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Animal Nutrition 2021年第1期7卷 64-71页

作者： Susanto Prawirodigdo Neil j.Gannon brian j.leury Frank R.Dunshea Faculty of Veterinary and Agricultural Sciences The University of MelbourneParkvilleVictoria3010Australia Balai Pengkajian Teknologi Pertanian Central java SemarangCentral Java50552Indonesia Biomin Singapore PO Box 2419ToowoombaQLD4350Australia Faculty of Biological Sciences The University of LeedsLeedsLS29JTUnited Kingdom

The aim of this study was to determine the apparent total tract digestibility(ATTD)of nutrients in cottonseed meal(CSM)and soybean meal(SBM)in simple carbohydrate and more complex wheat-based diets using 2 indigestible markers and total faecal ***-five Large White×Landrace boars(57.8 kg)were randomly allocated to either a pure wheat diet,40%CSM or SBM in either a sugar-starch-(1∶1)or wheat-based diet for 18 ***-insoluble ash(AIA)and chromic oxide(Cr2O3)were included in all diets as indigestible *** were offered(1,800 g/d per pig)in 3 meals/d from d 1 to 11 and 8 meals/d from d 12 to *** d 9,the pigs were moved to individual metabolism cages to allow total faecal *** d 18,the pigs were fed hourly for 8 *** the 8th meal,pigs were anaesthetized and digesta sampled from the terminal ileum and rectum before lethal *** were no differences between ATTD of nitrogen(N)determined using AIA as a marker and measured by total faecal *** the other hand,the ATrD of N of diets containing CSM in sugar-starch-or wheat-based diets and the pure wheat diet determined using Cr2O3 as a marker was less(-3.11%,-4.46%and-6.59%;P

关键词： Organic matter Pig Wheat Cottonseed meal Soybean meal Digestibility Nitrogen

Basal diet and indigestible marker influence apparent digestibilities of nitrogen and amino acids of cottonseed meal and soybean meal in pigs

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Animal Nutrition 2019年第3期5卷 234-240页

作者： Susanto Prawirodigdo Neil j.Gannon brian j.leury Frank R.Dunshea Faculty of Veterinary and Agricultural Sciences The University of MelbourneParkvilleVictoria3010Australia BPTP Central java SemarangCentral Java50552Indonesia Biomin Singapore PO Box 2419ToowoombaQLD4350Australia

This study was to determine the apparent ileal nitrogen(N)and amino acid digestibilities of cottonseed meal(CSM)and soybean meal(SBM)in simple carbohydrate-based and more complex wheat-based *** five Large White × Landrace boars(40.9 kg)were randomly allocated to one of 5 dietary treatments:a wheat diet,40% CSM in either a sugar:starch(1:1)or wheat-based diet,or 40% SBM in either a sugar:starch or wheat-based diet for 14 *** diets contained vitamins,minerals,and contained acid-insoluble ash(AIA)and Cr2 O3 as indigestible *** were offered(1,800 g/pig per d)in3 meals/d on d 1-11 and 8 meals/d on d *** d 14,the pigs were fed hourly for 8 *** the 8 th meal,pigs were anaesthetised with isoflurothane,and ileal and faecal digesta sampled from the terminal ileum and rectum before lethal injection of *** ileal digestibility(AID)of N was greater(12.1%)when CSM was fed in the wheat-based diet as compared to the simple carbohydrate-based diet,whereas apparent ileal N digestibility of SBM was slightly lower(-4.5%)in the wheat-based diet as compared to the sugar:starch-based *** ileal amino acid digestibility generally respondeds similarly to ***,while there was a wide difference in apparent N and amino acid digestibilities of SBM and CSM when they were fed in the sugar:starch-based diets,these differences were less apparent when they were fed the wheat-based *** was an apparent net release of N into the hindgut of pigs fed CSM in both base ***,there was quite substantial apparent digestion of N in the hindgut of pigs fed SBM in both base *** use of Cr2 O3 as an indigestible marker resulted in lower apparent ileal and faecal digestibilities than using AIA,particularly for diets containing *** data demonstrate that the basal diet and choice of indigestible marker can substantially influence the estimate of apparent N digestibility,this response can differ for different protein meals.

