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Whole‑genome transcriptome and DNA methylation dynamics of pre‑implantation embryos reveal progression of embryonic genome activation in buffaloes

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Journal of Animal Science and Biotechnology 2023年第6期14卷 2411-2427页

作者： Penghui Fu Du Zhang chunyan Yang Xiang yuan Xier Luo Haiying Zheng Yanfei Deng Qingyou Liu kuiqing cui Fei Gao Deshun Shi State key Laboratory for conservation and Utilization of Subtropical Agro‑Bioresources&Guangxi key Laboratory of Animal Breeding and Disease control Guangxi UniversityNanning 530004China. college of Animal Science and Technology Southwest UniversityChongqing 402460China. Genome Analysis Laboratory of the Ministry of Agriculture Agricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120China. Guangxi key Laboratory of Buffalo Genetics Reproduction and BreedingGuangxi Buffalo Research InstituteChinese Academy of Agricultural ScienceNanning 530001China Guangxi Academy of Medical Sciences and the People’s Hospital of Guangxi Zhuang Autonomous Region Nanning 530016China. Guangdong Provincial key Laboratory of Animal Molecular Design and Precise Breeding School of Life Science and Engineering Foshan UniversityFoshan 528225China. comparative Pediatrics and Nutrition Department of Veterinary and Animal SciencesFaculty of Health and Medical SciencesUniversity of CopenhagenDK 1870 C FrederiksbergDenmark.

Background During mammalian pre-implantation embryonic development(PED),the process of maternal-to-zygote transition(MZT)is well orchestrated by epigenetic modification and gene sequential expression,and it is related to the embryonic genome activation(EGA).During MZT,the embryos are sensitive to the environment and easy to arrest at this stage in ***,the timing and regulation mechanism of EGA in buffaloes remain *** Buffalo pre-implantation embryos were subjected to trace cell based RNA-seq and whole-genome bisulfite sequencing(WGBS)to draw landscapes of transcription and *** typical developmental steps were classified during buffalo *** major EGA was identified at the 16-cell stage by the comprehensive analy-sis of gene expression and DNA methylation *** weighted gene co-expression network analysis,stage-spe-cific modules were identified during buffalo maternal-to-zygotic transition,and key signaling pathways and biological process events were further *** and continuous activation of these pathways was necessary for success of buffalo *** addition,the hub gene,cDk1,was identified to play a critical role in buffalo *** Our study provides a landscape of transcription and DNA methylation in buffalo PED and reveals deeply the molecular mechanism of the buffalo EGA and genetic programming during buffalo *** will lay a foundation for improving the in vitro development of buffalo embryos.

关键词： Buffalo DNA methylome Embryonic genome activation Maternal-to-zygote transition Transcriptome

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Genome Size of Alexandrium catenella and Gracilariopsis lemaneiformis Estimated by Flow cytometry

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Journal of Ocean University of china 2016年第4期15卷 704-710页

作者： DU Qingwei SUI Zhenghong cHANG Lianpeng WEI Huihui LIU yuan MI Ping SHANG Erlei Zeeshan Niaz QUE Zhou key Laboratory of Marine Genetics and Breeding of Ministry of Education of china College of Marine Life Sciences Ocean University of China Department of Microbiology Hazara University MansehraKPK

Flow cytometry(FcM) technique has been widely applied to estimating the genome size of various higher plants. However, there is few report about its application in algae. In this study, an optimized procedure of FcM was exploited to estimate the genome size of two eukaryotic algae. For analyzing Alexandrium catenella, an important red tide species, the whole cell instead of isolated nucleus was studied, and chicken erythrocytes were used as an internal reference. The genome size of A. catenella was estimated to be 56.48 ± 4.14 Gb(1c), approximately nineteen times larger than that of human genome. For analyzing Gracilariopsis lemaneiformis, an important economical red alga, the purified nucleus was employed, and Arabidopsis thaliana and chondrus crispus were used as internal references, respectively. The genome size of Gp. lemaneiformis was 97.35 ± 2.58 Mb(1c) and 112.73 ± 14.00 Mb(1c), respectively, depending on the different internal references. The results of this research will promote the related studies on the genomics and evolution of these two species.

