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Immunoregulatory and Anti-cancer Activities of combination Treatment of Novel Four-Herb Formula and Doxorubicin in 4T1-Breast cancer Bearing Mice

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chinese Journal of Integrative Medicine 2024年第4期30卷 311-321页

作者： Lea Ling-Yu Kan Ben chung-Lap chan grace gar-Lee Yue LI Pei-ting Sharon Sze-Man Hon HUANg Dan-qi Miranda Sin-Man Tsang clara Bik-San Lau Ping-chung Leung chun-Kwok Wong Institute of chinese Medicine The Chinese University of Hong KongHong Kong SARChina State Key Laboratory of Research on Bioactivities and clinical Applications of Medicinal Plants The Chinese University of Hong KongHong KongChina Department of chemical Pathology The Chinese University of Hong KongHong Kong SARChina china-Australia International Research centre for chinese Medicine School of Health and Biomedical SciencesSTEM CollegeRMIT UniversityBundooraVictoriaAustralia Li Dak Sum Yip Yio chin R&D centre for chinese Medicine The Chinese University of Hong Kong SARHong KongChina

Objective To investigate the in vivo immunomodulatory and anti-tumor mechanisms of the combined treatment of novel Four-Herb formula(4HF)and doxorubicin in triple-negative breast cancer(TNBc).Methods Murine-derived triple-negative mammary carcinoma cell line,4T1 cells,was cultured and inoculated into mouse mammary ***-six mice were randomly assigned into 6 groups(n=11 in ench):naive,control,LD 4HF(low dose 4HF),HD 4HF(high dose 4HF),LD 4HF+D(low dose and doxorubicin),and D(doxorubicin).Apart from the naive group,each mouse received subcutaneous inoculation with 5×10^(5)4T1 cells resuspended in 100µL of normal saline in the mammary fat *** from the day of tumor cell inoculation,tumors were grown for 6 *** LD and HD groups received daily oral gavage of 658 and 2,630 mg/kg 4HF,*** LD 4HF+D group received daily oral gavage of 658 mg/kg 4HF and weekly intraperitoneal injection of doxorubicin(5 mg/kg).The D group received weekly intraperitoneal injections of doxorubicin(5 mg/kg).The treatment naive mice received daily oral gavage of 0.2 mL double distilled water and 0.1 mL normal saline via intraperitoneal injection once a *** control group received daily oral gavage of 0.2 mL double-distilled *** treatment period was 30 *** the end of treatment,mice organs were harvested to analyze immunological activities via immunophenotyping,gene and multiplex analysis,histological staining,and gut microbiota *** Mice treated with the combination of 4HF and doxorubicin resulted in significantly reduced tumor and spleen burdens(Pcyte landscape,and manipulated gut microbiota to favor the anti-tumor immunological ***,immunosuppressive genes,cytokines,and chemokines such as c-c motif chemokine 2 and interleukin-10 of tumors were significantly downregulated(Pcin combination treatment demonstrated synergetic activities and was most effective

关键词： adjunctivetherapy breast cancer cytokines chinese medicine tumor microenvironment

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Autoimmune hepatitis and primary sclerosing cholangitis after direct-acting antiviral treatment for hepatitis c virus:A case report

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World Journal of Hepatology 2024年第2期16卷 286-293页

作者： Yoshiki Morihisa Hobyung chung Shuichiro Towatari Daisuke Yamashita Tetsuro Inokuma Department of gastroenterology and Hepatology Kobe City Medical Center General HospitalKobe 650-0047HyogoJapan Department of Pathology Kobe City Medical Center General HospitalKobe 650-0047HyogoJapan

