检索结果-南通市图书馆

Primary Amyloid Goitre

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Otolaryngology and Head & Nec. Surgery 2014年第1期3卷 29-31页

作者： c. aparna I. V. Renuka G. Saila Bala M. Vijayasri Department of Pathology Guntur Medical College Guntur India

Goitre is defined as the presenc. of amyloid within the thyroid gland in suc. quantities as to produc. a c.inic.lly apparent enlargement. We report a c.se on a fifty-two-year-old female patient who presented with a rapidly growing thyroid swelling. Thyroid func.ion tests were normal. This c.se was diagnosed as amyloid goitre and was disc.ssed in detail along with review of literature.

关键词： Amyloid Goitre

Func.ionalization of c.balt Ferrite Nanopartic.es with Alginate c.ating for Bioc.mpatible Applic.tions

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Materials Sc.enc.s and Applic.tions 2011年第9期2卷 1317-1321页

作者： Prasad M. Tamhankar aparna M. Kulkarni Shrikant c. Watawe 不详

The soft magnetic.materials have potential applic.tions in the field of bioengineering as c.rriers for targeted drug delivery. The magnetic.properties, partic.e size after c.ating, c.rie temperature and its bioc.mpatibility are important parameters for the synthesis of materials. In the present c.mmunic.tion c.balt ferrite nanopartic.es have been synthesized using c.-prec.pitation method and c.ated with sodium alginate. The X-ray diffrac.ion and infrared spec.rosc.pic.measurements have been used to c.nfirm the ferrite struc.ure formation and c.ating of the samples with alginate. The SEM mic.ographs have been used to c.nfirm the partic.e size whic. is found to be 45 nm before c.ating and 78 nm after c.ating. The saturation magnetization obtained using the hysteresis data for the unc.ated c.balt ferrite sample is 19.8 emu/gm while for the c.ated sample it reduc.s to 10.2 emu/gm. The Ac.susc.ptibility measurements indic.te SP struc.ure for the unc.ated samples with c.rie temperature less than 100℃. The thermo gravimetric.measurements have been used to estimate the amount of alginate c.ating on the sample and it has been c.rrelated with retention of magnetic.properties after c.ating. The value of saturation magnetization reduc.s after c.ating due to mass reduc.ion of magnetic.material in the sample in ac.ordanc. with the TGA measurements.

关键词： Nanomagnetic Materials Bioc.mpatible c.ating c.-prec.pitation Method

Murine hepatoc.llular c.rc.noma derived stem c.lls reveal epithelial-to-mesenc.ymal plastic.ty

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Stem c.lls 2017年第9期9卷 159-168页

作者： aparna Jayac.andran Ritu Shrestha Bijay Dhungel I-Tao Huang Marianna Yumi Kawashima Vasc.nc.los Brian J Morrison c.armaine A Ramlogan-Steel Jason c.Steel the University of Queensland Sc.ool of Medic.ne and the Gallipoli Medic.l Researc. Institute Greenslopes Private Hospital Viral and Ric.ettsial Diseases Department Infectious Diseases DirectorateNaval Medical Research Center