关键词： Genome Arabidopsis eukaryotic nucleus genomics references cytometry algae purified chicken

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基于In Silicon模拟消化的北极虾DPP-Ⅳ抑制肽活性分析

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中国食品添加剂 2024年第1期35卷 127-135页

作者：刘浩思徐春明田源韩爱萍刘孝飞李振华北京工商大学轻工科学技术学院北京100048 北京玫瑰日记生物科技有限公司北京100024 北京有肌时代科技有限公司北京100024

北极虾具有很高的营养价值,在食品领域已引起越来越多的关注。对北极虾蛋白进行In Silicon模拟消化获得寡肽,通过PeptideRanker活性评分及理化性质分析,从中筛选出具有潜在生物活性的寡肽。使用ToxinPred分析和BIOPEP-UWM生物活性预测,... 详细信息

北极虾具有很高的营养价值,在食品领域已引起越来越多的关注。对北极虾蛋白进行In Silicon模拟消化获得寡肽,通过PeptideRanker活性评分及理化性质分析,从中筛选出具有潜在生物活性的寡肽。使用ToxinPred分析和BIOPEP-UWM生物活性预测,发现部分寡肽具有二肽基肽酶-Ⅳ(dipeptidyl peptidase-Ⅳ,DPP-Ⅳ)抑制活性,最终确定WFP(一种三肽,Trp-Phe-Pro)具有最优的DPP-Ⅳ抑制活性肽。分子对接表明,WFP和DPP-Ⅳ能够形成稳定的复合物,其结合能为-6.93 kcal/mol,进一步研究表明,WFP通过与DPP-Ⅳ S1、S2、S3三个活性口袋中的9个氨基酸残基发生相互作用而抑制其活性。本研究为阐释北极虾营养价值及生物活性肽的开发提供了理论依据。

关键词： In Silicon 分子对接 DPP-Ⅳ 细胞色素c氧化酶亚基Ⅰ 寡肽

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Inhibition of autophagy significantly enhances combination therapy with sorafenib and HDAc inhibitors for human hepatoma cells

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World Journal of Gastroenterology 2014年第17期20卷 4953-4962页

作者： Hang yuan Ai-Jun Li Sen-Lin Ma Long-Jiu cui Bin Wu Lei Yin Meng-chao Wu Department of the 2~(nd) Special Treatment Eastern Hepatobiliary Surgery HospitalSecond Military Medical University

AIM: To clarify whether histone deacetylase inhibitors histone deacetylase inhibitors (HDAcIs) can sensitize hepatocellular carcinoma (Hcc) cells to sorafenib treatment. METHODS: Bax, Bcl-2, ATG5-ATG12, p21, and p27 protein levels in Hep3B, HepG2, and PLc/PRF/5 cells were examined by Western blot. cck8 and a fluoro-metric caspase-3 assay were used to examine cellular viability and apoptosis levels. The effect of Beclin-1 on sensitization of Hcc cells to sorafenib was examined by transfecting Beclin-1 siRNA into Hep3B, HepG2, and PLc/PRF/5 cells. RESULTS: Autophagy inhibition enhances the inhibitory effects of vorinostat and sorafenib alone or in combination on Hcc cell growth. Vorinostat and sorafenib synergistically induced apoptosis and cell cycle alterations. Western blot data indicated that HDAcIs and Beclin- 1 knockdown increased the p53 acetylation level. The knockdown of Beclin-1 enhanced the synergistic effect of the combination of vorinostat with sorafenib. cONcLUSION: HDAcIs can sensitize Hcc cells to sorafenib treatment by regulating the acetylation level of Beclin-1. (c) 2014 Baishideng Publishing Group co., Limited. All rights reserved.

