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Barley chitinase genes expression revamp resistance against whitefly (Bemisia Tabaci) in transgenic cotton (Gossypium hirsutum L.)

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Journal of Cotton Research 2024年第1期7卷 90-100页

作者： BASHIR Samina YAQOOB Amina BASHIR Rohina bukhari shanila SHAHID Naila AZAM Saira BAKHSH Allah HUSNAIN Tayyab SHAHID Ahmad Ali RAO Abdul Qayyum Centre of Excellence in Molecular Biology University of the PunjabLahore 53700Pakistan Department of Botany Kinnaird College for Women UniversityLahore 54000Pakistan Department of Botany Government College UniversityFaisalabad 38000Pakistan Institute of Botany University of the PunjabLahore 54590Pakistan.

Background Chitinase is an enzyme that hydrolyzes chitin,a major component of the exoskeleton of insects,including plant pests like *** present study aimed to investigate the expression of chemically synthesized barley ch1 and chi2 genes in cotton(Gossypium hirsutum)through Agrobacterium-mediated ***-five putative transgenic cotton plants were obtained,out of which fifteen plants successfully survived and were shifted to the *** gene-specific primers,amplification of 447 bp and 401 bp fragments confirmed the presence of the ch1 and chi2 genes in five transgenic cotton plants of the T0 *** five plants were further evalu-ated for their mRNA expression *** T0 transgenic cotton plants with the highest mRNA expression level and better yield performance in field,were selected to raise their subsequent *** The T1 cotton plants showed the highest mRNA expression levels of 3.5-fold in P10(2)for the ch1 gene and 3.7-fold in P2(1)for the chi2 *** in situ hybridization(FISH)confirmed a single copy number of ch1 and chi2(hemizygous)on chromosome ***,the efficacy of transgenes on whitefly was evaluated through an insect bioassay,where after 96 h of infestation,mortality rates of whitefly were calculated to be 78%–80%in transgenic cotton *** number of eggs on transgenic cotton plants were calculated to be 0.1%–0.12 per plant compared with the non-transgenic plants where egg number was calculated to be 0.90–1.00 per *** Based on these findings,it can be concluded that the chemically synthesized barley chitinase genes(ch1 and chi2)have the potential to be effective against insects with chitin exoskeletons,including *** transgenic cotton plants expressing these genes showed increased resistance to whiteflies,resulting in reduced egg numbers and higher mortality rates.

关键词： Chitinase Cotton White fly Transgene Bioassay

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Barley chitinase genes expression revamp resistance against whitefly (BemisiaTabaci ) in transgenic cotton ( L.)

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棉花研究(英文) 2024年第1期 90页

作者： BASHIR Samina YAQOOB Amina BASHIR Rohina bukhari shanila SHAHID Naila AZAM Saira BAKHSH Allah HUSNAIN Tayyab SHAHID Ahmad Ali RAO Abdul Qayyum

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PRPS:用于移动群智感知的隐私保护和信誉感知的参与者选择方案

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Journal of Donghua University(English Edition) 2024年第2期41卷 195-205页

作者： AZHAR shanila 刘国华东华大学计算机科学与技术学院上海201620

作为一种新兴的感知范式,移动群智感知(mobile crowd sensing,MCS)包括一组移动用户,这些用户利用他们的传感设备有效地执行和发送数据贡献。然而,隐私和信誉机制(可靠性评估)的集成是构建安全可靠的MCS应用程序的关键。首先,即使参与... 详细信息

作为一种新兴的感知范式,移动群智感知(mobile crowd sensing,MCS)包括一组移动用户,这些用户利用他们的传感设备有效地执行和发送数据贡献。然而,隐私和信誉机制(可靠性评估)的集成是构建安全可靠的MCS应用程序的关键。首先,即使参与者提供敏感的个人数据,也能确保他们的隐私得到保护。其次,由于有偏见或不准确的贡献可能会降低系统质量,信誉机制允许服务器监控参与者的行为和可靠性,服务器必须对参与者进行验证。将信誉机制与隐私相结合具有挑战性和矛盾性。信誉机制衡量参与者在整个感知活动期间的行为,而隐私旨在保护参与者的身份。因此,提出了一种针对MCS的新型隐私保护和信誉感知的参与者选择(privacy-preserving and reputation-aware participant selection,PRPS)方案。PRPS方案将隐私与信誉机制相结合,使用假名和隐身技术来分别保护参与者身份和信誉值隐私,并保护位置和数据隐私。通过仿真模拟和性能评估,分别比较了PRPS方案、隐私保护和效用感知的参与者选择(privacy-preserving and utility-aware participant selection,PUPS)方案及效用感知的参与者选择(utilityaware participant selection,UPS)方案,证明了PRPS方案的精度、有效性和可扩展性,并证明了隐私和信誉机制对数据贡献的影响。再次,评估了PRPS方案的结果。最后,估计了PRPS方案在评估参与者可靠性和行为方面的效率和准确性。

关键词：移动群智感知(MCS) 信誉隐私假名隐身

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Alkaloids, flavonoids, polyphenols might be responsible for potent antiarthritic effect of Solanum nigrum

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Journal of Traditional Chinese Medicine 2019年第5期39卷 632-641页

作者： Alamgeer shanila Akhter Ambreen Malik Uttra Umme Habiba Hasan Laboratory of Cardiovascular Research and Integrative Pharmacology College of Pharmacy University of Sargodha

OBJECTIVE: To evaluate in vitro and in vivo antiarthritic potential of Solanum nigrum (S. nigrum). METHODS: Aqueous methanolic (70∶30) extract of S. nigrum was prepared. The in vitro antiarthritic effect was evaluated in terms of its inhibition of protein denaturation and membrane stabilization. While, formaldehyde, complete Freund's adjuvant (CFA) and Collagen induced arthritis rat models were used to study in vivo antiarthritic activities of S. nigrum at dose level of 200, 400 and 800 mg/kg. RESULTS: The extract exhibited inhibition of protein denaturation and protected red blood cell by stabilizing the membranes in a concentration dependent manner, with maximum effect attained at 800 μg/mL. Moreover, there was a marked reduction in paw edema observed in extract treated animals, when compared to arthritic control animals in all in vivo models and 800 mg/kg dose got maximum reduction of paw edema. In CFA and collagen models, plant extract restored body weight, hematologic parameters, radiographic and histopathoOBJECTIVE: To evaluate in vitro and in vivo antiarthritic potential of Solanum nigrum (S. nigrum). METHODS: Aqueous methanolic (70∶30) extract of S. nigrum was prepared. The in vitro antiarthritic effect was evaluated in terms of its inhibition of protein denaturation and membrane stabilization. While, formaldehyde, complete Freund's adjuvant (CFA) and Collagen induced arthritis rat models were used to study in vivo antiarthritic activities of S. nigrum at dose level of 200, 400 and 800 mg/kg. RESULTS: The extract exhibited inhibition of protein denaturation and protected red blood cell by stabilizing the membranes in a concentration dependent manner, with maximum effect attained at 800 μg/mL. Moreover, there was a marked reduction in paw edema observed in extract treated animals, when compared to arthritic control animals in all in vivo models and 800 mg/kg dose got maximum reduction of paw edema. In CFA and collagen models, plant extract restored body weigh

关键词： Arthritis Solanum nigrum Protein denaturation Formaldehyde Freund's adjuvant Collagen

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