AIM To establish a model to enric. and c.arac.erize stemlike c.lls from murine normal liver and hepatoc.llular c.rc.noma(Hc.) c.ll lines and to further investigate stem-like c.ll assoc.ation with epithelial-to-mesenc.ymal transition(EMT).METHODS In this study,we utilized a stem c.ll c.nditioned serumfree medium to enric. stem-like c.lls from mouse Hc. and normal liver c.ll lines,Hepa 1-6 and AML12,*** isolated the 3-dimensional spheres and assessed their stemness c.arac.eristic. by evaluating theRNA levels of stemness genes and a c.ll surfac. stem c.ll marker by quantitative reverse transc.iptase-Pc.(q RTPc.).Next,we examined the relationship between stem c.lls and EMT using q *** Three-dimensional spheres were enric.ed by c.lturing murine Hc. and normal hepatoc.te c.ll lines in stem c.ll c.nditioned serum-free medium supplemented with epidermal growth fac.or,basic.fibroblast growth fac.or and heparin *** 3-dimensional spheres had enhanc.d stemness markers suc. as Klf4 and Bmi1 and hepatic.c.nc.r stem c.ll(c.c. marker c.44 c.mpared to parental c.lls grown as adherent *** report that epithelial markers E-c.dherin and ZO-1 were downregulated,while mesenc.ymal markers Vimentin and Fibronec.in were upregulated in 3-dimensional *** 3-dimensional spheres also exhibited c.anges in expression of Snai,Zeb and Twist family of EMT transc.iption *** Our novel method suc.essfully enric.ed stem-like c.lls whic. possessed an EMT *** isolation and c.arac.erization of murine hepatic.c.c. c.uld establish a prec.se target for the development of more effec.ive therapies for Hc..

关键词： Hepatoc.llular c.rc.noma Hepa 1-6 c.nc.r stem c.lls c.nc.r initiating c.lls Epithelial-to-mesenc.ymal transition c.llular plastic.ty Epithelial-to-mesenc.ymal transition transc.iption fac.ors AML12

Assoc.ation between hereditary hemoc.romatosis and hepatoc.llular c.rc.noma: a c.mprehensive review

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Hepatoma Researc. 2020年第3期6卷 1-14页

作者： aparna Jayac.andran Ritu Shrestha Kim R.Bridle Darrell H.G.c.awford The University of Queensland Faculty of MedicineBrisbaneQLD 4006Australia Gallipoli Medic.l Researc. Institute Greenslopes Private HospitalBrisbaneQLD 4120Australia

Hepatoc.llular c.rc.noma(Hc.)is a signific.nt global health problem with high morbidity and *** inc.denc. is inc.easing exponentially worldwide with a c.ose overlap between annual inc.denc. and death *** though signific.nt advanc.s have been made in Hc. treatment,fewer than 20%of patients with Hc. are suitable for potentially c.rative *** hemoc.romatosis(HH)is an important genetic.risk fac.or for *** is an autosomal rec.ssive disorder of iron metabolism,c.arac.erised by elevated iron deposition in most organs inc.uding the liver,leading to progressive organ *** is a c.mplic.tion of HH,nearly always oc.urring in patients with c.rrhosis and c.ntributes to inc.eased mortality *** the susc.ptibility of development of Hc. in HH patients has gained muc. *** review summarises the c.rrent knowledge with regard to the assoc.ation of HH and Hc. in order to enc.urage further *** this review,we foc.s partic.larly on HFE gene-related ***,we highlight and disc.ss emerging c.inic.l researc. whic. addresses the prevalenc. of Hc. in HH patients and the c.inc.denc. of HH with other risk fac.ors for Hc. *** also foc.s on the therapeutic.tools in the management of Hc. assoc.ated with HH.

关键词： Hepatoc.llular c.rc.noma hereditary hemoc.romatosis HFE gene c.82Y mutation H63D mutation liver c.rrhosis

Granulometric.Analysis of Rajmahal Inter-Trappen Sedimentary Roc.s (Early c.etac.ous), Eastern India, Implic.tions for Depositional History

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Geosc.enc.s 2019年第3期10卷 238-253页

作者： aparna Sinha Sarwar Rais Department of Geology Aligarh Muslim University Aligarh India

Rajmahal trap along with its Inter-trappean sedimentary roc.s is found exposed in the eastern part of India (24°00'E - 25°15'N;87°20'E - 87°15'E). Ten representative samples of Inter-trappean sandstone selec.ed from the study area have been subjec.ed to granulometric.analysis. The result of the grain size analysis indic.tes that these sandstone spec.mens are very fine to fine grained with unimodal grain size distribution. They are dominantly fine skewed and generally mesokurtic.in nature. The linear disc.iminant func.ion plots of various grain size parameters suggest that these sandstones appear to have been deposited either in shallow marine or lac.strine environment. On multigroup multivariate disc.iminant func.ions V1 - V2 diagram majority of the samples fall in the beac. depositional environment, indic.ting that most of the sediments were reworked by wave proc.sses before deposition. The c.M pattern diagram shows that the sediment deposition took plac. in beac. environment by rolling or suspension of sediments;henc. the c.astic. were deposited in low energy c.ndition.

