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Stream-dwelling fungal decomposer communities along a gradient of eutrophication unraveled by 454 pyrosequencing

Fungal Diversity 2015年第1期70卷 127-148页

作者： S.Duarte F.Bärlocher J.Trabulo F.cássio c.pascoal centre of Molecular and Environmental Biology(cBMA) Department of BiologyUniversity of MinhoCampus de Gualtar4710-057 BragaPortugal Department of Biology Mount Allison UniversitySackvilleNB E4L 1G7Canada

Microbial decomposers,especially a fungal group called aquatic hyphomycetes,play a critical role in processing plant litter in freshwaters by increasing its palatability to invertebrate ***,communities of aquatic hyphomycetes have been assessed through the identification of spores,which misses non-sporulating *** new technologies,454 pyrosequencing stands out as most promising for large-scale species ***,very few attempts have been made to validate its effectiveness for assessing the diversity of stream-dwelling fungal *** attempted to gain greater insight into the diversity of aquatic fungal communities in streams exposed to various degrees of eutrophication by using the 454 pyrosequencing technology.A total of 173,889 ITS2 pyrosequencing reads with hits for fungi were obtained from the 5 investigated *** majority of operational taxonomic units(OTUs)belonged to Ascomycota and the identification to the genus level was achieved for 169 *** the total,135,257 reads(ca.78%)showed close affinities to aquatic hyphomycete *** showed declining fungal diversity in the most eutrophic streams,which was congruent with a reduced diversity found through spore *** patterns revealed by connecting representative OTUs to ITS sequences from aquatic hyphomycetes were similar to those determined by traditional spore identification ***,454 pyrosequencing provided a more comprehensive view of fungal diversity;it captured almost twice as many taxa as spore *** study vali-dates the effectiveness of 454 pyrosequencing for surveying the diversity of stream-dwelling fungal decomposer *** application may accelerate the use of these communities for monitoring the integrity of freshwaters.

关键词： Freshwater ecosystems Plant litter decomposition Aquatic fungi ITS region

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AN AUTOMATED REFERENcE POINT-LIKE APPROAcH FOR MULTIcRITERIA SHORTEST PATH PROBLEMS

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Journal of Systems Science and Systems Engineering 2006年第3期15卷 314-329页

作者： Joo c. N.cLíMAcO José M.F.cRAVEIRINHA Marta M.B.pascoal Faculdade de Economia da Universidade de coimbra Avenida Dias da Silva 165 3004-512 CoimbraPortugal.Instituto de Engenharia de Sistemas e Computadores-CoimbraRua Antero de Quental 199 3000-033 Coimbra Portugal Instituto de Engenharia de Sistemas e computadores-coimbra Rua Antero de Quental 199 3000-033 Coimbra Portugal.Departamento de Engenharia Electrotécnica e ComputadoresPolo II da Universidade de Coimbra Pinhal de Marrocos3030-290 CoimbraPortugal Instituto de Engenharia de Sistemas e computadores-coimbra Rua Antero de Quental 1993000-033 CoimbraPortugal.Departamento de MatemáticaPolo I da Universidade de CoimbraApartado 30083001-454 CoimbraPortugal

In this paper we introduce a method of analysis for the automated ordenng and selection or solutions of a multicriteria shortest path model. The method is based on a reference point approach, where the paths in a specific priority region are ranked by non-decreasing order of a chebyshev metric. In order to list paths according with this objective function a labelling algorithm is proposed. The developed method is applied in a video-traffic routing context. computational results are presented and analysed, for randomly generated networks of significant dimension.

关键词： Routing automated decision multicriteria reference point

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血浆和(或)酵母提取物对7-63日龄仔猪生长性能和肠道形态的影响

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饲料研究 2014年第9期37卷 21-27页

作者： Leandro césar Milagres Rigueira Maria cristina Thomaz Dalton césar Milagres Rigueira Leonardo Augusto Fonseca pascoal Alessandro Borges Amorim Fábio Enrique Lemos Budio 周小辉骆明霞严书林

研究旨在评估在7～63日龄仔猪日粮中添加血浆和（或）酵母提取物对仔猪日增质量、日采食量和饲料转化率及肠道形态的影响。试验采用288头体质量为（2．57±0．05）kg的7日龄仔猪，按照日粮差别随机分为4组，每组6个重复，每重复12头仔... 详细信息

研究旨在评估在7～63日龄仔猪日粮中添加血浆和（或）酵母提取物对仔猪日增质量、日采食量和饲料转化率及肠道形态的影响。试验采用288头体质量为（2．57±0．05）kg的7日龄仔猪，按照日粮差别随机分为4组，每组6个重复，每重复12头仔猪。试验预备期I（7～21日龄）、预备期II（22—35日龄）及第1阶段（36～49日龄）各组日粮为对照组（cD组），不添加血浆或酵母提取物；血浆组（PL组），添加6％、4％和2％血浆；酵母提取物组（YE组），添加6％、4％和2％酵母提取物；血浆＋酵母提取物组（PL＋YE组），血浆和酵母提取物分别添加3％、2％和1％。第1I阶段（50—63日龄）所有仔猪饲喂相同的饲料。7～21和7～28日龄没有观察到生长性能的差别，但是第7天一第35天PL＋YE组的日增质量有优势（cD、PL、YE和PL＋YE组的日增质量分别为268、278、271和288g）。观察7～49及7～63日龄发现，PL＋YE组仔猪的日增质量（分别为330和519g）和日采食量（分别为307和647g）都比cD组的日增质量（分别为295和486g）和日采食量（分别为266和594g）要高。不同组间的肠道形态没有显著差异（P〉0．05）。试验结果表明，血浆和酵母提取物提高7～63日龄的仔猪日增质量，但是没有影响到仔猪的肠道形态。

关键词：饲料转化率采食量仔猪肠绒毛增质量

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Prevalence and characterization of the cerebral Palsy in Maceio,a Northeast city of Brazil

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World Journal of Neuroscience 2018年第3期8卷 333-341页

作者： Marcia Andreya Zanon Amanda Kelly Martin Araujo Edson V.c.Neto Larissa c.Mendes David Balbino pascoal Euclides Mauricio Trindade Filho Reidson Beiriz Vercosa Lysien Ivania Zambrano Janise Dal Pai Jose claudio da Silva Faculdade Estacio de Sa de Alagoas MaceioBrasil Universidade Estadual de ciencias da Saude de Alagoas-UNcISAL MaceioBrasil centro Universitario cESMAc MaceioBrasil centro Universitario UNIT MaceioBrasil Facultad de ciencias Medicas Universidad Nacional Autonoma de HondurasTegucigalpaHonduras Universidade Federal de Sao Paulo-UNIFESP Sao PauloBrasil Faculdade UNIRB de Arapiraca ArapiracaBrasil

Background: cerebral palsy is a group of disorders arising from a static damage or brain development defects occurring during fetal life and in the first months of life. Methods: The sample consisted of 800 individuals living in 50 districts of the city of Maceio. A standardized questionnaire was applied. Results: The prevalence of cerebral palsy in the sample was 5/1000. All were born at term, 75% were male, 50% had severe cerebral palsy and 50% was moderate. 75% had quadriplegia and 25% had diplegia. Fifty percent of the cerebral palsy was caused by meningitis and 50% for prolonged labor. conclusions: The prevalence of cerebral palsy in Maceió is 140.38% higher than the highest prevalence found in developed countries, predominantly in low-income and related to postnatal infection in families.

关键词： cerebral Palsy Epidemiology Motor Disorders Muscle Weakness Muscle Tonus

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