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Dried blood spot sampling as an alternative for the improvement of hepatitis B and c diagnosis in key populations

World Journal of Hepatology 2021年第4期13卷 504-514页

作者： Geane Lopes Flores Jakeline ribeiro Barbosa Helena Medina cruz Juliana custódio Miguel Denise Vigo Potsch JoséHenrique Pilotto Danielle Malta Lima JeováKeny Baima colares c.r.os Eduardo Brandão-Mello Marcia Maria Amendola pires Jurema corrêa da Mota Francisco I Bastos Lia Laura Lewis-Ximenez Livia Melo Villar Viral Hepatitis Laboratory FIOCRUZRio de Janeiro 21040360Brazil Postgraduate Program in Pathology Federal University of CearáFortaleza 60020181CearaBrazil clementino Fraga Filho Hospital Federal University of Rio de JaneiroRio de Janeiro 21040360Brazil Nova Iguaçu General Hospital Nova Iguaçu 26041061Brazil Molecular Immunology Laboratory Oswaldo Cruz InstituteRio de Janeiro 21040360Brazil Postgraduate Program in Medical Sciences University of FortalezaFortaleza 60811905CearáBrazil Escola de Medicina e cirurgia Gaffrée&Guinle University HospitalFederal University of Rio de Janeiro StateRio de Janeiro 20270901Brazil Institute of communication and Information on Science and Technology in Health FIOCRUZRio de Janeiro 21040360Brazil

BAcKGrOUND To achieve the elimination of hepatitis B and c,there is an urgent need to develop alternative strategies to increase the access of diagnosis,particularly among key populations such as people living with human immunodeficiency virus(HIV),individuals with coagulopathies and chronic kidney disease(cKD)*** To evaluate the use of dried blood spot(DBS)in the detection of hepatitis B virus(HBV)and hepatitis c virus(HcV)*** A total of 430 individuals comprised of people living with HIV,coagulopathies and cKD provided paired serum and DBS ***,anti-HBc and anti-HcV were tested in those samples using a commercial *** and selected behavioral variables were evaluated to assess possible association with HBV and HcV *** Using DBS,HBsAg prevalence varied from 3.9%to 22.1%,anti-HBc rates varied from 25.5%to 45.6%and anti-HcV positivity ranged from 15.9%to 41.2%in key *** of HBV and HcV tests using DBS varied from 88.9%to 100%.The HBsAg assay demonstrated the best performance in cKD and coagulopathy individuals and the anti-HcV test had a sensitivity and specificity of 100%in people living with *** of HBV and HcV detection in DBS varied from 90.2%to 100%.In the cKD group,HBsAg positivity was associated with infrequent use of condoms,and anti-HBc positivity was associated with sharing nail cutters/razors/***-HcV reactivity was positively associated with a history of transplantation and length of time using hemodialysis in both *** people living with HIV,only the male gender was associated with anti-HBc positivity in serum and *** DBS with electrochemiluminescence are useful tools for the diagnosis and prevalence studies of hepatitis B and c among key populations and may increase the opportunity to foster prevention and treatment.

关键词： Dried blood spot Electrochemiluminescence Hepatitis B Hepatitis c Key populations Diagnosis

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Evaluation of biodegradable electric conductive tube-guides and mesenchymal stem cells

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World Journal of Stem cells 2015年第6期7卷 956-975页

作者： Jorge ribeiro Tiago Pereira Ana rita caseiro Paulo Armada-da-Silva Isabel pires Justina Prada Irina Amorim Sandra Amado Miguel Franca carolina Goncalves Maria Ascensao Lopes Jose Domingos Santos Dina Morais Silva Stefano Geuna Ana Lúcia Luís Ana colette Maurício Departmento de clínicas Veterinárias and+UPVet Instituto de Ciencias Biomedicas de Abel Salazar(ICBAS)and Centro de Estudos de Ciencia Animal(CECA)Instituto de CienciasTecnologias e Agroambiente da Universidade do Porto(ICETA)Rua de Jorge Viterbo Ferreira4050-313 PortoPortugal centro Interdisciplinar de Estudo de Performance Humana(cIPEr) Faculdade de Motricidade Humana(FMH)Universidade de Lisboa(UL)Estrada da Costa1499-002 Cruz QuebradaDafundoPortugal cEcAV and Departamento de ciencias Veterinárias Universidade de Trás-os-Montes e Alto Douro(UTAD)5001-801 Vila RealPortugal Departmento de Patologia e de Imunologia Molecular Instituto de Ciencias Biomedicas de Abel SalazarRua de Jorge Viterbo Ferreira4050-313 PortoPortugal Instituto Português de Patologia e Imunologia Molecular da Universidade do Porto(IPATIMUP) Rua Dr.Roberto Frias s/n4200-465 PortoPortugal UIS-IPL:Unidade de Investigacao em Saúde da Escola Superior de Saúde de Leiria Instituto Politécnico de Leiria2411-901 LeiriaPortugal cEMUc Departamento de Engenharia Metalúrgica e MateriaisFaculdade de Engenharia da Universidade do Porto(FEUP)Rua Dr.Roberto Frias4200-465 PortoPortugal Biosckin Molecular and Cell Therapies S.A.Tec MaiaRua Engenheiro Frederico Ulrich 26504470-605 MaiaPortugal Neuroscience Institute of the cavalieri Ottolenghi Foundation and Department of clinical and Biological Sciences University of Turin

