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Graft-accelerated virus-induced gene silencing facilitates functional genomics in rose flowers

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Journal of Integrative Plant Biology 2018年第1期60卷 34-44页

作者： Huijun Yan Shaochuan Shi Nan Ma Xiaoqian cao Hao Zhang Xianqin qiu Qigang Wang hongying Jian Ningning Zhou Zhao Zhang Kaixue Tang Flower Research Institute of Yunnan Academy of Agricultural Sciences 650205 Kunming China Beijing Key Laboratory of Development and Quality Control of Ornamental Crops Department of Ornamental Horticulture China AgriculturalUniversity 100193 Beijing China

Rose has emerged as a model ornamental plant for studies of flower development, senescence, and morphology, as well as the metabolism of floral fragrances and ***-induced gene silencing(VIGS) has long been used in functional genomics studies of rose by vacuum infiltration of cuttings or seedlings with an Agrobacterium suspension carrying TRV-derived vectors. However, VIGS in rose flowers remains a challenge because of its low efficiency and long time to establish silencing. Here we present a novel and rapid VIGS method that can be used to analyze gene function in rose,called ‘graft-accelerated VIGS’, where axil ary sprouts are cut from the rose plant and vacuum infiltrated with Agrobacterium. The inoculated scions are then grafted back onto the plants to flower and silencing phenotypes can be observed within 5 weeks, post-infiltration. Using this new method, we successfully silenced expression of the RhDFR, RhA G, and RhNUDXin rose flowers, and affected their color, petal number, as well as fragrance, respectively. This grafting method will facilitate high-throughput functional analysis of genes in rose flowers. Importantly, it may also be applied to other woody species that are not currently amenable to VIGS by conventional leaf or plantlet/seedling infiltration methods.

关键词： TRV Graft-accelerated virus-induced gene silencing facilitates functional genomics in rose flowers Figure DFR

宫颈癌腔内联合组织间插植近距离放疗施源器模板的应用

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中华放射肿瘤学杂志 2024年第2期33卷 137-144页

作者：欧阳翼黄晓丹陈佛平伍海莹叶伟军陈锴李珺芸刘红英麦苗青顾慧宽林焕新曹新平中山大学肿瘤防治中心放疗科、华南肿瘤学国家重点实验室、广东省恶性肿瘤临床医学研究中心广州510060

目的设计适用于局部晚期宫颈癌的腔内联合组织间插植近距离放疗(IC-ISBT)施源器模板,并评估其应用价值。方法收集2019年3月至2020年9月中山大学肿瘤防治中心收治的100例ⅡB-ⅣA期(国际妇产科协会2018分期)宫颈癌患者的外照射前后磁共振... 详细信息

目的设计适用于局部晚期宫颈癌的腔内联合组织间插植近距离放疗(IC-ISBT)施源器模板,并评估其应用价值。方法收集2019年3月至2020年9月中山大学肿瘤防治中心收治的100例ⅡB-ⅣA期(国际妇产科协会2018分期)宫颈癌患者的外照射前后磁共振影像资料,对宫颈原发病灶范围进行回顾性阅片分析对比。基于外照射后肿物残留情况,勾画近距离放疗高危临床靶区(HR-CTV),设计腔内联合组织间插植近距离放疗(IC-ISBT)的施源器模板,并进一步评估施源器模板插植组(37例)与徒手插植组(63例)治疗宫颈癌的剂量和疗效差异。分类变量采用卡方检验或Fisher精确检验,连续变量采用t检验或U检验。结果100例宫颈癌患者外照射后残留肿物边缘按顺时针3、6、9、12点方向与宫颈管中心的距离中位数为16.5、14.0、17.0、13.0 mm。将对应的HR-CTV叠加重建三维图,设计制作蘑菇头圆柱体式IC-ISBT施源器模板:头部长径为35 mm,短径为20 mm,中心适配宫腔管,内圈C1-C12为表盘式排列,两侧拓展B1-B5及A1-A4针道。在剂量方面,模板插植组与徒手插植组的HR-CTV D90%[(635.12±22.65)∶(635.80±25.84)cGy]、膀胱D_(2cm^(3))[(473.79±44.78)∶(463.55±66.43)cGy],直肠D_(2cm^(3))[(396.99±73.54)∶(408.00±73.94)cGy]及乙状结肠D_(2cm^(3))[(293.07±152.72)∶(311.31±135.77)cGy]无明显差异(P>0.05),但前者的HR-CTV D98%较高[(544.78±32.07)∶(536.78±32.04)cGy,P=0.007],直肠D1 cm3及D0.1 cm3较低[(438.62±69.65)∶(453.97±67.89)cGy,P=0.016;(519.46±70.67)∶(543.82±81.24)cGy,P=0.001]。两组的宫颈原发灶完全缓解率(86%∶83%,P>0.05)无差异。结论该IC-ISBT施源器模板设计合理,与徒手插植疗效相当,靶区剂量覆盖符合临床需求,可更好地保护危及器官。其使用操作简便,可重复性强,有望作为常规模板推广应用。

