检索结果-南通市图书馆

Particuology 2019年第5期17卷 22-29页

作者： M. Hafizur Rahman Lius Daniel Ujash Shah Xiaotao Bi John R. Grace c.jim lim Fluidization Research centre Department of Chemical & Biological Engineering University of British Columbia 2360 East Mall Vancouver British Columbia V6T1Z3 Canada

Operation of a dual fluidized bed, consisting of a riser as combustor and bubbling bed as gasifier, for synthesis gas production from a solid fuel requires determination of the solids circulation rate and char transfer rate. The performance relies on supplying sufficient heat from the combustor to the gasifier by circulation of solids between these two reactors. The flow rate of char is required to track the heat generated in the combustor, which supports endothermic reactions in the gasifier. Direct measurement of these two critical parameters is difficult, with the number of reported techniques capable of working at high temperatures extremely small. An indirect method was developed, using mass and energy balances over the entire system and individual reactors, to estimate the solids circulation rate and char transfer rate. There was general agreement between heat losses estimated from energy-balance calculations and from direct measurement of the outer reactor surface temperature. Under typical gasification conditions, the solids circulation fluxes were estimated to be 45.2 and 55.6 kg/(m^2 s) in two independent tests, which were in good agreement with values obtained using a thermal tracer;char transfer rates were calculated to be 1.2 and 0.6 kg/h, which were in reasonable agreement with average biomass feed rates. This method can be applied to dual gasification systems at any temperature or flow rate.

关键词： Solids circulation rate char transfer rate Pilot-plant gasifier Dual fluidized bed Mass and energy balance

Single-cell and spatial omics:exploring hypothalamic heterogeneity

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第6期20卷 1525-1540页

作者： Muhammad Junaid Eun Jeong Lee Su Bin lim Department of Biochemistry&Molecular Biology Ajou University School of MedicineSuwonSouth Korea Department of Biomedical Sciences Graduate School of Ajou UniversitySuwonSouth Korea Department of Brain Science Ajou University School of MedicineSuwonSouth Korea

Elucidating the complex dynamic cellular organization in the hypothalamus is critical for understanding its role in coordinating fundamental body functions. Over the past decade, single-cell and spatial omics technologies have significantly evolved, overcoming initial technical challenges in capturing and analyzing individual cells. These high-throughput omics technologies now offer a remarkable opportunity to comprehend the complex spatiotemporal patterns of transcriptional diversity and cell-type characteristics across the entire hypothalamus. current single-cell and single-nucleus RNA sequencing methods comprehensively quantify gene expression by exploring distinct phenotypes across various subregions of the hypothalamus. However, single-cell/single-nucleus RNA sequencing requires isolating the cell/nuclei from the tissue, potentially resulting in the loss of spatial information concerning neuronal networks. Spatial transcriptomics methods, by bypassing the cell dissociation, can elucidate the intricate spatial organization of neural networks through their imaging and sequencing technologies. In this review, we highlight the applicative value of single-cell and spatial transcriptomics in exploring the complex molecular-genetic diversity of hypothalamic cell types, driven by recent high-throughput achievements.

关键词： cellular diversity hypothalamus multi-omics single-cell transcriptomics spatial transcriptomics

Biomass torrefaction in a slot-rectangular spouted bed reactor

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Particuology 2019年第1期17卷 154-162页

作者： Ziliang Wang c. jim lim John R. Grace Department of chemical and Biological Engineering. University of British columbia Vancouver. Canada

Sawdust was subjected to torrefaction in a "semi-batch" slot-rectangular spouted bed (SRSB) reactor at temperatures from 240 to 330℃ and biomass feed rates from 220 to 710g/*** spouting of the sawdust was achieved in the slot-rectangular spouted bed,although the pressure drop across the reactor was observed to *** to the biomass feed rate,the temperature had a greater effect on the biomass weight loss and energy *** in temperature were found to promote weight loss of the sawdust while decreasing the energy *** main solid product was the torrefied sawdust,which remained in the SRSB reactor and was captured by a *** ratio of the torrefied biomass removed by the cyclone to the total torrefied biomass increased along with both the feed rate and *** undergoing torrefaction,6.7%-39.2% of the original sawdust mass was lost while 67.4%-98.7% of its energy was *** torrefied sawdust had a higher carbon content but less oxygen,hydrogen and volatiles,along with a greater higher heating value and increased density compared to the raw *** size of the sawdust particles also decreased markedly during the torrefaction process.