BAc.g.OUND chronic hepatitis c virus(HcV)infection is a major global health concern that leads to liver fibrosis,cirrhosis,and *** containing direct-acting antivirals(DAAs)have become the mainstay of HcV treatment,achieving a high sustained virological response(SVR)with minimal adverse *** SUMMARY A 74-year-old woman with chronic HcV infection was treated with the DAAs ledipasvir,and sofosbuvir for 12 wk and achieved ***-four weeks after treatment completion,the liver enzyme and serum Igg levels increased,and antinuclear antibody became positive without HcV viremia,suggesting the development of autoimmune hepatitis(AIH).After liver biopsy indicated AIH,a definite AIH diagnosis was made and prednisolone was *** treatment was effective,and the liver enzyme and serum Igg levels ***,multiple strictures of the intrahepatic and extrahepatic bile ducts with dilatation of the peripheral bile ducts appeared on magnetic resonance cholangiopancreatography after 3 years of achieving SVR,which were consistent with primary sclerosing *** The potential risk of developing autoimmune liver diseases after DAA treatment should be considered.

关键词： Liver Hepatitis c virus Autoimmune hepatitis Primary sclerosing cholangitis Immune system case report

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Self-polarized RgB device realized by semipolar micro-LEDs and perovskite-in-polymer films for backlight applications

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Opto-Electronic Advances 2024年第3期7卷 35-49页

作者： Tingwei Lu Yue Lin Tianqi Zhang Yue Huang Xiaotong Fan Shouqiang Lai Yijun Lu Hao-chung Kuo Zhong chen Tingzhu Wu Rong Zhang School of Electronic Science and Engineering Fujian Engineering Research Center for Solid-State LightingXiamen UniversityXiamen 361005China Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province(IKKEM) Xiamen 361005China Department of Photonics and graduate Institute of Electro-Optical Engineering College of Electrical and Computer EngineeringYang Ming Chiao Tung UniversityHsinchu 30010China Semiconductor Research center Hon Hai Research InstituteTaipei 11492China.

In backlighting systems for liquid crystal displays,conventional red,green,and blue(RgB)light sources that lack polarization properties can result in a significant optical loss of up to 50%when passing through a *** address this inefficiency and optimize energy utilization,this study presents a high-performance device designed for RgB polarized *** device employs an array of semipolar blueμLEDs with inherent polarization capabilities,coupled with mechanically stretched films of green-emitting csPbBr3 nanorods and red-emitting csPbI3-cs4PbI6 hybrid *** csPbBr3 nanorods in the polymer film offer intrinsic polarization emission,while the aligned-wire structures formed by the stable csPbI3-cs4PbI6 hybrid nanocrystals contribute to substantial anisotropic emissions,due to their high dielectric *** resulting device achieved RgB polarization degrees of 0.26,0.48,and 0.38,respectively,and exhibited a broad color gamut,reaching 137.2%of the NTSc standard and 102.5%of the Rec.2020 *** compared to a device utilizing c-plane LEDs for excitation,the current approach increased the intensity of light transmitted through the polarizer by 73.6%.This novel fabrication approach for polarized devices containing RgB components holds considerable promise for advancing next-generation display technologies.

关键词： halide perovskite light-emitting-diodes polarized emission nanocrystals stability

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Transparency and reporting characteristics of randomized controlled trials with chinese herbal medicine formulas interventions

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Frontiers of Medicine 2024年第4期18卷 757-761页

作者： Juan Wang Dongni Shi Yaochen Wang Xuanqi Zhang Han Li Xihong Wang Shufeng Luo Lihan Hu Jiashuai Deng Lin Zhang chung Tai Lau chung Wah cheng Fei Han Ji Li Ping Wang Aiping Lyu Zhaoxiang Bian Xuan Zhang Institute for Research and continuing Education Hong Kong Baptist UniversityShenzhen518057China chinese EQUATOR centre Hong Kong Baptist UniversityHKSAR999077China School of Traditional chinese Medicine Tianjin University of Chinese MedicineTianjin301617China School of chinese Medicine Beijing University of Chinese MedicineBeijing100105China School of chinese Medicine Hong Kong Baptist UniversityHKSAR999077China Department of Pediatrics Guang’anmen HospitalChina Academy of Chinese Medical SciencesBeijing100053China Xiyuan Hospital China Academy of Chinese Medical SciencesBeijing100091China centre for chinese Herbal Medicine Drug Development Hong Kong Baptist UniversityHKSAR999077China

To enhance the clarity and transparency of reporting in trials involving chineseherbal medicine(cHM)formulas,the cONSORT extension specifically tailored for cHM formulas(cONSORT-cHM Formulas 2017)was introduced in 2017[1].This statement,along with its corresponding checklist,outlines key elements essential for reporting in randomized controlled trials(RcTs)related tothe rationale,design,analysis,and interpretation of interventions using cHM *** cONSORT-cHM Formulas 2017 elaborated on 7 of 25 cONSORT checklist items,namely title,abstract and keywords,bac.g.ounds and objectives,participants,interventions,outcomes,generalizability,and interpretation.

