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Visual characterization of targeted effect of holo-transferrin-tagged dihydroartemisinin on human breast cancer cells

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Chinese Science Bulletin 2010年第22期55卷 2390-2395页

作者： XIE WeiLing YANG PeiHui ZENG Xin WANG Hui cai HuaiHong cai jiye Department of Chemistry Jinan University Guangzhou 510632 China Key Laboratory of Optoelectronic Information and Sensing Technologies of Guangdong Higher Education Institutes Jinan University Guangzhou 510632 China

Targeted drugs could significantly reduce cytotoxic effect and increase therapeutic activity. Dihydroartemisinin (DHA) has been shown to be effective in killing cancer cells. However, it exhibits non-targeted property. Holo-transferrin (TF) is a suitable drug-carrier to target cancer cells, because cancer cells need iron uptake by the TF-mediated mechanism to maintain their uncontrolled growth. Furthermore, TF receptors (TF-R) are highly expressed on cancer cell surfaces. In this paper, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to evaluate the different killing effect of 4-(12-Dihydroartemisininoxy) Benzoic Acid Hydrozide-transferrin (DBAH-TF) on human breast cancer cells (MCF-7) cells and human normal breast (HNB) cells, and atomic force microscopy (AFM) was used to visually observe the targeted effect of DBAH-TF on MCF-7 cells. MTT results show that DBAH-TF is 172 times more potent than DHA in killing MCF-7 cells, while the cytotoxic effect of DBAH-TF on HNB cells is merely 1/33 to DHA. Also, the killing effect of DBAH-TF on MCF-7 cells is 286 times that on HNB cells, showing targeted effect. Moreover, there are distinct differences in ultrastructures of cellular surfaces after DBAH-TF and DHA treatment. Through AFM imaging, many characteristic holes were observed on the cancer cell surface after being effected by DBAH-TF, which differ from the holes with irregular shapes affected by DHA. These results visually show that the DBAH-TF targeted drug has more potent killing effect on MCF-7 cells compared with DHA.

关键词：人乳腺癌细胞双氢青蒿素视觉特性全息转铁蛋白受体原子力显微镜转移因子细胞毒作用

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PROBING MOLECULAR INTERACTION BETWEEN TRANSFERRIN AND ANTI-TRANSFERRIN BY ATOMIC FORCE MICROSCOPE

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Chinese Science Bulletin 2006年第4期51卷 405-408页

作者： ZHENG Zhiwen YANG Peihui ZENG Gucheng cai jiye Key Laboratory of Specially Functional Materials and AdvancedManufacturing Technology Ministry of Education South China University of Technology Guangzhou 510640 China Department of Chemistry Jinan University Guangzhou 510632 China

The interaction between transferrin (Tf) and its antibody was investigated by atomic force microscope. Tf-antibody was immobilized on the Au-coated glass slide, and the specific combination between antibody and antigen was also character-ized by AFM. The results showed that holo-transferrin was jogged with anti-transferrin, and binded anti-tran- sferrin more tightly than apo-transferrin. The force- distance curves revealed that the affinity of anti-trans- ferrin and holo-transferrin was much stronger than that of apo-transferrin.

关键词：转铁球蛋白抗转铁球蛋白分子间相互作用原子力显微镜生物大分子

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Cinobufacini-induced HeLa cell apoptosis enhanced by curcumin

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Chinese Science Bulletin 2013年第21期58卷 2584-2593页

作者： LIU Li JIN Hua OU JinLai JIANG JinHuan PI Jiang KE ChangHong YANG Fen QIAO DongJuan cai HuaiHong cai jiye Department of Chemistry and Institute for Nano-Chemistry Jinan University Department of Pharmaceutics College of Pharmacy Jinan University