关键词： Organic matter Pig Wheat Cottonseed meal Soybean meal Digestibility

Dietary nano chromium picolinate can ameliorate some of the impacts of heat stress in cross-bred sheep

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Animal Nutrition 2021年第1期7卷 198-205页

作者： Alex T.Hung brian j.leury Matthew A.Sabin Fahri Fahri Kristy DiGiacomo Tu-Fa Lien Frank R.Dunshea Faculty of Veterinary and Agricultural Sciences The University of MelbourneParkvilleVIC3010Australia Murdoch Children's Research Institute The University of MelbourneParkvilleVIC3010Australia Department of Animal Science National Chiayi UniversityChina Faculty of Biological Sciences The University of LeedsLeeds LS29JTUnited Kingdom

Two studies were conducted to evaluate the effect of nano chromium picolinate(nCrPic)during heat stress(HS)in *** the initial study,36 Merino×Poll cross-bred sheep were individually penned and allocated to 3 dietary treatments(0,400 and 800μg/kg nCrPic)for 8 *** composition was determined at the beginning and end of the experiment using dual energy X-ray *** sheep remained in their dietary groups but were then placed in metabolic cages and randomly allocated within the dietary group to differing ambient temperature regimes,i.e.,thermo-neutral(TN)(n=18)and HS(n=18),for 3 *** nCrPic had no effect on growth performance and body composition during the initial study conducted under TN *** stress decreased average daily feed intake(ADFI)(P=0.002)whereas sheep under HS had reduced average daily gain(ADG)and indeed lost weight(P<0.001).Dietary nCrPic increased both ADFI(P=0.041)and ADG(P=0.049)under both TH and HS conditions such that the performance of sheep receiving supplemental nCrPic and exposed to HS was similar to that of control sheep maintained under TN *** stress increased rectal temperature(P<0.001)and respiration rate(P<0.001),particularly during the hottest parts of the day as indicated by interactions(P<0.001)between time of day and thermal *** temperature was lower in sheep fed nCrPic(P=0.050),particularly under peak HS conditions during the afternoon as indicated by the interactions between dietary nCrPic and time of day(P<0.001)and dietary nCrPic,thermal treat-ment and time of day(P=0.010).Similarly,respiration rate was lower in sheep fed nCrPic under peak HS conditions during the afternoon as indicated by the interactions between dietary nCrPic and thermal treatment(P<0.001)and dietary nCrPic and time of day(P=0.030).In conclusion,dietary nCrPic can partially ameliorate the negative effects of HS as indicated by the maintenance of ADFI and decreased physiological responses,such as elevatio

关键词： Chromium picolinate Sheep Heat stress Nanotechnology Physiology

Nano-chromium picolinate and heat stress enhance insulin sensitivity in cross-bred sheep

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Animal Nutrition 2023年第2期13卷 173-184页

作者： Alex T.Hung brian j.leury Matthew A.Sabin Fahri Fahri Kristy DiGiacomo Tu-Fa Lien Frank R.Dunshea Faculty of Veterinary and Agricultural Sciences The University of MelbourneParkvilleVIC 3010Australia Murdoch Children's Research Institute The University of MelbourneParkvilleVIC 3010Australia Department of Animal Science National Chiayi UniversityChiayiChina Faculty of Biological Sciences The University of LeedsLeeds LS29JTUnited Kingdom