AIM: To study the therapeutic effect of three tubeguides with electrical conductivity associated to mesenchymal stem cells(MScs) on neuro-muscular regeneration after ***: rats with 10-mm gap nerve injury were tested using polyvinyl alcohol(PVA), PVA-carbon nanotubes(cNTs) and MScs, and PVA-polypyrrole(PPy). The regenerated nerves and tibialis anterior muscles were processed for stereological studies after 20 wk. The functional recovery was assessed serially for gait biomechanical analysis, by extensor postural thrust, sciatic functional index and static sciatic functionalindex(SSI), and by withdrawal reflex latency(WrL). In vitro studies included cytocompatibility, flow cytometry, reverse transcriptase polymerase chain reaction and karyotype analysis of the MScs. Histopathology of lung, liver, kidneys, and regional lymph nodes ensured the biomaterials biocompatibility. rESULTS: SSI remained negative throughout and independently from treatment. Differences between treted groups in the severity of changes in WrL existed, showing a faster regeneration for PVA-cNTs-MScs(P cute ankle joint angles were seen for PVA-cNTs-MScs group(P = 0.051) suggesting improved ankle muscles function during the push off phase of the gait cycle. In PVA-PPy and PVA-cNTs groups, there was a 25% and 42% increase of average fiber area and a 13% and 21% increase of the "minimal Feret's diameter" respectively. Stereological analysis disclosed a significantly(P creased myelin thickness(M), ratio myelin thickness/axon diameter(M/d) and ratio axon diameter/fiber diameter(d/D; g-ratio) in PVA-cNT-MScs group(P cONcLUSION: results revealed that treatment with MScs and PVA-cNTs tube-guides induced better nerve fiber regeneration. Functional and kinematics analysis revealed positive synergistic effects brought by MScs and PVA-cNTs. The PVA-cNTs and PVA-PPy are promising scaffolds with electric conductive properties, bio- and cytocompatible th

关键词： Nerve regeneration Neurotmesis Tubeguides Mesenchymal stem cells carbon nanotubes Functional assessment Gait biomechanical analysis Histomorfometry Polyvinyl alcohol

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Phase Ⅰ of cardiac rehabilitation:A new challenge for evidence based physiotherapy

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World Journal of cardiology 2011年第7期3卷 248-255页

作者： rafael Michel de Macedo Josérocha Faria-Neto costantino Ortiz costantini Dayane casali Andrea pires Muller costantino roberto costantini Katherine Athayde Teixeira de carvalho Luiz césar Guarita-Souza Department of rehabilitation Costantini Cardiological HospitalCuritiba80320-320Brazil Graduate Program in Health Sciences Catholic Pontificia University of ParanáCuritiba80215-901Brazil Graduate Program in Biotechnology Applied to Health Pequeno Príncipe FacultyCuritiba80230-020Brazil

cardiac rehabilitation protocols applied during the inhospital phase(phaseⅠ)are subjective and their results are contested when evaluated considering what should be the three basic principles of exercise prescription:specificity,overload and *** this review,we focus on the problems associated with the models of exercise prescription applied at this early stage in-hospital and adopted today,especially the lack of clinical studies demonstrating its ***,we present the concept of"periodization"as a useful tool in the search for better results.