关键词：宫颈肿瘤,局部晚期近距离放射疗法腔内联合组织间插植施源器模板徒手插植剂量分析

肺腺癌患者胸水肿瘤细胞PD-L1/TTF-1表达及应用分析

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标记免疫分析与临床 2024年第3期31卷 405-409,420页

作者：刘菲菲姚健楠谷雨妹赵宏颖曹勍金木兰李雪首都医科大学附属北京朝阳医院病理科北京100020

目的探讨肺腺癌患者胸水肿瘤细胞程序性细胞死亡配体1(PD-L1)/甲状腺转录因子1(TTF-1)、PD-L1免疫组化染色的表达及与组织标本PD-L1表达一致性及其应用研究。方法选取2021年1月至2023年4月间首都医科大学附属北京朝阳医院收治的符合入... 详细信息

目的探讨肺腺癌患者胸水肿瘤细胞程序性细胞死亡配体1(PD-L1)/甲状腺转录因子1(TTF-1)、PD-L1免疫组化染色的表达及与组织标本PD-L1表达一致性及其应用研究。方法选取2021年1月至2023年4月间首都医科大学附属北京朝阳医院收治的符合入组条件的50例肺腺癌患者为研究对象,比较同一病例组织标本中肿瘤细胞的PD-L1表达和胸水细胞蜡块中肿瘤细胞的PD-L1、PD-L1/TTF-1表达情况,评估它们之间表达的一致性。结果50例肺腺癌组织标本PD-L1的表达与患者性别、年龄、标本类型、病灶性质、标本来源和EGFR突变对比差异无统计学意义(P>0.05);胸水细胞蜡块中肿瘤细胞PD-L1/TTF-1免疫组化双染的表达与组织标本PD-L1表达的一致性较好(κ=0.846,P<0.05),明显高于PD-L1免疫组化单染(κ=0.754,P<0.05),与手术或活检切除标本一致性较好(κ=0.90,P<0.05),高于胸腔镜或穿刺小标本(κ=0.82,P<0.05),与原发病灶标本的一致性适中(κ=0.689,P<0.05),与转移病灶标本的一致性较好(κ=0.779,P<0.05)。结论胸水细胞学标本采用PD-L1/TTF-1免疫组化双染与组织标本PD-L1表达一致性较高,细胞蜡块PD-L1/TTF-1双染可在组织不易获取时作为一种有益补充,供临床制定免疫治疗方案参考。

关键词：肺肿瘤腺癌胸水细胞学 PD-L1 TTF-1

SUMOylation-modified Pelota-Hbs1 RNA surveillance complex restricts the infection of potyvirids in plants

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Molecular Plant 2023年第3期16卷 632-642页

作者： Linhao Ge Buwei cao Rui Qiao Hongguang Cui Shaofang Li hongying Shan Pan Gong Mingzhen Zhang Hao Li Aiming Wang Xueping Zhou Fangfang Li State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBejingChina Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests Ministry of Education and College of Plant ProtectionHainan UniversityHaikouHainanChina London Research and Development Centre Agriculture and Agri-Food CanadaLondonONCanada Department of Biology Western UniversityLondonONCanada state Key Laboratory of Rice Biology Institute of BiotechnologyZhejiang UniversityHangzhouZhejiangChina

