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Phytochemical analysis of Berberis lyceum methanolic extract and its antiviral activity through the restoration of MAPK signaling pathway modulated by HCV NS5A

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Asian Pacific Journal of Tropical Biomedicine 2021年第3期11卷 132-140页

作者： Koloko Brice Landry Shafiq Azam Sidra Rehman Somayya Tariq bushra Iqbal Mateen Abbas Dieudonné Massoma Lembè bushra ijaz Laboratory of Applied and Functional Genomic Centre of Excellence in Molecular BiologyUniversity of the PunjabLahorePakistan Department of Biosciences COMSATS University IslamabadIslamabadPakistan Quality Operations Laboratory University of Veterinary and Animal SciencesLahorePakistan Laboratory of Animal Biology and Physiology Faculty of ScienceUniversity of DoualaDoualaCameroon

Objective:To evaluate the antiviral activity and phytochemicals of selected plant extracts and their effect on the mitogen-activated protein kinase(MAPK)signaling pathway modulated by hepatitis C virus(HCV)nonstructural protein 5 A(NS5A).Methods:A total of ten plant extracts were initially screened for their toxicities against Hep G2 *** non-toxic plants were tested for their inhibitory effect on the expression of HCV NS5A at both m RNA and protein levels using real-time PCR and Western blotting assays,*** differential expression of the genes associated with MAPK pathway in the presence of NS5A gene and plant extract was measured through real-time ***,the identification of secondary metabolites was carried out by phytochemical and HPLC ***:The phytochemical profiling of Berberis lyceum revealed the presence of alkaloids,phenols,saponins,tannins,flavonoids,carbohydrates,terpenoids,steroids,and ***,quercetin,myricetin,gallic acid,caffeic acid,and ferulic acid were identified through HPLC *** methanolic extract of Berberis lyceum strongly inhibited HCV RNA replication with an IC50 of 11.44μg/m ***-PCR and Western blotting assays showed that the extract reduced the expression of HCV NS5A in a dosedependent *** lyceum extract also attenuated NS5A-induced dysregulation of the MAPK signaling ***:Our findings suggest that Berberis lyceum extract strongly inhibits HCV propagation by reducing HCV NS5A-induced perturbation of MAPK signaling.

关键词： Anti-HCV HCV NS5A Berberis lyceum MAPK pathway Phytochemicals HPLC

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HCV infection causes cirrhosis in human by step-wise regulation of host genes involved in cellular functioning and defense during fibrosis:Identification of bio-markers

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Genes & Diseases 2019年第3期6卷 304-317页

作者： bushra ijaz Waqar Ahmad Trina Das Khadija Shabbiri Tayyab Husnain Sajida Hassan Centre of Excellence in Molecular Biology University of the PunjabLahorePakistan School of Biological Sciences The University of QueenslandAustralia College of Medicine and Health Sciences UAE UniversityAl AinUnited Arab Emirates Division of Transplantation Department of SurgerySchool of MedicineUniversity of WashingtonSeattleWAUSA Department of Laboratory Medicine University of WashingtonSeattleWAUSA

Chronic Hepatitis C Viral(HCV)infection is a leading health problem worldwide and resulted in fibrotic scar formation,and finally *** contemporary therapies can partially reverse this destructive process,the rehabilitation is too slow and unsuitable for all chronic *** current study elucidates the mechanism of disease progression from early(F1)to moderate(F2,F3),and to severe fibrosis(F4)/cirrhosis in HCV genotype 3a infected patients to find out new candidates as potential disease progression markers and antiviral therapeutic agents.A total of 550 genes were found differentially regulated in the four fibrosis stages and grouped in 22 classes according to their biological *** set enrichment(GSEA)and Ingenuity pathway analysis(IPA)were used to identify the regulation of crucial biological functions and pathways involved in HCV *** differentially regulated the expression of genes involved in apoptosis,cell structure,signal transduction,proliferation,metabolism,cytokine signaling,immune response,cell adhesion and maintenance,and post translational modifications by pathway *** was an increasing trend of proliferative and cell growth related genes and shutting down of immune response as the disease progress mild to moderate to advanced stage *** myriad of changes in gene expression showed more chances of developing liver cancer in patients infected with HCV genotype 3a in a systematic *** identified gene set can act as disease markers for prediction,whether the fibrosis lead to cirrhosis and its association with end stage liver disease development.

关键词： Gene expression profiling Hepatitis C Liver cirrhosis Liver fibrosis Viral infection

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HCV genotype distribution and possible transmission risks in Lahore, Pakistan

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World Journal of Gastroenterology 2010年第34期16卷 4321-4328页

作者： Waqar Ahmad bushra ijaz Fouzia Tahir Javed Shah Jahan Imran Shahid Fawad Mumtaz Khan Sajida Hassan Applied and Functional Genomics Lab Centre of Excellence in Molecular Biology University of the Punjab Applied and Functional Genomics Lab Centre of Excellence in Molecular Biology University of the Punja Department of Pathology Jinnah Hospital Plastic Surgery Department Mayo Hospital

AIM: To investigate the prevalence of hepatitis C virus (HCV) genotypes and their association with possible transmission routes in the general population of Lahore, as the data exclusively related to this city is limited. METHODS: Complete data regarding patient's history, possible route of infection and biochemical tests was collected from the public hospital for 1364 patients. SPSS version 16 windows software was used for data analysis by univariate and multivariate techniques. RESULTS: Age range ≤ 40 years showed high prevalence of HCV infection. HCV genotype 3a was dominant (55.9%), followed by 1a (23.6%), 4a (12.5%), 3b (3.2%), untypable (2.5%), 4b (1.2%) and mixed type (1.2%). Blood transfusion, dental surgery and barber shops were the main risk factors for HCV transmission. Genotype prevalence was independent of age (P = 0.971) and gender (P = 0.122) while risk factors showed a significant association with age (P = 0.000) and genotypes (P = 0.000). We observed an independent association of risk factors and genotype 3a, while patients with genotype 1 and 4 were mostly infected due to dental surgery blood transfusion and barber shops. Risk factors of intravenous drug use and sexual exposure were exclusively found in ≤ 40 years age group. CONCLUSION: An increase in genotypes 1a and 4a suggest migration of people, possibly from Balochistan and the northern war-zone area. Government should focus on public education regarding infection routes.

关键词： Hepatitis C virus Prevalence Genotypes Risk factors Lahore

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