This study evaluated the effects of heat stress(HS)and dietary nano chromium picolinate(nCrPic)on metabolic responses of sheep to an intravenous glucose tolerance test(IVGTT),an intravenous insulin tolerance test(ITT)and an intramuscular adrenocorticotropin hormone(ACTH)challenge in ***-six sheep housed in metabolic cages were randomly allocated within 3 dietary groups(0,400 and 800 mg/kg supplemental nCrPic)to either thermoneutral(22℃)or cyclic HS(22 to 40℃)conditions for 3 *** plasma glucose tended to be increased during HS(P=0.052)and decreased by dietary nCrPic(P=0.013)while plasma non-esterified fatty acid concentrations were decreased(P=0.010)by ***-etary nCrPic reduced the plasma glucose area under the curve(P=0.012)while there were no significant effects of HS on plasma glucose area under the curve in response to the *** plasma insulin response over the first 60 min after the IVGTT was decreased by HS(P=0.013)and dietary nCrPic(P=0.022)with the effects being *** response to the ITT plasma glucose reached a nadir sooner(P=0.005)in sheep exposed to HS,although there was no effect on the depth of the *** nCrPic decreased(P=0.007)the plasma glucose nadir after *** the duration of the ITT plasma insulin concentrations were lower in sheep exposed to HS(P=0.013)whereas there was no significant effect of supplemental *** was no effect of either HS or nCrPic on cortisol response to *** nCrPic supple-mentation decreased(P=0.013)mitogen-activated protein kinase-8(jNK)and increased(P=0.050)carnitine palmitoyltransferase 1B(CPT1B)mRNA expression in skeletal *** of this experiment demonstrated that animals under HS and supplemented with nCrPic had greater insulin sensitivity.

关键词： Chromium picolinate Sheep Heat stress Insulin sensitivity

Gestational heat stress alters skeletal muscle gene expression profiles and vascularity in fetal pigs in a sexually dimorphic manner

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journal of Animal Science and Biotechnology 2022年第6期13卷 1785-1798页

作者： Weicheng Zhao Mark P.Green Christina D.Marth Fan Liu Hieu H.Le Gordon S.Lynch Alan W.Bell brian j.leury Frank R.Dunshea jeremy j.Cottrell School of Agriculture and Food Faculty of Veterinary and Agricultural SciencesThe University of MelbourneParkvilleVIC 3010Australia School of BioSciences Faculty of ScienceThe University of MelbourneParkvilleVIC 3010Australia Melbourne Veterinary School Faculty of Veterinary and Agricultural SciencesThe University of MelbourneWerribeeVIC 3030Australia Rivalea Australia Pty Ltd CorowaNSW 2646Australia Centre for Muscle Research Department of Anatomy and PhysiologyThe University of MelbourneParkville 3010Australia Department of Animal Science Cornell UniversityIthacaNY 14853-4801USA Faculty of Biological Sciences The University of LeedsLeeds LS29JTUK

Background: There is evidence that sow heat stress(HS) during gestation affects fetal development with implications for impaired muscle growth. We have previously demonstrated that maternal HS during early to midgestation compromised muscle fibre hyperplasia in developing fetal pigs. Thus, we hypothesised these phenotypic changes are associated with a change in expression of genes regulating fetal skeletal muscle development and metabolism. To test this, at d 60 of gestation, RNA sequencing and immunohistochemistry were performed on fetal longissimus dorsi(LD) muscle biopsies collected from pregnant gilts that had experienced either thermoneutral control(CON, 20 ℃, n = 7 gilts, 18 LD samples) or controlled HS(cyclic 28 to 33 ℃, n = 8 gilts, 23 LD samples)conditions for 3 ***: A total of 282 genes were differentially expressed between the HS and CON groups in female LD muscles(false discovery rate(FDR) ≤ 0.05), whereas no differentially expressed genes were detected in male LD muscles between the two groups(FDR > 0.05). Gestational HS increased the expression of genes associated with transcription corepressor activity, adipogenesis cascades, negative regulation of angiogenesis and pro-inflammatory signalling in female LD muscles. Immunohistochemical analyses revealed a decreased muscle vascularity density in fetuses from HS group for both sexes compared to those from the CON group(P = 0.004).Conclusions: These results reveal gilt HS during early to mid-gestation altered gene expression profiles in fetal LD muscles in a sexually dimorphic manner. The molecular responses, including transcription and angiogenesis repressions and enhanced adipogenesis cascades, were exclusively observed in females. However, the associated reductions in muscle vascularity were observed independently of sexes. Collectively this may indicate female fetal pigs are more adaptive to gestational HS in terms of gene expression changes, and/or there may be sexually dimorphic differences