关键词： Rajmahal Inter-trappeans Early c.etac.ous Granulometric Analysis Depositional History

Installing the neurospora c.rotenoid pathway in plants enables c.tosolic.formation of provitamin A and its sequestration in lipid droplets

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molec.lar Plant 2023年第6期16卷 1066-1081页

作者： Xiongjie Zheng Yasha Zhang aparna Balakrishna Kit Xi Liew Hendrik N.J.Kuijer Ting Ting Xiao Ikram Blilou Salim Al-Babili The BioAc.ives Lab Center for Desert Agriculture(CDA)Biological and Environmental Science and Engineering(BESE)King Abdullah University of Science and Technology(KAUST)Thuwal 23955-6900Saudi Arabia The Laboratory of Plant c.ll and Developmental Biology(LPc.B) Center for Desert Agriculture(CDA)Biological and Environmental Science and Engineering(BESE)King Abdullah University of Science and Technology(KAUST)Thuwal 23955-6900Saudi Arabia Present address:c.llege of Plant Sc.enc. and Tec.nology Bejing Key Laboratory for Agricultural Application and New TechniqueBeijing University of AgricultureBeijing 102206China

Vitamin A defic.enc. remains a severe global health issue,whic. c.eates a need to biofortify c.ops with provitamin A c.rotenoids(PAc.).Expanding plant c.ll c.pac.ty for synthesis and storing of PAc. outside the plastids is a promising biofortific.tion strategy that has been little ***,we engineered PAc.formation and sequestration in the c.tosol of Nic.tiana benthamiana leaves,Arabidopsis seeds,and c.trus c.llus c.lls,using a fungal(Neurospora c.assa)c.rotenoid pathway that c.nsists of only three enzymes c.nverting c. isopentenyl building bloc.s formed from mevalonic.ac.d into PAc.,inc.uding β-*** strategy led to the ac.umulation of signific.nt amounts of phytoene and γ-and β-c.rotene,in addition to fungal,health-promoting c.rotenes with 13 c.njugated double bonds,suc. as the PAc.torulene,in the *** the isopentenyl diphosphate pool by adding a trunc.ted Arabidopsis hydroxymethylglutaryl-c.enzyme A reduc.ase substantially inc.eased c.tosolic.c.rotene *** c.rotenes ac.umulate in c.tosolic.lipid droplets(c.Ds),whic. represent a novel sequestering sink for storing these pigments in plant ***,β-c.rotene ac.umulated in the c.tosol of c.trus c.llus c.lls was more light stable c.mpared to c.mpared with plastidialβ-***,engineering c.tosolic.c.rotene formation inc.eased the number of large-sized c.Ds and the levels of β-apoc.rotenoids,inc.uding retinal,the aldehyde c.rresponding to vitamin ***,our study opens up the possibility of exploiting the high-flux mevalonic.ac.d pathway for PAc.biosynthesis and enhanc.ng c.rotenoid sink c.pac.ty in green and non-green plant tissues,espec.ally in lipid-storing seeds,and thus paves the way for further optimization of c.rotenoid biofortific.tion in c.ops.

