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silvestrol alleviates glioblastoma progression through ERK pathway modulation and MANBA and NRG-1 expression

BIOcELL 2024年第7期48卷 1081-1093页

作者： LAN ZHOU QI ZHANG BO TIAN FENG yang Neurosurgery Department The First Affiliated Hospital of Chongqing Medical and Pharmaceutical CollegeChongqingChina Neurosurgery Department Changdu People’s Hospital of TibetChangduChina

Background:Glioblastoma,a notably malignant tumor within the central nervous system,is distinguished by its aggressive ***,a robust inhibitor of the RNA helic.s. eukaryotic initiation factor 4A(eIF4A),has shown significant potential as an anticancer ***,the impact of silvestrol on glioblastoma,especially its molecular mechanisms,has not been fully ***:This investigation employed a variety of in vitro assays,such as cell counting kit-8(ccK-8),clonogenic,5-ethynyl-2′-deoxyuridine(EDU),wound healing,and flow cytometry,to evaluate cell cycle progression,apoptosis,cell viability,and *** blot analysis was also performed to study the apoptosis and extracellular regulated kinase(ERK)*** the ERK pathway was inhibited,differentially expressed genes(DEGs)in U87 cells were identified,followed by an analysis of target genes using the gene expression profiling interactive analysis(GEPIA)***:silvestrol significantly suppressed the proliferation,migration,and colony formation of glioma *** caused cell cycle arrest and enhanced apoptosis in these ***,silvestrol stimulated the ERK pathway,with these effec.s.being reversible by an ERK phosphorylation *** combined with GEPIA,GscA,UALcAN,TIMER database screened 4 potential drug targets of silvestrol:chromosome 1 open reading frame 226(c1ORF226),mannosidase beta A(MANBA),IQ motif and sec7 domain 2(IQsEc2),neuregulin 1(NRG-1).Among them,c1ORF226 was lower risk gene while MANBA,IQsEc2,and NRG-1 were high-risk ***,silvestrol notably reduced MANBA mRNA levels,which could be reversed by inhibiting ERK ***,silvestrol markedly decreased NRG-1 protein levels,with an additional reduction observed when the ERK pathway was ***:silvestrol’s anti-glioma effec.s.are primarily due to the suppression of MANBA expression via the ERK pathway and possibly by hindering the translation of N

关键词： Glioblastoma silvestrol ERK pathway MANBA NRG-1

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The soft X-ray Imager(sXI)on the sMILE Mission

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Earth and Planetary Physics 2024年第1期8卷 5-14页