When used in combination with certain chemotherapies, curcumin has been shown to increase apoptosis in several cancer cell lines. Here, we report the combined effects of curcumin and cinobufacini on human cervical carcinoma cells. The aim of this study was to examine whether curcumin could enhance apoptosis induced by cinobufacini. 3-(4,5-Dimethylthiazol-2-y1)-2,5- diphenytetrazolium bromide (MTT) assays revealed that the growth and proliferation of HeLa cells could be inhibited by 75% after a combined treatment of 25 μg/mL cinobufacini and 8 μg/mL curcumin. The combined treatment is 3 times more effective than treatment with 25 μg/mL cinobufacini alone. Annexin V-FITC/PI staining, morphological changes and immunofluorescence verified a significant enhancement in cinobufacini-induced apoptosis when cells were also exposed to curcumin. The data showed that the proportion of early apoptotic cells significantly increased from 15.43% in cells treated only with 25 μg/mL cinobufacini to 49.2% in cells treated with 25 μg/mL cinobufacini and 8 μg/mL curcumin. Moreover, compared with treatment of only 25 μg/mL cinobufacini, ROS production increased 1.7-fold, the intracellular free Ca 2+ concentration increased 1.5-fold, and the mitochon- drial membrane potential decreased by 20% in the combined treatment. Simultaneously, the atomic force microscopy (AFM) re- sults suggest that cells treated with a combination of cinobufacini and curcumin varied significantly in shape and ultrastructure. Collapsed cells with leaking cytoplasm, blebbing pores and emerging apoptotic bodies were prevalent. The nanoparticle size increased from 70 nm when the cells were treated with 25 μg/mL cinobufacini to 190 nm when the cells were treated with 25 μg/mL cinobufacini and 8 μg/mL curcumin. The size increase resulted in the cell membrane becoming considerably rough. These results can improve our understanding of combination treatments. Specifically, the combination of cinobufacini and curcumin may

关键词： HeLa细胞细胞凋亡姜黄素诱导原子力显微镜超微结构变化蟾联合治疗

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基于Fluent软件的直喷柴油机螺旋进气道设计

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农业机械学报 2006年第6期37卷 1-4页

作者：蔡忆昔蔡继业江苏大学汽车与交通工程学院

利用Catia进行三维造型、用Fluent仿真模拟作为辅助手段来设计进气道。在分析总结螺旋进气道结构特点的基础上,针对小型柴油机的特点,设计出了引导管较短、较陡的螺旋进气道,建立了该进气管的3-D模型,并借助Fluent软件对该气道内的气流... 详细信息

利用Catia进行三维造型、用Fluent仿真模拟作为辅助手段来设计进气道。在分析总结螺旋进气道结构特点的基础上,针对小型柴油机的特点,设计出了引导管较短、较陡的螺旋进气道,建立了该进气管的3-D模型,并借助Fluent软件对该气道内的气流进行了仿真模拟,得到了对进气道结构改进有价值的结论。

关键词：柴油机螺旋进气道流场模拟

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Detection of Lipopolysaccharide Induced Inflammatory Responses and Anti-inflammatory Effects of Quercetin in RAW264.7 Macrophages Using Atomic Force Microscope

Detection of Lipopolysaccharide Induced Inflammatory Respons...

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第十四届全国中药和天然药物学术研讨会

作者： Jiang Pi jiye cai SMacau University of Science and Technology

＜正＞In recent years,lipopolysaccharide（LPS）activated RAW264.7 cells are widely used as an in vitro inflammatory model for the screen of effective anti-inflammation drugs and the investigation of their exact anti-inflammation *** up to now,there are few data about the effect of LPS on the morphology,especially on the membrane ultrastructure and

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扫描近场光学显微镜及其在单分子探测中的应用

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化学进展 2008年第6期20卷 984-988页

作者：胡明铅蔡继业暨南大学生命科学技术学院化学系广州510632

扫描近场光学显微镜在光学显微镜中具有独特的性能,其突破衍射光限制,具有单分子探测灵敏度,且在研究时不损伤生物样品。文中简要介绍了扫描近场光学显微镜的原理,详述近年来扫描近场光学显微镜在单分子探测中的应用,介绍了扫描近场光... 详细信息

扫描近场光学显微镜在光学显微镜中具有独特的性能,其突破衍射光限制,具有单分子探测灵敏度,且在研究时不损伤生物样品。文中简要介绍了扫描近场光学显微镜的原理,详述近年来扫描近场光学显微镜在单分子探测中的应用,介绍了扫描近场光学显微镜结合量子点对单分子探测的进展,并对单分子探测的前景做了展望。

关键词：扫描近场光学显微镜单分子探测量子点

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An Indirect Immunoassay for Detecting Antigen Based on Fluorescence Resonance Energy Transfer

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American Journal of Analytical Chemistry 2011年第4期2卷 484-490页