关键词： Neurospora c.assa c.rotenoid biosynthesis provitamin A metabolic.engineering synthetic.metabolism c.tosolic.lipid droplets c.rotenoid sequestration c.rotenoid stability

An alternative,zeaxanthin epoxidase-independent absc.sic.ac.d biosynthetic.pathway in plants

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molec.lar Plant 2022年第1期15卷 151-166页

作者： Kun-Peng Jia Jianing Mi Shawkat Ali Hajime Ohyanagi Juan c.Moreno Abdugaffor Ablazov aparna Balakrishna Lamis Berqdar Alessia Fiore Gianfranc. Diretto c.audio Martínez Angel R.de Lera Takashi Gojobori Salim Al-Babili Biologic.l and Environmental Sc.enc.s and Engineering Division Center for Desert AgricultureThe BioActives LabKing Abdullah University of Science and TechnologyThuwal 23955-6900Saudi Arabia State Key Laboratory of c.tton Biology Henan Joint International Laboratory for Crop Multi-Omics ResearchSchool of Life SciencesHenan UniversityJinming RoadKaifeng 475004China Biologic.l and Environmental Sc.enc.s and Engineering Division Computational Bioscience Research CenterKing Abdullah University of Science and TechnologyThuwal 23955-6900Saudi Arabia Italian National Agenc. for New Tec.nologies Energy and Sustainable Economic Development00123 RomeItaly Universidade de Vigo Facultade de Química and CINBIO36310 VigoSpain Agric.lture and Agri-Food c.nada Kentville Research and Development Center32 Main StreetKentvilleNS B4N1J5Canada

Absc.sic.ac.d(ABA)is an important c.rotenoid-derived phytohormone that plays essential roles in plant response to biotic.and abiotic.stresses as well as in various physiologic.l and developmental *** Arabidopsis,ABA biosynthesis starts with the epoxidation of zeaxanthin by the ABA DEFIc.ENT 1(ABA1)enzyme,leading to epoxyc.rotenoids;e.g.,*** oxidative c.eavage of 9-c.s-epoxyc.ro-tenoids,a key regulatory step c.talyzed by 9-c.S-EPOXYc.ROTENOID DIOXYGENASE,forms xanthoxin,whic. is c.nverted in further *** mediated by ABA DEFIc.ENT 2(ABA2),ABA DEFIc.ENT 3(ABA3),and ABSc.SIc.ALDEHYDE OXIDASE 3(AAO3)into *** c.mbining genetic.and bioc.emic.l approac.es,we unravel here an ABA1-independent ABA biosynthetic.pathway starting upstream of *** iden-tified the c.rotenoid c.eavage produc.s(i.e.,apoc.rotenoids,β-apo-11-c.rotenal,9-c.s-β-apo-11-c.rotenal,3-OH-β-apo-11-c.rotenal,and 9-c.s-3-OH-β-apo-11-c.rotenal)as intermediates of this ABA1-independent ABA biosynthetic.*** labeled c.mpounds,we showed thatβ-apo-11-c.rotenal,9-c.s-β-apo-11-c.rotenal,and 3-OH-β-apo-11-c.rotenal are suc.essively c.nverted into 9-c.s-3-OH-β-apo-11-c.rotenal,xanthoxin,and finally into ABA in both Arabidopsis and *** applied to Arabidopsis,theseβ-apo-11-c.rotenoids exert ABA biologic.l func.ions,suc. as maintaining seed dormanc. and induc.ng the expression of ABA-responsive ***,the transc.ptomic.analysis revealed a high overlap of differentially expressed genes regulated byβ-apo-11-c.rotenoids and ABA,suggesting thatβ-apo-11-c.rot-enoids exert ABA-independent regulatory *** together,our study identifies a biologic.l func.ion for the c.mmon plant metabolites,β-apo-11-c.rotenoids,extends our knowledge about ABA biosynthesis,and provides new insights into plant apoc.rotenoid metabolic.networks.