关键词： Torrefaction Slot-rectangular spouted bed reactor Temperature Biomass feed rate Torrefied sawdust property

comprehensive molecular characterization to predict immunotherapy response in advanced biliary tract cancer:a phase II trial of pembrolizumab

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Oncology Research 2025年第1期33卷 57-65页

作者： RYUL KIM JOO KYUNG PARK MINSUK KWON MINAE AN JUNG YONG HONG JOON OH PARK SUNG HEE lim SEUNG TAE KIM Division of Hematology-Oncology Department of MedicineSamsung Medical CenterSungkyunkwan University School of MedicineSeoulKorea Division of Gastroenterology Department of MedicineSamsung Medical CenterSungkyunkwan University School of MedicineSeoulKorea Department of Hematology-Oncology Ajou University School of MedicineSuwon-siGyeonggi-doKorea Samsung Advanced Institute of Health Science and Technology Sungkyunkwan University School of MedicineSeoulKorea

Background:Immune checkpoint inhibitors(IcIs)are effective in a subset of patients with metastatic solid ***,the patients who would benefit most from IcIs in biliary tract cancer(BTc)are still *** and methods:We molecularly characterized tissues and blood from 32 patients with metastatic BTc treated with the IcI pembrolizumab as second-line ***:All patients had microsatellite stable(MSS)type *** of the 32 patients achieved partial response(PR),with an objective response rate(ORR)of 9.4%(95%confidence interval[cI],2.0–25.2)and nine showed stable disease(SD),exhibiting a disease control rate(DcR)of 37.5%(95%cI,21.1–56.3).For the 31 patients who had access to PD-1 ligand 1(PD-L1)combined positive score(cPS)testing(cut-off value≥1%),the ORR was not different between those who had PD-L1-positive(PD-L1+;1/11,9.1%)and PDL1-(2/20,10.0%)tumors(p=1.000).The tumor mutational burden(TMB)of PD-L1+BTc was comparable to that of PD-L1-BTc(p=0.630).TMB and any exonic somatic mutations were also not predictive of pembrolizumab *** analysis of blood and tumor samples demonstrated a relatively high natural killer(NK)cell proportion in the peripheral blood before pembrolizumab treatment in patients who achieved tumor ***,the tumors of these patients presented high enrichment scores for NK cells,antitumor cytokines,and Th1 signatures,and a low enrichment score for cancer-associated ***:This study shows the molecular characteristics associated with the efficacy of pembrolizumab in BTc of the MSS type.

关键词： Pembrolizumab Whole-exome sequencing Whole-transcriptome sequencing Biliary tract cancer

Extended protective effects of three dimensional cultured human mesenchymal stromal cells in a neuroinflammation model

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Stem cells 2025年第1期17卷 53-65页

作者： Ok-Hyeon Kim Hana Kang Eun Seo chang Younghyun lim Young-Jin Seo Hyun Jung Lee Department of Anatomy and cell Biology College of MedicineChung-Ang UniversitySeoul 06974South Korea Department of Global Innovative Drugs Graduate School of Chung-Ang UniversitySeoul 06974South Korea Department of Life Science Chung-Ang UniversitySeoul 06974South Korea