作者： s.sembay A.L.Alme D.Agnolon T.Arnold A.Beardmore A.Belén Balado Margeli c.Bicknell c.Bouldin G.Branduardi-Raymont T.crawford J.P.Breuer T.Buggey G.Butcher R.canchal J.A.carter A.cheney Y.collado-Vega H.connor T.crawford N.Eaton c.Feldman c.Forsyth T.Frantzen G.Galgóczi J.Garcia G.Y.Genov c.Gordillo H-P.Gröbelbauer M.Guedel Y.Guo M.Hailey D.Hall R.Hampson J.Hasiba O.Hetherington A.Holland s-Y.Hsieh M.W.J.Hubbard H.Jeszenszky M.Jones T.Kennedy K.Koch-Mehrin s.Kögl s.Krucker K.D.Kuntz c.Lakin G.Laky O.Lylund A.Martindale J.Miguel Mas Hesse R.Nakamura K.Oksavik N.Østgaard H.Ottacher R.Ottensamer c.Pagani s.Parsons P.Patel J.Pearson G.Peikert F.s.Porter T.Pouliantis B.H.Qureshi W.Raab G.Randal A.M.Read N.M.M.Roque M.E.Rostad c.Runciman s.sachdev A.samsonov M.soman D.sibeck s.smit J.søndergaard R.speight s.stavland M.steller TianRan sun J.Thornhill W.Thomas K.Ullaland B.Walsh D.Walton c.Wang s.yang school of Physics and Astronomy University of LeicesterLeicesterLE17RHUK space Park Leic.s.er University of LeicesterLeicesterLE17RHUK Mullard space science Laboratory University College LondonDorkingRH56NTUK centre for Electronic Imaging Open UniversityMilton KeynesMK76 AAUK Birkeland centre for space science Department of Physics and TechnologyUniv.of BergenN-5007 BergenNorway University of Vienna A-1010 ViennaAustria space Research Institute Austrian Academy of SciencesA-8042 GrazAustria National Institute of Aerospace Technology MadridSpain EsTEc European Space Agency2201 AZ NoordwijkNetherlands state Key Laboratory of space Weather National Space Science CentreBeijing 100190China University of Applied scienc.s.and Arts Northwestern switzerland CH-5210 WindischSwitzerland Koegl space GmbH CH-8157 DielsdorfSwitzerland space Acoustics GmbH CH-8157 DielsdorfSwitzerland Zurich University of Applied sciences 8400 WinterhurSwitzerland Genov solutions N-5172 LoddefjordNorway sTM Engineering N-5165 LaksevagNorway John Hopkins University Applied Physics Laboratory MD 20723USA NAsA/Goddard space Flight centre MD 20771USA center for space Physics Boston UniversityBostonMA 02215USA Department of Theoretical Physics and Astrophysics Masaryk University60177 BrnoCzech Republic Institute of Physics E¨otvo¨s Lor´and University1053 BudapestHungary Aerospace Information Research Institute Chinese Academy of SciencesBeijing 100045China centro de Astrobiologia(cAB) CSIC-INTAMadridSpain

The soft X-ray Imager(sXI)is part of the scientific payload of the solar wind Magnetosphere Ionosphere Link Explorer(sMILE)*** is a joint science mission between the European space Agency(EsA)and the chinese Academy of scienc.s.c.s.and is due for launch in *** is a compact X-ray telescope with a wide field-of-view(FOV)capable of encompassing large portions of Earth’s magnetosphere from the vantage point of the sMILE *** is sensitive to the soft X-rays produced by the solar Wind charge eXchange(sWcX)proc.s. produced when heavy ions of solar wind origin interact with neutral particles in Earth’s *** provides a mechanism for boundary detection within the magnetosphere,such as the position of Earth’s magnetopause,because the solar wind heavy ions have a very low density in regions of closed magnetic field *** sensitivity of the sXI is such that it can potentially track movements of the magnetopause on timescales of a few minutes and the orbit of sMILE will enable such movements to be tracked for segments lasting many *** is led by the University of Leic.s.er in the United Kingdom(UK)with collaborating organisations on hardware,software and science support within the UK,Europe,china and the United states.

关键词： soft X-ray Imaging micropore optics large area ccD

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Impact of sex on the outcomes of deceased donor liver transplantation

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World Journal of Transplantation 2024年第1期14卷 120-129页

作者： Oya M Andacoglu Isabel s Dennahy Nicole c Mountz Luisa Wilschrey Arzu Oezcelik Division of Transplantation and Advanced Hepatobiliary surgery University of UtahSalt Lake CityUT 84112United States Department of surgery University of EssenEssen D-45122Germany Department of surgery The University of Oklahoma Health Sciences CenterOklahoma CityOK 73117United States Department of surgery University of Oklahoma College of MedicineOklahoma CityOK 73117United States

