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Effects of lead(Pb)-induced oxidative stress on morphological and physio-biochemical properties of rice

BIOCELL 2021年第5期45卷 1413-1423页

作者： MURTAZA KHAN IBA NAZAR IBRAHIM AL AZZAWI MUHAMMAD IMRAN ADIL HUSSAIN BONG-GYU MUN ANJALI PANDE byung-wook yun Laboratory of Plant Functional Genomics School of Applied BiosciencesKyungpook National UniversityDaegu41566South Korea Ministry of Agriculture State Company for Agriculture Supplies Baghdad10064Iraq Laboratory of Crop Physiology School of Applied BiosciencesKyungpook National UniversityDaegu41566South Korea Department of Entomology Abdul Wali Khan University MardanKhyber Pakhtunkhwa23200Pakistan

In rice,high concentration of lead(Pb)can cause phyto-toxicity affecting several physiological *** of rice varieties that are resistant to Pb-induced oxidative stress is an important management strategy in Pb-contaminated *** the current study,we evaluated four different rice cultivars for their response to Pb-induced *** japonica type cultivars X-Jigna,Ediget,and Furat,and one Indica type cultivar Amber 33 were grown in soil containing different Pb concentrations(0 mM,0.6 mM,and 1.2 mM).The soil was treated with 0 mM or 0.6 mM or 1.2 mM Pb solution one month prior to rice seedling ***,four-week-old rice seedlings were transplanted into the treated soil and their responses were observed until *** data revealed that a highest concentration of Pb(1.2 mM)induced significant reduction in agronomic traits such as plant height,number of tillers per plant,number of panicles per plant,and number of spikelets per panicle in all the rice ***,least reduction in the agronomic traits was observed in X-Jigna,whereas the highest reduction in the agronomic traits was observed in *** activity of catalase(CAT),peroxidase(POD),polyphenol oxidase(PPO),and superoxide dismutase(SOD),was evaluated along with the accumulation of superoxide ions(O2.-),protein,proline,chlorophyll,sucrose,glucose,and fructose contents in all the rice cultivars.A significant increase in antioxidant activity and in the accumulation of proline and sucrose contents with the least reduction in the chlorophyll and protein contents was observed in X-Jigna suggesting that X-Jigna is the most tolerant among all the rice cultivars tested against *** the other hand,non-significant and slightly significant increase in the antioxidant activity,less accumulation of proline and sucrose contents,and higher reduction in the chlorophyll and protein contents was observed in Ediget,which further suggest that Ediget is the most s

关键词： Lead(Pb)toxicity Oxidative stress Morphological and physio-biochemical properties of rice

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Analysis of Rice Grain Quality-Associated Quantitative Trait Loci by Using Genetic Mapping

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American Journal of Plant Sciences 2014年第9期5卷 1125-1132页

作者： byung-wook yun Min-Gyu Kim Tri Handoyo Kyung-Min Kim Division of Plant Biosciences School of Applied Biosciences College of Agriculture & Life Science Kyungpook National University Daegu South Korea

The main objective of this research was to identify quantitative trait loci associated with rice qualities to provide reliable information for marker-assisted selection and development of new varieties. In total, 120 doubled haploid (DH) lines developed by another culture from the F1 hybrid of a cross between “Cheongcheong”, a Tongil variety, and “Nagdong”, a japonica variety, were used. A microsatellite linkage map of 222 markers spanned 2082.4 centimorgans (cM) and covered 12 rice chromosomes with an average interval of 9.4cM between markers. Eight quantitative trait loci (QTLs) were associated with rice quality, consisting of two QTLs on chromosomes 1 and 9 for amylose content;three QTLs on chromosomes 8, 9, and 10 for protein content;and three QTLs on chromosomes 2, 3, and 6 for lipid content. PCR expression levels measured using the SSR markers RM23914 for proteins and RM6266 for lipids, and RM586 showed a higher degree of amplification. The present study should be useful for improving the nutritional quality of rice by means of marker-assisted selection.