关键词： Hepatocellular carcinoma Histone deacetylase inhibitors Autophagy Sorafenib chemoresistance

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Enhancing electromagnetic wave absorption in carbon fiber using FeS_(2)nanoparticles

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Nano Research 2023年第7期16卷 9591-9601页

作者： Yuying Guo Meng Zhang Tingting cheng Yuxin Xie Laibin Zhao Liang Jiang Wenxin Zhao Liying yuan Alan Meng Jian Zhang Ting Wang Zhenjiang Li college of Electromechanical Engineering Key Laboratory of Polymer Material Advanced Manufacturing’s Technology of Shandong provinceCollege of Materials Science and EngineeringQingdao University of Science and TechnologyQingdao 266061China college of chemical Engineering Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life ScienceMOEShandong Key Laboratory of Biochemical AnalysisCollege of Chemistry and Molecular EngineeringQingdao University of Science and TechnologyQingdao 266042China college of Textiles and clothing Qingdao UniversityQingdao 266071China

carbon-based electromagnetic wave absorbing materials(absorbers)adhered with metallic sulfide nanoparticles of good electrical conductivity attract increasing researchers’*** this study,on the basis of carbon fiber(c_(f))@Fe_(3)O_(4) nanocomposites obtained by the electrostatic spinning and reflow method,c_(f)@FeS_(2)nanocomposite was successfully prepared during a further hydrothermal *** products exhibit excellent electromagnetic wave absorption performances with a minimum reflection loss(RLmin)of-54.11 dB at 2.13 mm matching *** the same time,the optimal effective absorption bandwidth(EAB)value of 6.04 GHz at a thickness of 1.98 mm covers the whole ku band,suggesting its excellent electromagnetic wave absorption *** addition,the interlaced network structure constructed by carbon fiber,outstanding conductivity of FeS_(2)nanoparticles,and interfacial polarization from hetero-structure play significant parts in enhancing the electromagnetic parameters and absorption *** these results suggest that the c_(f)@FeS_(2)nanocomposites can be taken as a new electromagnetic wave-absorbing material under their low density,simple craft,and strong absorption characteristics.

关键词： constructed network structure carbon fiber(c_(f))@FeS_(2)nanocomposite enhanced dielectric loss microwave attenuation mechanism

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High-entropy alloy catalysts:From bulk to nano toward highly efficient carbon and nitrogen catalysis

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carbon Energy 2022年第5期4卷 731-761页

作者： Lanlan Yu kaizhu Zeng chenghang Li Xiaorong Lin Hanwen Liu Wenhui Shi Hua-Jun Qiu Yifei yuan Yonggang Yao State key Laboratory of Materials Processing and Die and Mould Technology School of Materials Science and EngineeringHuazhong University of Science and TechnologyWuhanHubeiChina college of chemistry and Materials Engineering Wenzhou UniversityWenzhouZhejiangChina Shenzhen R&D center for Al-based Hydrogen Hydrolysis Materials School of Materials Science and EngineeringHarbin Institute of Technology(Shenzhen)ShenzhenGuangdongChina

High-entropy alloys(HEAs)have attracted widespread attention as both structural and functional materials owing to their huge multielement composition space and unique high-entropy mixing ***,emerging HEAs,either in nano or highly porous bulk forms,are developed and utilized for various catalytic and clean energy applications with superior activity and remarkable *** catalysts,HEAs possess some unique advantages,including(1)a multielement composition space for the discovery of new catalysts and fine-tuning of surface adsorption(i.e.,activity and selectivity),(2)diverse active sites derived from the random multielement mixing that are especially suitable for multistep catalysis,and(3)a high-entropy stabilized structure that improves the structural durability in harsh catalytic *** from these inherent advantages,HEA catalysts have demonstrated superior catalytic performances and are promising for complex carbon(c)and nitrogen(N)cycle reactions featuring multistep reaction pathways and many different ***,the design,synthesis,characterization,and understanding of HEA catalysts for c-and N-involved reactions are extremely challenging because of both complex high-entropy materials and complex *** this review,we present the recent development of HEA catalysts,particularly on their innovative and extensive syntheses,advanced(in situ)characterizations,and applications in complex c and N looping reactions,aiming to provide a focused view on how to utilize intrinsically complex catalysts for these important and complex *** the end,remaining challenges and future directions are proposed to guide the development and application of HEA catalysts for highly efficient energy storage and chemical conversion toward carbon neutrality.

