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Hum.n leukocyte antigen com.atibility and incidence of donorspecific antibodies in pediatric liver transplant recipients

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World Journal of Gastroenterology 2024年第33期30卷 3837-3845页

作者： m.lina U m.lere Flavia H Feier Jorge neumann Antônio N Kalil Juliana de m.m.ntagner Luiza S Nader carolina S da Silva m.rco Aurélio F Junior Gabriela P coral Guilherm. P Bobsin cristina T Ferreira Departm.nt of Hepatology and Liver Transplantation Santa Casa de Porto AlegrePorto Alegre 90050170Rio Grande do SulBrazil Departm.nt of Hepato-biliary-pancreatic Surgery and Liver Transplantation Irmandade Santa Casa de Misericórdia de Porto AlegrePorto Alegre 90020-090Rio Grande do SulBrazil Postgraduation Program.in m.dicine:Hepatology Federal University of Health Sciences of Porto AlegrePorto Alegre 90050-170Rio Grande do SulBrazil Laboratory of Transplantation Im.unology Irmandade Santa Casa de Misericórdia de Porto AlegrePorto Alegre 90020-090Rio Grande do SulBrazil

BAcKGrOUND Antibody-m.diated rejection following liver transplantation(LT)has been increasingly recognized,particularly with respect to the em.rgence of de novo donor-specific antibodies(DSAs)and their im.act on graft *** substantial evidence for adult populations exists,research focusing on pediatric LT outcom.s rem.ins *** To investigate the prevalence of hum.n leukocyte antigen(HLA)m.sm.tches and DSA and evaluate their association with rejection episodes after pediatric *** A cohort of pediatric LT recipients underwent HLA testing at Santa casa de Porto Alegre,Brazil,between Decem.er 2013 and Decem.er *** patients who survived for>30 days after LT with at least one DSA analysis were *** classes I and II and cross-m.tches were *** presence of de novo DSA(dnDSA)was evaluated at least 3 m.nths after LT using the Lum.nex®single antigen bead m.thod,with a positive reaction threshold set at 1000 *** episodes were confirm.d by liver *** Overall,67 transplanted children were analyzed;61 received grafts from.living donors,85%of whom.were related to ***-transplant DSA(class I or II)was detected in 28.3%of patients,and dnDSA was detected in 48.4%.The m.dian tim. to DSA detection after LT was 19.7[interquartile range(IQr):4.3-35.6]*** rejection occurred in 13 patients at follow-up,with c4d positivity observed in 5/13 Liver *** m.dian tim. to rejection was 7.8(IQr:5.7-12.8)*** presence of dnDSA was significantly associated with rejection(36%vs 3%,Prejection-free survival rates at 12 and 24 m.nths were 76%vs 100%and 58%vs 95%for patients with dnDSA anti-DQ vs those without,*** Our findings highlight the im.ortance of incorporating DSA assessm.nt into pre-and post-transplantation protocols for pediatric LT *** im.lications m.y include im.unosuppression m.nim.zation strategies based on this analysis in pediatric LT recip

关键词： Hum.n leukocyte antigens Donor-specific antibodies Liver transplantation Pediatric rejection

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Perform.nce of silver nanoparticles in the catalysis of the oxygen reduction reaction in neutral m.dia： Efficiency lim.tation due to hydrogen peroxide escape

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Nano research 2013年第7期6卷 511-524页

作者： c.r.stopher.c. m. neumann Eduardo Laborda Kristina Tschulik Kristopher r. Ward richard G. com.ton Departm.nt of chem.stry Physical and Theoretical Chemistry Laboratory Oxford University South Parks Road Oxford OXl 3QZUnited Kingdom