关键词： cardiac rehabilitation Exercise physiology Physical therapy

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Fungal diversity notes 253-366:taxonomic and phylogenetic contributions to fungal taxa

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Fungal Diversity 2016年第3期78卷 1-237页

作者： Guo Jie Li Kevin D.Hyde rui Lin Zhao Sinang Hongsanan Faten Awad Abdel-Aziz Mohamed A.Abdel-Wahab Pablo Alvarado Genivaldo Alves-Silva Joseph F.Ammirati Hiran A.Ariyawansa Abhishek Baghela Ali Hassan Bahkali Michael Beug D.Jayarama Bhat Dimitar Bojantchev Thitiya Boonpratuang Timur S.Bulgakov Erio camporesi Marcela cBoro Oldriska ceska Dyutiparna chakraborty Jia Jia chen K.W.Thilini chethana Putarak chomnunti Giovanni consiglio Bao Kai cui Dong Qin Dai Yu cheng Dai Dinushani A.Daranagama Kanad Das Monika c.Dayarathne Eske De crop rafael J.V.De Oliveira c.r.os Alberto Fragoso de Souza JoséIde Souza Bryn T.M.Dentinger Asha J.Dissanayake Mingkwan Doilom E.ricardo Drechsler-Santos Masoomeh Ghobad-Nejhad Sean P.Gilmore Aristóteles Góes-Neto MichałGorczak charles H.Haitjema Kalani Kanchana Hapuarachchi Akira Hashimoto Mao Qiang He John K.Henske Kazuyuki Hirayama Maria J.Iribarren Subashini c.Jayasiri ruvishika S.Jayawardena Sun Jeong Jeon Gustavo H.Jerônimo Ana L.Jesus E.B.Gareth Jones Ji chuan Kang Samantha c.Karunarathna Paul M.Kirk Sirinapa Konta Eric Kuhnert Ewald Langer Haeng Sub Lee Hyang Burm Lee Wen Jing Li Xing Hong Li Kare Liimatainen Diogo Xavier Lima chuan Gen Lin Jian Kui Liu Xings Zhong Liu Zuo Yi Liu J.Jennifer Luangsa-ard robert Lücking H.Thorsten Lumbsch Saisamorn Lumyong Eduardo M.Leaño Agostina V.Marano Misato Matsumura Eric H.c.McKenzie Suchada Mongkolsamrit Peter E.Mortimer Thi Thuong Thuong Nguyen Tuula Niskanen chada Norphanphoun Michelle A.O’Malley Sittiporn Parnmen Julia Pawłowska rekhani H.Perera rungtiwa Phookamsak chayanard Phukhamsakda carmen L.A.pires-Zottarelli Olivier raspé Mateus A.reck Sarah c.O.rocha AndréL.c.M.Ade Santiago Indunil c.Senanayake Ledo Setti Qiu Ju Shang Sanjay K.Singh Esteban B.Sir Kevin V.Solomon Jie Song Prasert Srikitikulchai Marc Stadler Satinee Suetrong Hayato Takahashi Takumasa Takahashi Kazuaki Tanaka Li Ping Tang Kasun M.Thambugala Donnaya Thanakitpipattana Michael K.Theodorou Benjarong Thongbai Tuksaporn Thummarukcharoen Qing Tian Saowaluck Tibpromma Annemieke State Key Laboratory of Mycology Institute of MicrobiologyChinese Academy of SciencesNo 31st West Beichen RoadChaoyang DistrictBeijing 100101People’s Republic of China centre of Excellence in Fungal research and School of Science Mae Fah Luang UniversityChiang Rai 57100Thailand Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of ScienceKunming 650201YunnanPeople’s Republic of China Department of Botany and Microbiology College of ScienceKing Saud UniversityP.O.Box:2455Riyadh 1145Saudi Arabia Department of Botany and Microbiology Faculty of ScienceSohag UniversitySohag 82524Egypt ALVALAB C/La Rochela nº47E-39012 SantanderSpain Micolab Programa de Pós-Graduação em Biologia de FungosAlgas e PlantasDepartamento de BotânicaUniversidade Federal de Santa CatarinaCampus Universitário TrindadeCEP:88040-900 FlorianópolisSanta CatarinaBrazil Department of Biology University of WashingtonBox 351800SeattleWA 98195-1800USA Guizhou Key Laboratory of Agricultural Biotechnology Guizhou Academy of Agricultural SciencesGuiyang 550006GuizhouPeople’s Republic of China National Fungal culture collection of India(NFccI) Biodiversity and Palaeobiology GroupMACS’Agharkar Research InstituteG.G.Agarkar RoadPune 411004India PO Box 116 HusumWA 98623-0116USA Formerly Department of BotanyGoa UniversityGoaIndia No.128/1-J Azad Housing SocietyCurcaP.O.Goa Velha 403108India *** 345 Shipwatch LaneHerculesCA 94547USA Microbe Interaction Laboratory(BMIT) BIOTECNational Science and Technology Development Agency(NSTDA)113 Thailand Science ParkThanon PhahonyothinTombon Khlong NuengAmphoeKhlong LuangPathum Thani 12120Thailand Academy of Biology and Biotechnology Southern Federal UniversityRostov-on-Don 344090Rostov regionRussia A.M.B.Gruppo Micologico Forlivese BAntonio“cicognani” Via Roma Forlì Italy 18 A.M.B.Circolo Micologico“Giovanni Carini”C.P.314BresciaItaly Societàper gli Studi Naturalistici della romagna C.P.144Bagnacavallo

