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A PTEN translational isoform has PTEN-like activity

chinese Journal of cancer Research 2015年第5期27卷 524-532页

作者： Xie Zhang Bowei Yin Fangfang Zhu Guochang huang Hong Li Department of Gastroenterology Ningbo Medical CenterLiHuiLi Hospital School of Medicine Ningbo University Ningbo Medical center Ningbo Medical CenterLiHuiLi hospital Minimally Invasive Abdominal Surgery Ningbo Medical CenterLiHuiLi hospital

Background： To identify PTEN isoform and explore its potential role in tumor suppression. Methods： Western blotting, over-expression, shRNA mediated knocking-down, and bioinformatic analysis were used to identify PTEN isoform and test its effect on PI3K-Akt signaling pathway. cell proliferation, apoptosis, and migration assays were used to test PTEN isoform＇s biological activities. Results： The PTEN isoform is about 15 kDa bigger than PTEN and its expression is dependent on PTEN status. Immunoprecipitation for PTEN isoform followed by screening with antibodies against ISG15, SUMO1/2/3, Ubiquitin, and Nedd8 showed the identified PTEN isoform is not a general proteinaceous post-translational modification. In addition, overexpression of PTEN cDNA in cells did not generate PTEN isoform whereas knocking-down of PTEN reduced the protein levels of both PTEN and PTEN isoform in a proportional manner. Analysis of PTEN DNA sequence disclosed an alternative translational starting code （cTG） upstream of canonical PTEN coding sequence. Expression of cloned PTEN isoform generated a protein with a size about 15 kDa bigger than PTEN and suppressed PI3K-Akt signaling pathway in cells. Overexpression of PTEN isoform also led to decrease in cell growth and enhanced serum starvation--and UV irradiation--induced apoptosis through activation of caspase 3. Finally, expression of PTEN isoform inhibited cell migration in scratch assay. conclusions： PTEN isoform has PTEN-Iike activity and might be a new tumor suppressor.

关键词： PTEN PTEN isoform rumor suppressor PI3K-Akt cell growth apoptosis cell migration

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Quercetin exerts anti-inflammatory effects via inhibiting tumor necrosis factor-α-induced matrix metalloproteinase-9 expression in normal human gastric epithelial cells

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World Journal of Gastroenterology 2022年第11期28卷 1139-1158页

作者： Hsi-Lung Hsieh Ming-chin Yu Li-ching cheng Mei-Yi chu Tzu-Hao huang Ta-Sen Yeh Ming-Ming Tsai Department of Nursing Division of Basic Medical SciencesChang-Gung University of Science and TechnologyTaoyuan 333TaiwanChina Research center for chinese Herbal Medicine College of Human EcologyChang Gung University of Science and TechnologyTaoyuan 333TaiwanChina Department of Neurology Chang Gung Memorial HospitalTaoyuan 333TaiwanChina Department of General Surgery New Taipei Municipal TuCheng HospitalNew Taipei 236TaiwanChina college of Medicine Chang Gung UniversityTaoyuan 333TaiwanChina Department of General Surgery Chang Gung Memorial HospitalTaoyuan 333TaiwanChina