关键词： Grain Quality QTL Rice Genetic Map Doubled Haploid

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Redox sensor QSOX1 regulates plant immunity by targeting GSNOR to modulate ROS generation

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Molecular Plant 2021年第8期14卷 1312-1327页

作者： Ho Byoung Chae Min Gab Kim Chang Ho Kang Joung Hun Park Eun Seon Lee Sang-Uk Lee Yong Hun Chi Seol Ki Paeng Su Bin Bae Seong Dong Wi byung-wook yun Woe-Yeon Kim Dae-Jin yun David Mackey Sang Yeol Lee Division of Applied Life Sciences(BK21)and PMBBRC Gyeongsang National UniversityJinju 52828Korea College of Pharmacy Research Institute of Pharmaceutical ScienceGyeongsang National UniversityJinju 52828Korea School of Applied Biosciences College of Agriculture and Life SciencesKyungpook National UniversityDaegu 41566Korea Department of Biomedical Science and Engineering Konkuk UniversitySeoul 05029Korea Department of Horticulture and Crop Science Department of Molecular Geneticsand Center for Applied Plant SciencesThe Ohio State UniversityColumbusOH 43210USA College of Life Sciences Qingdao Agricultural UniversityQingdao 266109P.R.China Plant Propagation Team Plant Production DivisionSejong National ArboretumSejong 30106Korea

Reactive oxygen signaling regulates numerous biological processes,including stress responses in *** sensors transduce reactive oxygen signals into cellular ***,we present biochemical evidence that a plant quiescin sulfhydryl oxidase homolog(QSOX1)is a redox sensor that negatively regulates plant immunity against a bacterial *** expression level of QSOX1 is inversely correlated with pathogen-induced reactive oxygen species(ROS)***,QSOX1 both senses and regulates ROS levels by interactingn with and mediating redox regulation of S-nitrosoglutathione reductase,which,consistent with previous findings,influences reactive nitrogen-mediated regulation of ROS ***,our data indicate that QSOX1 is a redox sensorthat negatively regulates plant immunity by linking reactive oxygen and reactive nitrogen signaling to limit ROS production.

关键词： redox sensor plant immu nity QSOX1 GSNOR reactive oxygen species(ROS) reactive nitrogen species(RNS)

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Analysis of High-Resolution QTL Markers Associated with Rice Yields Using Data for Two Consecutive Years in Different Environmental Conditions

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Natural Science 2014年第11期6卷 818-827页

作者： Ye-Jin Son Gyu-Ho Lee Hyun-Suk Lee Tri Handoyo byung-wook yun Kyung-Min Kim Division of Plant Biosciences School of Plant Biosciences Kyungpook National University Daegu Korea Division of Plant Biosciences School of Plant Biosciences Kyungpook National University Daegu Korea Department of Agronomy Faculty of Agriculture Jember University Jember Indonesia

Previously we reported the identification of seven quantitative trait loci (QTLs) associated with the rice yield measuring five parameters including panicles per plant (PPP), spikelets per panicle (SPP), seed set percentage (SSP), 1000-grain weight (TGW) and yield in 2012. Here we report the analysis of QTLs using the same trait parameters data of the mapping population in 2013 for detecting highly conserved QTL markers. A total of 6 QTLs were identified from chromosomes 1, 7, 8, 10, 11, and 12, which were contrasted with our previous results (chromosomes 1, 2, 4, 5, 6, 8, and 11). In this comparison, three QTLs from chromosome 1, 8, and 11 were only found to be associated with the components of yield over two consecutive years indicating high sensitivity of QTL markers to the environment. Of those three QTLs, SPP-associated marker RM12285 was found to be dominantly expressed by real-time PCR (qPCR). In addition, compared to our previous report the numbers of mapping population and markers were significantly increased for higher resolution markers from 70 to 120, and from 143 to 217, respectively. We also found that the parameter SPP was dominantly correlated with the rice yield. Furthermore, the double haploid (DH) population facilitated to analyze the epistatic effects for yield and yield components in rice. Taken together, combining multiple mapping population data over years possibly enables narrowing down to the highly conserved QTL markers against diverse environmental fluctuation caused by such as drought and high temperature. Thus, these data would be critically exploited to improve for the crop breeding strategy.