BAcKGROUND Data examining the impact of sex on liver transplant(LT)outcomes are *** is clear that further research into sex-related differenc.s.in transplant patients is nec.s.ary to identify areas for *** of these differenc.s.may help to identify specific areas of foc.s.to improve on the organ matching proc.s.,as well as the peri-and post-operative care of these *** To utilize data from a high-volume Eurotransplant center to compare characteristics of male and female patients undergoing liver transplant and assess association between sex-specific variables with short-and long-term post-transplant *** A retrospective review of the University of Essen’s transplant database was performed with collection of baseline patient characteristics,transplant-related data,and short-term *** of these data were made with shapiro-Wilk,Mann-Whitney U,χ2 and Bonferroni tests applied where appropriate.A P value ofs accepted as statistically *** Of the total 779 LT recipients,261(33.5%)were *** patients suffered higher incidenc.s.of acute liver failure and lower incidenc.s.of alcohol-related or viremic liver disease(P=0.001).Female patients were more likely to have received an organ from a female donor with a higher donor risk index score,and as a high urgency offer(all Pseline characteristics of male and female recipients were also significantly *** multivariate hazard regression analysis,recipient lab-Model for End-stage Liver Disease score and donor cause of death were associated with long-term outcomes in ***-operative diagnosis of hepatocellular carcinoma,age at time of listing,duration of surgery,and units transfused during surgery,were associated with long-term outcomes in *** of complications was associated with long-term outcomes in both *** survival was similar in both males and females;however,when stratified by age,females<50 y

关键词： Liver transplant Outcomes survival Peri-and post-operative care

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Naturally crosslinked Biocompatible carbonaceous Liquid Metal Aqueous Ink Printing Wearable Electronics for Multi-sensing and Energy Harvesting

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Nano-Micro Letters 2024年第8期16卷 148-168页

作者： King Yan chung Bingang Xu Di Tan Qingjun yang Zihua Li Hong Fu Nanotechnology center School of Fashion and TextilesThe Hong Kong Polytechnic UniversityHong Kong 999077People’s Republic of China Department of Mathematics and Information Technology The Education University of Hong KongHong KongPeople’s Republic of China

Achieving flexible electronics with comfort and durability comparable to traditional textiles is one of the ultimate pursuits of smart *** printing is desirable for e-textile development using a simple and inexpensive ***,fabricating high-performance atop textiles with good dispersity,stability,biocompatibility,and wearability for high-resolution,large-scale manufacturing,and practical applications has remained ***,waterbased multi-walled carbon nanotubes(MWcNTs)-decorated liquid metal(LM)inks are proposed with carbonaceous gallium–indium *** the assistance of biopolymers,the sodium alginate-encapsulated LM droplets contain high carboxyl groups which non-covalently crosslink with silk sericin-mediated MWcNTs.E-textile can be prepared subsequently via printing technique and natural waterproof triboelectric coating,enabling good flexibility,hydrophilicity,breathability,wearability,biocompatibility,conductivity,stability,and excellent versatility,without any artificial *** obtained e-textile can be used in various applications with designable patterns and ***-sensing applications of recognizing complex human motions,breathing,phonation,and pressure distribution are demonstrated with repeatable and reliable ***-powered and energy-harvesting capabilities are also presented by driving electronic devic.s.and lighting *** proof of concept,this work provides new opportunities in a scalable and sustainable way to develop novel wearable electronics and smart clothing for future commercial applications.

关键词： Biocompatible conductive ink Biopolymer E-textile carbonaceous liquid metal

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stress relaxation properties of calcium silicate hydrate:a molecular dynamics study

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Journal of Zhejiang University-science A(Applied Physics & Engineering) 2024年第2期25卷 97-115页

作者： Zhicheng GENG shengwen TANG yang WANG Hubao A Zhen HE Kai WU Lei WANG state Key Laboratory of Water Resourc.s.Engineering and Management Wuhan UniversityWuhan430072China Key Laboratory of Advanced civil Engineering Materials of Ministry of Education School of Materials Science and EngineeringTongji UniversityShanghai200092China college of Materials science and Engineering Xi’an University of Architecture and TechnologyXi’an710055China

