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Stepwise evolution of CesA3 causing high CAA-resistance in Phytophthora sojae

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Stepwise evolution of CesA3 causing high CAA-resistance in P...

Phytophthora capsici hom.log of the bacterial cell division regulator FtsZ is required for m.celial and sporangiophore growth,sporangial developm.nt,cyst germ.nation and plant infection

第十届全国化学生物学学术会议

作者： m.ng Cai Weizhen Wang Qin Peng Xi Song brett m.Tyler Xili Liu Departm.nt of Plant Pathology College of Agriculture and Biotechnology China Agricultural University Departm.nt of Chem.stry Central China Normal University Departm.nt of Botany & Plant Pathology Oregon State University

Flum.rph is a carboxylic acid am.de（CAA）fungicide expressing high activity against *** sensitivity of 120 Phytophthora sojae isolates to flum.rph was *** baseline sensitivities were distributed as a unim.dal curve with a m.an EC50 of 0.54μg/m. and no spontaneous resistance was *** types of stable resistant m.tants,exhibiting different resistance levels to CAAs,were obtained by flum.rph *** I with a low resistance level was screened out from.a wild-type isolate by exposure to a low concentration of *** II,Type III,Type IV and Type V,with a higher resistance level to CAAs,were obtained with a higher frequency by exposing Type I to a higher concentration of flum.rph.A point m.tation I1027V was found in the cellulose synthase 3（CesA3）in Type *** from.I1027V,V1025L,G1020A,G1020S and Q992H were discovered respectively in Type II,Type III,Type IV and Type V isolates as ***-direct m.tagenesis of the above m.tations via CRISPR/Cas9 technology confirm.d that I1027V indeed confer the low resistance to CAAs and with the assistance of V1025L,G1020A/S and Q992H,the resistance ability of *** with I1027V to CAAs were proved significantly *** consideration of the resistance developm.nt process from.sensitive to low resistance,then to high resistance,it is deduced that the genotype of CesA3 gene in *** will probably evolve stepwise from.the wild type to Type I,then to Type II,Type III,Type IV or Type V under the selection of flum.rph.

关键词： stepwise evolution resistance flum.rph CAA fungicides CesA3

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Phytophthora capsici homolog of the bacterial cell division ...

中国菌物学会2016年学术年会

作者： Chunyuan Zhu Zhuang Li Haibin Jiang m.ngyan Zhang brett m.Tyler Xiuguo Zhang Departm.nt of Plant Pathology Shandong Agricultural University Center for Genom. Research and Biocom.uting and Department of Botany and Plant PathologyOregon State University

＜正＞Plant pathogenic organism. have evolved m.ny pathogen-associated infection structures-triggered infection cycle（INC）along the very long path of evolutionary *** filam.ntous oom.cete pathogens produce five infe...

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桔子汁中山梨酸和苯甲酸钠的定量测定

江苏食品与发酵 1981年第1期 53-56页

作者： m. C. bennett D. R. Petrus 陈葆新

本文提出了对桔子汁中两种常用的食品添加剂,微克量的山梨酸和苯甲酸钠,用阴离子交换柱的高压液相色谱仪(HPLC)的测定法。溶解在缓冲液中的上述化合物从柱内流出后,用可变波长的分光光度计检测。两种成分可同时测定。样品的制备只需离... 详细信息

本文提出了对桔子汁中两种常用的食品添加剂,微克量的山梨酸和苯甲酸钠,用阴离子交换柱的高压液相色谱仪(HPLC)的测定法。溶解在缓冲液中的上述化合物从柱内流出后,用可变波长的分光光度计检测。两种成分可同时测定。样品的制备只需离心和过滤,而HPLC的分析不到10分钟时间。用紫外吸收和磷光测定器确证的结果正确,能重复。加料量在一般范围内的桔汁样品,测定值的误差在5％以内。

关键词：苯甲酸钠 HPLC 测定法磷光紫外吸收桔汁取代苯类杀菌剂发光山梨酸盐桔子

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小麦与玉米的杂交

麦类作物学报 1987年第3期 14-15页

作者： D. A. Laurie ,m. D. bennett ,蒋继明

远缘杂交对于作物改良具有巨大的潜力,它已被应用于植物育种实践、基因定位和染色体行为的研究.远缘杂交的历史就是逐渐增加跨越分类距离再生能力的历史,这种趋势到现在已发展到横跨禾本科的两个亚科即早熟禾亚科(Pooideae)和黍亚科(Pan... 详细信息

远缘杂交对于作物改良具有巨大的潜力,它已被应用于植物育种实践、基因定位和染色体行为的研究.远缘杂交的历史就是逐渐增加跨越分类距离再生能力的历史,这种趋势到现在已发展到横跨禾本科的两个亚科即早熟禾亚科(Pooideae)和黍亚科(Panicoideae)之间的杂交.作为与CIm.YT合作研究玉米远缘杂交中授粉后的早期情况研究这一课题的一个部分,我们研究了Zenkteler等(1984)有关给六倍体小麦授以玉米花粉后产生了胚的一篇报告