RNA quality control nonsense-mediated decay is involved in viral restriction in both plants and ***,it is not known whether two other RNA quality control pathways,nonstop decay and no-go decay,are capable of restricting viruses in ***,we show that the evolutionarily conserved Pelota–Hbs1 complex negatively regulates infection of plant viruses in the family Potyviridae(termed potyvirids),the largest group of plant RNA viruses that accounts for more than half of the viral crop damage *** enables the recognition of the functional G1-2A6-7 motif in the P3 cistron,which is conserved in almost all *** allows Pelota to target the virus and act as a viral restriction ***,Pelota interacts with the SUMO E2-conjugating enzyme SCE1 and is SUMOylated in *** Pelota SUMOylation disrupts the ability to recruit Hbs1 and inhibits viral RNA *** findings reveal the functional importance of Pelota SUMOylation during the infection of potyvirids in plants.

关键词： SUMOylation Pelota-Hbs1 G1-2A6-7 motif Potyviruses

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压水堆堆顶结构通风方案设计分析方法研究

广东化工 2024年第9期51卷 101-104页

作者：李浩何培峰李红鹰李海颖余志伟曹立彦核反应堆系统设计技术重点实验室四川成都610000 中国核动力研究设计院四川成都610000

为进一步缩短换料大修时间以提升核电厂经济性,需要对反应堆堆顶结构的通风方案开展优化改进。针对堆顶结构的通风优化改进涉及多专业的复杂性,为此提出了一套面向正向设计的堆顶结构通风方案设计分析方法。该方法通过建立覆盖各专业需... 详细信息

为进一步缩短换料大修时间以提升核电厂经济性,需要对反应堆堆顶结构的通风方案开展优化改进。针对堆顶结构的通风优化改进涉及多专业的复杂性,为此提出了一套面向正向设计的堆顶结构通风方案设计分析方法。该方法通过建立覆盖各专业需求的需求条目,对优化方案进行影响分析,明确后续需开展的仿真计算和分析工作,最终通过对优化方案的流场及温场仿真分析以论证方案的可行性,并明确后续优化方向。本研究为堆顶结构通风冷却方案的优化提供了系统化的设计分析方法,可推广应用于类似的核电设备优化设计中。

关键词：堆顶结构通风冷却设计分析功能需求分析

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钻井稳定剂甲基硅醇钠的合成及性能研究

广东化工 2009年第9期36卷 30-30,29页

作者：尹先清曹红英王正良长江大学化学与环境工程学院湖北荆州434023 中国石油浙江油田公司勘探开发部浙江杭州310023

提出并合成了一种新型有机硅钻井稳定剂CSN。对其抑制性、降粘性和抗盐性进行了室内研究,结果表明,加入CSN的JHG钻井液体系其性能得到提高,有利于保护提高钻井液的性能。

关键词：有机硅钻井稳定剂甲基硅醇钠抑制性抗盐降粘性

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离子通道在阴茎勃起中的作用研究进展

今日药学 2019年第8期29卷 568-571页

作者：伍世颖丁彧许沛珊迪力木拉提·海力帕提许艺飞陈芳军操红缨黄萍广州中医药大学广东广州51006 东莞广州中医药大学中医药数理工程研究院广东东莞523000

阴茎海绵体平滑肌舒张或收缩的调节决定阴茎的勃起和疲软状态。许多研究表明,与血管组织一样,钾和钙离子通道是调节阴茎海绵体平滑肌紧张性的主要离子通道,此外,氯离子通道在阴茎勃起中也起到重要的作用。对海绵体平滑肌细胞和离体阴茎... 详细信息

阴茎海绵体平滑肌舒张或收缩的调节决定阴茎的勃起和疲软状态。许多研究表明,与血管组织一样,钾和钙离子通道是调节阴茎海绵体平滑肌紧张性的主要离子通道,此外,氯离子通道在阴茎勃起中也起到重要的作用。对海绵体平滑肌细胞和离体阴茎海绵体组织平滑肌研究结果显示:参与阴茎勃起的神经递质大多通过对平滑肌细胞离子通道及离子跨膜流动的作用而调节海绵体平滑肌紧张性。

