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Impacts of dietary supplementation of bamboo vinegar and charcoal powder on growth performance,intestinal morphology,and gut microflora of large-scale loach Paramisgurnus dabryanus

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Journal of Oceanology and Limnology 2023年第3期41卷 1187-1196页

作者： Kwangjin ju Myongsuk KIL Sanghyok RI Tongchol kim juhyok kim Wei SHI Lining ZHANG Maocang YAN Jiongming ZHANG Guangxu LIU College of Animal Sciences Zhejiang UniversityHangzhou 310058China College of Aquaculture Wonsan Fisheries UniversityWonsan 999093DPR Korea College of Life Science Kim Hyong Jik University of EducationPyongyang 99903DPR Korea Zhejiang Mariculture Research Institute Wenzhou 325005China

Although accumulating data demonstrate that dietary supplementation of bamboo vinegar and charcoal powder(BVC)can significantly strengthen the immunity and boost the growth of domestic animals,its application potency still awaits verification in commercial fish species such as the large-scale loach Paramisgurnus *** impacts of 90-day dietary supplementation of 1%and 2%BVC on survival and growth performance of the loach,and on the intestinal morphological characteristics and gut microflora were *** data show that the large-scale loach supplied with BVC at the experimental doses had significant higher survival rates and better growth performance(indicated by greater weight gain(1.13–1.14 times),higher specific growth rate(1.04 times),and lower feed conversion ratio(0.88–0.89 times))compared to that of the control(P<0.05).Histological examination revealed significant longer villus(3.22–5.54 times),deeper crypt(1.77–1.87 times),and thicker muscle(1.59–3.17 times)in the intestines of large-scale loach fed with BVC(P<0.05).Furthermore,we found that the gut microflora consisted of significantly fewer proportions of potential pathogenic bacterial species(Aeromonas veronii and Escherichia coli),but significantly greater proportions of beneficial microbes(Lactococus raffinolactis and Faecalibacterium prausnitzii).Therefore,dietary intake of BVC can promote intestinal tract development and optimize gut microflora,by which the survival and growth of large-scale loach may be improved.

关键词： bamboo vinegar bamboo charcoal Paramisgurnus dabryanus growth performance intestinal morphology gut microflora

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Sphingosine-1-phosphate hinders the osteogenic differentiation of dental pulp stem cells in association with AKT signaling pathways

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International Journal of Oral Science 2022年第2期14卷 192-200页

作者： bongkun Choi Ji-Eun kim Si-On Park Eun-Young kim Soyoon Oh Hyuksu Choi Dohee Yoon Hyo-Jin Min Hyung-Ryong kim Eun-ju Chang Department of Biomedical Sciences Asan Medical CenterUniversity of Ulsan College of MedicineSeoulKorea Stem Cell Immunomodulation Research Center Asan Medical CenterUniversity of Ulsan College of MedicineSeoulKorea Department of Pharmacology College of DentistryJeonbuk National UniversityJeonjuKorea Department of Biochemistry and Molecular Biology Asan Medical CenterUniversity of Ulsan College of MedicineSeoulKorea

Sphingosine-1-phosphate(S1P)is an important lipid mediator that regulates a diverse range of intracellular cell signaling pathways that are relevant to tissue engineering and regenerative ***,the precise function of S1P in dental pulp stem cells(DPSCs)and its osteogenic differentiation remains *** here investigated the function of S1P/S1P receptor(S1PR)-mediated cellular signaling in the osteogenic differentiation of DPSCs and clarified the fundamental signaling *** results showed that S1P-treated DPSCs exhibited a low rate of differentiation toward the osteogenic phenotype in association with a marked reduction in osteogenesis-related gene expression and AKT *** note,both S1PR1/S1PR3 and S1PR2 agonists significantly downregulated the expression of osteogenic genes and suppressed AKT activation,resulting in an attenuated osteogenic capacity of *** importantly,an AKT activator completely abrogated the S1P-mediated downregulation of osteoblastic markers and partially prevented S1P-mediated attenuation effects during ***,the pro-inflammatory TNF-αcytokine promoted the infiltration of macrophages toward DPSCs and induced S1P production in both DPSCs and *** findings indicate that the elevation of S1P under inflammatory conditions suppresses the osteogenic capacity of the DPSCs responsible for regenerative endodontics.