Notes on 113 fungal taxa are compiled in this paper,including 11 new genera,89 new species,one new subspecies,three new combinations and seven reference *** geographic and taxonomic range of fungal taxa are *** the Ascomycota the new genera Angustospora(Testudinaceae),camporesia(Xylariaceae),clematidis,crassiparies(Pleosporales genera incertae sedis),Farasanispora,Longiostiolum(Pleosporales genera incertae sedis),Multilocularia(Parabambusicolaceae),Neophaeocryptopus(Dothideaceae),Parameliola(Pleosporales genera incertae sedis),and Towyspora(Lentitheciaceae)are *** introduced species are Angustospora nilensis,Aniptodera aquibella,Annulohypoxylon albidiscum,Astrocystis thailandica,camporesia sambuci,clematidis italica,colletotrichum menispermi,***,comoclathris pimpinellae,crassiparies quadrisporus,cytospora salicicola,Diatrype thailandica,Dothiorella rhamni,Durotheca macrostroma,Farasanispora avicenniae,Halorosellinia rhizophorae,Humicola koreana,Hypoxylon lilloi,Kirschsteiniothelia tectonae,Lindgomyces okinawaensis,Longiostiolum tectonae,Lophiostoma pseudoarmatisporum,Moelleriella phukhiaoensis,***,Mucoharknessia anthoxanthi,Multilocularia bambusae,Multiseptospora thysanolaenae,Neophaeocryptopus cytisi,Ocellularia arachchigei,***,Ochronectria thailandica,Ophiocordyceps karstii,Parameliola acaciae,***,Parastagonospora cumpignensis,Pseudodidymosphaeria phlei,Polyplosphaeria thailandica,Pseudolachnella brevifusiformis,Psiloglonium macrosporum,rhabdodiscus albodenticulatus,rosellinia chiangmaiensis,Saccothecium rubi,Seimatosporium pseudocornii,***,Sigarispora ononidis and Towyspora *** combinations are provided for Eutiarosporella dactylidis(sexual morph described and illus trated)and Pseudocamarosporium ***,illustrations and/or reference specimens are designated for Aposphaeria corallinolutea,cryptovalsa ampelina,Dothiorella vidmadera,Ophiocordyceps formosana,P

关键词： Ascomycota Basidiomycota Neocallimastigomycota Oomycota.Zygomycota Phylogeny Taxonomy New genus New species

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DOTAP脂质体介导VEGF基因转染人脂肪干细胞及目的基因的表达

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临床耳鼻咽喉头颈外科杂志 2016年第12期30卷 966-971页

作者：陈犹白张启旭 Butler c E 吴叶文张丽萍董江陵陈聪慧韩岩美国德克萨斯大学MD安德森肿瘤中心整形外科休斯敦77030 解放军总医院整形修复科北京100853 美国贝勒医学院医学系肾脏学部北京美莱美容整形医院口腔美容中心

目的：研究脂质体介导的血管内皮生长因子（VEGF）基因转染人脂肪干细胞（hAScs）及VEGF的表达。方法：胶原酶消化吸脂术脂肪,收获细胞沉淀,培养至第4代时利用流式细胞术检测细胞表型,并诱导其成脂成骨分化,鉴定为hAScs。转染组利用DOTA... 详细信息