BAcKGROUND Human mesenchymal stromal cells(MScs)possess regenerative potential due to pluripotency and paracrine ***,their stemness and immunomod-ulatory capabilities are sub-optimal in conventional two-dimensional(2D)*** To enhance the efficiency and therapeutic efficacy of MScs,an in vivo-like 3D culture condition was *** MScs were cultured on polystyrene(2D)or in a gellan gum-based 3D *** vitro,prostaglandin-endoperoxide synthase 2,indoleamine-2,3-dioxygenase,heme oxygenase 1,and prostaglandin E synthase gene expression was quantified by quantitative real-time polymerase chain *** were incubated with lipopolysaccharide(LPS)-treated mouse splenocytes,and prostaglandin E2 and tumor necrosis factor-alpha levels were measured by enzyme linked immuno-sorbent *** vivo,LPS was injected into the lateral ventricle of mouse brain,and MScs were administered intravenously the next *** were sacrificed and analyzed on days 2 and *** Gellan gum polymer-based 3D culture significantly increased expression of octamer-binding transcription factor 4 and Nanog homeobox stemness markers in human MScs compared to 2D *** 3D environment also heightened expression of cyclooxygenase-2 and heme-oxygenase 1,enzymes known for immunomodulatory functions,including production of prostaglandins and heme degradation,*** in 3D culture secreted more prostaglandin E2 and effectively suppressed tumor necrosis factor-alpha release from LPS-stimulated splenocytes and surpassed the efficiency of MScs cultured in *** a murine neuroinflammation model,intravenous injection of 3D-cultured MScs significantly reduced ionized calcium-binding adaptor molecule 1 and glial fibrillary acidic protein expression,mitigating chronic inflammation more effectively than 2D-cultured *** The microenvironment established in 3D culture serves as an in vivo mimetic,enhancing the immunomodulatory function of *** sugge

关键词： Mesenchymal stromal cells Three-dimensional culture Immunomodulatory function Neuroinflammation cell therapy

Heat transfer in a tapered fluidized bed of biomass particles with pulsed gas flow

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Particuology 2019年第1期17卷 2-14页

作者： Dening Jia Xiaotao Bi c. jim lim Shahab Sokhansanj Atsushi Tsutsumi Department of chemical and Biological Engineering The University of British Columbia2360 East MallVancouverBCV6T 1Z3Canada Environmental Sciences Division Oak Ridge National LaboratoryOak RidgeTN 37831USA collaborative Research center for Energy Engineering Institute of Industrial ScienceThe University of Tokyo4-6-1 KomabaMeguro-kuTokyo 153-8505Japan

Bed-to-surface heat transfer of pure biomass particles in a pulsed fluidized bed with a tapered bottom section was investigated. Three biomass species — Douglas fir, pine, and switchgrass — were studied under various operating conditions. Their heat transfer coefficients were found to be closely associated with hydrodynamics dominated by gas pulsations. A higher superficial gas velocity generally yielded better gas–solid contact and higher heat transfer rates. A moderately increasing pulsation frequency promoted convective heat transfer of particles but also reduced pulsation intensity, leading to undesired flow behaviours such as channelling and partial defluidization. The study of the pulsation duty cycle revealed that, for cohesive particles, a smaller duty cycle was preferred to generate powerful pulsations to break up inter-particle forces. Moreover, a duty cycle increase allowed higher gas throughput as long as a suitable fluidization was maintained. The addition of finer particles to a coarse fraction increased particle mobility, and subsequently heat transfer, which also explained the higher heat transfer coefficients of switchgrass as it contained more fines compared with fir and pine. Experimental results in the tapered bed were also compared with those of non-tapered geometry where a 10%–20% increase in heat transfer was observed.