关键词： formulas statement rational

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Exploring the Relationship Between Patent Forward citation and Stock Return Rate Using Empirical Data of china Stock Market

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Management Studies 2024年第2期12卷 67-83页

作者： Hong-Wen Tsai Hui-chung che National Taiwan University of Science and Technology TaipeiTaiwanChina china Bio-Medical&Technology Applications Associate New TaipeiTaiwanChina

A novel indicator called price-citation was *** on the company integrated patent database of china listed companies of common stocks(A-shares)with the stock price and the stock return rate data,more than two thousand of A-shares from 2017 to 2020 were *** effect of the traditional patent forward citation and the price-citation for discriminating the stock return rate was thoroughly analyzed via *** A-shares of forward citation counts above the average showed higher stock return rate means than the A-shares having patents but receiving no forward *** price-citation,combining both the financial and patent attributes,defined as the multiplication of the current stock price and the currently receiving forward citation count,showed its excellence in discriminating the stock return *** A-shares of higher price-citation showed significantly higher stock return rate means while the A-shares of lower price-citation showed significantly lowest stock return rate *** price-citation effect had not been changed by cOVID-19 though cOVID-19 affected the social and economic environment to a considerable extent in 2020.

关键词： china A-share patent ANOVA stock return rate forward citation price-citation

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Accelerating Oxygen Electrocatalysis Kinetics on Metal-Organic Frameworks via Bond Length Optimization

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Nano-Micro Letters 2024年第9期16卷 279-290页

作者： Fan He Yingnan Liu Xiaoxuan Yang Yaqi chen cheng‑chieh Yang chung‑Li Dong Qinggang He Bin Yang Zhongjian Li Yongbo Kuang Lecheng Lei Liming Dai Yang Hou Key Laboratory of Biomass chemical Engineering of Ministry of Education College of Chemical and Biological EngineeringZhejiang UniversityHangzhou 310027People’s Republic of China Institute of Zhejiang University-Quzhou Quzhou 324000People’s Republic of China Ningbo Institute of Materials Technology and Engineering Chinese Academy of SciencesNingbo 315201People’s Republic of China School of Biological and chemical Engineering NingboTech UniversityNingbo 315100People’s Republic of China Department of Physics Tamkang UniversityNew Taipei 25137TaiwanPeople’s Republic of China Australian carbon Materials centre(A‑cMc) School of Chemical EngineeringUniversity of New South WalesSydneyNSW 2051Australia

Metal-organic frameworks(MOFs)have been developed as an ideal platform for exploration of the relationship between intrinsic structure and catalytic activity,but the limited catalytic activity and stability has hampered their practical use in water ***,we develop a bond length adjustment strategy for optimizing naphthalene-based MOFs that synthesized by acid etching co-naphthalenedicarboxylic acid-based MOFs(donated as AE-coNDA)to serve as efficient catalyst for water ***-coNDA exhibits a low overpotential of 260 mV to reach 10 mA cm^(−2)and a small Tafel slope of 62 mV dec^(−1)with excellent stability over 100 *** integrated AE-coNDA onto BiVO_(4),photocurrent density of 4.3 mA cm^(−2)is achieved at 1.23 *** investigations demonstrate that the stretched co-O bond length was found to optimize the orbitals hybridization of co 3d and O 2p,which accounts for the fast kinetics and high *** calculations reveal that the stretched co-O bond length strengthens the adsorption of oxygen-contained intermediates at the co active sites for highly efficient water splitting.