BAcKGROUND Gastric injury is the most common digestive system disease worldwide and involves inflammation,which can lead to gastric ulcer or gastric cancer(Gc).Matrix metallopeptidase-9[MMP-9(gelatinase-B)]plays an important role in inflammation and Gc *** and quercetin-rich diets represent potential food supplements and a source of medications for treating gastric injury given their anti-inflammatory ***,the effects and mechanisms of action of quercetin on human chronic gastritis and whether quercetin can relieve symptoms remain *** To assess whether tumor necrosis factor-α(TNF-α)-induced MMP-9 expression mediates the anti-inflammatory effects of quercetin in normal human gastric mucosal epithelial *** The normal human gastric mucosa epithelial cell line GES-1 was used to establish a normal human gastric epithelial cell model of TNF-α-induced MMP-9 protein overexpression to evaluate the antiinflammatory effects of *** cell counting Kit-8 assay was used to evaluate the effects of varying quercetin doses on cell viability in the normal GES-1 cell *** migration was measured using Transwell *** expression of proto-oncogene tyrosine-protein kinase Src(cSrc),phospho(p)-c-Src,extracellular-signal-regulated kinase 2(ERK2),p-ERK1/2,c-Fos,p-c-Fos,nuclear factor kappa B(NF-κB/p65),and p-p65 and the effects of their inhibitors were examined using Western blot analysis and measurement of luciferase activity.p65 expression was detected by ***-9 m RNA and protein levels were measured by quantitative reverse transcription polymerase chain reaction(q RT–PcR)and gelatin zymography,*** q RT-PcR and gelatin zymography showed that TNF-αinduced MMP-9 m RNA and protein expression in a dose-and time-dependent *** effects were reduced by the pretreatment of GES-1 cells with quercetin or a TNF-αantagonist(TNFR inhibitor)in a dose-and timedependent *** and TNF-αa

关键词： Anti-inflammatory Quercetin Matrix metallopeptidase-9 Tumor necrosis factor-α Normal human gastric epithelial cells

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congenital disorder of glycosylation caused by mutation of ATP6AP1 gene (c.1036G>A) in a chinese infant: A case report

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World Journal of clinical cases 2021年第26期9卷 7876-7885页

作者： Xia Yang Zi-Li Lv Qing Tang Xiu-Qi chen Li huang Mei-Xiong Yang Lian-cheng Lan Qing-Wen Shan Department of Pediatrics The First Affiliated Hospital of Guangxi Medical UniversityNanning 530021Guangxi Zhuang Autonomous RegionChina Department of Pathology The First Affiliated Hospital of Guangxi Medical UniversityNanning 530021Guangxi Zhuang Autonomous RegionChina

BAcKGROUND The ATP6AP1 gene coding for the accessory protein Ac45 of the vacuolar-type adenosine triphosphatases(V-ATPase)is located on chromosome *** in certain subunits or accessory subunits of the V-ATPase can lead to congenital disorders of glycosylation(cDG).cDG is a group of metabolic disorders in which defective protein and lipid glycosylation processes affect multiple tissues and ***,the clinical presentation of patients with ATP6AP1-cDG varies *** this report,we present a case of ATP6AP1-cDG in a chinese infant,with clinical features and *** SUMMARY An 8-mo-old boy was admitted to our hospital because unexplained hepatosplenomegaly and elevated transaminases that had been noted while he was being treated for a cough at a local hospital.A post-admission examination at our hospital revealed abnormalities in the infant’s liver,brain,and immune ***-based whole exome gene analysis identified a hemizygous pathogenic mutation c.1036G>A(p.E346K)in exon 9 of the ATP6AP1 *** variant of the ATP6AP1 gene has not been reported in East Asian countries until *** Based on the infant’s clinical manifestations and the results of genetic detection,he was clearly diagnosed with *** clinical manifestations of children with cDG vary *** testing analysis helps in the clinical diagnosis of children with cDG.

关键词： congenital disorders of glycosylation ATP6AP1 mutation Hepatopathy Immunodeficiency cognitive impairment case report

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Preparation of dense Ta-LLZO/MgO composite Li-ion solid electrolyte:Sintering, microstructure, performance and the role of MgO

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Journal of Energy chemistry 2019年第12期28卷 8-16页

