Y. Austin School of Materials Science and Engineering Southeast University Nanjing 210096 China Department of Materials Science and Engineering University of Wisconsin-Madison 1509 University Avenue Madison WI 53706 USA CompuTherm LLC 437 S. Yellowstone Dr. Suite 217 Madison WI 53719 USA Center for Computer-Aided Materials Processing （CAMP） Department of Metallurgical Engineering Yonsei University Shinchon-dong 134 Seodaemun-ku Seoul 120-749 Korea

A modified cellular automaton (MCA) model has been extended to the ternary alloy system by coupling thermodynamic and phase equilibrium calculation engine PanEngine. In the present model the dendrite growth is driven by the difference between the local equilibrium liquidus temperature and local actual temperature, incorporating the effect of curvature. The local equilibrium liquidus temperature is calculated with PanEngine according to the local liquid concentrations of two solutes, which are determined by numerically solving the species transport equation in the domain. Model validation was carried out through the comparison of the simulated values to the prediction of the Scheil model for solute profiles in the primary dendrites. The simulated data with zero solid diffusivity and limited liquid diffusivity were increasingly close to the Scheil profiles as the solidification rate decreased. The simulated microstructure and microsegregation in an Al-Cu-Mg ternary alloy were compared with those obtained experimentally.

关键词：三元合金体系凝固显微结构模型显微偏析细胞自动机

Isolation and the Full-length Genome Sequencing of Subgroup J Avian Leukosis Virus ZH-08 Isolate Associated with Hemangioma

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畜牧兽医学报 2011年第B12期42卷 51-56页

作者： ZHANG Xiao-tao SHI wei-wei LIU Hong-bo ZHANG He-nan LIAO Ming XIN Chao-an cao wei-sheng （Key Laboratory of Animal Disease Control and Prevention of the Ministry of Agriculture College of Veterinary Medicine South China Agricultural University Guangzhou 510642 China）

A subgroup J avian leukosis virus (AVL-J), designated as ZH-08, was isolated from a breeder flock in Guangdong province with a novel hemangioma case. The identification results of ELISA test, PCR and immunofluoresecence assay (IFA) specific for ALV-J were all positive. Based on the public full-length proviral genome sequence of ALV-J prototype strain HPRS-103, three pairs of primers were synthesized. The full-length proviral genome sequence of ZH-08 isolate is 7597 bp, which has a little difference with that of published full-length genome sequences, but its organization corresponds with typical retroviral genome structure; and known oncogenes were not included in its genome. According to the gp85 sequence comparison of ZH-08 isolate with those of the other reference strains in China and abroad, the highest similarity (93.7%) was with the YZ9901 isolate. Phylogenetic analysis, based on the gp85 gene, showed that the ZH-08 isolated here had the closest linkage to the SD07LK1 isolate. This study provides the basis for the biological characterization and pathogenesis research of the ZH-08 isolate.

关键词： J亚群禽白血病病毒全基因组测序血管全基因组序列酶联免疫吸附试验 ALV-J 聚合酶链反应 gp85基因

Boosting Oxygen Evolution Reaction Performance on NiFe‑Based Catalysts Through d‑Orbital Hybridization

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Nano-Micro Letters 2025年第1期17卷 281-292页

作者： Xing Wang wei Pi sheng Hu Haifeng Bao Na Yao wei Luo State Key Laboratory of New Textile Materials and Advanced Processing Technology Key Laboratory of New Textile Materials and Applications of Hubei ProvinceSchool of Materials Science and EngineeringWuhan Textile UniversityWuhan 430200People’s Republic of China College of Chemistry and Molecular Sciences Wuhan UniversityWuhan 430072People’s Republic of China

Anion-exchange membrane water electrolyzers(AEMWEs)for green hydrogen production have received intensive attention due to their feasibility of using earth-abundant NiFe-based *** introducing a third metal into NiFe-based catalysts to construct asymmetrical M-NiFe units,the d-orbital and electronic structures can be adjusted,which is an important strategy to achieve sufficient oxygen evolution reaction(OER)performance in ***,the ternary NiFeM(M:La,Mo)catalysts featured with distinct M-NiFe units and varying d-orbitals are reported in this *** and theoretical calculation results reveal that the doping of La leads to optimized hybridization between d orbital in NiFeM and 2p in oxygen,resulting in enhanced adsorption strength of oxygen intermediates,and reduced rate-determining step energy barrier,which is responsible for the enhanced OER *** critically,the obtained NiFeLa catalyst only requires 1.58 V to reach 1 A cm^(−2) in an anion exchange membrane electrolyzer and demonstrates excellent long-term stability of up to 600 h.