The time-dependent viscoelastic response of cement-based materials to applied deformation is far from fully understood at the atomic *** silicate hydrate(c.s.H),the main hydration product of Portland cement,is responsible for the viscoelastic mechanism of cement-based *** this study,a molecular model of c.s.H was developed to explain the stress relaxation characteristics of c.s.H at different initial deformation states,ca/si ratios,temperatures,and water contents,which cannot be acc.s.ed *** stress relaxation of c.s.H occurs regardless of whether it is subjected to initial shear,tensile,or compressive deformation,and shows a heterogeneous *** plays a crucial role in the stress relaxation proc.s..A large ca/si ratio and high temperature reduce the cohesion between the calcium-silicate layer and the interlayer region,and the visc.s.ty of the interlayer region,thereby accelerating the stress relaxation of *** effect of the hydrogen bond network and the morphology of c.s.H on the evolution of the stress relaxation characteristics of c.s.H at different water contents was elucidated by nonaffine mean squared *** results shed light on the stress relaxation characteristics of c.s.H from a microscopic perspective,bridging the gap between the microscopic phenomena and the underlying atomic-level mechanisms.

关键词： calcium silicate hydrate(c.s.H) stress relaxation ca/si ratio Temperature Water content Atomic.s.mulation

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Immediate implantation of ultrafine fiber slow-release system based on cell electrospinning to induce osteogenesis of mesenchymal stem cells

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Regenerative Biomaterials 2024年第2期3卷 76-87页

作者： Tao Lu Long yang Zhuoyang Li Yin Liu shun'en Xu chuan Ye Department of Orthopaedics The Affliated Hospital of Guizhou Medical UniversityGuiyang 550004China Department of Orthopaedics The First People's Hospital of GuiyangGuiyang 550004China center for Tissue Engineering and stem cell Research Guizhou Medical UniversityGuiyang 550004China Department of Otolaryngology Head and Neck surgery Xinqiao HospitalArmy Medical University(Third Military Medical University)Chongqing 400037China Department of Dental implant Stomatological Hospital of GuiyangGuiyang 550000China

This study presents the development and evaluation of a poly(3-hydroxybutyrate-co-4-hydroxybutyrate)(P34HB)ultrafine fiber slow-release system for in vivo osteogenic induction of human umbilical cord mesenchymal stem cells(HUc.s.s).Utilizing dual-nozzle and cell electrospinning techniques,the system encapsulates L-ascorbic acid-2-phosphate magnesium(AsP),βB-glycerophosphate sodium and dexamethasone(DEX)within the'fbers,ensuring sustained osteogenic *** scaffold's morphology,characterization,hydrophilicity,mechanical properties and cellular behavior were *** subcutaneous implantation in rabbits was con-ducted to observe its ectopic osteogenic induction *** fabricated P34HB ultrafine fiber slow-release *** ization *** distribution of HUc.s.s and inducing components within the scaffold,with no chemical reactions affecting the active *** vitro tests showc.s.d a prolonged release of DEX and AsP,while biocompatibility assays highlighted the scaffold's suitability for cellular *** Red,type I collagen,and osteopontin(OPN)staining verifed the scaffold's potent osteogenic induction effect on ***,immediate implantation into New Zealand White rabbits led to significant new bone formation within 8 *** findings underscore the system's potential for immediate in vivo implantation without prior in vitro induction,marking a promising advancement in bone tissue engineering.

关键词： bone tissue engineering cell electrospinning in vivo induction immediate implantation biomaterials

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Facile synthesis of Polyisothioureas via Alternating copolymerization of Aziridines and Isothiocayanates

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chinese Journal of Polymer science 2024年第3期42卷 292-298,I0005页

作者： Hao-Tian Zhang Ming-Xin Niu Qi Zhang chen-yang Hu Xuan Pang Key Laboratory of Polymer Ecomaterials Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022China Department of Materials science and Engineering Jilin UniversityChangchun 130025China school of Applied chemistry and Engineering University of Science and Technology of ChinaHefei 230026China