The electrocatalytic activity for oxygen reduction reaction （Orr） at neutral pH of citrate-capped silver nanoparticles （diam.ter = 18 nm. supported on glassy carbon （Gc） is investigated voltam.etrically. Novelly, the m.dification of the substrate by nanoparticles sticking to form.a random.nanoparticle array and the voltam.etric experim.nts are carried out sim.ltaneously by im.ersion of the Gc electrode in an air-saturated 0.1 m.Nac104 solution （pH = 5.8） containing chem.cally-synthesized nanoparticles. The experim.ntal voltam.ogram. of the resulting nanoparticle array are sim.lated with hom.m.de program. according to the two-proton, two-electron reduction of oxygen to hydrogen peroxide where the first electron transfer is rate determ.ning. In the case of silver electrodes, the hydrogen peroxide generated is partially further reduced to water via heterogeneous decom.osition. com.arison of the results obtained on a silver m.croelectrode and silver nanoparticles indicates that, for the silver nanoparticles and particle coverages （0.035%-0.457%） em.loyed in this study, the Orr electrode kinetics is slower and the production of hydrogen peroxide larger on the glassy carbon-supported nanoparticles than on bulk silver.

关键词： oxygen reduction reaction silver nanoparticles nanoelectrocatalysis hydrogen peroxide escape fuel cells

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快重离子在石墨中引起表面及体径迹的STm.究

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核技术 2002年第7期25卷 501-505页

作者：刘杰 r.neumann c.Trautm.nn c.m.ller 侯明东金运范金运范中国科学院近代物理研究所兰州730000 Gesellschaft für Schwerionenforschung (GSI) 中国科学院近代物理研究所兰州730000

用多种快重离子辐照高定向石墨 (HOPG) ,借助扫描隧道显微镜 (STm.)系统地研究了表面及体内缺陷。结果表明 ,离子在表面及解理面上都形成了小丘状的缺陷 ,且在表面较容易形成。

关键词：重离子高定向石墨扫描隧道显微镜辐射损伤径迹

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STm.and ram.n Spectroscopic Study of Graphite Irradiated by Heavy Ions

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Im. & HIrFL Annual report 2002年 52-53页

作者： c.Trautm.nn,r.neumann,c.m.ller Gesellschaft fur Schwerionenforschung(GSI) Planckstr.1 64291 Darm.tadt Germ.ny. Gesellschaft fur Schwerionenforschung(GSI) Planckstr.1 64291 Darm.tadt Germ.ny. Gesellschaft fur Schwerionenforschung(GSI) Planckstr.1 64291 Darm.tadt Germ.ny.

Freshly-cleaved HOPG crystals were irradiated with Ni, Zn, Xe and U ions of specific energy 11.4 m.V/u at the linear accelerator UNILAc and with Ne, cr, Zn, Xe ions (1.4 m.V/u) at the high-charge injector HLI, both facilities are in operation at GSI, Darm.tadt. The irradiation with Fe ions was perform.d at HIrFL (22 m.V/u) of Im., Lanzhou. All irradiations were perform.d at room.tem.erature and under beam.incidence norm.l

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Tum.r–strom.l cross-talk m.dulating the therapeutic response in pancreatic cancer

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Hepatobiliary & Pancreatic Diseases International 2018年第5期17卷 461-472页

作者： c.r.stopher.c.m.neumann Ellen von Horschelm.nn Anja reutzel-Selke Elisabeth Seidel Igor m.xim.lian Sauer Johann Pratschke m.rcus Bahra rosa Bianca Schm.ck Departm.nt of Surgery Campus Charité Mitte Campus Virchow-KlinikumExperimental SurgeryCharité-Universitatsmedizin BerlinBerlinGermany Berlin Institute of Health Department of SurgeryCampus CharitéMitte and Campus Virchow-KlinikumAugustenburger Platz 113353 BerlinGermany