关键词： Metal-organic frameworks Bond length adjustment Spin state transition Orbitals hybridization Water splitting

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Superior antibody and membrane protein‑specific T‑cell responses to coronaVac by intradermal versus intramuscular routes in adolescents

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World Journal of Pediatrics 2024年第4期20卷 353-370页

作者： Jaime S.Rosa Duque Samuel M.S.cheng carolyn A.cohen Daniel Leung Xiwei Wang Xiaofeng Mu Yuet chung Tsun Ming Lau Manni Wang Wenyue Zhang Yanmei Zhang Howard H.W.Wong Leo c.H.Tsang Sara chaothai Tsz chun Kwan John K.c.Li Karl c.K.chan Leo L.H.Luk Jenson c.H.Ho Wing Yan Li Amos M.T.Lee Jennifer H.Y.Lam Sau Man chan Wilfred H.S.Wong Issan Y.S.Tam Masashi Mori Sophie A.Valkenburg Malik Peiris Wenwei Tu Yu Lung Lau Department of Paediatrics and Adolescent Medicine The University of Hong KongHong KongChina School of Public Health The University of Hong KongHong KongChina HKU‑Pasteur Research Pole School of Public HealthThe University of Hong KongHong KongChina Research Institute for Bioresources and Biotechnology Ishikawa Prefectural UniversityNonoichiJapan Department of Microbiology and Immunology Peter Doherty Institute for Infectionand ImmunityUniversity of MelbourneMelbourneVICAustralia center for Immunology and Infection c2i Hong KongChina

Bac.g.ound Optimising the immunogenicity of cOVID-19 vaccines to improve their protection against disease is *** dosing by intradermal(ID)administration has been shown to be equally immunogenic as intramuscular(IM)administration for several vaccines,but the immunogenicity of ID inactivated whole severe acute respiratory syndrome coronavirus 2(SARS-coV-2)at the full dose is *** study(NcT04800133)investigated the superiority of antibody and T-cell responses of full-dose coronaVac by ID over IM administration in *** Participants aged 11–17 years received two doses of IM or ID vaccine,followed by the 3rd dose 13–42 days *** and cellular immunogenicity outcomes were measured post-dose 2(IM-cc versus ID-cc)and post-dose 3(IMccc versus ID-ccc).Doses 2 and 3 were administered to 173 and 104 adolescents,*** Spike protein(S)immunoglobulin g(Igg),S-receptor-binding domain(RBD)Igg,S Igg Fcγreceptor IIIa(FcγRIIIa)-binding,SNM[sum of individual(S),nucleocapsid protein(N),and membrane protein(M)peptide pool]-specific interleukin-2(IL-2)+cD4^(+),SNM-specific IL-2^(+)cD8^(+),S-specific IL-2^(+)cD8^(+),N-specific IL-2^(+)cD4^(+),N-specific IL-2^(+)cD8^(+)and M-specific IL-2^(+)cD4^(+)responses fulfilled the superior and non-inferior criteria for ID-cc compared to IM-cc,whereas Igg avidity was *** ID-ccc,S-RBD Igg,surrogate virus neutralisation test,90%plaque reduction neutralisation titre(PRNT90),PRNT50,S Igg avidity,S Igg FcγRIIIa-binding,M-specific IL-2^(+)cD4^(+),interferon-γ+cD8^(+)and IL-2^(+)cD8^(+)responses were superior and non-inferior to *** estimated vaccine efficacies were 49%,52%,66%and 79%for IM-cc,ID-cc,IM-ccc and ID-ccc,*** ID groups reported more local,mild adverse *** This is the first study to demonstrate superior antibody and M-specific T-cell responses by ID inactivated SARScoV-2 vaccination and serves as the basis for future research to improve the

关键词： coronaVac coronavirus disease 2019 Immunity Intradermal Vaccine

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Review Integration of smartphone technology and artificial intelligence for advanced ophthalmic care:A systematic review

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Advances in Ophthalmology Practice and Research 2024年第3期4卷 120-127页