关键词：海绵体平滑肌阴茎勃起平滑肌细胞 Ca^2+通道 Cl^-通道 K^+通道

CMHX008,a PPAR γ partial agonist,enhances insulin sensitivity with minor influences on bone loss

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Genes & Diseases 2018年第3期5卷 290-299页

作者： Yi Hou Xuemei cao Xiangnan Hu Xinyu Li Xiaoqin Shi hongying Wang Chuan Peng Jiayu Li Jibin Li Qifu Li Chaodong Wu Xiaoqiu Xiao Department of Endocrinology The First Affiliated Hospital of Chongqing Medical UniversityChongqing400016China College of Pharmacy Chongqing Medical UniversityChina The Chongqing Key Laboratory of Translational Medicine in Major Metabolic Diseases The First Affiliated Hospital of Chongqing Medical UniversityChongqing400016China Department of Nutrition and Food Hygiene School of Public Health and ManagementChongqing Medical UniversityChongqing400016China Canada-China-New Zealand Joint Laboratory of Maternal and Fetal Medicine Chongqing Medical UniversityChongqing400016China Department of Nutrition and Food Science Texas A&M UniversityCollege StationTX77843USA

Traditional thiazolidinediones(TZDs),such as rosiglitazone,are peroxisome proliferator-activated receptor g(PPARg)potent agonists that can be used to treat type 2 diabetes but carry unwanted effects,including increased risk for *** present work aimed to compare the insulin-sensitizing efficacies and bone-loss side effects of CMHX008,a novel TZDs-like PPARg partial agonist,with those of rosiglitazone.A TR-FRET PPARg competitive binding assay was used to compare the binding affinity between CMHX008 and *** were administered vehicle,CMHX008 or rosiglitazone for 16 *** stem cells(MSCs)were used to examine differences in differentiation into osteoblasts after compounds ***-FRET showed lower affinity to PPARg by CMHX008 compared with *** treated with CMHX008 showed insulin sensitization similar to that of mice treated with rosiglitazone,which was related to the significant inhibition of PPARg Ser273 phosphorylation and improved insulin sensitivity by facilitating the phosphorylation of insulin receptor and Akt in adipose ***-CT and histomorphometric analyses demonstrated that the degree of trabecular bone loss after treatment with CMHX008 was weaker than that observed with rosiglitazone,as evidenced by consistent changes in BV/TV,Tb.N,***,***,and the mineral apposition *** treated with CMHX008 showed higher ALP activity and mRNA levels of bone formation markers than did cells treated with rosiglitazone in the osteoblast differentiation ***,CMHX008 showed insulin-sensitizing effects similar to those of rosiglitazone with a lower risk of bone loss,suggesting that PPARg sparing eliminates the skeletal side effects of TZDs while maintaining their insulin-sensitizing properties.

关键词： Osteoblasts Peroxisome proliferator-activated receptor g Thiazolidinediones TR-FRET Type 2 diabetes mellitus

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大中型泵站供电方案建设探讨

江苏水利 2024年第5期 49-51页

作者：夏鹤鹏陈亚丁邓彬彬曹善宇颜红勤蒋红樱江苏省水利工程科技咨询股份有限公司江苏南京210029 张家港市张水项目管理有限公司江苏苏州215699 张家港市长江防洪工程管理处江苏苏州215625

大中型泵站工程供电方案可靠性对泵站安全稳定运行影响重大,为进一步提高大中型泵站供电方案建设的安全性和可靠性,梳理了37座有代表性的大中型泵站供电情况,针对国家、行业、地方标准规范及相关文件要求,结合省内城市排涝泵站、引调水... 详细信息

大中型泵站工程供电方案可靠性对泵站安全稳定运行影响重大,为进一步提高大中型泵站供电方案建设的安全性和可靠性,梳理了37座有代表性的大中型泵站供电情况,针对国家、行业、地方标准规范及相关文件要求,结合省内城市排涝泵站、引调水泵站供电方案实际运行情况,分析探讨了各供电方案建设的合理性,针对不同类型的泵站工程提出了相应的供电建议方案,为后续新(改)建大中型泵站的供电方案选择提供参考。

关键词：大中型泵站供电方案