关键词： QTL Rice Yield Component Epistatic Interaction qPCR

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Ethyl acetate fraction of Sargassum pallidum extract attenuates particulate matter-induced oxidative stress and inflammation in keratinocytes and zebrafish

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Asian Pacific Journal of Tropical Biomedicine 2024年第4期14卷 137-146页

作者： wook Chul Kim Ji-Won Park Bohyun yun WonWoo Lee Kyung-Min Choi Seung-Hong Lee Department of Medical Science Soonchunhyang UniversityAsan 31538Republic of Korea Honam National Institute of Biological Resources 99Gohadoan-gilMokpo-siJeollanam-do 58762Republic of Korea Department of Pharmaceutical Engineering Soonchunhyang UniversityAsan 31538Republic of Korea

Objective:To evaluate the effect of the ethyl acetate fraction derived from Sargassum pallidum extract against particulate matter(PM)-induced oxidative stress and inflammation in HaCaT cells and ***:HaCaT cells and zebrafish were used to evaluate the protective effects of the ethyl acetate fraction of Sargassum pallidum extract against PM-induced oxidative stress and *** production of nitric oxide(NO),intracellular ROS,prostaglandin E_(2)(PGE_(2)),and pro-inflammatory cytokines,and the expression levels of COX-2,iNOS,and NF-κB were evaluated in PM-induced HaCaT ***,the levels of ROS,NO,and lipid peroxidation were assessed in the PM-exposed zebrafish ***:The ethyl acetate fraction of Sargassum pallidum extract significantly decreased the production of NO,intracellular ROS,and PGE_(2) in PM-induced HaCaT *** addition,the fraction markedly suppressed the levels of pro-inflammatory cytokines and inhibited the expression levels of COX-2,iNOS,and NF-κ***,it displayed remarkable protective effects against PM-induced inflammatory response and oxidative stress,represented by the reduction of NO,ROS,and lipid peroxidation in ***:The ethyl acetate fraction of Sargassum pallidum extract exhibits a protective effect against PM-induced oxidative stress and inflammation both in vitro and in vivo and has the potential as a candidate for the development of pharmaceutical and cosmeceutical products.

关键词： Particulate matter Inflammation Oxidative stress Sargassum pallidum Ethyl acetate fraction Zebrafish

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Highly Aligned Ternary Nanofiber Matrices Loaded with MXene Expedite Regeneration of Volumetric Muscle Loss

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Nano-Micro Letters 2024年第4期16卷 269-292页

作者： Moon Sung Kang Yeuni Yu Rowoon Park Hye Jin Heo Seok Hyun Lee Suck Won Hong yun Hak Kim Dong‑wook Han Department of Cogno‑Mechatronics Engineering College of Nanoscience and NanotechnologyPusan National UniversityBusan 46241Republic of Korea Medical Research Institute School of MedicinePusan National UniversityYangsan 50612Republic of Korea Department of Anatomy School of MedicinePusan National UniversityYangsan 50612Republic of Korea Engineering Research Center for Color‑Modulated Extra‑Sensory Perception Technology Pusan National UniversityBusan 46241Republic of Korea Department of Biomedical Informatics School of MedicinePusan National UniversityYangsan 50612Republic of Korea Periodontal Disease Signaling Network Research Center and Dental and Life Science Institute School of DentistryPusan National UniversityYangsan 50612Republic of Korea BIO‑IT Fusion Technology Research Institute Pusan National UniversityBusan 46241Republic of Korea Present Address:Osstem Implant Inc. Seoul 07789Republic of Korea

Current therapeutic approaches for volumetric muscle loss(VML)face challenges due to limited graft availability and insufficient *** overcome these limitations,tissue-engineered scaffolds have emerged as a promising *** this study,we developed aligned ternary nanofibrous matrices comprised of poly(lactide-co-ε-caprolactone)integrated with collagen and Ti_(3)C_(2)T_(x)MXene nanoparticles(NPs)(PCM matrices),and explored their myogenic potential for skeletal muscle tissue *** PCM matrices demonstrated favorable physicochemical properties,including structural uniformity,alignment,microporosity,and *** vitro assays revealed that the PCM matrices promoted cellular behaviors and myogenic differentiation of C2C12 ***,in vivo experiments demonstrated enhanced muscle remodeling and recovery in mice treated with PCM matrices following VML *** insights from next-generation sequencing revealed that MXene NPs facilitated protein and ion availability within PCM matrices,leading to elevated intracellular Ca^(2+)levels in myoblasts through the activation of inducible nitric oxide synthase(i NOS)and serum/glucocorticoid regulated kinase 1(SGK1),ultimately promoting myogenic differentiation via the m TOR-AKT ***,upregulated i NOS and increased NO–contributed to myoblast proliferation and fiber fusion,thereby facilitating overall myoblast *** findings underscore the potential of MXene NPs loaded within highly aligned matrices as therapeutic agents to promote skeletal muscle tissue recovery.