作者： Kai Jin Yingyu Li Hongkang Wu Yih chung Tham Victor Koh Yitian Zhao Ryo Kawasaki Andrzej grzybowski Juan Ye Eye center The Second Affiliated Hospital of Zhejiang University School of MedicineZhejiang Provincial Key Laboratory of OphthalmologyZhejiang Provincial Clinical Research Center for Eye DiseasesZhejiang Provincial Engineering Institute on Eye DiseasesHangzhouChina centre for Innovation and Precision Eye Health National University of SingaporeSingapore Department of Ophthalmology National University of SingaporeSingapore Singapore Eye Research Institute Singapore National Eye CentreSingapore Ophthalmology and Visual Science Academic clinical Program Duke-NUS Medical SchoolSingapore cixi Institute of Biomedical Engineering Ningbo Institute of Materials Technology and EngineeringChinese Academy of SciencesNingboChina Ningbo Eye Hospital NingboChina Zhejiang International Scientific and Technological cooperative Base of Biomedical Materials and Technology Ningbo Institute of Materials Technology and EngineeringChinese Academy of SciencesNingboChina Zhejiang Engineering Research center for Biomedical Materials Ningbo Institute of Materials Technology and EngineeringChinese Academy of SciencesNingboChina Division of Public Health Department of Social MedicineGraduate School of MedicineOsaka UniversityOsakaJapan Artificial Intelligence center for Medical Research and Application Osaka University HospitalOsakaJapan Institute for Research in Ophthalmology Foundation for Ophthalmology DevelopmentPoznanPoland A R T I C L

Bac.g.ound:The convergence of smartphone technology and artificial intelligence(AI)has revolutionized the landscape of ophthalmic care,offering unprecedented opportunities for diagnosis,monitoring,and management of ocular ***,there is a lack of systematic studies on discussing the integration of smart-phone and AI in this *** text:This review includes 52 studies,and explores the integration of smartphones and AI in ophthalmology,delineating its collective impact on screening methodologies,disease detection,telemedicine initiatives,and patient *** collective findings from the curated studies indicate promising performance of the smartphone-based AI screening for various ocular diseases which encompass major retinal diseases,glaucoma,cataract,visual impairment in children and ocular surface ***,the utilization of smartphone-based imaging modalities,coupled with AI algorithms,is able to provide timely,efficient and cost-effective screening for ocular *** modality can also facilitate patient self-monitoring,remote patient monitoring and enhancing accessibility to eye care services,particularly in underserved *** involving data pri-vacy,algorithm validation,regulatory frameworks and issues of trust are still need to be ***,evaluation on real-world implementation is imperative as well,and real-world prospective studies are currently ***:Smartphone ocular imaging merged with AI enables earlier,precise diagnoses,personalized treat-ments,and enhanced service accessibility in eye *** is crucial to navigate ethical and data security challenges while responsibly leveraging these innovations,promising a potential revolution in care access and global eye health equity.

关键词： Artificial intelligence Smartphone imaging Ophthalmology Telemedicine Ocular disease management

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Distinct molecular targets of ProEgcg from Egcg and superior inhibition of angiogenesis signaling pathways for treatment of endometriosis

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Journal of Pharmaceutical Analysis 2024年第1期14卷 100-114页

作者： Sze Wan Hung Massimiliano gaetani Yiran Li Zhouyurong Tan Xu Zheng Ruizhe Zhang Yang Ding gene chi Wai Man Tao Zhang Yi Song Yao Wang Jacqueline Pui Wah chung Tak Hang chan Roman A.Zubarev chi chiu Wang Department of Obstetrics&gynaecology The Chinese University of Hong KongHong KongChina Division of Physiological chemistry I Department of Medical Biochemistry and BiophysicsKarolinska InstitutetStockholmSE 17177Sweden chemical Proteomics core Facility Department of Medical Biochemistry and BiophysicsKarolinska InstitutetStockholmSE 17177Sweden Unit of chemical Proteomics Science for Life Laboratory(SciLifeLab)StockholmSE 17177Sweden center for Reproductive Medicine Henan Key Laboratory of Reproduction and GeneticsThe First Affiliated Hospital of Zhengzhou UniversityZhengzhou450003China Department of chemistry McGill UniversityMontrealH3A2K6Canada Department of Pharmacological&Technological chemistry I.M.Sechenov First Moscow State Medical UniversityMoscow119146Russia Reproduction and Development Li Ka Shing Institute of Health SciencesThe Chinese University of Hong KongHong KongChina School of Biomedical Sciences The Chinese University of Hong KongHong KongChina chinese University of Hong Kong-Sichuan University Joint Laboratory in Reproductive Medicine The Chinese University of Hong KongHong KongChina

