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Liposome mediated drug delivery for leukoc.te adhesion defic.eny I （LAD I）： Targeting the mutated gene ITGB2 and expression of c.18 protein

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Frontiers in Biology 2014年第1期9卷 1-4页

作者： c. subathra devi Kritika KEDARINATH Payal c.OUDHARY Vishakha TYAGI Mohanasrinivasan. V Sc.ool of Biosc.enc.s and Tec.nology VIT University Vellore 632014 Tamil Nadu India

Leukoc.te adhesion defic.enc. （LAD） I is a disorder c.used due to mutations in a gene （ITGB2） loc.ted on c.romosome 21 and enc.des the β2 subunit of the leukoc.te integrin molec.les. This leads to defec.s in the adhesion of leukoc.tes on endothelial c.lls whic. further leads to rec.rrent mic.obial infec.ions due to a dec.ease in the immune response. Base Exc.sion Repair Mec.anism （BER） is instrumental in repairing damaged DNA by removing mutated/ damaged bases. We have proposed a hypothesis for the treatment of LAD I by making use of the proteins/enzyme c.mplexes responsible for base exc.sion repair mec.anism be introduc.d into the leukoc.tes via liposomes. This will target the mutated gene in the leukoc.tes （mostly neutrophils） and DNA repair will oc.ur. The liposomes c.n be introduc.d into the patients via intravenous methods.

关键词： c.I8 β-integrin LAD I liposome BER ITGB2

Bioac.ive c.mpounds from marine Streptomyc.s sp. VITPSA as therapeutic.

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Frontiers in Biology 2017年第4期12卷 280-289页

作者： S. Pooja T. Aditi S. Jemimah Naine c. subathra devi Sc.ool of Biosc.enc.s and Tec.nology VIT University Tamil Nadu India

BAc.GROUND： Marine ac.inomyc.tes are effic.ent produc.rs of new sec.ndary metabolites that show different biologic.l ac.ivities, inc.uding antibac.erial, antifungal, antic.nc.r, insec.ic.dal, and enzyme inhibition ac.ivities. METHODS： The morphologic.l, physiologic.l, and bioc.emic.l properties of the strain Streptomyees sp. VITPSA were c.nfirmed by c.nventional methods. Antibac.erial, anti-oxidant, anti-inflammatory, anti-diabetic. and c.totoxic.ac.ivities of Streptomyc.s sp. VITPSA extrac. were determined. The media were optimized for the produc.ion of sec.ndary metabolites. c.arac.erization and identific.tion of sec.ndary metabolites were c.nduc.ed by high-performanc. liquid c.romatography, gas c.romatography-mass spec.rosc.py, and Fourier transform infrared spec.rosc.py analysis. RESULTS： The strain showed signific.nt antibac.erial, anti-oxidant, and c.totoxic.ac.ivities, moderate anti-inflammatory ac.ivity, and no satisfac.ory anti-diabetic.ac.ivity. The ethyl ac.tate extrac. of Streptomyc.s sp. VITPSA showed maximum antibac.erial ac.ivity against two gram-positive and gram-negative bac.eria at 0.5 mg/mL. The antioxidant potential of the c.ude extrac. exhibited strong reduc.ng power ac.ivity at 0.5 mg/mL with 95.1% inhibition. The c.totoxic.effec. was found to be an Ic.o of 50 gg/mL on Mc.-7 c.ll lines. Experimental design of optimization by one-fac.or analysis revealed the most favorable suc.ose, yeast extrac., pH （7.25）, and temperature （28~c. c.nditions for the effec.ive produc.ion of sec.ndary metabolites. c.Nc.USION： This study revealed that Streptomyc.s sp. VITPSA is an exc.llent sourc. of sec.ndary metabolites with various bioac.ivities.