关键词： carbon conversion electrocatalysis high-entropy alloy catalysts multifunctionality nitrogen conversion

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Dynamic long-term microstructural and ultrastructural alterations in sensory nerves of rats of paclitaxel-induced neuropathic pain

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chinese Medical Journal 2014年第16期127卷 2945-2952页

作者： Wu yuan Li Jun Zhou Junfei Feng Yi Department of Anesthesia Peking University People's HospitalBeijing 100044 China

Background Paclitaxel,as a first line anti-neoplastic compound,frequently produces long-term pain after tumors have been *** manifestations are varied and *** of the nervous system during the development of the neuropathic pain is ***,eady diagnosis and treatment is often unsatisfying for *** study aimed to promote considerate understanding of the structural alteration of sensory *** All rats were simply randomized into 3 groups：paclitaxel group,vehicle group and saline *** established rat model of paclitaxel-induced peripheral neuropathy （2 mg/kg） was chosen for our research,behavior tests were operated during the procedure of 56 *** rats were sampled on days 0,3,7,28 and *** hind paw plantar skin,sciatic nerves,dorsal root ganglion and attached fibers,and lumbar spinal cord were processed for light and electron *** differences among 3 groups were analyzed with one-way analysis of vadance （ANOVA）.Results We affirmed that paclitaxel-induced mechano-aliodynia and mechano-hyperalgesia occured after a 3-7-day delay,and this pain peaked at day 28 and persisted to day *** and vehicle treatment both evoked ***-induced axonal and myelin sheath degeneration was *** days 3 and 7,significant increases in atypical mitochondria in both myelinated axons and c-fibers of paclitaxel-treated nerves indicated that injured mitochondria correlated to specific paclitaxel-induced neuropathic pain,and the abnormity sustained till day *** was unaffected in myelinated axons or c-fibers in paclitaxel-or vehicle-treated *** increase of G ratio was evident with paclitaxel injection at days 7 and *** Our research suggests a causal role for axonal degeneration,abnormalities in axonal mitochondria,and structural modification of axonal microtubules in paclitaxel-induced neuropathic pain,and the abnormal mitochondria could be connected t

关键词： paclitaxel neuropathic pain ultrastructure microstructure

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Electrical characters and optical emission spectra of VBD coupled SBD excited by sine Ac voltage in atmospheric air

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Plasma Science and Technology 2017年第6期19卷 45-50页

作者： Zilu ZHAO Dezheng YANG Wenchun WANG Hao yuan Li ZHANG Sen WANG key Lab of Materials Modification Dalian University of Technology Ministry of Education Dalian 116024 People＇s Republic of China

In this paper,volume coupled surface barrier discharge（V-SBD） with three structures possessing different volumes is excited by sine Ac power in atmospheric *** images,waveforms of applied voltage and discharge current,and optical emission spectra simulating rotational and vibrational temperatures are recorded and *** effects of applied voltage on emission intensities of N2（c^3Πu→ B^3Πg） and N2^＋（B^2∑u^＋ → X^2Eg^＋）,and rotational and vibrational temperatures are *** results show that as applied voltage rises,emission intensities and rotational temperatures increase while vibrational temperatures *** addition it is found that,as applied voltage varies,the rotational temperature of surface discharge changes faster than that of volume discharge.

