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Polyethylene glycol restores axonal conduction after corpus callosum transection

Neural Regeneration Research 2017年第5期12卷 757-760页

作者： Ravinder Bamba d.Colton Riley Richard B.Boyer Alonda C.Pollins R.bruce Shack Wesley P.Thayer department of Plastic Surgery Vanderbilt University Medical Center Nashville TN USA department of Surgery Georgetown University Washington DC USA Georgetown University School of Medicine Washington DC USA

Polyethylene glycol（PEG） has been shown to restore axonal continuity after peripheral nerve transection in animal models. We hypothesized that PEG can also restore axonal continuity in the central nervous system. In this current experiment, coronal sectioning of the brains of Sprague-dawley rats was performed after animal sacrifice. 3Brain high-resolution microelectrode arrays（MEA） were used to measure mean firing rate（MFR） and peak amplitude across the corpus callosum of the ex-vivo brain slices. The corpus callosum was subsequently transected and repeated measurements were performed. The cut ends of the corpus callosum were still apposite at this time. A PEG solution was applied to the injury site and repeated measurements were performed. MEA measurements showed that PEG was capable of restoring electrophysiology signaling after transection of central nerves. Before injury, the average MFRs at the ipsilateral, midline, and contralateral corpus callosum were 0.76, 0.66, and 0.65 spikes/second, respectively, and the average peak amplitudes were 69.79, 58.68, and 49.60 μV, respectively. After injury, the average MFRs were 0.71, 0.14, and 0.25 spikes/second, respectively and peak amplitudes were 52.11, 8.98, and 16.09 μV, respectively. After application of PEG, there were spikes in MFR and peak amplitude at the injury site and contralaterally. The average MFRs were 0.75, 0.55, and 0.47 spikes/second at the ipsilateral, midline, and contralateral corpus callosum, respectively and peak amplitudes were 59.44, 45.33, 40.02 μV, respectively. There were statistically differences in the average MFRs and peak amplitudes between the midline and non-midline corpus callosum groups（P 〈 0.01, P 〈 0.05）. These findings suggest that PEG restores axonal conduction between severed central nerves, potentially representing axonal fusion.

关键词： nerve regeneration polyethylene glycol nerve repair axonal fusion central nerve injury axonal conduction corpus callosum neural regeneration

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YPEd: An Integrated Bioinformatics Suite and database for Mass Spectrometry-based Proteomics Research

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Genomics, Proteomics & Bioinformatics 2015年第1期13卷 25-35页

作者： Christopher M.Colangelo Mark Shifman Kei-Hoi Cheung Kathryn L.Stone Nicholas J.Carriero Erol E.Gulcicek TuKiet T.Lam Terence Wu Robert d.Bjornson Can bruce Angus C.Nairn Jesse Rinehart Perry L.Miller Kenneth R.Williams W.M.Keck Foundation Biotechnology Resource Laboratory School of Medicine Yale University department of Molecular Biophysics & Biochemistry Yale University Yale Center for Medical Informatics School of Medicine Yale University department of Anesthesiology School of Medicine Yale University department of Emergency Medicine School of Medicine Yale University VA Connecticut Healthcare System W.M. Keck Foundation Biotechnology Resource Laboratory School of Medicine Yale University department of Computer Science Yale University Yale Center for Genome Analysis West Campus Yale University Yale West Campus Analytical Core West Campus Yale University Yale Bioinformatics Resource School of Medicine Yale University department of Psychiatry School of Medicine Yale University department of Cellular & Molecular Physiology School of Medicine Yale University Systems Biology Institute Yale University

We report a significantly-enhanced bioinformatics suite and database for proteomics research called Yale Protein Expression database（YPEd） that is used by investigators at more than 300 institutions worldwide. YPEd meets the data management, archival, and analysis needs of a high-throughput mass spectrometry-based proteomics research ranging from a singlelaboratory, group of laboratories within and beyond an institution, to the entire proteomics community. The current version is a significant improvement over the first version in that it contains new modules for liquid chromatography–tandem mass spectrometry（LC–MS/MS） database search results, label and label-free quantitative proteomic analysis, and several scoring outputs for phosphopeptide site localization. In addition, we have added both peptide and protein comparative analysis tools to enable pairwise analysis of distinct peptides/proteins in each sample and of overlapping peptides/proteins between all samples in multiple datasets. We have also implemented a targeted proteomics module for automated multiple reaction monitoring（MRM）/selective reaction monitoring（SRM） assay development. We have linked YPEd＇s database search results and both label-based and label-free fold-change analysis to the Skyline Panorama repository for online spectra visualization. In addition, we have built enhanced functionality to curate peptide identifications into an MS/MS peptide spectral library for all of our protein database search identification results.