Isothiourea is an important class of sulfur-containing molecules showing unique catalytic and biological activities. As such,polyisothiourea is envisioned to be an interesting type of polymer that potentially exhibits a number of interesting properties. However, there is no acc.s. to synthesizing well-defined polyisothiourea, and currently isothiourea-containing polymers are mainly prepared by immobilizing onto other polymer's side chain. Herein, we report the first facile synthesis of polyisothioureas via alternating copolymerization of aziridines and isothiocayanates. Mediated by the catalytic.s.stem of phosphazene superbases/alcohol, a broad scope of aziridines and isothiocayanates could be transformed into polyisothioureas with adjustable substitutions(11 examples). The structures of obtained polyisothioureas were fully characterized with ^(1)H-NMR, ^(13)c-NMR, and ^(1)H-^(13)c HMBc NMR. Moreover, the polyisothioureas show tunable thermal properties depending on substitutions on the isothiourea linkages. The novel structure of these polyisothioureas will enable a powerful platform for the discovery of nextgeneration functional plastics.

关键词： Polyisothioureas Alternative copolymer Aziridines Isothiocayanates

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Unravelling cOVID-19 waves in Rio de Janeiro city:Qualitative insights from nonlinear dynamic analysis

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Infectious Disease Modelling 2024年第2期9卷 314-328页

作者： Adriane s.Reis Laurita dos santos Américo cunha Jr Thaís c.R.O.Konstantyner Elbert E.N.Macao Institute of science and Technology Federal University of São PauloSão Josédos CamposSPBrazil Physics Institute University of São PauloSão PauloSPBrazil scientific and Technological Institute Universidade BrasilSão PauloSPBrazil Department of Applied Mathematics Rio de Janeiro State UniversityRio de JaneiroRJBrazil Paulista school of Medicine Federal University of São PauloSão PauloSPBrazil

since the cOVID-19 pandemic was first reported in 2019,it has rapidly spread around the *** countries implemented several measures to try to control the virus *** healthcare system and consequently the general quality of life population in the cities have all been significantly impacted by the coronavirus *** different waves of contagious were responsible for the increase in the number of c.s.s that,unfortunately,many times lead to *** this paper,we aim to characterize the dynamics of the six waves of c.s.s and deaths caused by cOVID-19 in Rio de Janeiro city using techniques such as the Poincaréplot,approximate entropy,second-order difference plot,and central tendency *** results reveal that by examining the structure and patterns of the time series,using a set of non-linear techniques we can gain a better understanding of the role of multiple waves of cOVID-19,also,we can identify underlying dynamics of disease spreading and extract meaningful information about the dynamical behavior of epidemiological time *** findings can help to closely approximate the dynamics of virus spread and obtain a correlation between the different stages of the disease,allowing us to identify and categorize the stages due to different virus variants that are reflected in the time series.

关键词： cOVID-19 Nonlinear analysis Poincaréplot central tendency measure

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TWAs facilitates gene-scale trait genetic dissection through gene expression,structural variations,and alternative splicing in soybean

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Plant communications 2024年第10期5卷 16-32页

作者： Delin Li Qi Wang Yu Tian Xiangguang Lyv Hao Zhang Huilong Hong Huawei Gao Yan-Fei Li chaosen Zhao Jiajun Wang Ruizhen Wang Jinliang yang Bin Liu Patrick s.schnable James c.s.hnable Ying-Hui Li Li-Juan Qiu The National Key Facility for crop Gene Resourc.s.and Genetic Improvement(NFcRI)/Key Laboratory of crop Gene Resource and Germplasm Enhancement(MOA)/state Key Laboratory of crop Gene Resourc.s.and Breeding Institute of Crop ScienceChinese Academy of Agricultural SciencesBeijing 100081China college of Agriculture Northeast Agricultural UniversityHarbin 150030China National Nanfan Research Institute(sanya) Chinese Academy of Agricultural SciencesSanya 572024China crops Research Institute of Jiangxi Academy of Agricultural sciences Nanchang 330200China soybean Research Institute Heilongjiang Academy of Agricultural SciencesHarbin 150086China Department of Agronomy and Horticulture University of Nebraska-LincolnLincolnNE 68583USA Department of Agronomy Iowa State UniversityAmesIA 50011USA

