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Variation of Bioactive compounds in Hypericum perforatum Growing in Turkey During Its Phenological cycle

Journal of Integrative Plant Biology 2007年第5期49卷 615-620页

作者： cüneyt cirak Jolita Radusiěne Birsen (Saglam) Karabük Valdimaras Janulis Liudas Ivanauskas Faculty of Agriculture Department of Agronomy University of Ondokuz Mayis Samsun 55139 Turkey Institute of Botany Zaliuju ezeru Vilnius LT-08406 Lithuania Kaunas University of Medicine Kaunas LT-44307 Lithuania

The present study was conducted to determine phenologic and morphogeneUc variation of hyperlcln, chlorogenlc acid and flavonoids, as rutin, hyperoside, apigenin-7-O-glucoside, quercitrin, quercetin content of Hypericum perforatum L. growing in Turkey. Wild growing plants were harvested at vegetative, floral budding, full flowering, fresh frulUng and mature fruiting stages and dissected into stem, leaf and reproductive tissues and assayed for bioacUve compounds by the High performance liquid chromatography （HPLc） method. Hypericin concentration ranged between 0 and 2.73 mg/g DW, chlorogenic acid 0.00-3.64 mg/g DW, rutin 0.00-3.36 mg/g DW, hyperoside 0.04- 22.42 mg/g DW, quercitrin 0.03-3.46 mg/g DW and quercetin 0.04-1.02 mg/g DW depending on ontogenetic and morphogenetic sampling. Leaves were found to be superior to stems and reproductive parts with regard to phenolic accumulation for all compounds tested while flowers accumulated the highest levels of hypericln. Quercltrln, quercetln and hypericin content in all tissues increased with advancing of developmental stages and reached their highest level during flower ontogenesis. Similarly, chlorogenic acid, hyperoside and apigenin-7-O-glucoside content in different plant parts increased during plant development, however, the highest level was observed at different stages of plant phenology for each tissue. chlorogenic acid was not detected in stems, leaves and reproductive parts in several stages of plant phenology and its variation during plant growth showed inconsistent manner. In contrast to the other compounds examined, rutin content of stems and leaves decreased with advanc- ing of plant development and the highest level for both tissues was observed at the vegetative stage. However, content of the same compound in reproductive parts was the highest at mature fruiting. The present findings might be useful to obtain increased concentration of these natural compounds.

关键词： chiorogenic acid flavonoids hypericin Hypericum perforatum morphogenetic variation phenologic variation.

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computational Studies on the Ground State Tautomer,Hydrogen conformations and Vibrational Spectroscopic Analysis of Antitumor Agents:3-Deazauracil and 6-Azauracil

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光谱学与光谱分析 2018年第4期38卷 1276-1282页

作者： cagri cirak Nurettin Korozlu Yusuf Sert Fatih Ucun Department of Physics Faculty of Arts and SciencesErzincan UniversityErzincanTurkey Department of Nanoscience and Nanotechnology Faculty of Arts and SciencesMehmet Akif Ersoy UniversityBurdurTurkey Department of Physics Faculty of Arts and SciencesBozok UniversityYozgatTurkey Department of Physics Faculty of Arts and SciencesSüleyman DemirelUniversityIspartaTurkey

The ground state hydrogen conformations and vibrational analysis of 3-deazauracil(3DAU)and 6-azauracil(6AU)tautomers(4-enol and 2,4-diol forms)have been calculated using ab initio Hartree-Fock(HF)and density functional theory(B3LYP)methods with 6-311++G(d,p)basis set *** calculations have shown that the most probably preferential tautomer of 3DAU and 6AU are the 4-enol form,which gives best fit to the corresponding experimental *** ground state conformer of the 2,4-diol form has two O—H bonds which are oriented externally and internally(to the N—H bond).The vibrational analyses of the ground state conformer of each tautomeric form of 3DAU and 6AU were done and their optimized geometry parameters(bond lengths and bond angles)were ***,from the correlations values it was concluded that the B3LYP method is superior to the HF method for both the vibrational frequencies and the geometric parameters.

关键词： 3-Deazauracil 6-Azauracil Tautomerism conformation Vibrational spectra

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