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Subcutaneous Model for the Study of Dengue Virus Infection in Immune Competent Mice

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Journal of Biosciences and Medicines 2018年第5期6卷 97-110页

作者： Beatriz Senra Santos Natalia lima Pessoa Natalia Lucinda Gustavo Cardoso de Oliveira Thais Souza Silva Ketyllen Reis Andrade Ketyllen Reis Andrade bruno Galvã o Filho Marcele Neves Rocha Alexandre Vieira Machado Pedro Augusto Alves É rica Alessandra Rocha Alves Erna Geessien Kroon Marco Antô nio Campos Instituto René Rachou Funda&amp amp ccedil & amp atilde o Oswaldo Cruz Fiocruz Belo Horizonte Brazil Departamento de Microbiologia Universidade Federal de Minas Gerais Belo Horizonte Brazil Empresa Brasileira de Pesquisa Agropecuá ria Laborat&amp amp oacute rio de Virologia Brasilia Brazil

Various mouse models to study dengue have been described by different authors, some of them using immunodeficient or some using humanized mice. Our group reported previously a deadly murine model, which used the intracranial inoculum of highly virulent Dengue virus (DENV) in immune competent mouse. Here we present a model of immune competent mouse (C57BL/6), infected subcutaneously by the same highly virulent DENV (DENV3 genotype I). In this immunocompetent systemic mice model, the cytokine levels and hematological parameters such as total and differential leukocyte and platelets counts, together with weight loss, were considered important monitoring parameters, allowing a better understanding of the systemic human disease. Mice were inoculated subcutaneously and evaluated by the percentage weight variation as well as the clinical signs. Hematological parameters and cytokines levels were measured and viral titration in brain tissue or serum neutralization was performed to confirm mice infection. The subcutaneously DENV inoculated mice showed weight loss after infection, but they did not show any other clinical signs. The leukocytes and platelets decreased after subcutaneous inoculation. The cytokines TNF alpha and IFN gamma increased after infection in mice. The subcutaneous model provided scope for improved understanding of the dengue pathogenesis, as well as possible mechanism for protection to subsequent mouse infected by intracranial route in mice. This model could be used to study the vertebrate immune response and evaluation of drugs or vaccine against dengue virus.

关键词： Subcutaneous Murine Model Dengue Disease Immune Competent Mouse Cytokines Innate Immune Response

Comparison and Quantification of the Development of Phenolic Compounds during the Aging of Cachaca in Oak(Quercus sp)and Amburana(Amburana cearensis)Barrels

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American Journal of Plant Sciences 2014年第21期5卷 3140-3150页

作者： Wilder D.Santiago Maria das G.Cardoso Juliana de A.Santiago Marcos S.Gomes Leonardo M.A.rodrigues Rafaela M.Brandao Rodolfo R.Cardoso Gabriel B.d’Avila bruno L.da Silva Alex R.S.Caetano Department of Chemistry Federal University of LavrasLavrasBrazil Department of Food Science Federal University of LavrasLavrasBrazil

Aging is a stage of the cachaça production process in which several chemical reactions occur between compounds extracted from the wood and other compounds present on the beverage. In an attempt to establish a profile regarding the chemical characterization of aged cachaças, phenolic compounds have been studied because of the specific characteristics of each wood species and their known antioxidant properties. This work sought to assess and compare the development of 12 phenolic compounds in cachaças aged in oak (Quercus sp) and amburana (Amburana cearenses) barrels during a period of 12 months. There was a progressive increase in the concentration of phenolic compounds in the beverage for both of the types of wood. The principal compounds encountered in the cachaça aged in oak barrels were gallic acid, syringaldehyde and syringic acid, while vanillic acid, syringaldehyde, sinapic acid and gallic acid were isolated from that aged in amburana barrels.