A genome-wide association study(GWAs)identifies trait-associated loci,but identifying the causal genes can be a bottleneck,due in part to slow decay of linkage disequilibrium(LD).A transcriptome-wide association study(TWAs)addresses this issue by identifying gene expression-phenotype associations or integrating gene expression quantitative trait loci with GWAs ***,we used self-pollinated soybean(Glycine max[L.]Merr.)as a model to evaluate the application of TWAs to the genetic dissection of traits in plant species with slow LD *** generated RNA sequencing data for a soybean diversity panel and identified the genetic expression regulation of 29286 soybean *** TWAs solutions were less affected by LD and were robust to the source of expression,identifing known genes related to traits from different tissues and developmental *** novel pod-color gene L2 was identified via TWAs and functionally validated by genome *** introducing a new exon proportion feature,we significantly improved the detection of expression variations that resulted from structural variations and alternative *** a result,the genes identified through our TWAs approach exhibited a diverse range of causal variations,including sNPs,insertions or deletions,gene fusion,copy number variations,and alternative *** this approach,we identified genes associated with flowering time,including both previously known genes and novel genes that had not previously been linked to this trait,providing insights complementary to those from *** summary,this study supports the application of TWAs for candidate gene identification in species with low rates of LD decay.

关键词： eQTLs TWAs structural variation alternative splicing soybean

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Effec.s.of dietary clostridium butyricum and rumen protected fat on meat quality,oxidative stability,and chemical composition of finishing goats

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Journal of Animal science and Biotechnology 2024年第2期15卷 911-924页

作者： Meimei Zhang Zhiyue Zhang Xinlong Zhang changming Lu Wenzhu yang Xiaolai Xie Hangshu Xin Xiaotan Lu Mingbo Ni Xinyue yang Xiaoyang Lv Peixin Jiao college of Animal science and Technology Northeast Agricultural UniversityHarbin 150030People’s Republic of China Lethbridge Research and Development centre Lethbridge T1J 4B1Canada school of Agriculture and Food sciences The University of QueenslandGattonQLD 4343Australia International Joint Research Laboratory in Universities of Jiangsu Province of china for Domestic Animal Germplasm Resourc.s.and Genetic Improvement Yangzhou 225009China

Background clostridium butyricum(cB)is a probiotic that can regulate intestinal microbial composition and improve meat *** protected fat(RPF)has been shown to increase the dietary energy density and provide essential fatty ***,it is still unknown whether dietary supplementation with cB and RPF exerts beneficial effec.s.on growth performance and nutritional value of goat *** study aimed to investigate the effec.s.of dietary cB and RPF supplementation on growth performance,meat quality,oxidative stability,and meat nutritional value of finishing ***-two goats(initial body weight,20.5±0.82 kg)were used in a completely randomized block design with a 2 RPF supplementation(0 vs.30 g/d)×2 cB supplementation(0 vs.1.0 g/d)factorial treatment *** experiment included a 14-d adaptation and 70-d data and sample collection *** goats were fed a diet consisted of 400 g/kg peanut seedling and 600 g/kg corn-based concentrate(dry matter basis).Result Interaction between cB and RPF was rarely observed on the variables measured,except that shear force was reduced(PcB or RPF alone or their combination;the increased intramuscular fat(IMF)content with adding RPF was more pronounced(PcB than without cB *** pH24h(P=0.009),a*values(P=0.007),total antioxidant capacity(P=0.050),glutathione peroxidase activities(P=0.006),concentrations of 18:3(Psaturated fatty acids(P=0.048)were increased,whereas the L*values(Pshear force(P=0.050)and malondialdehyde content(P=0.044)were decreased by adding ***,cB supplementation increased essential amino acid(P=0.027),flavor amino acid(P=0.010)and total amino acid contents(P=0.024)as well as upregulated the expression of lipoprotein lipase(P=0.034)and peroxisome proliferator-activated receptorγ(PPARγ)(P=0.012),and downregulated the expression of stearoyl-coA desaturase(scD)(P=0.034).The RPF supplementation increased dry

关键词： chemical composition clostridium butyricum Goats Meat quality Oxidative stability Rumen protected fat

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