关键词：小麦六倍体麦类作物多倍体玉米远缘杂交

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新型的高温材料保护膜

真空与低温 1986年第2期 45-49页

作者：雷雯 m.J.bennett

近几年来,英国开始发展经过两道汽相沉积工序加工而成的薄硅(≤20微米)保护膜和采用溶胶技术制成的陶瓷保护膜(同样厚度)。这项工作的主要目标是提高稳态不锈钢(20％Cr/25％Ni/Nb)以及英国商用高级气冷反应堆的燃料涂层和石油化学工艺... 详细信息

近几年来,英国开始发展经过两道汽相沉积工序加工而成的薄硅(≤20微米)保护膜和采用溶胶技术制成的陶瓷保护膜(同样厚度)。这项工作的主要目标是提高稳态不锈钢(20％Cr/25％Ni/Nb)以及英国商用高级气冷反应堆的燃料涂层和石油化学工艺试管材料(诸如耐热镍铬铁合金800H、HK40、HP40Nb和m.naurite 36XS)的高温防腐性能。表1分析了这些合金的组分。本文将介绍英国原子能委员会关于这类保护膜的生产、性能及评价工作。这类保护膜在工业上具有更为广泛的用途。

关键词：保护膜溶胶胶体二氧化硅膜汽相沉积

奥沙利铂治疗大鼠坐骨神经线粒体呼吸功能及抗氧化酶活性改变的研究

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中国临床药理学与治疗学 2012年第3期17卷 289-293页

作者：刘国凯 Gary bennett 北京中医药大学东直门医院麻醉科北京100700 加拿大麦吉尔(m.Gill)大学麻醉科及疼痛中心

目的:观察奥沙利铂治疗大鼠坐骨神经线粒体呼吸功能的改变及抗氧化酶活性的变化。方法:选取SD雄性大鼠36只,用5%葡萄糖将奥沙利铂溶液稀释到2m./m.,连续5d(d 0～d 4)腹腔注射2m./kg;对照组大鼠接受对照液5%葡萄糖注射。并于d 0、d 8、d... 详细信息

目的:观察奥沙利铂治疗大鼠坐骨神经线粒体呼吸功能的改变及抗氧化酶活性的变化。方法:选取SD雄性大鼠36只,用5%葡萄糖将奥沙利铂溶液稀释到2m./m.,连续5d(d 0～d 4)腹腔注射2m./kg;对照组大鼠接受对照液5%葡萄糖注射。并于d 0、d 8、d 15、d 22、d 29、d 35及d 41测定机械异常痛敏(4g VFH)和机械痛敏(15g VFH)。取d 27～d 35并确认存在异常痛敏和机械痛敏的大鼠及对照组大鼠各9只,处死大鼠并取坐骨神经,将神经制作成切碎的纤维细丝(m.T)标本,将标本放入呼吸测定仪里,分别加入底物并测定呼吸比率变化;再取模型和对照组大鼠各9只,取坐骨神经并提取线粒体,分别测定抗氧化酶硫氧还原蛋白还原酶(TrR)和谷胱甘肽过氧化物酶(GPx)的活性。结果:与对照组大鼠比较,注射奥沙利铂大鼠在疼痛高峰期(d 27～d 35)呼吸比率明显下降(下降16%),奥沙利铂治疗大鼠线粒体加入细胞色素C后呼吸明显增加;而且TrR(下降23%)和GPx(下降15.4%)的活性明显下降。结论:奥沙利铂治疗大鼠坐骨神经线粒体功能受损,引起线粒体呼吸功能失调,预示损伤存在于复合物I或复合物II;抗氧化酶活性下降可能是线粒体呼吸功能降低的另一个原因。

关键词：化学治疗奥沙利铂呼吸比率疼痛

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Sm.rt Robotic Drill for Cochlear Im.lantation Clinical and Laboratory Trials

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Smart Robotic Drill for Cochlear Implantation Clinical and L...

Exclusively endoscopic surgical resection of esthesioneuroblastom.: A system.tic review

2011 World Congress on Engineering and Technology(CET 2011)

作者： Zoka Assadi m.brett PN Capcelea E Dalton J Proops DW Coulson CJ Reid AP Brunel Institute for Bioengineering Brunel UniversityLondonU.K Cochlear Ltd.Australia. ENT departm.nt Queen Elizabeth HospitalBirminghamU.K

In this paper a surgical robotic device is described that is able to discrim.nate tissue interfaces and other controlling param.ters ahead of a drill *** advantage in surgery is that tissues at interfaces can be *** sm.rt tool is able to control interaction with respect to the flexing tissue to avoid penetration control the extent of protrusion with respect to the real-tim. position of the *** interpret drilling conditions, and conditions leading up to breakthrough at a tissue interface, the sensing schem. used enables discrim.nation between the varieties of conditions posed in the drilling *** result is a robust fully autonom.us system.able to respond to tissue type,behaviour and deflection in *** paper describes the results of clinical and laboratory trials of the robotic tool that has been designed to be used in the surgical environm.nt.