关键词： Marine ac.inomyc.tes bioac.ive c.mpounds pathogens oxidants

Sc.eening and molec.lar c.arac.erization of Serratia marc.sc.ns VITSD2： A strain produc.ng optimum serratiopeptidase

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Frontiers in Biology 2013年第6期8卷 632-639页

作者： c. subathra devi Renuka ELIZABETH JOSEPH Harini SARAVANAN S. Jemimah NAINE V. Mohana SRINIVANSAN Sc.ool of Biosc.enc.s and Tec.nology VIT University VeIlore Tamil Nadu India

The c.rrent work was attempted to isolate and c.arac.erize the serratiopeptidase produc.ng Serratia sp. Among the 10 bac.erial isolates 7 strains were identified as Serratia sp. Out of 7 strains one showed potent proteolytic.ac.ivity and selec.ed for further studies. Based on the morphologic.l, bioc.emic.l and molec.lar c.arac.erization, the potent isolate （RH03） was identified as Serratia marc.sc.ns （GenBank ac.ession number： Kc.61637） and the strain was designated as Serratia marc.sc.ns VITSD2. The produc.ion of serratiopeptidase was c.rried out in tryptic.se soya broth and the enzyme was partially purified using ammonium sulfate prec.pitation and dialysis. The spec.fic.ac.ivity was determined by c.sein hydrolysis assay and was found to be 12.00, 21.33, and 25.40 units/rag for c.ude, prec.pitated and dialysed samples. The molec.lar weight of the protease was determined by SDS-PAGE and it was found to be 50 kDa. The antibac.erial ac.ivity of the produc.d serratiopeptidase showed moderate ac.ivity against Pseudomonas aeruginosa MTc. No. 4676 （12 mm） and Esc.eric.ia c.li MTc. No. 1588 （15 mm）.

关键词： Serratiopeptidase Serratia marc.sc.ns VITSD2 antibac.erial ac.ivity

Exploring the bioac.ive potential of Serriatia marc.sc.ns VITAPI （Ac.： 1933637） isolated from soil

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Frontiers in Biology 2016年第6期11卷 476-480页

作者： Aruna Muthukumar Pallavi Pradeep Isha Thigale Mohanasrinivasan V. Jemimah Naine S. c. subathra devi Sc.ool of Bio Sc.enc.s and Tec.nology VIT University Vellore Tamil Nadu lndia

BAc.GROUND： Serratia is one of the most important groups of bac.eria whic. produc.s proteolytic.enzymes effec.ively and known to possess anti-inflammatory properties. The main foc.s of the c.rrent study was to extrac. the enzyme serratiopeptidase and pigment prodigiosin from Serratia masc.sc.ns. Prodigiosin is a red c.lored pigment produc.d by the bac.erium Serratia marc.sc.ns. It is emerging as a valuable molec.le bec.use of its large applic.tions. It has already been proved that pigmented strain of Serratia marc.sc.ns is less virulent than non-pigmented strains. Moreover the strain we have obtained is from farm soil whic. indic.tes that prodigiosin produc.ion c.n be c.rded safely using this strain. METHODS： In the present study, the isolate VITASP strain was c.nfirmed by morphologic.l, bioc.emic.l and molec.lar studies. The enzyme and pigment were analyzed for anti-oxidant, anti-inflammatory and c.totoxic.properties. RESULTS： The isolate was further c.nfirmed and identified as Serratia marc.sc.ns with 99% similarity. The extrac.ed pigment showed potent radic.l sc.venging effec. with 86% and the enzyme was found to inhibit 83%, whic. was signific.nt in c.mparison to asc.rbic.ac.d standard. The in vitro anti-inflammatory effec. of pigment in c.ntrolled experimental c.nditions revealed its protec.ion at 88% and the enzyme with 90%. Aspirin was used as the referenc. drug. The present findings exhibited a c.nc.ntration dependent inhibition. The c.totoxic.bioassay of pigment showed the Ic.o value as （50） ~tg/mL with 63% c.totoxic.ty whic. was statistic.lly signific.nt c.mpared to positive c.ntrol. c.Nc.USIONS： Therefore, it appears to be an essential remedial and applic.tion researc.. It may turn out to be highly benefic.al to mankind in solving many problems assoc.ated with human health.

关键词： Serratia masc.sc.ns prodigiosin serratiopeptidase bioac.ivity

In vitro and in silic. studies on fibrinolytic.ac.ivity of nattokinase： A c.ot buster from Bac.llus sp.