作者： Peihui Yang Shuguang Yao Wei Wei jiye cai 不详

An indirect immunoassay for detecting antigen was developed. It was based on fluorescence resonance energy transfer (FRET) and quenching of gold nanoparticles. Bovine serum albumin (BSA) was chosen as model antigen. Fluorescein isothiocyanate (FITC) was attached to anti-BSA antibody (anti-BSA–FITC) as FRET donor, while BSA was conjugated to gold nanoparticles (GNPs–BSA) as FRET acceptor. The formation of anti-BSA–BSA immunocomplex resulted in the FRET between anti-BSA–FITC and GNPs–BSA. Thus, the fluorescence of FRET donor was quenched, and the decreasing fluorescence intensity responded linearly to the concentration of acceptor within the linear range. The concentration of BSA we obtained according to the stoichiometric ratio between BSA and GNPs. Following this approach, we were able to specifically detect BSA. The detection limit for BSA was 0.5 nM and the linear range of the assay was 2.9 - 43.5 nM. It had been successfully applied to specific detection of BSA in serum samples.

关键词： Anti-BSA Antibody BSA Gold Nanoparticles FRET Indirect Immunoassay

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磷脂自组装膜的原子力显微镜研究进展

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生物医学工程学杂志 2008年第2期25卷 472-475页

作者：邓穗平蔡继业暨南大学生物医学工程研究所广州510632

利用磷脂自组装膜在分子水平上研究生物分子的结构和性能,在生物学和仿生学方面有重要研究意义。以原子力显微镜(AFM)为重要的研究工具,综述了磷脂自组装膜的表面形貌、生长动力学的研究进展以及磷脂自组装膜在生物大分子如蛋白质、DNA... 详细信息

利用磷脂自组装膜在分子水平上研究生物分子的结构和性能,在生物学和仿生学方面有重要研究意义。以原子力显微镜(AFM)为重要的研究工具,综述了磷脂自组装膜的表面形貌、生长动力学的研究进展以及磷脂自组装膜在生物大分子如蛋白质、DNA和酶等的高分辨率AFM成像中的应用。

关键词：自组装膜磷脂原子力显微镜高分辨率蛋白质 DNA 酶

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A novel mechanism of transient cellular self-protection against chemotherapeutical drugs via stimulating Intercellular ABCB1 transfer

A novel mechanism of transient cellular self-protection agai...

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广东省生物物理学会2013年学术研讨会

作者： Dongjuan Qiao Xiaokun Wang jiye cai Liwu Fu Department of Chemistry College of Life Science and TechnologyJinan University Sun Yat-sen University Cancer Center

ABCB1(P-gp/MDRl/ABCBl),one of the most important mediator of multidrug resistance (MDR),has been observed as a result of the selective pressure from chemotherapy and is associated with poor clinical *** work has found that individual cells communicate with each other by exchanging the contents of their membrane protein ABCB1. However,the study to investigate whether the transfers of ABCB1 are stimulated by the presence of chemotherapeutical drugs has not yet been *** this study,we provided strong evidence for the existence of the functional ABCB1 transfers from drug-resistant cells to sensitive ***,it was found for the first time that a short-time stimulus of chemotherapeutical drugs could concentration-dependently boost the intercellular transfer of ABCB1,but no inducible ***,chemotherapeutical drugs doxorubicine(Dox) significantly increased micropaticles(Mps) secreted by drug-resistant cells(KBv200),which were reported to serve as vectors in the intercellular transfer of ABCB1,and effectively stimulated this Mps-mediated intercellular transfer of *** findings reveal a novel mechanism of transient cellular self-protection against exposure to chemotherapeutical drugs,that is intercellular ABCB1 transfer resulting in the increase of multicellular overall *** it has important implications for interpreting the link between chemotherapy and acquired MDR.

关键词： ABCB1 transient transfer overall resistance chemotherapeutical drugs

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纳米银的制备及应用研究进展

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材料导报 2009年第S1期23卷 15-18页

作者：徐进之蔡继业暨南大学化学系

纳米银具有独特的热、光、电、磁、催化和敏感等特性,具有广阔的应用前景,是金属纳米材料研究的热点。阐述了制备纳米银的方法,包括化学还原法、光化学还原法、模板法、溶胶-凝胶法、微乳液法、激光烧蚀法等,列举了纳米银在化学反应、... 详细信息

纳米银具有独特的热、光、电、磁、催化和敏感等特性,具有广阔的应用前景,是金属纳米材料研究的热点。阐述了制备纳米银的方法,包括化学还原法、光化学还原法、模板法、溶胶-凝胶法、微乳液法、激光烧蚀法等,列举了纳米银在化学反应、光学领域、抗菌领域和作为抗静电材料的主要应用,简述了纳米银制备过程中存在的不足,展望了纳米银合成研究的发展趋势。

关键词：纳米银制备方法应用

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