关键词： Fluidized bed Gas pulsations Tapered column Heat transfer Biomass

Effects of the mass and volume shrinkage of ground chip and pellet particles on drying rates

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Particuology 2018年第3期16卷 1-9页

作者： Hamid Rezaei Shahab Sokhansaj c. jim lim Anthony Lau Xiaotao Bi chemical and Biological Engineering Department University of British Columbia Vancouver BC V6T 1Z3 Canada Environmental Science Division Oak Ridge National Laboratory Oak Ridge TN 37381 USA

The effects of varying the mass and volume of ground chip and pellet particles on the particle drying rate were analyzed. Samples of whole pellets and chips were hammer milled using a 3.2 mm screen and the ground chip and pellet particles were found to have similar size distributions, although the pellet particles were denser and more spherical than the chip particles. Prior to drying, water was added to the particles to obtain 0.10, 0.30, 0.50, 0.70, and 0.90 moisture contents （on a dry mass basis）. The moistened particles were subsequently dried in a constant temperature thin layer dryer set at 50,100, 150, or 200 ~c under dry pure nitrogen, dry compressed air, or atmospheric air. The chip and pellet particles exhibited similar degrees of shrinkage, but the pellet particles underwent a greater reduction in their bulk volume during drying. It appears that the more spherical pellet particles are prone to shrinkage in more than one direction, whereas the needle-like chip particle shrink only in one direction. A variable radius first order drying model was found to fit the experimental data better than a fixed radius model.

关键词： Moisture loss Particle density Ground chip Ground pellet Particle shrinkage Bulk shrinkage

TMEM39A and TMEM131 facilitate bulk transport of EcM proteins through large cOPII vesicle formation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2025年第2期52卷 189-203页

作者： Jee Young Sung Ga-Eun lim Jarim Goo Kyung Jin Jung Jeong Min chung Hyun Suk Jung Yong-Nyun Kim Jaegal Shim cancer Metastasis Branch Research InstituteNational Cancer Center323 Ilsan-roGoyang-siGyeonggi-do10408Republic of Korea Experimental clinical Research center Biomedical Research InstituteSeoul National University Bundang HospitalSeongnamGyeonggi-do13620Republic of Korea Department of Biochemistry College of Natural SciencesKangwon National UniversityChuncheonKangwon-do24341Republic of Korea

The growth of caenorhabditis elegans involves multiple molting processes,during which old cuticles are shed and new cuticles are rapidly *** process requires the regulated bulk secretion of cuticle *** transmembrane protein-39(TMEM-39)mutant exhibits distinct dumpy and ruptured phenotypes characterized by notably thin ***-39 primarily co-localizes with the coat protein II complex(cOPII)in large vesicles rather than small cOPII *** TMEM-39-associated large vesicles(TMEM-39-LVs)form robustly during the molting period and co-localize with various extracellular matrix components,including BLI-1 collagen,BLI-3 dual oxidase,and *** immunoprecipitation using TMEM39A-FLAG and proteomics analysis in human sarcoma cells,we identify TMEM39A-associated proteins,including *** of TMEM131 results in reduced TMEM39A-LV formation and collagen secretion in both *** and human sarcoma cells,indicating a cooperative role between TMEM39A and TMEM131 in the secretion of extracellular components through the formation of large cOPII *** the conservation of TMEM39A and its associated proteins between *** and humans,TMEM39A-LVs may represent a fundamental machinery for rapid and extensive secretion across metazoans.

关键词： Bulk secretion collagen cOPII EcM TMEM39A TMEM131

Immortalization of epidural fat-derived mesenchymal stem cells: In vitro characterization and adipocyte differentiation potential

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Stem cells 2025年第1期17卷 40-52页