关键词： Adipogenesis Angiogenesis Fetal pig Gestation Heat stress Sexual dimorphism Skeletal muscle Sows

Emerging role of A-kinase anchoring protein 5 signaling in reward circuit function

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Neural Regeneration Research 2025年第10期20卷 2913-2914页

作者： William j.Flerlage Mark L.Dell’Acqua brian M.Cox Fereshteh S.Nugent Uniformed Services University of the Health Sciences Department of Pharmacology and Molecular TherapeuticsBethesdaMDUSA Department of Pharmacology University of Colorado School of MedicineAnschutz Medical CampusAuroraCOUSA

There is a strong evidence supporting the hypothesis of synaptic dysfunction as a major contributor to neural circuit and network disruption underlying emotional and mood dysregulation in psychiatric disorders(Simmons et al.,2024).Diverse sets of distinct molecular signaling pathways converge on the synapse to regulate synaptogenesis,synaptic function,and synaptic plasticity in brain regions and circuits through complex interactions organized by numerous multivalent protein scaffolds,including the family of proteins known as A-kinase anchoring proteins(AKAPs).

关键词： function supporting

Dietary lecithin improves feed efficiency without impacting meat quality in immunocastrated male pigs and gilts fed a summer ration containing added fat

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Animal Nutrition 2018年第2期4卷 203-209页

作者： Henny Akit Cherie Collins Fahri Fahri Alex Hung Darryl D'Souza brian leury Frank Dunshea Faculty of Veterinary and Agricultural Sciences The University of Melbourne Department of Animal Science Faculty of Agriculture Universiti Putra Malaysia Rivalea Australia Pty Ltd. Australian Pork Ltd.

The aim of this study was to investigate the effects of sex and dietary lecithin on growth performance,meat quality, muscle collagen content and gene expression of key genes involved in collagen synthesis in finisher pigs.A total of 256 pigs(Large White × Landrace) were allotted to a 2 × 2 factorial arrangement involving sex(gilt or immunocastrated [IC] male) and dietary treatment(0 or 5 g/kg of dietary lecithin).All diets were formulated to contain 4.6% tallow with relatively high total fat of 6.3%.After 5 weeks of dietary treatment, pigs were slaughtered and Longissimus dorsi muscle was obtained for evaluation of meat quality and collagen *** abdominis muscle was analysed for gene expression of key genes involved in collagen synthesis namely, type Ⅰ(a1) procollagen(COL1A1), type Ⅲ(a1) procollagen(COL3A1), a-subunit of prolyl 4-hydroxylase(P4H), lysyl oxidase and metalloproteinase-1(MMP-1).The results showed that lecithin improved feed efficiency of all pigs(P 0.05).Lecithin also had no effect on meat compression, shear force, collagen content and gene expression(P > 0.05).Immunocastrated male had higher growth rate and increased COL1 A1 expression than ***, sex had no effect on fat depth at the P2 site(65 mm from the midline over the last rib), collagen content and expression of other genes(P > 0.05).In conclusion, lecithin improved feed efficiency in finishing pigs without impacting pork ***, inclusion of lecithin in diets containing high amount of tallow during the summer period could be beneficial.