关键词： Proteomics database Bioinformatics Mass spectrometry Repository Spectral library

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油菜黑胫病原真菌的分离与鉴定

油菜黑胫病原真菌的分离与鉴定

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中国植物病理学会2008年学术年会

作者：刘泽李强生 Avice M.Hall bruce d.L.Fitt 安徽省农业科学院作物研究所 University of Hertfordshire HatfieldHertfordshire AL10 9ABUK Rothamsted Research HarpendenHertfordshire AL5 2JQUK

在2005～2007年,从我国一些油菜产区获得油菜黑胫病害感染植株,病原物分离鉴定的结果显示,其致病真菌为侵染性较弱的 Leptosphaeria biglobosa,具有强侵染性的菌种 *** 尚未从上述产区获得。在菌落形态上(包括在营养基质上的生长速度和... 详细信息

在2005～2007年,从我国一些油菜产区获得油菜黑胫病害感染植株,病原物分离鉴定的结果显示,其致病真菌为侵染性较弱的 Leptosphaeria biglobosa,具有强侵染性的菌种 *** 尚未从上述产区获得。在菌落形态上(包括在营养基质上的生长速度和色素合成等),中国的 *** 菌株与欧洲的 *** 菌株具有很大相似性。

关键词：油菜油菜黑胫病油菜黑胫病菌

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三种3d音频增强器给多媒体PC带来环绕立体声效果

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个人电脑 1996年第8期2卷 32-33页

作者： bruce Brown 黄国胜

如果你希望加强计算机中演示图片的效果,或者希望在聆听音频Cd或玩游戏时获得更加美妙的声音,那么你可以加入3-d声音,因为3-d音效的加入将产生戏剧性的改进效果。3-d声音电路有时被集成到主板上,有时也被设计在声卡中或计算机扬声器系... 详细信息

如果你希望加强计算机中演示图片的效果,或者希望在聆听音频Cd或玩游戏时获得更加美妙的声音,那么你可以加入3-d声音,因为3-d音效的加入将产生戏剧性的改进效果。3-d声音电路有时被集成到主板上,有时也被设计在声卡中或计算机扬声器系统中。

关键词：环绕立体声多媒体PC 增强器立体声信号音频卡输出插孔计算机扬声器系统 Imager 音频设备

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简化A／d转换的新技术

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电子与仪表 1990年第6期 29-31,40页

作者： bruce.,I 木禾

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Monitoring real time polymorphic transformation of sulfanilamide by diffuse reflectance visible spectroscopy

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Journal of Pharmaceutical Analysis 2016年第3期6卷 179-183页

作者： Tracy O.Ehiwe bruce d.Alexander John C.Mitchell Martin J.Snowden Laura J.Waters Medway Centre for Formulation Science Faculty of Engineering and Science University of Greenwich at Medway School of Applied Sciences University of Huddersfield

This study investigated the development of a novel approach to surface characterization of drug poly- morphism and the extension of the capabilities of this method to perform ＇real time＇ in situ measure- ments. This was achieved using diffuse reflectance visible （dRV） spectroscopy and dye deposition, using the pH sensitive dye, thymol blue （TB）. Two polymorphs, SFN-β and SFN-γ, of the drug substance sulfanilamide （SFN） were examined. The interaction of adsorbed dye with polymorphs showed different behavior, and thus reported different dRV spectra. Consideration of the acid/base properties of the morphological forms of the drug molecule provided a rationalization of the mechanism of differential coloration by indicator dyes. The kinetics of the polymorphic transformation of SFN polymorphs was monitored using treatment with TB dye and dRV spectroscopy. The thermally-induced transformation fitted a first-order solid-state kinetic model （R2=0.992）, giving a rate constant of 2.43 × 10^- 2 s 1.