作者： Xiao huang Yang Lu Zhen Song Tongping Xiu Michael EBadding Zhaoyin Wen cAS Key Laboratory of Materials for Energy conversion Shanghai Institute of CeramicsChinese Academy of ScienceShanghai 200050China corning Incorporated ComingNY 14831USA corning Research center china 200 Jinsu RoadShanghai 201206China University of chinese Academy of Sciences 19A Yuquan RdShijingshan DistrictBeijing 100049China

cubic phase Li7La3Zr2O12(LLZO),a member of the Li–Garnet family,is a promising solid electrolyte and has been widely studied in recent ***,LLZO samples prepared via conventional ambient air sintering reported in the published literature often contain large grains with lower than desired(contact method of co-firing with mother powder method is proposed to prepare high-quality Ta-doped LLZO–MgO composite *** sintering at 1150℃for 5 h,the ceramics can reach relative density of 98.2%,conductivity of 5.17×10^-4 S cm^-1 at 25℃and fracture strength of 150 *** sintered samples have uniform fine-grained microstructure and high critical current densities of 0.75–0.95 mA cm-2 at room temperature in Li–Li symmetry cell with Au *** addition,systematic sintering experiments and characterizations are conducted to explore the function of MgO in inhibiting the Ta-LLZO grain growth and its existing form inside the composite ceramics.

关键词： Li-Garnet Solid electrolyte Mechanism critical current density Sintering

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Mechanism investigation of High-Performance Li-Polysulfide Batteries Enabled by Tungsten Disulfide Nanopetals

Mechanism investigation of High-Performance Li-Polysulfide B...

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中国化学会第三届中国能源材料化学研讨会

作者： huang Shaozhuan Yang Hui Ying Pillar of Engineering Product Development Singapore University of Technology and Design

Understanding the reaction kinetics and mechanism of Li-polysulfide batteries is critical in designing advanced host materials for improved performance. However,up to now,the reaction mechanism within the Li-polysulfide batteries is still ***,we study the reaction mechanism of a high-performance Li-polysulfide battery by in situ X-ray diffraction(XRD) and density functional theory(DFT)

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catalytic carbon dioxide hydrogenation to methane: A review of recent studies

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Journal of Energy chemistry 2016年第4期25卷 553-565页

作者： Xiong Su Jinghua Xu Binglian Liang Hongmin Duan Baolin Hou Yanqiang huang State Key Laboratory of catalysis Dalian Institute of Chemical PhysicsChinese Academy of Sciences Dalian Institute of Standardization

Recently, various efforts have been put forward on the development of technologies for the synthesis of methane from cO2 and H-2, since it can offer a solution for renewable H-2 storage and transportation. In parallel, this reaction is considered to be a critical step in reclaiming oxygen within a closed cycle. Over the years, extensive fundamental research works on cO2 methanation have been investigated and reported in the literatures. In this updated review, we present a comprehensive overview of recent publications during the last 3 years. Various aspects on this reaction system are described in detail, such as thermodynamic considerations, catalyst innovations, the influence of reaction conditions, overall catalytic performance, and reaction mechanism. Finally, the future development of cO2 methanation is discussed. (c) 2016 Science Press and Dalian Institute of chemical Physics, chinese Academy of Sciences. Published by Elsevier B.V. and Science Press. All rights reserved.

关键词： Methanation carbon dioxide Hydrogenation Ni catalyst Ru catalyst

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New Historical Opportunities for the Development of cultural Industries in Guangdong and Macao-Impact of the RcEP Agreement

New Historical Opportunities for the Development of Cultural...

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2022城市群新兴共建——第五届粤港澳大湾区研究生论坛

作者： Yang PENG Xing-yan huang School of Business Macau University of Science and Technology

"Building a base of exchange and cooperation with chinese culture as the mainstream and multiple cultures co-existing" is the development goal for Macau proposed by the central Government in the Outline of the Development Plan of Guangdong-Hong Kong-Macao Greater Bay Area promulgated in 2019, which is of great practical significance in addressing Macau ’ s economic and financial dependence on the gaming industry. After eight years of negotiations, china formally signed the RcEP agreement on November 15, 2020, and it came into effect on January 1, 2022. The RcEP agreement profoundly affects the trade development of participating countries in three areas: tariff concessions, trade in services, and trade rules. Under the influence of the cOVID-19, the cultural exhibition industry has seen a cold winter. However, the cultural exhibition industry based on Guangdong-Macau cooperation has ushered in a new historical development opportunity under the framework of RcEP, which is also more conducive to Macau, giving full play to its geographical advantages and the transformation of its economic structure.