关键词： Beverage Aging Phenolic Compounds Liquid Chromatography

Justicia insularis Improves the in vitro Survival and Development of Ovine Preantral Follicles Enclosed in Ovarian Tissue

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Journal of Pharmacy and Pharmacology 2017年第9期5卷 668-680页

作者： Gildas Tetaping Mbemya Denise Damasceno Guerreiro Nathalie Jiatsa Donfacl~ Luciana Mascena Silv~ Luis Alberto Vieira Geovania Francisca Canafistula de Sousa Benner Geraldo Alves Aryele Pinto Izaguirry Francielli Weber Santos Phelix bruno Telefo Otilia Deusdenia Loiola Pessoa Johan Smitz Jose Ricardo de Figueiredo Ana Paula Ribeiro rodrigues Faculty of Veterinary (FA VET) State University of Ceara Fortaleza 60714903 Brazil Laboratory of Reproduction Biotechnology (Biotech) State of University of Pampa Uruguaiana 97500970 Brazil Laboratory of Biochemistry of Medicinal Plants Food and Nutritional Sciences Faculty of Science University of DschangDschang 3451381 Cameroon Laboratory of Phytochemical Analysis of Medicinal Plants (LAFIPLAN) Federal University of Cear6 Fortaleza 60020181 Brazil Follicle Biology Laboratory Center for Reproductive Medicine Brussels 1090 Belgium

Objectives： Evaluating the addition effect of./. insularis extract and FSH on the survival, activation and ROS production after in vitro culture of ovine preantral follicles enclosed in ovarian tissue. Methods： In the first experiment, ovarian fragments were fixed （non-cultured control） or in vitro cultured in α-MEM＋（cultured control）, α-MEM＋ supplemented with FSH 50 ng/mL, or in α-MEM＋supplemented with J. insularis （JUS0.3; 1.25 or 5 mg/mL） for 1 or 7 days, at 39℃, 5% CO2. In the second experiment, fragments were fixed or cultured in α-MEM＋ supplemented with anethole 300 μg/mL ＋ FSH 50 ng/mL or in α-MEM＋ supplemented with anethole 300μg/mL ＋ 0.3 mg/mL JUS. Key findings： JUS0.3 was the only treatment that maintained the percentage of morphologically normal follicles similar to non-cultured control even after 7 days of culture. After 7 days of culture, a higher （p 〈 0.05） percentage of developing follicles was observed in JUS5 treatment compared with the other treatments except JUS 1.25. In the second experiment, FSH maintained the percentage of normal follicles and promoted activation of primordial follicles. A reduction （p 〈 0.05） of stromal cell density was observed in MEM＋＋ANE supplemented with JUS or FSH. Conclusions： J. insularis in a concentration-dependent manner maintained the levels of ROS and improved in vitro follicular survival and activation of ovine primordial follicles.

关键词： Medicinal plant antioxidant in vitro folliculogenesis preantral follicles.

The Phytochemical Profile and Photoprotective Potential of Bark and Leaves of Erythrina velutina Willd.—A Medicinal Species from the Caatinga Region of Brazil

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Journal of Biosciences and Medicines 2023年第12期11卷 325-342页

作者： bruno de Almeida Andrade Allan Jonathan Chernichiarro Corrêa Ana Klarissa Soares Gomes Patrícia Maria da Silva Neri Cruz Aline Michaele de Saint-George Albuquerque Cheron Valerium Thijan Nobre de Almeidae Castro Décio Henrique Araújo Salvadôr de Mello Cláudia Sampaio de Andrade lima Ricardo Yara Elba Lúcia Cavalcanti de Amorim Department of Pharmaceutical Sciences Federal University of Pernambuco Recife Brazil Pharmacy Course Mauricio de Nassau University Center Recife Brazil Department of Biophysics Federal University of Pernambuco Recife Brazil Departament of Biomedical Engineering Federal University of Pernambuco Recife Brazil

The use of medicinal plants as therapeutic and economic resources has been on the rise in recent years. In Brazil, however, increasing doubt is being cast on the quality of these products, owing to the prevalence of adulteration and fraud. Solar radiation can cause serious damage to human skin, as a result, mostly, of ultraviolet light, which is a cause of skin cancer. Photoprotective substances are capable of absorbing, reflecting, or refracting ultraviolet radiation and thus protecting skin from exposure to sunlight. The present study aimed to characterize samples and examine the phytochemical profile and photoprotective potential of bark and leaves of Erythrina velutina Willd. The samples underwent five extraction methods using 80% ethanol. The phenolic content was measured using spectrophotometry. Antioxidant activity was examined using the DPPH and the photoprotective properties of the plant extracts were assessed using the method developed by Mansur. There was a quantitative difference in some groups of metabolites, with higher levels of tannin in the bark and of flavonoids in the leaves. The latter showed greater DPPH free radical scavenging capacity than the bark, although higher levels of SPF were obtained from the bark, with no statistically significant differences between methods. The results indicate that Erythrina velutina Willd. has potential as a photoprotector.