关键词： dielectric barrier discharge surface discharge volume discharge optical emission spectra

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A candy-like photocatalyst by wrapping co, N-co-doped hollow carbon sphere with ultrathin mesoporous carbon nitride for boosted photocatalytic hydrogen evolution

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chinese chemical Letters 2023年第2期34卷 367-371页

作者： Jinyuan Liu Shumin Zhu Bin Wang Ruizhe Yang Rong Wang Xingwang Zhu Yanhua Song Junjie yuan Hui Xu Huaming Li School of chemistry and chemical Engineering Institute for Energy ResearchJiangsu UniversityZhenjiang 212013China college of Environmental Science and Engineering Yangzhou UniversityYangzhou 225009China School of Environmental and chemical Engineering Jiangsu University of Science and TechnologyZhenjiang 212003China

Hierarchical carbon material is used as a star cocatalyst in the field of photocatalysis due to its excellent catalytic properties. In this work, mesoporous carbon nitride sheet(McNS) photocatalyst introduced nitrogen-doped hollow carbon spheres assembled with cobalt nanoparticles(co@NHc) is synthesized by electrostatic adsorption. A series of characterizations are analyzed to display the structures, morphologies and optical properties of as-prepared materials. The photocatalytic activity of co@NHc/McNS material is evaluated with hydrogen evolution under visible light irradiation. The results indicate that 5 wt%co@NHc/McNS material reveals higher photocatalytic activity of hydrogen evolution rate of 3675 μmol/g with 4 h reaction time, which is 159 times than that of pure McNS material. The carbon material with excellent charge transport properties can effectively accelerate the charge transfer from ultrathin McNS to cobalt nanoparticles. The goal of improving the photocatalytic performance of co@NHc/McNS material is achieved. As a result, it provides a feasible and promised approach for doping transition metals to enhance photocatalytic activity.

关键词： candy-like photocatalyst Mesoporous carbon nitride Hollow carbon spheres Photocatalytic hydrogen evolution

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Diarylheptanoid-flavanone Hybrids as Multiple-target Antidiabetic Agents from Alpinia katsumadai

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chinese Journal of chemistry 2021年第11期39卷 3051-3063页

作者： Xiao-Feng He Ji-Jun chen Tian-Ze Li Jing Hu Xiao-Yan Huang Xue-Mei Zhang yuan-Qiang Guo chang-An Geng State key Laboratory of Phytochemistry and Plant Resources in West china Kunming Institute of BotanyChinese Academy of SciencesYunnan Key Laboratory of Natural Medicinal ChemistryKunmingYunnan650201 China college of Pharmacy and Tianjin key Laboratory of Molecular Drug Research Nankai UniversityTianjin300071 China University of chinese Academy of Sciences Beijing100049 China

The EtOAc fraction of Alpinia katsumadai seeds showed significant inhibition on glycogen phosphorylase a(GPa)with inhibitory ratios of 97.9%and 64.6%at concentrations of 200 and 100μg/mL,***-guided isolation afforded 15 new diarylheptanoid-flavanone hybrids,katsumadainols B_(1)-B_(15)(1-15),together with eight known ones(16-23).compounds 4-10 and 12-21 exhibited activity against GPa with Ic_(50) values of 10.1-95.4μmol/L;compounds 4,5,16,and 17 displayed inhibitory effects onα-glucosidase with Ic_(50) values of 7.1,12.4,7.2,and 8.3μmol/L,obviously higher than acarbose(Ic_(50),209.1μmol/L);compounds 4-6,14,16-20,22,and 23 were PTP1B/TcPTP selective inhibitors with Ic_(50) values of 40.7-95.8μmol/L;compounds 4,5,16,and 17 showed DPP4 inhibitory effects with inhibitory ratios of 50.0%-54.2%(200μmol/L).Diarylheptanoid-flavanone hybrids(4,5,16,and 17)with a p-hydroxybenzyl at c-6 position represent a promising class of multiple-target antidiabetic agents inhibiting GPa,α-glucosidase,PTP1B,and DPP4.

关键词： Alpinia katsumadai katsumadainols Bx-B_(15) Natural products Structure elucidation Biological activity

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