关键词： NiFe-based catalysts d-orbital coupling Oxygen evolution reaction Anion exchange membrane electrolyzer

Si-C Foam Porous-Medium Combustion Power-Generation System for Low-Calorific-Value Biomass Syngas

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Journal of Thermal Science 2025年第1期34卷 206-222页

作者： YANG Jianwen CHEN wei cao Bingwei LIU Xinhui LI Hang College of Mechanical and Aerospace Engineering Jilin University School of Mechanical and Vehicle Engineering Changchun University

Syngas produced from the gasification of organic feedstocks from biomass is one of the clean and sustainable sources of *** advantages of simple access and renewability of biomass energy can meet the energy needs of temporary power *** study presents a biomass power-generation system for vehicular *** biomass and a free-piston Stirling engine generator（FPSEG） as the primary material and prime mover,respectively,biomass energy is converted into electricity by combusting the syngas to heat the *** and key parameter design for biomass gasification and thermoelectric conversion systems within a power generation system were performed.A porous medium area was constructed using Si-C foam ceramics to obtain an energy-conversion experimental *** effects of bed height,porosity,porous-region diameter,and air-intake conditions on the power-generation performance were investigated,and optimisations were performed for the thermoelectric conversion *** rate of increase during FPSEG power generation first increased and then decreased with increasing bed height,peaking at a bed height of 40 *** increasing porous-region diameter accelerated FPSEG power generation,whereas porosity changes in the porous media did not significantly affect the rate of change during FPSEG power *** increasing air intake,the rate of increase during power generation first increased and then *** maximum change rate and the highest thermoelectric conversion efficiency of the power-generation system occurred at 9.5 m3/h and 6.5 m3/h（～45.1%）air intakes,*** the thermal inertia and combustion structure of the thermoelectric conversion system significantly increased the power-generation rate of the system,with 1.8 W/s being observed at a 9.7 m3/h air intake.

关键词： biomass combined heat and power (CHP) gasification porous media combustion power generation

Melting Evaluation of Phase Change Materials Impregnated into Cascaded Metal Foams with Regionalized Enhancement

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Journal of Thermal Science 2025年第1期34卷 223-241页

作者： wei Xinyi ZHANG Nan ZHANG Zhaoli cao Xiaoling YUAN Yanping School of Mechanical Engineering Southwest Jiaotong University

Metal foam promotes the heat transfer of phase change materials(PCMs) in the penalty of reducing the energy storage density of the composite *** this work,the effects of constant porosity(0.96,0.94,0.92,or 0.90) and pore density(PPI) of metal foam on heat transfer of composite PCMs are *** rate could be enhanced by employing with low porosity copper ***,aiming to the right bottom phase changing "dead region",a regionalized enhancement strategy of cascaded metal foams is *** dynamic melting performances of all the composite PCMs are comprehensively *** results reveal that the cascaded configuration is beneficial for *** show that the horizontal strategy enhances melting performance:a maximum of 17.98% reduction in total melting time could be reached when the rear part porosity is 90%.The energy storage density rate could be raised by 5.48%.Besides,the vertical strategy performs with a better average temperature uniformity of 0.441 and brings a lower temperature in the heated *** sum up,the regionalized enhancement of copper foam provides better performance in the phase change *** shows significant potential for solar heat storage and thermal protection.

关键词： copper metal foam heat transfer enhancement phase change materials regionalized enhancement

MXene Hybridized Polymer with Enhanced Electromagnetic Energy Harvest for Sensitized Microwave Actuation and Self‑Powered Motion Sensing

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Nano-Micro Letters 2025年第3期17卷 265-279页

作者： Yu‑Ze Wang Yu‑Chang Wang Ting‑Ting Liu Quan‑Liang Zhao Chen‑Sha Li Mao‑sheng cao School of Materials Science and Engineering Beijing Institute of TechnologyBeijing 100081People’s Republic of China School of Materials Science and Engineering Peking UniversityBeijing 100871People’s Republic of China School of Mechanical and Material Engineering North China University of TechnologyBeijing 100144People’s Republic of China Key Laboratory of Functional Inorganic Material Chemistry Ministry of Education of the People’s Republic of ChinaHeilongjiang UniversityHarbin 150080People’s Republic of China

Polymeric microwave actuators combining tissue-like softness with programmablemicrowave-responsive deformation hold great promise for mobile intelligentdevices and bionic soft robots. However, their application is challenged by restricted electromagneticsensitivity and intricate sensing coupling. In this study, a sensitized polymericmicrowave actuator is fabricated by hybridizing a liquid crystal polymer with Ti3C2Tx(MXene). Compared to the initial counterpart, the hybrid polymer exhibits unique spacechargepolarization and interfacial polarization, resulting in significant improvements of230% in the dielectric loss factor and 830% in the apparent efficiency of electromagneticenergy harvest. The sensitized microwave actuation demonstrates as the shortenedresponse time of nearly 10 s, which is merely 13% of that for the initial shape memory polymer. Moreover, the ultra-low content of MXene (upto 0.15 wt%) benefits for maintaining the actuation potential of the hybrid polymer. An innovative self-powered sensing prototype that combinesdriving and piezoelectric polymers is developed, which generates real-time electric potential feedback (open-circuit potential of ~ 3 mV) duringactuation. The polarization-dominant energy conversion mechanism observed in the MXene-polymer hybrid structure furnishes a new approachfor developing efficient electromagnetic dissipative structures and shows potential for advancing polymeric electromagnetic intelligent devices.