关键词： Phytochemical Caatinga Phytochemistry Sun Protection Factor (SPF)

Scientific Evidence about the Assistance Provided to the Newborn with Meconium Elimination:Integrative Literature Review

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Open Journal of Obstetrics and Gynecology 2020年第4期10卷 504-515页

作者： Marília Cordeiro de Sousa Lívia Roberta rodrigues Conceicao Marcela de Andrade Silvestre Lays de Souza Silva Camila de Pina Soares Sudário Renata Calciolari Rossi Janaína Valadares Guimaraes Thaila Correa Castral Karina Machado Siqueira Pedro Teixeira Meireles bruno Belmonte Martineli Gomes George Kemil Abdalla Douglas Reis Abdalla Ana Karina Marques Salge Faculty of Nursing Federal University of GoiásGoianiaBrazil Western ParanáState University CascavelBrazil Medicine Course University of UberabaUberabaBrazil Biomedicine University of Sao PauloRibeirao PretoBrazil Health Sciences Faculty of Human TalentsUberabaBrazil

The Meconial Aspiration Syndrome (MAS) is a complication caused by the presence of meconium amniotic fluid with consequent aspiration, being responsible for important rates of perinatal morbidity and mortality. This study aims to analyze the bibliographical production on newborn care with meconium elimination in the delivery room. It was a literature integrative review electronically based. Most of the studies referred to tracheal intubation and exogenous surfactant ministration as main care methods. The Meconium Aspiration Syndrome is presented as main theme. It is understood that health care professionals must develop knowledge, skills and actions, showing conduct based on scientific, critical and reflexive knowledge with purposeful interventions directed to the patient’s needs.

关键词： Perinatal Care Meconium Aspiration Syndrome Newborn

Molecular and serology methods in the diagnosis of COVID-19: An overview

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World Journal of Methodology 2022年第3期12卷 83-91页

作者： Marcel Silva Luz Ronaldo Teixeira da Silva Júnior Gabriella Almeida Santos de Santana Gabriela Santosrodrigues Henrique de lima Crivellaro Mariana Santos Calmon Clara Faria Souza Mendes dos Santos LuisGuilherme de Oliveira Silva Qesya rodrigues Ferreira Guilherme Rabelo Mota Heloísa Heim Filipe AntônioFrança da Silva Breno Bittencourt de Brito Fabrício Freire de Melo Instituto Multidisciplinar em Saúde Universidade Federal da BahiaVitória da Conquista 45002175BahiaBrazil Instituto Multidisciplinar em Saúde Universidade Federal da BahiaVitória da Conquista 45029-094BahiaBrazil

Coronavirus disease-19(COVID-19)has become a pandemic,being a global health concern since December 2019 when the first cases were *** acute respiratory syndrome coronavirus 2,the COVID-19 causal agent,is aβ-coronavirus that has on its surface the spike protein,which helps in its virulence and pathogenicity towards the ***,effective and applicable diagnostic methods to this disease come as an important tool for the management of the *** use of the molecular technique PCR,which allows the detection of the viral RNA through nasopharyngeal swabs,is considered the gold standard test for the diagnosis of ***,serological methods,such as enzyme-linked immunosorbent assays and rapid tests,are able to detect severe acute respiratory syndrome coronavirus 2-specific immunoglobulin A,immunoglobulin M,and immunoglobulin G in positive patients,being important alternative techniques for the diagnostic establishment and epidemiological *** the other hand,reverse transcription loop-mediated isothermal amplification also proved to be a useful diagnostic method for the infection,mainly because it does not require a sophisticated laboratory apparatus and has similar specificity and sensitivity to ***,imaging exams provide findings of typical pneumonia,such as the ground-glass opacity radiological pattern on chest computed tomography scanning,which along with laboratory tests assist in the diagnosis of COVID-19.

