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A reproducible and sensitive method for generating high-quality transcriptomes from single whitefly salivary glands and other low-input tissues

1977年

作者：（加）j.r.小博尔德等

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Insect Science 2022年第5期29卷 1318-1328页

作者： Marco Gebiola brandon H.Le Kerry EMauck Department of Entomology University of California RiversideRiversideCalifornia Department of Botany and Plant Sciences University of California RiversideRiversideCalifornia Institute of Integrative Genome Biology University of California RiversideRiversideCaliforniaUSA

Transcriptomic studies are an important tool for understanding the molecular pathways underlying host plant use by agricultural pests,including vectors of damaging plant *** far,bulk rNA-Seq has been the main approach for non-model *** method relies on pooling large numbers of whole organisms or hundreds of individually dissected *** latter approach is logistically challenging,may introduce artifacts of handling and storage,and is not compatible with biological ***,we tested an approach to generate transcriptomes of individual salivary glands and other low-input body tissues from whiteflies(Bemisia tabaci MEAM1),which are maj.r.vectors of plant *** comparing our outputs to published bulk rNA-Seq datasets for whole whitefly bodies and pools of salivary glands,we demonstrate that this approach recovers similar numbers of transcripts relative to bulk rNA-Seq in a tissue-specific manner,and for some metrics,exceeds performance of bulk tissue *** generated from individual salivary glands also yielded additional novel transcripts not identified in pooled salivary gland datasets,and had hundreds of enriched transcripts when compared with whole head ***,our study demonstrates that it is feasible to produce high quality,replicated transcriptomes of whitefly salivary glands and other low-input *** anticipate that our approach will expand hypothesis-driven research on salivary glands of whiteflies and other Hemiptera,thus enabling novel control strategies to disrupt feeding and virus transmission.

关键词： Aleyrodidae Bemisia tabaci bulk rNA-Seq insect vectors low-input rNA-Seq plant pathogens

Mapping Sea Level rise Behavior in an Estuarine Delta System：A Case Study along the Shanghai Coast

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Engineering 2018年第1期4卷 156-163页

作者： H.Q. Cheng j.Y. Chen Z.j. Chen r.L. ruan G.Q. Xu G. Zeng j.r. Zhu Z.j. Dai X.Y. Chen S.H. Gu X.L. Zhang H.M. Wang State Key Laboratory of Estuarine and Coastal research East China Normal University Shanghai 200062 China Shanghai Water Planning Design and research Institute Shanghai 200232 China Shanghai Water Authority Shanghai 200050 China School of Urban and regional Science East China Normal University Shanghai 200062 China School of Ecological and Environmental Sciences East China Normal University Shanghai 200062 China Shanghai Hydrological Station Shanghai 200232 China Shanghai Municipal Bureau of Urban Planning and Land resources Shanghai 200003 China Shanghai Institute of Geological Survey Shanghai 200072 China

Sea level rise （SLr） is a maj.r.projected threat of climate change that is expected to affect developing coastal cities located in estuarine delta regions, Shanghai is one such city, being located in the Yangtze river Delta （YrD）, It is difficult, however, for decision-makers to implement adaptation due to the uncer- tain causes, magnitudes, and timings of SLr behaviors, This paper attempts to map the causes and mag- nitudes of SLr behaviors on a decadal scale, We analyze the tidal level records from 11 tidal gauge stations and the corresponding bathymetry measurements around these stations since 1921, We identify three new SLr behaviors along the Shanghai coast due to anthropogenic geomorphologic changes （AGCs）, besides the well-known eustatic sea level rise （ESLr）, tectonic subsidence （TS）, and urban land subsidence （ULS）, The first new behavior is regional sea level rise （rSLr）, which occurs as a result of land reclamation and deep waterway regulation, The second is regional sea level fall （rSLF）, which occurs because the channel bed is eroded due to sediment supply decline in the river catchment, The last SLr behavior is local tidal datum rise （LTDr）. Thus, we project that the magnitude of SLr for the Shanghai coast ranges from 10 cm to 16 cm from 2011 to 2030, Clarifying SLr behaviors is important to aid local decision- makers in planning structural and non-structural measures to combat escalating flood damage costs in an estuarine delta system; this field is full of future challenges,

关键词： Sea level rise behavior Anthropogenic geomorphologic change Local tidal datum Flood management Adaptation

MICrOSTrUCTUrES AND OXIDATION rESISTANCE OF Fe_3Al WELD OVErLAY

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Acta Metallurgica Sinica(English Letters) 2001年第4期14卷 253-256页

作者： X.G. Mi X.Q. Yu, Y.S. Sun and j.r. Sun Department of Materials Science and Engineering, Southeast University, Nanjing 210096, China Dept. of Mat. Sci. Southeast Univ. Nanjing 210096 China

Using the Fe3Al electrode through manual arc surfacing (MAS), Fe3Al coatings were deposited on the stainless steel substrate. The microstructures, hardness and oxidation resistance of the weld overlay were investigated. The results show that crack-free overlays can be obtained when pre-heating the substrate at 500°C and postweld heat treatment at 700°C are used. Elements of Al, Cr and Ni, etc. transfer between the substrate and the overlay, but this does not influence the microhardness of the substrate and the Fe3Al overlay. Oxidation tests show that the Fe3Al overlay has excellent oxidation resistance in comparison with the stainless steel substrate at 800°C and 900°C.