目的：研究脂质体介导的血管内皮生长因子（VEGF）基因转染人脂肪干细胞（hAScs）及VEGF的表达。方法：胶原酶消化吸脂术脂肪,收获细胞沉淀,培养至第4代时利用流式细胞术检测细胞表型,并诱导其成脂成骨分化,鉴定为hAScs。转染组利用DOTAP脂质体将pires2-EGFP-VEGF质粒转入hAScs,对照组为脂质体空载。免疫荧光染色检测hAScs内VEGF的表达,ELISA检测上清液中VEGF浓度的变化。结果：1ml吸脂术脂肪可得到（4.38±0.21）×10^5个细胞,第4代时hAScs高表达cD90（81.49%）,低表达cD19（6.37%）、cD31（14.91%）、cD34（17.56%）和cD45（15.39%）,诱导分化后油红O和茜素红染色均呈阳性。分光光度计显示质粒DNA浓度为595ng/μl。转染组hAScs表达GFP和VEGF,转染效率为（43.69±18.53）%;对照组不表达GFP,但表达较低的VEGF。转染组VEGF的光密度是对照组的2.13倍,其上清液中的VEGF浓度随时间显著上升,与对照组相比差异有统计学意义（P〈0.05）。结论：DOTAP脂质体可成功将pires2-EGFP-VEGF质粒转入hAScs,转染后的VEGF表达和分泌水平显著提高。

关键词：吸脂术脂肪干细胞血管内皮生长因子转染脂质体

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Studies on coexpression of human BMP-2 and OPG and the feasibility of gene treatment for osteoporosis

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中国临床康复 2002年第20期6卷 3130-3131页

作者： Liu J.-Z. Hu Y.-Y. Ji Z.-L. chen S.-M. Tao H.-r. Institute of Orthopedic Surgery Xijing Hospital Xi'an 710032 China Department of Biochemistry Molecular Biology Fourth Military Medical University Xi'an 710032 China

Objectives To construct human BMP-2 and OPG coexpressing vector and determine its expression in mannalian cells in vitro. Methods Using the isolated total rNA from osteosacoma cell line MG63 as a template,the cDNA encoding hOPG was amplified by reverse transcription-polymerase chain reation (rT-Pcr) method and cloned into mammalian expression vector pires2-EGFP endonucleases sites Ecor I and BamH I, and the BMP-2 encoding region gene was cloned into endonucleases site BstX *** the recombinant plasmid pires2-OPG-BMP2 was transformed into cos-7 cell line,OPG and BMP-2 expression were determined by Western blot *** (1) The sequence of OPG cDNA obtained in this experiment was the same as that of reported, recombinant plasmid pires-BMP2-OPG was constructed successfully. (2) Human OPG and BMP-2 overexpression cell line cos-7 was selected and confirmed by Western blot analysis .conclusion Human OPG encoding *** and BMP-2 coexpressing vector and cell lineage expressed OPG and BMP-2 were obtained. These datas suggested coexpressing human OPG and BMP-2 may be a promising and logical apporach for treatment of osteoporosis.

关键词：骨质疏松基因治疗 BMP2 OPG 真核载体

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IGF-1和动态微环境对脂肪干细胞向心肌细胞分化作用的研究

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生物化学与生物物理进展 2009年第12期36卷 1553-1561页

作者：朱艳霞刘天庆宋克东马学虎崔占峰大连理工大学干细胞与组织工程研发中心大连116024 Institute of Biomedical Engineering Department of Engineering ScienceOxford University

体外组织工程模型中,生物化学和机械信号对心肌再生起着很重要的促进作用,对人胰岛素样生长因子(IGF-1)和三维动态微环境对脂肪干细胞向心肌细胞分化过程中的促进作用进行了研究.带有IGF-1基因的质粒整合到胶原-壳聚糖支架中,脂肪干细... 详细信息

体外组织工程模型中,生物化学和机械信号对心肌再生起着很重要的促进作用,对人胰岛素样生长因子(IGF-1)和三维动态微环境对脂肪干细胞向心肌细胞分化过程中的促进作用进行了研究.带有IGF-1基因的质粒整合到胶原-壳聚糖支架中,脂肪干细胞接种到整合质粒的支架内,未整合质粒的支架作为对照组,心肌细胞培养基作为分化培养基,转瓶生物反应器提供动态微环境.经2周分化培养后,检测质粒在支架内释放及表达情况、细胞在支架内的活性以及心肌功能性蛋白和基因的表达.结果表明:动态微环境能促进质粒DNA的释放和转染;IGF-1可促进脂肪干细胞在胶原-壳聚糖支架内增殖以及向心肌细胞分化;动态微环境可加强IGF-1的促增殖分化作用.因此,IGF-1和动态微环境能独立或相互促进脂肪干细胞在胶原-壳聚糖支架内活性,动态微环境还可强化IGF-1对脂肪干细胞的促分化作用.对体外构建工程化心肌组织进行心肌再生研究有着重要的指导意义.

关键词：脂肪干细胞心肌细胞支架 IGF-1 反应器组织工程

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