关键词： COVID-19 Pandemic Diagnosis Polymerase chain reaction Molecular biology Serology

Gravity Fault Subsidence and Beach Ridges Progradation in Quinta-Cassino (RS) Coastal Plain, Brazil

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Open Journal of Geology 2024年第2期14卷 177-195页

作者： bruno Silva da Fontoura Adelir José Strieder Iran Carlos Stalliviere Corrêa Paulo Rogério Mendes Alexandre Felipe Bruch Angélica Cirolini Programa de Pós-Graduaç &#227o em Geoci&#234ncias (PPGeo) Instituto de Geoci&#234ncias UFRGS Porto Alegre Brazil Engenharia Geológica Centro de Engenharias UFPel Pra&#231a Domingos Rodrigues 2 Pelotas Brasil HIDROSERV-Serviç os Geológicos e Geofísicos Ltda. Rua Senegal 152 Porto Alegre Brazil Colégio Politécnico UFSM Santa Maria Brasil

Ground penetrating radar (GPR) surveys have being applied to investigate very near-surface stratification of sedimentary units in coastal plains and to define their depositional conditions. This paper presents, however, low-frequency GPR survey to investigate fault-related depositional systems at greater depths. The Quinta-Cassino area in the Rio Grande do Sul Coastal Plain (RGSCP, Brazil) shows a wide strandplain that is made off by very long, continuous, and linear geomorphic features (beach ridges). This strandplain extends for ~70 km southward. The beach ridges show low-angle truncations against the Quinta escarpment, and also truncations in the strandplain. The traditional approach points that RGSCP was developed by juxtaposition of four lagoons/barrier systems as consequence of sea level changes;previous model assumes that no deformational episode occurred in RGSCP. The geophysical and geological surveys carried out in this area showed the existence of listric fault controlling the beach ridges in the escarpments and hanging-wall blocks. The radargrams could distinguish Pleistocene basement unit anticlockwise rotation, thickening of beach ridges radarfacies close to listric normal faults, and horst structures. These deformational features indicate that the extensional zone of a large-scale gravity-driven structure controlled the mechanical subsidence, the Holocene sedimentation and its stratigraphic and geomorphic features in the Quinta-Cassino area to build up an asymmetric delta. The results point to a new approach in dealing with RGSCP Holocene evolution.

关键词： Gravity Tectonics Normal Faults Ground Penetrating Radar Survey Beach-Ridges Progradation

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医院小丑在儿科症状管理中的效果

英国医学杂志中文版 2021年第10期24卷 606-608页

作者： Lufs Carlos Lopes-Junior Emiliana Bomfim Karin Olson Eliane Tatsch Neves Denise Sayuri Calheiros Silveira Michelle Darezzo rodrigues Nunes Lucila Castanheira Nascimento Gabriela Pereira-da-Silva Regina Aparccida Garcia lima 蔡思雨(译) 彭晓霞(校) Federal University of Espirito Santo Avenida Marechai CampusES.Brazil University of Saskatchewan College of Medicine.SaskatoonSKCanada University of Alberta EdmontonABCanada Federal University of Santa Maria Santa Maria.RSBrazil Federal Institute of Espirito Santo LinharesESBrazil Rio de Janeiro State University Rio de Janeiro.RJ.Brazil University of Sao Paulo at Ribeirao Prelo College of Nursing SPBrazil 国家儿童医学中心首都医科大学附属北京儿童医院临床流行病学与循证医学中心

目的基于随机对照试验和非随机对照试验产生的证据,在因急慢性疾病住院的儿童和青少年中,评价医院小丑对一系列症状群的干预效果。研究设计基于随机和非随机对照试验的系统综述。数据来源美国国立医学图书馆(MEDLINE).美国科学情报研究... 详细信息