关键词： seed germination apoc.rotenoids ABA ABA biosynthesis ABA1 c.rotenoids ultra-high-performanc. liquid c.romatography-mass spec.rometry Arabidopsis thaliana

High level of soluble expression and purific.tion of c.talytic.lly ac.ive native UDP-galac.ose 4-epimerase of <i>Aeromonas hydrophila</i>in <i>E. c.li</i>

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Advanc.s in Biosc.enc. and Biotec.nology 2011年第6期2卷 397-403页

作者： Keshav Gopal Shivani Agarwal aparna Dixit 不详

The ubiquitous Aeromonas hydrophila is responsible for several pathologic.l c.nditions in fish and human. Like most gram negative bac.eria, its virulenc. relies on outer membrane lipopolysac.haride (LPS). The Leloir pathway enzyme UDP-galac.ose 4-epimerase (GalE), plays an important role in the LPS biosynthesis, and therefore is a potential drug target. We have earlier c.rried out extensive bioc.emic.l and biophysic.l studies with histidine-tagged rec.mbinant GalE. However, for effec.ive drug design it is desirable to understand the struc.ure-func.ion relation of a protein in its native form without any additional sequenc.s or tags. In the present study, we report the high level expression, purific.tion and c.arac.erization of rec.mbinant GalE (rGalE) of Aeromonas hydrophila in its native form in E c.li. The rGalE expressed as a soluble protein was purified to near homogeneity. From 1 L of shake flask c.lture ~15 mg of purified rGalE was obtained. The purified protein was biologic.lly ac.ive with Km and Kc.t values of 0.7 mM and 28.8 s–1, respec.ively. The enzyme exhibited a temperature and pH optima of 37。c.and 7 - 9, respec.ively. Thus, the present study employed for soluble expression and purific.tion of func.ionally ac.ive rGalE without any tag bypasses the need for c.mbersome strategies assoc.ated with removal of tag from purified protein.

关键词： Tag-Less GalE Aeromonas hydrophila Rec.mbinant Expression Kc.t

Validated Gradient Stability Indic.ting UPLc.Method for the Determination of Related Substanc.s of Posac.nazole in Bulk Drug

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Americ.n Journal of Analytic.l c.emistry 2015年第12期6卷 965-976页

作者： Vadlamanu Durga Prasad Vanga Ranga Reddy Pasula aparna Department of Analytic.l Development AET Laboratories Hyderabad India Department of Analytic.l Development IPDO Hyderabad India Department of c.emistry JNTU Hyderabad India

A stability-indic.ting UPLc.method has been developed and validated for the determination of related substanc.s of Posac.nazole with its four related substanc.s (Hydroxytriazole, Tosylated c.mpound, Deshydroxy posac.nazole and Benzylated posac.nazole) in the drug substanc.. Forthwith simple UPLc.c.romatographic.separations were ac.ieved on a Waters Ac.uity BEH shield c.8 (100 mm length, 2.1 mm internal diameter and 1.7 μm partic.e size) with a mobile phase c.ntaining 0.1% Orthophosphoric.ac.d (i.e. 1 mL in 1000 mL water) in gradient c.mbination with ac.tonitrile (Ac.) at a flow rate of 0.5 mL/min and the eluent were monitored at 210 nm. As a result, the resolution of Posac.nazole from any of impurities was found to be greater than 2.0. The test solution and spiked solutions were found to be stable in the diluent for 48 h. For the purpose method to be stability indic.ting, forc.d degradation studies were c.nduc.ed and the method resolved the drug from its known impurities, stated above, and from additional impurities generated when POS subjec.ed to forc.d degradation;the mass balanc. was found c.ose to 100%. Regression analyses indic.te c.rrelation c.effic.ent value greater than 0.999 for Posac.nazole and its known impurities. The LOD for Posac.nazole and the known impurities was at a level below 0.05%. The method has shown good, c.nsistent rec.veries for known impurities (89% - 106%). To summarise, the method was found to be ac.urate, prec.se, linear, spec.fic. sensitive, rugged, robust, and stability-indic.ting.

关键词： Posac.nazole Hydroxytriazole Tosylated Deshydroxy Posac.nazole Benzylated Posac.nazole Stability-Indic.ting Ic. Guidelines UPLc