关键词： RcEP Guangdong-Macau cooperation cultural Industry

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Prior transfusion of umbilical cord mesenchymal stem cells can effectively alleviate symptoms of motion sickness in mice through interleukin 10 secretion

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World Journal of Stem cells 2021年第2期13卷 177-192页

作者： Hua-Su Zhu Dong Li cong Li Jin-Xian huang Shan-Shan chen Lan-Bo Li Qing Shi Xiu-Li Ju Department of Pediatrics Qilu HospitalCheeloo College of MedicineShandong UniversityJinan 250012Shandong ProvinceChina Stem cell and Regenerative Medicine Research center Qilu HospitalCheeloo College of MedicineShandong UniversityJinan 250012Shandong ProvinceChina Department of Animal Laboratory Qilu HospitalCheeloo College of MedicineShandong UniversityJinan 250012Shandong ProvinceChina Department of Pediatrics Qilu Hospital of Shandong UniversityJinan 250012Shandong ProvinceChina

BAcKGROUND Motion sickness(MS)is a disease that occurs during unbalanced movement,characterized by gastrointestinal symptoms and autonomic nervous system *** clinical treatments for MS are *** evidence indicates that the levels of pro-inflammatory cytokines increase during MS and are associated with an inner ear immune *** the present study,mesenchymal stem cells(MScs)have been shown to exert strong immunosuppressive *** To explore whether umbilical cord-derived mesenchymal stem cells(Uc-MScs)can prevent the occurrence of MS,and the underlying mechanism regulated by MScs in a mouse model of *** A total of 144(equal numbers of males and females)5wkold BALB/c mice were randomly divided into five groups:Normal group(n=16),MS group(n=32),MScs group(n=32),MS+MScs group(n=32),and MS+AS101/MScs group(n=32).The MScs group(n=32),MS+MScs group(n=32),and MS+AS101/MScs group(n=32)were preventively transplanted with Uc-MScs or AS101-treated Uc-MScs(1×106 cells/mouse).Mice in the MS(n=32),MS+MScs,and MS+AS101/MScs groups were subjected to rotation on a centrifuge for 10 min at 8×g/min for MS model establishment on days 3,5,8,and 10 after Uc-MScs *** Morris water maze(MWM)test was used to observe the symptom of ***-linked immunosorbent assay(ELISA)and reverse transcription-quantitative polymerase chain reaction(RT-qPcR)were used to detect the levels of inflammatory cytokines in mice peripheral blood and the petrous part of the temporal bone *** blot analysis was performed to analyze the JAK2/STAT3 signaling pathway in the cochlear *** examination was performed by hematoxylin and eosin(HE)staining for conventional morphological evaluation in the petrous part of temporal bone *** The MWM test demonstrated that Uc-MScs improved the symptoms of *** MS+MScs group was faster than the MS group on days 3 and 5(P=0.036 and P=0.002,respectively).ELISA and RT-qPcR showed that

关键词： Mesenchymal stem cell Motion sickness Inflammation Immune microenvironment Interleukin-10 JAK2/STAT3

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以高通量方法筛选红桑叶的抗菌活性并进行活性组分的分离研究(英文)

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时珍国医国药 2007年第8期18卷 2065-2068页

作者：周英黄赤夫贵州大学生命科学学院贵州贵阳550025 美国肯塔基大学口腔学院美国40503

目的通过一种简单快速的高通量方法筛选具有抗菌活性的植物提取物,并通过此种方法从有活性的粗提物中纯化活性组分。方法首先建立植物提取物库和抗菌的高通量筛选方法。然后针对转糖链球菌Streptococcus mutans和伴放线放线杆菌Actinoba... 详细信息