目的基于随机对照试验和非随机对照试验产生的证据,在因急慢性疾病住院的儿童和青少年中,评价医院小丑对一系列症状群的干预效果。研究设计基于随机和非随机对照试验的系统综述。数据来源美国国立医学图书馆(MEDLINE).美国科学情报研究所数据库(ISI of Knowledge).考科蓝对照试验注册中心(Cochrane Cental Registerof Controled Trials)、Science Diret全文数据库、Sopus数据库、美国心理协会数据库(American Psychalogical Association PeyeINFO)、护理和健康文献累积指数数据库(Curmulative Index to Nursing and Alied Heauth Literature),以及拉丁美洲和加勒比健康科学文献数据库(Lain Ameriean and Caribbean Health Sciences Literature)。研究筛选经間行评议后筛选随机和非随机对照试脸,纳人标准如下:因急性或慢性疾病人院的儿童和青少年,比较医院小丑与标准护理的研究,此外研究将医院小丑对住院儿童和青少年症状管理的影响作为主要结局指标。数据提取与整合由两名研究者独立完成研究筛选,数据摘录,以及偏倚风险评价。方法学评价也由两名研究者独立进行,评价工具有:Jadnd量表、随机对照试验的偏倚风险评估工具(RoB 2)和非随机对照试验的偏倚风险评估工具(ROBINS-I)。结果24项研究(n=1612)符合纳入标准,对其进行数据摘录和分析。大多数研究是随机对照试验(n=13)。最常分析的症状是焦虑(n=13),其次是疼痛(n=9)、心理和情绪反应以及幸福感(n=4)、压力(n=4)、癌症相关疲劳(n-3)和哭泣(n=2)。5项研究使用生物标志物(主要为皮质醇)来评估医院小丑对压力或疲劳的影响。方法学评价显示,大多数随机对照试验(n=11;85%)存在潜在偏倚风险,2项试验具有高度偏倚风险。ROBINS-I评价结果显示,大多数非随机对照试验(n=6;55%)存在中度偏倚风险。研究表明,无论父母是否在场,医院小丑出现时,儿童和青少年在一系列医疗程序中的焦虑显著降低,心理调节也得到改善(P<0.05)。3项针对慢性病患者的研究显示,医院小丑的干预有明显获益,显著减少了患儿的压力、疲劳、疼痛和痛营(PC0.05)。结论这些研究表明,在医疗程序术前诱导麻醉,以及慢性病常规护理中进行医院小丑干预,可能是症状管理的有效策略。此外,与接受标准护理的患儿相比,医院小丑干预可能有助于改善患有急慢性疾病的儿童和青少年的心理健康状态。

关键词：急慢性疾病方法学评价慢性病患者心理健康状态诱导麻醉症状群情报研究所文献数据库

Evaluation of antibacterial and toxicological activities of essential oil of Ocimum gratissimum *** its major constituent eugenol

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Food Bioscience 2022年第6期50卷 2152-2159页

作者： Julio Cesar Silva Raimundo Luiz Silva Pereira Thiago Sampaio de Freitas Janaína Esmeraldo Rocha Nair Silva Macedo Carla de Fatima Alves Nonato Marina Leite Linhares Daniely Sampaio Arruda Tavares Francisco Assis Bezerra da Cunha Henrique Douglas Melo Coutinho Sidney Gonçalo de lima Francisco Nascimento Pereira-Junior Francisco Paulo Araújo Maia Ivo Cavalcante Pita Neto Fabiola Fernandes Galvão rodrigues George Joaquim Garcia Santos Department of Biological Chemistry Regional University of CaririR.Cel.Antonio Luis 116163105000CratoCEBrazil University Center Doutor Leão Sampaio Av.Leão Sampaio 40063040000Juazeiro do NorteCEBrazil Research and Development Center for Medicines Federal University of CearaR.Coronel Nunes de Melo 100060430275FortalezaCEBrazil Chemistry Department Federal University of PiauiCampus Universitário Ministro Petrônio Portella64049550TeresinaPIBrazil Center for Agricultural and Biodiversity Sciences - CCAB Federal University of CaririCratoCearáBrazil CECAPE - College of Dentistry Juazeiro do NorteCE63024-015Brazil

The present study aimed to comparatively analyze the antibacterial activity,combined with antibiotics,and toxicological activity of the essential oil of *** *** its major compound,*** phytochemical analysis of the essential oil was performed by gas chromatography coupled with mass *** minimum inhibitory concentration and combined antibiotic activity were performed by *** melanogaster was the model organism chosen to evaluate the toxicological response of *** chromatographic assay showed eugenol as the major compound of the essential oil of *** *** microbiological assays showed that in terms of antibacterial activity,both products showed similar activity,quite significant in Gram-negatives,such as those of strains of Pseudomonas *** results were verified in the modulatory activity,allowing the verification that the bioactivity performed by the essential oil of *** *** mainly influenced by its major *** the EC50 of the compounds varied subtly,the essential oil was less toxic compared to *** information obtained in the present study demonstrates that the essential oil of *** *** a promising antibacterial agent and better tolerated than its majority compound alone,in the model organism ***.

关键词： Antibacterial agents Drosophila melanogaster Eugenol Ocimum gratisismumum L.essential oils