关键词： Aluminum Intermetallics Iron Microstructure Oxidation resistance

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工业等离子体工程

Microstructure, precipitates and mechanical properties of powder bed fused inconel 718 before and after heat treatment

作者： j.r.罗思

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journal of Materials Science & Technology 2019年第6期35卷 1153-1164页

作者： Le Zhou Abhishek Mehta brandon McWilliams Kyu Cho Yongho Sohn Advanced Materials Processing and Analysis Center University of Central Florida Orlando FL 32816 USA Department of Materials Science and Engineering University of Central Florida Orlando FL 32816 USA Weapons and Materials research Directorate US. Amy Research Laboratory Aberdeen Proving Ground MD 21005 USA

IN718 alloy was fabricated by laser powder bed fusion(PBF) for examination of microstructure, precipitates and mechanical properties in the as-built state and after different heat treatments. The as-built alloy had a characteristic fine cellular-dendritic microstructure with Nb, Mo and Ti segregated along the interdendritic region and cellular boundary. The as-built alloys were then subjected to solution heat treatment(SHT) at 980°C or 1065°C for 1 h. SHT at 980°C led to the formation of δ-phase in the interdendritic region or cellular boundary. The segregation was completely removed by the SHT at 1065°C, but recrystallization was observed, and the carbides decorated along the grain boundaries. The as-built alloy and alloys with SHT at 980°C and 1065°C were two-step aged, which consisted of annealing at 720°C for8 h followed by annealing at 620°C for 8 h. Transmission electron microscopy revealed the precipitation of λ’ and λ" in all alloys after two-step aging, but the amount and uniformity of distribution varied. The Vickers hardness of the PBF IN718 alloy increased from 296 HV to 467 HV after direct aging. The hardness decreased to 267 HV and 235 HV after SHT at 980°C and 1065°C, respectively, but increased to 458 HV and 477 HV followed by aging. The evolution of Young’s modulus after heat treatment exhibited similar trend to that of hardness. The highest hardness was observed for IN718 after SHT at 1065°C and two-step aging due to precipitation with greater amount and uniform distribution.

关键词： Laser powder bed fusion IN718 Microstructure Transmission electron microscopy

Treating amyotrophic lateral sclerosis with allogeneic Schwann cell-derived exosomal vesicles: a case report

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Neural regeneration research 2025年第4期20卷 1207-1216页

作者： Pascal j.Goldschmidt-Clermont Aisha Khan George jimsheleishvili Patricia Graham Adriana Brooks risset Silvera Alexander j.P.Goldschmidt Damien D.Pearse W.Dalton Dietrich Allan D.Levi james D.Guest Miller School of Medicine University of MiamiMiamiFLUSA Stem Cell Institute Miller School of MedicineUniversity of MiamiMiamiFLUSA The Miami Project to Cure Paralysis Miller School of MedicineUniversity of MiamiMiamiFLUSA Alzady International LLC MiamiFLUSA Neurological Surgery Miller School of MedicineUniversity of MiamiMiamiFLUSA

Schwann cells are essential for the maintenance and function of motor neurons,axonal networks,and the neuromuscular *** amyotrophic lateral sclerosis,where motor neuron function is progressively lost,Schwann cell function may also be ***,important signaling and potential trophic activities of Schwann cell-derived exosomal vesicles have been *** case report describes the treatment of a patient with advanced amyotrophic lateral sclerosis using serial intravenous infusions of allogeneic Schwann cell-derived exosomal vesicles,marking,to our knowledge,the first instance of such *** 81-year-old male patient presented with a 1.5-year history of rapidly progressive amyotrophic lateral *** initial diagnosis,the patient underwent a combination of generic riluzole,sodium phenylbutyrate for the treatment of amyotrophic lateral sclerosis,and *** patient volunteered to participate in an FDA-approved single-patient expanded access treatment and received weekly intravenous infusions of allogeneic Schwann cell-derived exosomal vesicles to potentially restore impaired Schwann cell and motor neuron *** confirmed that cultured Schwann cells obtained from the amyotrophic lateral sclerosis patient via sural nerve biopsy appeared impaired(senescent)and that exposure of the patient’s Schwann cells to allogeneic Schwann cell-derived exosomal vesicles,cultured expanded from a cadaver donor improved their growth capacity in *** a period of observation lasting 10 weeks,during which amyotrophic lateral sclerosis Functional rating Scale-revised and pulmonary function were regularly monitored,the patient received weekly consecutive infusions of 1.54×1012(×2),and then consecutive infusions of 7.5×1012(×6)allogeneic Schwann cell-derived exosomal vesicles diluted in 40 mL of Dulbecco’s phosphate-buffered *** of the infusions were associated with adverse events such as infusion reactions(allergic or otherwise)or