关键词： Ti_(3)C_(2)T_(x)MXene nanoparticle Ternary nanofibrous matrices Myogenesis Regeneration of volumetric muscle loss Next generation sequencing

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Vehicle Positioning Based on Optical Camera Communication in V2I Environments

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Computers, Materials & Continua 2022年第8期72卷 2927-2945页

作者： Pankaj Singh Huijin Jeon Sookeun yun byung wook Kim Sung-Yoon Jung Department of Electronic Engineering Yeungnam UniversityGyeongsan38541Korea HD Map Development Team Hyundai Autoever Co.Ltd.Seoul06171Korea Department of Information and Communication Engineering Changwon National UniversityChangwon51140Korea

Demand for precise vehicle positioning(VP)increases as autonomous vehicles have recently been drawing *** paper proposes a scheme for positioning vehicles on the move based on optical camera communication(OCC)technology in the vehicle-to-infrastructure(V2I)***-emitting diode(LED)streetlights and vehicle cameras are used as transmitters and receivers *** of streetlights are detected and traced by examining images that are obtained from cameras of ***,a scheme for analyzing visible light data extracted from the images is *** proposed vehicle positioning scheme uses information on angles between vectors that are formed under the collinearity conditions between the absolute coordinates of at least three received streetlights,and the coordinates of an image *** experiments are performed under stationary state and moving state at a speed of 5 and 20 km/*** verify the reliability of the proposed scheme,a comparison is made between the actual and estimated location of the camera in the stationary *** addition,the path of a moving vehicle and the estimated path of the vehicle are compared to check the performance of the *** performance of the proposed technique is analyzed and experimental demonstration confirms that the proposed OCC-based VP scheme achieves positioning accuracy of under 1 m.

关键词： Optical camera communication vehicle-to-infrastructure LED streetlight intelligent transport system vehicle positioning collinearity

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Tunnel flexibility effect on the ground surface acceleration response

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Earthquake Engineering and Engineering Vibration 2016年第3期15卷 457-476页

作者： Mohammad Hassan Baziar asoud Rabeti Moghadam yun wook Choo Dong-Soo Kim Center of Excellence for Fundamental Studies in Structural Engineering School of Civil EngineeringIran University of Science and TechnologyTehranIran School of Civil Engineering Iran University of Science and TechnologyTehran1ran Department of Civil and Environmental Engineering College of EngineeringKongju National UniversityChungnam 330-717Republic of Korea Department of Civil and Environmental Engineering Korea Advanced Institute of Science&TechnologyDaejeon 305-701Republic of Korea

Flexibility of underground structures relative to the surrounding medium, referred to as the flexibility ratio, is an important factor that influences their dynamic interaction. This study investigates the flexibility effect of a box-shaped subway tunnel, resting directly on bedrock, on the ground surface acceleration response using a numerical model verified against dynamic centrifuge test results. A comparison of the ground surface acceleration response for tunnel models with different flexibility ratios revealed that the tunnels with different flexibility ratios influence the acceleration response at the ground surface in different ways. Tunnels with lower flexibility ratios have higher acceleration responses at short periods, whereas tunnels with higher flexibility ratios have higher acceleration responses at longer periods. The effect of the flexibility ratio on ground surface acceleration is more prominent in the high range of frequencies. Furthermore, as the flexibility ratio of the tunnel system increases, the acceleration response moves away from the free field response and shifts towards the longer periods. Therefore, the flexibility ratio of the underground tunnels influences the peak ground acceleration （PGA） at the ground surface, and may need to be considered in the seismic zonation of urban areas.

关键词： box-shaped tunnel flexibility ratio acceleration response ground surface centrifuge modeling numericalsimulation

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Hybrid Friction Stir Welding of High-carbon Steel

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Journal of Materials Science & Technology 2011年第2期27卷 127-130页

作者： Don-Hyun Choi Chang-Yong Lee byung-wook Ahn Jung-Hyun Choi yun-Mo Yeon Keun Song Seung-Gab Hong Won-Bae Lee Ki-Bong Kang Seung-Boo Jung School of Advanced Materials Science and Engineering Sungkyunkwan University 300 Cheoncheon-dongJangan-guSuwonGyeonggi-do 440-746South Korea Department of Mechanical Engineering Korea Advanced Institute of Science and Technology 335 GwahangnoYuseong-guDaejeon 305-701South Korea Department of Advanced Materials Application Suwon Science College 9-10Botong-liJeongnam-myeonHwasungGyeonggi-do 445-742South Korea Technical Research Lab POSCO Corp.Goedong-dongNam-guPohangGyeongbuk790-785South Korea

A high-carbon steel joint,SK5（0.84 wt% C）,was successfully welded by friction stir welding（FSW）,both without and with a gas torch,in order to control the cooling rate during *** welding,the weld zone comprised gray and black regions,corresponding to microstructural variation：a martensite structure and a duplex structure of ferrite and cementite,*** volume fraction of the martensite structure and the Vickers hardness in the welds were decreased with the using of the gas torch,which was related with the lower cooling rate.