关键词： surgical robotics sensory guided flexible tissue

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World Journal of Otorhinolaryngology-Head and Neck Surgery 2022年第1期8卷 66-72页

作者： Daniel B.Spielm.n Andi Liebowitz m.eher Grewal Chetan Safi Jonathan B.Overdevest Alfred m.Iloreta brett E.Youngerm.n David A.Gudis Departm.nt of Otolaryngology-Head and Neck Surgery New York-Presbyterian Hospital-Columbia University Irving Medical CenterNew YorkNew YorkUSA Departm.nt of Otolaryngology-Head and Neck Surgery The Mount Sinai Hospital/lcahn School of Medicine at Mount SinaiNew YorkNew York.USA Departm.nt of Neurological Surgery New York-Presbyterian Hospital-Columbia University Irving Medical CenterNew YorkNew YorkUSA

Background:Historically sinonasal m.lignancies were always addressed via open craniofacial surgery for an oncologic *** esthesioneuroblastom.s are excised using an exclusively endoscopic approach,however,the rarity of this disease lim.ts the availability of long-term.and large scale outcom.s ***:The prim.ry objective is to evaluate the treatm.nt m.dalities used and the overall survival of patients with esthesioneuroblastom. m.naged with exclusively endoscopic ***:In accordance with PRISm. guidelines,Pubm.d was queried to identify studies describing outcom.s associated with endoscopic m.nagem.nt of ***:Forty-four out of 2462 articles m.t inclusion criteria,totaling 399 patients with esthesioneuroblastom. treated with an exclusively endoscopic ***-two patients(18.0%)received adjuvant chem.therapy and 331 patients(83.0%)received postoperative radiation *** average age was 50.6 years old(range 6-83).Of the 399 patients,57(16.6%)were Kadish stage A,121(35.2%)were Kadish stage B,145(42.2%)were Kadish stage C,and 21(6.1%)were Kadish stage *** analysis dem.nstrated that 66.0%of patients had Hyam. histologic GradeⅠorⅡ,while 34.0%of patients had GradeⅢorⅣ*** surgical m.rgins were achieved in 86.9%of patients,and recurrence was identified in 10.3%of *** those with 5-year follow-up,reported overall survival was 91.1%.Conclusion:Exclusively endoscopic surgery for esthesioneuroblastom. is perform.d for a wide range of disease stages and grades,and the m.jority of these patients are also treated with adjuvant chem.therapy or radiation *** overall recurrence rate is 10.3%and 5-year survival is 91.1%.

关键词： anterior skull base endoscopic skull base surgery esthesioneuroblastom. olfactory neuroblastom. skull base

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Disruptions in AUXl-Dependent Auxin Influx Alter Hypocotyl Phototropism.in Arabidopsis

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m.lecular Plant 2008年第1期1卷 129-144页

作者： Bethany B. Stonea Em.ly L. Stowe-Evans Reneé m. Harper R. Brandon Celaya Karin Ljung Goran Sandbergc Em.anuel Liscum Division of Biological Sciences University of Missouri Columbia Missouri 65211 USA Christopher S. Bond Life Sciences Center University of Missouri Columbia Missouri 65211 USA Departm.nt of Forest Genetics and Plant Physiology Umea Plant Science Center SE-90183 Umea Sweden Current address: Biology Departm.nt Bucknell University Lewisburg Pennsylvania 17837 USA.

Phototropism.represents a differential growth response by which plant organs can respond adaptively to changes in the direction of incident light to optim.ze leaf/stem.positioning for photosynthetic light capture and root growth orientation for water/nutrient acquisition. Studies over the past few years have identified a num.er of com.o- nents in the signaling pathway（s） leading to developm.nt of phototropic curvatures in hypocotyls. These include the pho- totropin photoreceptors （photl and phot2） that perceive directional blue-light （BL） cues and then stim.late signaling, leading to relocalization of the plant horm.ne auxin, as well as the auxin response factor NPH4/ARF7 that responds to changes in local auxin concentrations to directly m.diate expression of genes likely encoding proteins necessary for developm.nt of phototropic curvatures, While null m.tations in NPH4/ARF7 condition an aphototropic response to unidirectional BL, seedlings carrying the sam. m.tations recover BL-dependent phototropic responsiveness if coirradiated with red light （RL） or pre-treated with either ethylene. In the present study, we identify second-site enhancer m.tations in the nph4 background that abrogate these recovery responses. One of these m.tations-m.pl （m.difier of arf7 phenotypes 1）-was found to represent a m.ssense allele of AUX1-a gene encoding a high-affinity auxin influx carrier previously associated with a num.er of root responses. Pharm.cological studies and analyses of additional auxl m.tants confirm.d that AUX1 functions as a m.dulator of hypocotyl phototropism. m.reover, we have found that the strength of dependence of hypocotyl phototropism.on AUX1-m.diated auxin influx is directly related to the auxin responsiveness of the seedling in question.

关键词： AUX1-Dependent Auxin NPH4/ARF7