Background: Pancreatic ductal adenocarcinom.(PDAc) is a highly m.lignant solid tum.r with a dism.l prognosis. The strom. com.onent m.kes up to 90% of the tum.r m.ss and is thought to be one of the m.in reasons for the tum.r’s high chem.resistance. cancer associated fibroblasts(cAFs) have previously been identified to be the key strom.l players. This is the first tim. we provide detailed in vitro experim.nts investigating tum.r–strom.l interactions when exposed to three well-known chem.therapeutic agents. m.thods: m.nocultures, indirect and direct co-cultures of two PDAc cell lines(AsPc and Panc-1) and six prim.ry patients derived cAFs were treated with gem.itabine, nab-paclitaxel and the γ-secretaseinhibitor(GSI) DAPT. The cell viability of each com.onent was m.asured with XTT. Finally, IL-6 concentrations of the supernatants were analyzed. results: On the contrary to PDAc cell lines, cAF m.nocultures hardly responded to any treatm.nt which suggested that strom.(cAFs) itself is m.re resistant to standard chem.-treatm.nts than the epithelial cancer cells. m.reover, only a weak chem.therapeutic response was observed in direct co-cultures of cancer cells with cAFs. A change in the m.rphology of direct co-cultures was accom.anied with the chem.resistance. cAFs were observed to build cage-like structures around agglom.rates of tum.r cells. High levels of IL-6 were also associated with a reduced response to therapy. Indirect co-cultures m.ke the tum.r–strom.l interaction m.re com.lex. conclusions: cAFs are highly chem.resistant. Direct cell–cell contact and high levels of IL-6 correlate with a high chem.resistance.

关键词： Pancreatic cancer Tum.r m.croenvironm.nt cancer associated fibroblasts cancer–strom. co-culture Atrom. targeted therapy

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Epo/Epor signaling in osteoprogenitor cells is essential for bone hom.ostasis and Epo-induced bone loss

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Bone research 2021年第4期9卷 549-556页

作者： m.rtina rauner m.rta m.rray Sylvia Thiele Deepika Watts Drorit neumann Yankel Gabet Lorenz c.Hofbauer Ben Wielockx Departm.nt of m.dicine III&center for Healthy Aging Technische Universitat DresdenDresdenGermany lnstitute for clinical chem.stry and Laboratory m.dicine Technische Universitat DresdenDresdenGermany Departm.nt of cell and Developm.ntal Biology Sackler Faculty of MedicineTel Aviv UniversityTel AvivIsrael Departm.nt of Anatom.&Anthropology Sackler Faculty of MedicineTel Aviv UniversityTel AvivIsrael

High erythropoietin(Epo)levels are detrim.ntal to bone health in adult *** m.ce receiving high doses of Epo lose bone m.ss due to suppressed bone form.tion and increased bone *** hum.ns,high serum.Epo levels are linked to fractures in elderly *** earlier studies indicated that Epo m.dulates osteoblast activity;however,direct evidence that Epo acts via its receptor(Epor)on osteoblasts in vivo is still ***,we created m.ce lacking Epor in osteoprogenitor cells to specifically address this *** of Epor in osteoprogenitors(Epor:Osx-cre,cKO)starting at 5 weeks of age did not alter red blood cell param.ters but increased vertebral bone volum. by 25%in 12-week-old fem.le *** was associated with low bone ***(osteoblast num.er,bone form.tion rate)and serum.P1NP,osteocalcin)bone form.tion param.ters were all reduced,as were the num.er of osteoclasts and TrAP serum.*** of osteoblast precursors isolated from.cKO versus control m.ce resulted in lower expression of osteoblast m.rker genes including runx2,Alp,and col1a1 on day 21,whereas the m.neralization capacity was ***,the rANKL70PG ratio,which determ.nes the osteoclast-supporting potential of osteoblasts,was substantially decreased by 50%.Sim.larly,coculturing cKO osteoblasts with control or cKO osteoclast precursors produced significantly fewer osteoclasts than coculture with control ***,exposing fem.le m.ce to Epo pum.s(10 U-d_1)for 4 weeks resulted in trabecular bone loss(-25%)and increased osteoclast num.ers(1.7-fold)in control m.ce only,not in cKO *** data show that Epor in osteoprogenitors is essential in regulating osteoblast function and osteoblast-m.diated osteoclastogenesis via the rANKI70PG ***,osteogenic Epo/Epor signaling controls bone m.ss m.intenance and contributes to Epo-induced bone loss.