关键词： Friction stir welding High-carbon steel Transformation Martensite

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Association of chronic viral hepatitis B with insulin resistance

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World Journal of Gastroenterology 2012年第42期18卷 6120-6126页

作者： Jeong Gyu Lee Sangyeoup Lee yun Jin Kim byung Mann Cho Joo Sung Park Hyung Hoi Kim JaeHun Cheong Dong wook Jeong Yu Hyun Lee Young Hye Cho Mi Jin Bae Eun Jung Choi Department of Family Medicine Pusan National University School of MedicineBeomeo-ri Mulgeum-eupYangsan 626-770South Korea Department of Family Medicine Medical Research InstitutePusan National University HospitalBusan 602-739South Korea Medical Education Unit Pusan National University School of MedicineBeomeo-ri Mulgeum-eupYangsan 626-770South Korea Family Medicine Clinic Yangsan Pusan National University HospitalBeomeo-ri Mulgeum-eupYangsan 626-770South Korea Department of Preventive Medicine and Occupational Medicine Pusan National University School of MedicineBeomeo-ri Mulgeum-eupYangsan 626-770South Korea Department of Family Medicine Dong-A University Medical CenterBusan 602-715South Korea Department of Laboratory Medicine Biomedical Informatics UnitPusan National University School of MedicineBeomeo-ri Mulgeum-eupYangsan 626-770South Korea Department of Molecular Biology College of Natural SciencesPusan National UniversityBusan 602-739South Korea

AIM:To investigate the relationship between chronic viral hepatitis B(CVHB) and insulin resistance(IR) in Korean ***:A total of 7880 adults(3851 men,4029 women) who underwent a comprehensive medical examination were enrolled in this *** diagnosed with either diabetes mellitus,or any other disorder that could influence their insulin sensitivity,were ***,metabolic risk factors,hepatitis B surface antigen,hepatitis B surface antibody,hepatitis B core antibody,fasting plasma glucose and insulin were measured for all *** model assessment(HOMA),quantitative insulin check index(QUICKI),and Mf fm index were used for determining insulin *** participant was categorized into a negative,recovery,or CVHB *** compare variables between groups,a t-test and/or one-way analysis of variance were *** correlation coefficients were computed to present the association between insulin resistance and other *** logistic regression analysis was used to assess the independent association between CVHB and ***:The mean age of men and women were 48.9 and 48.6 years,*** in the CVHB group had significantly higher waist circumference [(86.0 ± 7.7 cm vs 87.3 ± 7.8 cm,P = 0.004 in men),(78.3 ± 8.6 cm vs 80.5 ± 8.5 cm,P < 0.001 in women)],cystatin C [(0.96 ± 0.15 mg/dL vs 1.02 ± 0.22 mg/dL,P < 0.001 in men),(0.84 ± 0.15 mg/dL vs 0.90 ± 0.16 mg/dL,P < 0.001 in women)],fasting insulin [(5.47 ± 3.38 U/mL vs 6.12 ± 4.62 U/mL,P < 0.001 in men),(4.57 ± 2.82 U/mL vs 5.06 ± 3.10 U/mL,P < 0.001 in women)] and HOMA index [(1.24 ± 0.86 vs 1.43 ± 1.24,P < 0.001 in men),(1.02 ± 0.76 vs 1.13 ± 0.87,P = 0.033 in women)] compared to control *** HOMA index revealed a positive correlation with body mass index(BMI)(r = 0.378,P < 0.001),waist circumference(r =0.356,P < 0.001),percent body fat(r = 0.296,P < 0.001),systolic blood pressure(r = 0.202,P < 0.001),total cholesterol(r = 0.134,P < 0.00

关键词： Hepatitis B Insulin resistance Diabetes mellitus type 2 Metabolic syndrome

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