关键词： Polymorphic transformation Sulfanilamide diffuse reflectance visible spectroscopy Powder X-ray diffraction differential scanning calorimetry

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抗艾战役关键一步

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环球科学 2012年第8期 38-45页

作者：布鲁斯·d·沃克(bruce d.Walker) 王宇歌(翻译) 张林琦(审校) 不详清华大学艾滋病综合研究中心的研究生清华大学长江特聘教授

绝大部分HIV感染者，都会慢慢发展成艾滋病患者。但极少一部分HIV感染者却有非凡的免疫控制力，他们不经任何治疗，即能把体内的病毒长期控制在很低的水平，不会发展成艾滋病。研究这些非凡控制者的免疫机制，不仅能为治愈艾滋病带来启... 详细信息

绝大部分HIV感染者，都会慢慢发展成艾滋病患者。但极少一部分HIV感染者却有非凡的免疫控制力，他们不经任何治疗，即能把体内的病毒长期控制在很低的水平，不会发展成艾滋病。研究这些非凡控制者的免疫机制，不仅能为治愈艾滋病带来启示，也为研制出艾滋病疫苗带来希望。

关键词： HIV感染者艾滋病患者战役艾滋病疫苗免疫

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Scanless multitarget-matching multiphoton excitation fluorescence microscopy

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Journal of Innovative Optical Health Sciences 2018年第2期11卷 1-7页

作者： Junpeng Qiu Lei Wang bruce Zhi Gao Junle Qu Yonghong Shao College of Optoelectronics Engineering Key Laboratory of Optoelectronic devices and Systems of Ministry of Education and Guangdong Province Shenzhen University Shenzhen 518060P.R.China department of Bioengineering and COMSET Clemson University ClemsonSC 29634USA

Using the combination of a refective blazed grating and a reflective phase-only difractive spatiallight modulator(SLM),scanless multitarget-matching multiphoton excitation fuorescence ***(SMTM-MP M)was *** SLM shaped an incoming mode-locked,near-infraredTi:sapphire laser beam into an excitation pattern with addressable shapes and sizes that matchedthe samples of interest in the field of *** and spatial focusing were simultaneouslyrealized by combining an objective lens and a blazed *** fluorescence signal fromilluminated areas was recorded by a two-dimensional sCMOS *** with a conventional temporal focusing multiphoton microscope,our microscope achieved effective use of thelaser power and decreased photodamage with higher axial resolution.

关键词： Multitarget-matching multiphoton microscopy SLM temporal focusing

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船舶建造

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作者： (英)大卫·约翰·艾尔斯(d.J. Eyres)，(英)乔治·约翰·布鲁斯(G.J. bruce)

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Temporal evolution of scour at bridge abutments in compound channels

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International Journal of Sediment Research 2022年第5期37卷 662-674页

作者： Yifan Yang bruce W.Melville Xiaozhou Xiong Lu Wang School of Engineering The University of Waikato Risland New Zealand department of Civil and Environmental Engineering The University of Auckland State Key Laboratory of Hydraulics and Mountain River Engineering College of Water Resource and Hydropower Sichuan University

Bridge abutment-induced sediment scour in compound river channels is time-sensitive during floods, and, thus, estimating the scour rate has significance in design. In the current study, large-scale laboratory experiments were done to examine the influence of multiple factors on scour evolution,including channel geometry, bed mobility(clear-water or live-bed), and lateral(embankment intrusion) and vertical(deck submergence) flow contractions. A new method is applied for scour measurement and determination of characteristic scour trends. Results show that the nondimensional time scale is related to flow intensity and may peak before reaching the clear-water scour threshold; the corresponding equations also are derived. It is found that the use of scour countermeasures has a significant impact on the scour rate, and the existing equations for unprotected abutments have limitations. Under clear-water conditions, the scour rate is closely related to the overall flow contraction, which can be better reflected by the ratio of the approaching unit discharge to that at the bridge section. Specifically, the effect of vertical flow contraction on scour rate is more dominant than other factors. Two scour rate curves are derived to suit different structural and flow contraction types for clear-water scour. In contrast, the live-bed scour rate data are generally consistent and fall in a narrow band, showing less dependence on other factors. Finally, a new design approach is established to estimate the time scale and scour rate using the new equations proposed in this paper.

关键词： Abutment scour Temporal evolution Compound channel Time scale Scour rate

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