关键词： Microwave absorption Electromagnetic response Energy harvest Self-sensing Soft actuator

黄河源区多年冻土活动层土壤水文过程季节变异分析

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水科学进展 2018年第1期29卷 1-10页

作者：曹伟盛煜吴吉春王生廷马帅中国科学院西北生态环境资源研究院冻土工程国家重点实验室甘肃兰州730000

为从整体上认识多年冻土活动层土壤水文过程季节变异特性,以黄河源区巴颜喀拉山北坡冻土剖面为例,结合大气降水、冻土土壤水分、冻土层上水的野外观测,采用HYDRUS-1D软件冻融模块进行模拟分析,分析冻融作用对活动层土壤水文过程的影响,... 详细信息

为从整体上认识多年冻土活动层土壤水文过程季节变异特性,以黄河源区巴颜喀拉山北坡冻土剖面为例,结合大气降水、冻土土壤水分、冻土层上水的野外观测,采用HYDRUS-1D软件冻融模块进行模拟分析,分析冻融作用对活动层土壤水文过程的影响,研究结果表明:(1)冻土层上水位与土壤水热之间存在着相互影响、相互作用的关系,依据活动层土壤温度变化,基于冻融过程,多年冻土活动层土壤水分与冻土层上水位可划分为冻结稳定、快速融化、融化稳定和快速冻结4个阶段。(2)降雨入渗是坡面尺度下活动层土壤水文过程的主要驱动力,活动层冻融锋面是主要限制性因素,受冻融过程影响,冻结期降雨减少,土壤冻结,土壤储水能力下降,土壤水分下渗停止,坡面侧向流动减弱,土壤水分和冻土层上水位处于下降趋势;融化期降雨增多,土壤融化,土壤储水能力上升,土壤水分下渗强烈,坡面侧向流动增强,土壤水分和冻土层上水位处于上升趋势。(3)受坡面地形影响,上坡活动层厚度大于下坡,上坡冻融锋面变化较下坡平缓,上坡土壤水分和冻土层上水位的变化幅度相对下坡较为平缓,而上坡土壤水分相对下坡含量较低,下坡冻土层上水位相对稳定。

关键词：多年冻土活动层土壤水文过程季节变异黄河源区

江汉盆地潜江组与沙市组盐间非砂岩油气藏勘探潜力对比

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石油勘探与开发 2007年第2期34卷 190-196页

作者：陈波肖秋苟曹卫生赵海涛长江大学地球科学学院中国石化中南分公司中国石化江汉油田分公司勘探开发研究院

在江汉盆地潜江凹陷潜江组发育盐间非砂岩油气藏。江陵凹陷沙市组也沉积了一套含盐地层,但盐间非砂岩油气藏发育程度不如潜江组。沉积特征、成藏条件和成藏机制研究发现,两套含盐地层及其盐韵律特征的差异受构造环境、古气候环境控制。... 详细信息

在江汉盆地潜江凹陷潜江组发育盐间非砂岩油气藏。江陵凹陷沙市组也沉积了一套含盐地层,但盐间非砂岩油气藏发育程度不如潜江组。沉积特征、成藏条件和成藏机制研究发现,两套含盐地层及其盐韵律特征的差异受构造环境、古气候环境控制。潜江组沉积时期,断陷活动强,形成补偿或欠补偿的深水沉积环境,利于有机质堆积,盐韵律特征由纯盐岩层和盐间深灰色泥岩、油页岩、含芒硝白云岩和白云岩等组成,深灰色泥岩、油页岩为有利烃源岩,含芒硝白云岩和白云岩等为有利储集层,具备形成盐间非砂岩油气藏的条件。沙市组沉积时期,断陷活动弱,气候干旱,形成补偿到过补偿的沉积环境,盐韵律由含膏盐岩、膏质盐岩和盐间紫色、灰色泥岩或含芒硝泥岩组成,烃源岩和储集层条件较差,但其下段上部的一套灰色盐岩、膏岩夹灰色泥岩组成的盐韵律层存在勘探潜力。

关键词：江汉盆地盐间非砂岩油气藏盐岩层自生自储勘探潜力