目的通过一种简单快速的高通量方法筛选具有抗菌活性的植物提取物,并通过此种方法从有活性的粗提物中纯化活性组分。方法首先建立植物提取物库和抗菌的高通量筛选方法。然后针对转糖链球菌Streptococcus mutans和伴放线放线杆菌Actinobacillus actinomycetemcomitans进行高通量筛选。结果通过筛选发现红桑叶具有很强的抑菌活性。为了进一步研究其活性,将其粗提物通过HPLc进行分离得到71个组分,再通过同样的高通量筛选方法进行抗菌活性测定,对有活性的组分通过血琼脂法进行验证。实验数据表明红桑叶的一个组分#31对两种细菌有几乎完全的抑制(抑制率>99%),而另外两个组分对伴放线放线杆菌Actinobacillus actinomycetemcomitans有部分抑制。结论所建立的高通量筛选方法简便快捷,筛选结果稳定可靠,可用于大规模的植物提取物的抗菌活性筛选。红桑叶具有很强的抗菌活性,且可分离得到活性组分,红桑叶具有进一步开发为抗菌药物和保健品的潜力。

关键词：红桑叶高通量抗菌筛选抗菌活性抑制率

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Loss of LBP triggers lipid metabolic disorder through H3K27 acetylation-mediated c/EBPβ-ScD activation in non-alcoholic fatty liver disease

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Zoological Research 2024年第1期45卷 79-94页

作者： Ya-Ling Zhu Lei-Lei Meng Jin-Hu Ma Xin Yuan Shu-Wen chen Xin-Rui Yi Xin-Yu Li Yi Wang Yun-Shu Tang Min Xue Mei-Zi Zhu Jin Peng Xue-Jin Lu Jian-Zhen huang Zi-chen Song chong Wu Ke-Zhong Zheng Qing-Qing Dai Fan huang Hao-Shu Fang Department of Pathophysiology Anhui Medical UniversityHefeiAnhui 230032China Laboratory Animal Research center School of Basic Medical SciencesAnhui Medical UniversityHefeiAnhui 230032China Department of Hepatobiliary Surgery First Affiliated Hospital of Anhui Medical UniversityHefeiAnhui 230022China college of Animal Science and Technology Jiangxi Agricultural UniversityNanchangJiangxi 330045China

Non-alcoholic fatty liver disease(NAFLD)is associated with mutations in lipopolysaccharide-binding protein(LBP),but the underlying epigenetic mechanisms remain ***,LBP^(-/-)rats with NAFLD were established and used to conduct integrative targetingactive enhancer histone H3 lysine 27 acetylation(H3K27ac)chromatin immunoprecipitation coupled with high-throughput and transcriptomic sequencing analysis to explore the potential epigenetic pathomechanisms of active enhancers of NAFLD exacerbation upon LBP ***,LBP^(-/-)reduced the inflammatory response but markedly aggravated high-fat diet(HFD)-induced NAFLD in rats,with pronounced alterations in the histone acetylome and regulatory *** total,1128 differential enhancer-target genes significantly enriched in cholesterol and fatty acid metabolism were identified between wild-type(WT)and LBP^(-/-)NAFLD *** on integrative analysis,ccAAT/enhancer-binding proteinβ(c/EBPβ)was identified as a pivotal transcription factor(TF)and contributor to dysregulated histone acetylome H3K27ac,and the lipid metabolism gene ScD was identified as a downstream effector exacerbating *** study not only broadens our understanding of the essential role of LBP in the pathogenesis of NAFLD from an epigenetics perspective but also identifies key TF c/EBPβand functional gene ScD as potential regulators and therapeutic targets.

关键词： Non-alcoholic fatty liver disease c/EBPβ Lipopolysaccharide-binding protein H3K27ac Integrative analysis Enhancer

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