作者： Seoung-Woo Lee Young-Ju lim Hee-Yeon Kim Wansoo Kim Wook-Tae Park Min-Jung Ma Junho Lee Min-Soo Seo Young In Kim Sangbum Park Seong-Kyoon choi Gun Woo Lee core Protein Resources center Daegu-Gyeongbuk Institute of Science and TechnologyDaegu 42988South Korea Department of Orthopedic Surgery Yeungnam University Medical CenterYeungnam University College of MedicineDaegu 42415South Korea Department of Veterinary Pathology College of Veterinary MedicineKyungpook National UniversityDaegu 41566South Korea School of Life Science BK21 FOUR KNU Creative BioResearch GroupKyungpook National UniversityDaegu 41566South Korea Laboratory of Veterinary Tissue Engineering College of Veterinary MedicineKyungpook National UniversityDaegu 41566South Korea college of Veterinary Medicine Kyungpook National UniversityDaegu 41566South Korea Department of Veterinary Tissue Engineering Kyungpook National UniversityDaegu 41566South Korea cellexobio. Ltd.Daegu 42415South Korea Michigan State University Institute for Quantitative Health Science&EngineeringEast LansingMI 48824United States Department of Orthopedic Surgery Yeungnam University College of MedicineDaegu 42415South Korea

BAcKGROUND Mesenchymal stem cells(MScs)are promising candidates for regenerative therapy due to their self-renewal capability,multilineage differentiation potential,and immunomodulatory *** molecular characteristics of MScs are influenced by their ***,epidural fat(EF)and EF-derived MScs(EF-MScs)have garnered attention due to their potential benefits to the spinal microenvironment and their high expression of neural Sc ***,their clinical applications are limited due to cell senescence and limited accessibility of *** many studies have attempted to establish an immortalized,stable Sc line,the characteristics of immortalized EF-MScs remain to be *** To establish and analyze stable immortalized *** The phenotypes of EF-MScs were analyzed using optical *** immortalization was performed using lentiviral *** biomolecular characteristics of the cells were analyzed by immunoblotting,quantitative PcR,and *** The immortalized EF-MScs demonstrated a significantly extended lifespan compared to the control group,with well-preserved adipogenic potential and Sc surface marker *** of human telomerase reverse transcriptase genes markedly increased the lifespan of *** analysis revealed substantial increase in the expression of DNA replication pathway components in immortalized *** Immortalized EF-MScs exhibited significantly enhanced proliferative capacity,retained adipogenic potential,and upregulated the expression of DNA replication pathway components.

关键词： Immortalization Transfection Stem cell Epidural fat Proteomics

On the truth of nanoscale for nanobeams based on nonlocal elastic stress field theory:equilibrium,governing equation and static deflection

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Applied Mathematics and Mechanics(English Edition) 2010年第1期31卷 37-54页

作者： c. W. lim Department of Building and construction City University of Hong Kong

This paper has successfully addressed three critical but overlooked issues in nonlocal elastic stress field theory for nanobeams：（i） why does the presence of increasing nonlocal effects induce reduced nanostructural stiffness in many, but not consistently for all, cases of study, i.e., increasing static deflection, decreasing natural frequency and decreasing buckling load, although physical intuition according to the nonlocal elasticity field theory first established by Eringen tells otherwise？（ii） the intriguing conclusion that nanoscale effects are missing in the solutions in many exemplary cases of study, e.g., bending deflection of a cantilever nanobeam with a point load at its tip; and （iii） the non-existence of additional higher-order boundary conditions for a higher-order governing differential equation. Applying the nonlocal elasticity field theory in nanomechanics and an exact variational principal approach, we derive the new equilibrium conditions, do- main governing differential equation and boundary conditions for bending of nanobeams. These equations and conditions involve essential higher-order differential terms which are opposite in sign with respect to the previously studies in the statics and dynamics of nonlocal nano-structures. The difference in higher-order terms results in reverse trends of nanoscale effects with respect to the conclusion of this paper. Effectively, this paper reports new equilibrium conditions, governing differential equation and boundary condi- tions and the true basic static responses for bending of nanobeams. It is also concluded that the widely accepted equilibrium conditions of nonlocal nanostructures are in fact not in equilibrium, but they can be made perfect should the nonlocal bending moment be replaced by an effective nonlocal bending moment. These conclusions are substantiated, in a general sense, by other approaches in nanostructural models such as strain gradient theory, modified couple stress models and experim

关键词： bending effective nonlocal bending moment nanobeam nanomechanics,nanoscale nonlocal elastic stress strain gradient