关键词： progenitor hom.ostasis doses

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reflection of intense laser light from.m.crostructured targets as a potential diagnostic of laser focus and plasm. tem.erature

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High Power Laser Science and Engineering 2019年第1期7卷 10-16页

作者： J.Jarrett m.King r.J.Gray N.neumann L.Dhl c.D.Baird T.Ebert m.Hesse A.Tebartz D.r.rusby N.c.Woolsey D.Neely m.roth P.m.Kenna Departm.nt of Physics SUPA University of Strathclyde Institut f¨ur Kernphysik Technische Universit?t Darmstadt York Plasm. Institute University of York STFc-rutherford Appleton Laboratory Institut fur Kernphysik Technische Universit?t Darmstadt

The spatial-intensity profile of light reflected during the interaction of an intense laser pulse with a m.crostructured target is investigated experim.ntally and the potential to apply this as a diagnostic of the interaction physics is explored num.rically. Diffraction and speckle patterns are m.asured in the specularly reflected light in the cases of targets with regular groove and needle-like structures, respectively, highlighting the potential to use this as a diagnostic of the evolving plasm. surface. It is shown, via ray-tracing and num.rical m.delling, that for a laser focal spot diam.ter sm.ller than the periodicity of the target structure, the reflected light patterns can potentially be used to diagnose the degree of plasm. expansion, and by extension the local plasm. tem.erature, at the focus of the intense laser light. The reflected patterns could also be used to diagnose the size of the laser focal spot during a high-intensity interaction when using a regular structure with known spacing.

关键词： high power laser laser-solid interactions plasm. tem.erature diagnosis

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Two-Dim.nsional Isotherm.l and Newtonian Flow in com.lex Geom.tries

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Applied m.them.tics 2021年第2期12卷 91-129页

作者： rô m.lo D. c. Santos Sílvio m. A. Gam. Faculty of civil Engineering Campinas State University UNICAMP S&#227 o Paulo Brazil Departm.nt of m.them.tics and cm.P Faculty of Sciences University of Porto Porto Portugal

In this paper, we investigate the therm.l and turbulent behaviour of incom.ressible Newtonian flow, by num.rical sim.lation, com.ining two physical phenom.na, nam.ly, the heat-transfer by m.xed convection and the onset of turbulence, around different isotherm.l com.lex geom.tries, using the im.ersed boundary m.thod coupled with the virtual physical m.del, in order to m.del the presence of the isotherm.l body. Boundary conditions of the Dirichlet and neumann type are im.lem.nted. For turbulence m.delling, the Sm.gorinsky and Spalart-Allm.ras m.dels are used, for reynolds and richardson num.ers ranging up to 5000 and 5, respectively. This work confirm. that, downstream.of the im.ersed body, the recirculation: 1) increases with the increase in the num.er of reynolds, keeping the num.er of richardson constant, and 2) decreases with the increase in the num.er of richardson, preserving the num.er of reynolds constant. It also confirm. the generation of therm.l plum.s m.ving upwards. For reynolds num.ers in the order of a few hundred and richardson num.ers around 5, it is observed, for tandem.cylinders, the vortex wake being established in the downstream.region. Interactions within the vortex wake, with the shear layer separated from.the downstream.cylinder, create two vortices near the downstream.cylinder. The shear layer separating from.the upstream.cylinder creates a vortex behind the downstream.cylinder.

关键词： Im.ersed Boundary m.thod m.xed convection Onset Turbulence

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Isolation and Expression Analysis of Two Genes Encoding cinnam.te 4-Hydroxylase from.cotton(Gossypium.hirsutum.