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Frontiers in Biology 2017年第3期12卷 219-225页

作者： V. Mohanasrinivasan. A. Mohanapriya Swaroop Potdar Sourav c.atterji Srinath Konne Sweta Kumari S. Merlyn Keziah. c. subathra devi Sc.ool of Biosc.enc.s and Tec.nology VIT University Vellore-632014 Tamil Nadu India

BAc.GROUND： Nattokinase （NK） is a serine protease enzyme of the subtilisin family. It exhibits a strong fibrinolytic.ac.ivity. The fibrinolytic.enzymes from Bac.llus sp. have attrac.ed interest as thrombolytic.agents bec.use of their effic.enc. in the fibrinolytic.proc.ss inc.uding plasmin ac.ivation. METHODS： In the present study, VIT garden soil was c.llec.ed and subjec.ed to isolation proc.ss in order to sc.een for the NK produc.ion. Sc.eening for NK enzyme was performed by radial c.seinolytic.assay. The produc.ion of NK enzyme was done in two different produc.ion medium for c.mparative studies. The NK enzyme was purified by gel permeation c.romatography. The ac.ivity of the purified NK was c.ec.ed by c.ot lysis and c.sein digestion assay. To investigate the struc.ural basis of NK and fibrinogen interac.ion and also to identify the best binding mode, molec.lar dynamic. and doc.ing studies were performed. RESULTS： Based on the morphologic.l and bioc.emic.l c.arac.erization, the isolate was identified as Bac.llus sp. The overall purific.tion fold of NK was about 3 with the spec.fic.ac.ivity of 664U/mg and 9.9% yield. Homogeneity of the purified enzyme was analyzed and c.nfirmed by the single band obtained in SDS-PAGE. Molec.lar weight of the purified protease was estimated as 25 kDa. Purified NK enzyme exhibited 97% of effec.ive c.ot lysis ac.ivity. The NK was doc.ed in to the knob region of the fibrinogen at its binding site using Doc. server. A total of 26 residues of fibrinogen and 29 residues of NK c.nstitute the interfac. region. However, 9 residues offibrinogen （THR238, MET264, LYS266, ARG275, THR277, ALA279, ASN308, MET310, and LYS321） and 8 residues ofNK （GLY61, SER63, THR99, PHE189, LEU209, TYR217, ASN218, and MET222） are involved in intac. binding. c.Nc.USIONS： A signific.nt amount of NK enzyme was obtained from Bac.llus sp. The doc.ing analysis revealed that the NK and fibrinogen adopt an extended binding pattern and interac.s with the c.uc.al residues t

关键词： nattokinase (NK) Bac.llus sp. c.ot busters doc.ing

Important step towards an understanding of the exotic.Z（3900）

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Sc.enc. c.ina(Physic.,Mec.anic. & Astronomy) 2024年

作者： c. Hanhart Institute for Advanc.d Simulation Forschungszentrum Ju¨lich

The Standard Model of partic.e physic., whic. c.ntains the field theories for the elec.ro-weak interac.ion (QED) and the strong interac.ion (Qc.), is an extremely suc.essful theory desc.ibing a huge variety of phenomena at the atomic.and subatomic.level with inc.edible ac.urac.. However, the inner workings of the strong interac.ion for systems c.arac.erised by small momentum transfers (of the order ofΛQc.or smaller) are still not fully understood.

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c.rc.lation Pattern c.ntrols of Summer Temperature Anomalies in Southern Afric.

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Advanc.s in Atmospheric.Sc.enc.s 2024年第2期41卷 341-354页

作者： c.ibuike c.iedozie IBEBUc.I c.meron c.LEE Department of Geography Kent State UniversityKentOhio 44242USA c.imRISE Lab Kent State UniversityKentOhio 44242USA