Endometriosis is a common chronic.g.necological disease with endometrial cell implantation outside the *** is a major pathophysiology in *** previous studies have demonstrated that the prodrug of epigallocatechin gallate(ProEgcg)exhibits superior anti-endometriotic and anti-angiogenic effects compared to epigallocatechin gallate(Egcg).However,their direct binding targets and underlying mechanisms for the differential effects remain *** this study,we demonstrated that oral ProEgcg can be effective in preventing and treating ***,1D and 2D Proteome Integral Solubility Alteration assay-based chemical proteomics identified metadherin(MTDH)and PX domain containing serine/threonine kinase-like(PXK)as novel binding targets of Egcg and ProEgcg,*** simulation and BioLayer interferometry were used to confirm their binding *** results showed that MTDH-Egcg inhibited protein kinase B(Akt)-mediated angiogenesis,while PXK-ProEgcg inhibited epidermal growth factor(EgF)-mediated angiogenesis via the EgF/hypoxia-inducible factor(HIF-1a)/vascular endothelial growth factor(VEgF)*** vitro and in vivo knockdown assays and microvascular network imaging further confirmed the involvement of these signaling ***,our study demonstrated that ProEgcg has superior therapeutic effects than Egcg by targeting distinct signal transduction pathways and may act as a novel antiangiogenic therapy for endometriosis.

关键词： Molecular targets ProEgcg Egcg Angiogenesis Treatment Endometriosis

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Integration of 1H-NMR-Based Metabolomics Approach with Traditional chinese Pulse Diagnosis to Evaluate the Qi-Enhancing Activity of Herbal Tea

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chinese Medicine 2024年第3期15卷 49-74页

作者： chia-Hsin Lin Po-Jung Huang chung-Hsuan Wang Mo-Rong Xu Sheng-Yang Wang Department of Forestry Chung Hsing University Taichung Fu Kang Traditional chinese Medicine clinic Nantou Special crop and Metabolome Discipline cluster Academy Circle Economy Chung Hsing University Taichung Agricultural Biotechnology Research Institute Academia Sinica Taipei City

Human metabolism is intricately linked to an individual’s health status. Regardless of living habits, it will be reflected in the metabolic characteristics of urine. The utilization of the 1H NMR-based metabolomics method has enabled examine the metabolomic changes in urine under various physiology conditions, providing valuable insights into metabolites. In this particular study, volunteers were divided into two groups based on the strength of their spleen pulses, using the pulse diagnosis method employed in traditional chinese medicine. Subsequently, their urine samples were analyzed, revealing notable variances in urea, creatinine, citric acid, succinic acid, trimethylamine-N-oxide (TMAO), alanine, hippuric acid, and glycine between the two groups. Interestingly, individuals with weak spleen pulses showed significant improvements after consuming herbal tea. Furthermore, we conducted Lc-MS analysis on herbal tea and performed adenosine triphosphate (ATP) activity tests on the c2c12 mouse skeletal muscle cell line. The results indicated that within a reasonable concentration range, exposure to herbal tea led to an increase in the mitochondrial ATP production capacity of c2c12 cells. These findings shed light on the relationship between traditional chinese medicine pulse diagnosis and urine metabolites, highlighting their potential as non-invasive and straightforward health assessment indicators. They can aid in the preliminary determination of necessary dietary and lifestyle changes to enhance overall bodily health.

关键词： 1H-NMR Metabolomics Traditional chinese Pulse Diagnosis Qi-Enhancing Herbal Tea

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