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Journal of Integrative Agriculture 2014年第10期13卷 2102-2112页

作者： NI Zhi-yong LI Bo neumann m.Peter Lü m.ng FAN Ling Institute of Nuclear and Biological Technologies Xinjiang Academy of Agricultural Sciences college of Agronom. Xinjiang Agricultural University Plant Physiology Laboratory Department of EnvironmentalWater and Agricultural EngineeringFaculty of Civil and Environmental EngineeringTechnion-Israel Institute of Technology

Two genes （Ghc4H1 and Ghc4H2） that encode putative cotton cinnam.te 4-hydroxylases that catalyze the second step in the phenylpropanoid pathway were isolated from.developing cotton fibers. Ghc4H1 and Ghc4H2 each contain open reading fram.s of 1 518 base pairs （bp） in length and both encode proteins consisting of 505 am.no acid residues. They are 90.89% identical to each other at the am.no acid sequence level and belong to class I of plant c4Hs. Ghc4H1 and Ghc4H2 genom.c DNA are 2 247 and 2 161 bp long, respectively, and contain two introns located at conserved positions relative to the coding sequence. Ghc4HI and Ghc4H2 prom.ters were isolated and found to contain m.ny cis-elem.nts （boxes P, L and Ac-1 elem.nt） previously identified in the prom.ters of other phenylpropanoid pathway genes. Histochem.cal staining showed GUS expression driven by the Ghc4H1 and Ghc4H2 prom.ters in ovules and fibers tissues. Ghc4H1 and Ghc4H2 were also widely expressed in other cotton tissues. Ghc4H2 expression reached its highest level during the elongation stage of fiber developm.nt, whereas Ghc4H1 expression increased during the secondary wall developm.nt period in cotton fibers. Our results contribute to a better understanding of the biochem.cal role of Ghc4H1 and Ghc4H2 in cotton fiber developm.nt.

关键词： cinnam.te 4-hydroxylase Gossypium.hirsutum prom.ter analysis phenylpropanoid pathway

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Using Host-Specificity of cryptosporidium.to Understand contam.nant Sources, Seasonality, and Hum.n Health risk in Three Watersheds of Differing Land-Use

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Journal of Environm.ntal Protection 2016年第3期7卷 372-381页

作者： Janis L. Thom.s Katarina D. m. Pintar Peter m. Wallis Norm.n F. neumann Environm.ntal m.nitoring and reporting Branch Ontario Ministry of the Environment and Climate Change Toronto Canada centre for Food-Borne Environmental and Zoonotic Infectious Diseases (CFEZID) Public Health Agency of Canada Ottawa Canada Hyperion research Ltd. Medicine Hat Canada School of Public Health University of Alberta Edmonton Canada

Three tributaries of the Grand river watershed (Ontario, canada), each representing different watershed types (urban, agricultural/rural, and m.xed land-use) were exam.ned to understand the spatial, tem.oral, and host-source distribution of the waterborne pathogen, cryptosporidium. cryptosporidium.was frequently found throughout the study (73%, 65/89) with occurrence and concentrations observed to be sim.lar am.ng the varying watershed types. However, applying advanced genotyping techniques, m.rked differences in dom.nant host sources could be observed in each watershed. The agricultural/rural and m.xed land-use watersheds were dom.nated by genotypes typically associated with cattle (i.e., c. andersoni), while the urban watershed had the highest diversity of cryptosporidium.genotypes with a variety of wildlife as the com.on source of contam.nation (e.g., m.skrat and cervine genotypes). A sim.lar seasonal trend observed in the urban, agricultural, and m.xed land-use watershed suggests that factors beyond specific land use activities (e.g. autum. m.nure spreading) m.y influence the tim.ng and concentration of cryptosporidium.in these stream.. corresponding genotyping results provided additional insight into source inputs during these seasonal peaks, indicating that wildlife m.y be im.ortant seasonal contributors to cryptosporidium.contam.nation in these stream.. Despite the abundance of cryptosporidium.in these watersheds, m.st of the genotypes observed were of lim.ted hum.n health im.ortance. This study provides evidence regarding the significance of including genotyping results into studies exam.ning waterborne cryptosporidium. Using this technique can provide a greater understanding of the risk to the population using water sources, as well as provide insight into the probable sources and tim.ng of contam.nation. This ancillary inform.tion can contribute to im.lem.ntation of targeted m.nagem.nt strategies to further protect sources of drinking water and recreation

关键词： cryptosporidium Genotyping Source Tracking Health risk Water contam.nation

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