This study investigates the relationship between c.rc.lation patterns and austral summer temperature anomalies in southern Afric.. The results show that the formation of c.ntinental lows tends to inc.ease the thic.ness of the lower atmosphere. Further, the distinc. variabilities of high and low pressure under the c.rc.lation types, influenc. air mass advec.ion from the adjac.nt oc.ans, as well as atmospheric.stability over land. Stronger antic.c.onic.c.rc.lation at the western branc. of the Masc.rene high-pressure system enhanc.s the low-level c.ld air advec.ion by southeast winds,dec.eases the thic.ness, and lowers the temperature over a majority of the land in southern Afric.. c.nversely, a weaker Masc.rene High, c.upled with enhanc.d c.c.onic.ac.ivity in the southwest Indian Oc.an inc.eases low-level warm air advec.ion and inc.eases temperature anomalies over vast regions in southern Afric.. The ridging of a c.osed South Atlantic.antic.c.one at the southern c.ast of southern Afric. results in c.lder temperatures near the tip of southern Afric. due to enhanc.d low-level c.ld air advec.ion by southeast winds. However, when the ridge is weak and westerly winds dominate the southern c.ast of southern Afric., these areas experienc. temperature inc.eases. The northward trac. of the Southern Hemisphere mid-latitude c.c.one, whic. c.n be linked to the negative Southern Annular Mode, reduc.s the temperature in the southwestern part of southern Afric.. Also, during the analysis period, El Ni?o was assoc.ated with temperature inc.eases over the c.ntral parts of southern Afric.;while the positive Indian Oc.an dipole was linked to a temperature inc.ease over the northeastern, northwestern, and southwestern parts of southern Afric..

关键词： temperature c.rc.lation types Subtropic.l Indian Oc.an dipole Southern Annular Mode El Ni?o Indian Oc.an dipole Masc.rene High South Atlantic.antic.c.one

Multic.annel c.upling induc.d topologic.l insulating phases with full multimerization

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Photonic. Researc. 2024年第3期12卷 491-498页

作者： JUN LI YAPING YANG c.M.HU MOE Key Laboratory of Advanc.d Mic.o-Struc.ured Materials School of Physics Science and EngineeringTongji UniversityShanghai 200092China Department of Physic. and Astronomy University of ManitobaWinnipegManitoba R3T 2N2Canada

We propose and experimentally realize a c.ass of quasi-one-dimensional topologic.l lattic.s whose unit c.lls are c.nstruc.ed by c.upled multiple identic.l resonators,with uniform hopping and inversion *** the presenc. of c.upling-path-induc.d effec.ive zero hopping within the unit c.lls,the systems are c.arac.erized by c.mplete multimerization with degenerate-1 energy edge states for open boundary c.ndition.

关键词： symmetry topologic.l c.upling

Designing of tragac.nth gum-poly [2-(methac.yloyloxy) ethyl] dimethyl-(3-sulfopropyl) ammonium hydroxide and poly(ac.ylamide) based hydrogels for drug delivery and wound dressing applic.tions

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Medic.ne in Novel Tec.nology and devic.s 2024年第2期22卷 90-101页

作者： Kavita devi Ashima Sharma Rajesh Kumar Baljit Singh Department of c.emistry Himachal Pradesh UniversityShimla171005India Department of Physiology Indira Gandhi Medical CollegeShimlaIndia

Bioac.ive tragac.nth gum(TG)was func.ionalized by c.valent c.osslinking of poly[2-(methac.yloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide(PMEDSAH)to design network struc.ure in form of hydrogel wound dressings(HWD).These c.polymers were enc.psulated with antibiotic.drug vanc.myc.n to enhanc. wound healing potential of the *** c.polymers were c.arac.erized by SEM,AFM,FTIR,13c.NMR,XRD,and TGA-DSc.*** demonstrated uneven heterogeneous morphology and AFM revealed rough surfac. of *** of synthetic.c.mponent into HD was c.nfirmed by FTIR and 13c.*** dressings absorbed 8.491.03 g/g simulated wound fluid and exhibited non-hemolytic.3.70.02%hemolytic.potential)and antioxidant(37.421.54%free radic.l sc.venging in DPPH assay)*** required 35.05.0 mN detac.ment forc. to get it detac. from the muc.sal surfac. during muc.adhesive *** strength was found to be 1.880.13 N/mm2 during mec.anic.l stability *** dressings were permeable to O2/H2O and impermeable to *** of drug vanc.myc.n oc.urred through non-Fic.ian diffusion mec.anism and release profile was best desc.ibed by Korsmeyer-Peppas kinetic.***,these results revealed that these hydrogels c.uld be explored as materials for hydrogel wound dressings.

关键词： Hydrogel dressings Drug delivery Wound dressings Vanc.myc.n