关键词： Fat Feed efficiency Lecithin Meat quality Pigs

Effect of Air Plasma Processing on the Adsorption Behaviour of Bovine Serum Albumin on Spin-Coated PMMA Surfaces

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journal of Bionic Engineering 2008年第3期5卷 204-214页

作者： Chaozong Liu brian j. Meenan Advanced Materials and Biomaterials Research Centre School of Engineering The Robert Gordon University Aberdeen ABIO 1FR UK Northern ireland Bioengineering Centre University of Ulster Newtownabbey BT37 OQB Northern Ireland UK

This paper reports the adsorption of Bovine Serum Albumin （BSA） onto Dielectric Barrier Discharge （DBD） processed Poly（methyl methacrylate）（PMMA） surfaces by a Quartz Crystal Microbalance with Dissipation monitoring （QCM-D） technique. The purpose is to study the influence of DBD processing on the nature and scale of BSA adsorption on PMMA surface in vitro. It was observed that DBD processing improves the surface wettability of PMMA film, a fact attributable to the changes in surface chemistry and topography. Exposure of the PMMA to Phosphate Buffed Saline （PBS） solution in the QCM-D system resulted in surface adsorption which reaches an equilibrium after about 30 minutes for pristine PMMA, and 90 minutes for processed PMMA surface. Subsequent injection of BSA in PBS indicated that the protein is immediately adsorbed onto the PMMA surface. It was revealed that adsorption behaviour of BSA on pristine PMMA differs from that on processed PMMA surface. A slower adsorption kinetics was observed for pristine PMMA surface, whilst a quick adsorption kinetics for processed PMMA. Moreover, the dissipation shift of protein adsorption suggested that BSA forms a more rigid structure on pristine PMMA surface that on processed surface. These data suggest that changes in wettability and attendant chemical properties and surface texture of the PMMA surface may play a significant role in BSA adsorption process.

关键词： in vitro test protein adsorption surface modification polymethylmethacrylate QCM-D

Crystalline compounds for remediation of rare-earth fission products:A review

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journal of Rare Earths 2022年第3期40卷 365-380,I0001页

作者： Saehwa Chong brian j.Riley Zayne j.Nelson Pacific Northwest National Laboratory RichlandWA99352USA

Rare earth(RE)-containing crystals have been investigated as media for the immobilization of RE fission products from nuclear waste *** reprocessing of spent nuclear fuel,fission products including REs,alkalis,and alkaline earths released from the *** viable option to immobilize the RE fission products is to incorporate them into chemically durable crystalline phases in specific waste *** study summarizes the crystal structures and synthesis methods of six RE-containing compounds that have applications in remediation of RE fission *** compounds include oxyapatite(AE_(2)RE_(8)(SiO_(4))_(6)O_(2)),oxychloride(REOCl),borosilicate(RE_(3)BSi_(2)O_(10)),pyrochlore(RE_(2)M_(2)O_(7)),monazite(REPO_(4)),and perovskite(REMO_(3))where AE denotes alkaline earth metals and M denotes transition *** review provides an overview of literature on the usage of these six compounds for immobilizing RE fission products and summarizes different synthesis methods for producing these *** of structural parameters with different REs in each compound are also discussed.

关键词： Rare-earth compounds Nuclear waste forms Crystal structures Synthesis of rare-earth compounds

Trees are rarely most abundant where they grow best

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journal of Plant Ecology 2012年第1期5卷 46-51页

作者： brian j.McGill School of Biology and Ecology and Sustainability Solutions Initiative 303 Deering HallUniversity of MaineOronoME 04469USA

Aims A common assumption in ecology is that where a species is found to be most abundant must correspond to the environmental context in which the species performs the best(*** niche space).This assumption is central to common conservation and management tools such as habitat suitability assessment and species distribution modeling.I test this *** I use the US Forest Inventory Assessment data for the abundance of trees across eastern North America.I use the FORAST tree-ring dataset for ontogenetic growth rate(tree-ring increment),a measure of niche performance and correlated with intrinsic rate of increase,*** Findings I find that across 15 species,there are significantly more negative correlations than expected by *** negative correlation between abundance and performance across space contradicts common assumptions but is consistent with an inclusive niche structuring of the community.

关键词： tree rings biogeography inclusive niche climate change