关键词： allogeneic amyotrophic lateral sclerosis exosomes infusion neuromuscular junction Schwann cell

radiofrequency electric-field heating behaviors of highly enriched semiconducting and metallic single-walled carbon nanotubes

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Nano research 2015年第9期8卷 2859-2870页

作者： Stuart j. Corr Mustafa raoof brandon T. Cisneros Alvin W. Orbaek Matthew A. Cheney justin j. Law Nadia C. Lara Andrew r. Barron Lon j. Wilsor Steven A. Curley Division of Surgery Baylor College of Medicine Houston TX 77030 USA Department of Surgical Oncology University of Texas M.D. Anderson Cancer Center Houston TX 77030 USA Department of Chemistry and the richard E. Smalley Institute for Nanoscale Science and Technology Rice University Houston TX 77005 USA Department of Surgery The University of Arizona College of Medicine Tucson AZ 85724 USA Department of Chemistry and Biochemistry University of California San Diego La Jolla CA 92093 USA College of Engineering Swansea University Singleton Park Swansea SA2 8PP Wales UK Department of Mechanical Engineering and Materials Science Rice University Houston TX 77005 USA

It is theorized that enhanced thermal heating may result from exposing single-walled carbon nanotubes （SWNTs） embedded in a conductive host to radiofrequency （rF） electric fields. We examine the rF-induced （13.56 MHz） heating behaviors of 95% metallic- and semiconducting-enriched SWNTs （m-/s-SWNTs） suspended in aqueous solutions with varying NaC1 molarity （0.001 mM-1 M）. The heating effects were only evident for host molarities below 1 mM （equivalent to 0.1 S/m） at which the s-SWNT heating rates dominated those of the m-SWNTs. The heating effects were localized to aligned and aggregated ＂SWNT ropes＂ -1 cm in length that formed in suspension, parallel to the electric-field vector, during the rF exposure. For molarities above 1 mM, no enhancements were evident, owing to the large heating effects of the bulk ionic NaC1 suspensions, which were observed in previous studies. Although larger enhancement effects proportional to the host conductivity have been theoretically predicted for m-/s-SWNT suspensions, this was not observed most likely because of the aggregation and screening effects, which diminished the scattered electric field near the m-/s-SWNTs. Our research may further the development of better nanoparticle heating agents for applications such as non-invasive rF-induced cancer hyperthermia.

关键词： rad.iofrequency,carbon nanotubes,metallic,semiconducting,hyperthermia

Myalgia may not be associated with severity of coronavirus disease 2019 (COVID-19)

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World journal of Emergency Medicine 2020年第3期11卷 193-194页

作者： Giuseppe Lippi johnny Wong brandon Michael Henry Section of Clinical Biochemistry Department of NeuroscienceBiomedicine and MovementUniversity of VeronaVeronaItaly College of Medicine SUNY Downstate Health Sciences UniversityNew YorkUSA Cardiac Intensive Care Unit The Heart InstituteCincinnati Children’s Hospital Medical CenterOhioUSA

Dear editor,A recent study based on the use of experimental artificial intelligence(AI)tool showed 70%–80%accuracy in predicting development of severe disease in coronavirus disease 2019(COVID-19)based on predictive parameters alanine aminotransferase(ALT),myalgia and hemoglobin,whilst only 5 of 53 patients developed acute respiratory distress syndrome(ArDS),2 of whom reporting myalgia.

关键词： distress severity acute

joint Ofshore Wind and Wave Energy resources in the Caribbean Sea

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journal of Marine Science and Application 2021年第4期20卷 660-669页

作者： brandon j.Bethel School of Marine Sciences Nanjing University of Information Science&TechnologyNanjing 210044China

Complementarities between wind and wave energies have many signifcant advantages that are unavailable with the sole deployment of *** all available wind speed,signifcant wave height,and wave period buoy observations over a 10-year period(i.e.,2009–2019),colocated wind and wave energy resources are *** buoy records are imper-fect,results show that the inner Caribbean Sea(CS)under the infuence of the Caribbean low-level jet has the highest wind energy resource at~1500 W/m^(2),followed by the outer CS at~600 W/m^(2) and Atlantic Ocean(AO)at~550–600 W/m^(2) at a 100 m *** energy was most abundant in the AO at 14 kW/m,followed by the inner CS at 13 kW/m and outer CS at 5 kW/*** average and dominant wave energies can reach a maximum of 10 and 14 kW/m,*** between wind and wave energy resources is observed in the AO,where wave energy is higher than the low wind speed/energy would *** is responsible for this discrepancy;thus,it must be considered not only for wave energy extraction but also for wind turbine fatigue,stability,and power extraction efciency.

关键词： Wave energy Wind energy Colocation Caribbean Sea Small island developing states NDBC buoys