关键词： pulp hinder precise

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A New Infrared Atmospheric Sounding Interferometer Channel Selection and Assessment of Its Impact on Met Office NWP Forecasts

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Advances in Atmospheric Sciences 2017年第11期34卷 1265-1281页

作者： Young-Chan NOH Byung-ju SOHN. Yoonjae kim Sangwon JOO William BELL Roger SAUNDERS School of Earth and Environmental Sciences Seoul National University Seou108826 Korea Numerical Modeling Center Korea Meteorological Administration Seou107062 Korea Met Office Exeter EXI 3PB United Kingdom

A new set of Infrared Atmospheric Sounding Interferometer （IASI） channels was re-selected from 314 EUMETSAT channels. In selecting channels, we calculated the impact of the individually added channel on the improvement in the analysis outputs from a one-dimensional variational analysis （1D-Var） for the Unified Model （UM） data assimilation system at the Met Office, using the channel score index （CSI） as a figure of merit. Then, 200 channels were selected in order by counting each individual channel＇s CSI contribution. Compared with the operationally used 183 channels for the UM at the Met Office, the new set shares 149 channels, while the other 51 channels are new. Also examined is the selection from the entropy reduction method with the same 1D-Var approach, Results suggest that channel selection can be made in a more objective fashion using the proposed CSI method. This is because the most important channels can be selected across the whole IASI observation spectrum. In the experimental trial runs using the UM global assimilation system, the new channels had an overall neutral impact in terms of improvement in forecasts, as compared with results from the operational channels. However, upper-tropospheric moist biases shown in the control run with operational channels were significantly reduced in the experimental trial with the newly selected channels. The reduction of moist biases was mainly due to the additional water vapor channels, which are sensitive to the upper-tropospheric water vapor.

关键词： Hyperspectral IR sounding channel selection 1D-Vat data assimilation

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Visceral hypersensitivity and altered colonic motility after subsidence of inflammation in a rat model of colitis

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World Journal of Gastroenterology 2003年第12期9卷 2791-2795页

作者： jun-Ho La Tae-Wan kim Tae-Sik Sung Jeoung-Woo Kang Hyun-ju kim ⅠI-Suk Yang Department of Physiology College of Veterinary MedicineSeoul National UniversitySeoulRepublic of Korea

AIM:Irritable bowel syndrome(IBS)is a functional bowel disorder characterized by visceral hypersensitivity and altered bowel *** is increasing evidence suggesting the role of inflammation in the pathogenesis of IBS,which addresses the possibility that formerly established rat model of colitis could be used as an IBS model after the inflammation subsided. METHODS:Colitis was induced by intracolonic instillation of 4% acetic acid in male Sprague-Dawley *** extent of inflammation was assessed by histological examination and myeloperoxidase(MPO)activity *** subsidence of colitis,the rats were subjected to rectal distension and restraint stress,then the abdominal withdrawal reflex and the number of stress-induced fecal output were measured, respectively. RESULTS:At 2 days post-induction of colitis,the colon showed characteristic inflammatory changes in histology and 8-fold increase in MPO *** 7 days post-induction of colitis,the histological features and MPO activity returned to *** rats at 7 days post-induction of colitis showed hypersensitive response to rectal distension without an accompaning change in rectal compliance,and defecated more stools than control animals when under ***: These results concur largely with the characteristic features of IBS, visceral hypersensitivity and altered defecation pattern in the absence of detectable disease, suggesting that this animal model is a methodologically convenient and useful model for studying a subset of IBS.

关键词： Acetic Acid Animals Biological Markers Colitis Disease Models, Animal Inflammation Irritable Bowel Syndrome Male Pain Peroxidase Rats Rats, Sprague-Dawley Research Support, Non-U.S. Gov't

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Spatial Control of Lithium Deposition by Controlling the Lithiophilicity with Copper(Ⅰ)Oxide Boundaries

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Energy & Environmental Materials 2023年第5期6卷 183-191页

作者： ju Ye kim Oh B.Chae Gukbo kim Woo-Bin jung Sungho Choi Do Youb kim San Moon jungdon Suk Yongku Kang Mihye Wu Hee-Tae jung Department of Chemical and Biomolecular Engineering(BK-21 four)and Institute for Nanocentury Korea Advanced Institute of Science and Technology(KAIST)DaejeonKorea Chemical&Process Technology Division Korea Research Institute of Chemical TechnologyDaejeon 34114Korea School of Engineering Brown UniversityProvidence 02912USA Department of Chemistry University of Rhode IslandKingston 02881USA School of Engineering and Applied Sciences Harvard UniversityCambridge 02138USA Advanced Materials Division Korea Research Institute of Chemical TechnologyDaejeon 34114Korea

Spatial control of lithium deposition is the most important issue in lithium-metal batteries because of the considerable control of lithium dendrite suppression via the uniform distribution of Li^(+)*** seed materials are crucial for the behavior of lithium deposition,in-depth studies on their physical and chemical control have not been ***,we describe a new design of seed structure comprising a wrinkled Cu/graphene substrate surrounded by copper(Ⅰ)oxide(Cu_(2)O)on a graphene grain boundary over a large area,which is fabricated by the oxidation of the Cu surface via graphene boundary defects by using chemical vapor deposition(CVD).Scanning and transmission electron microscopy results reveal that Cu_(2)O on the graphene boundary can render a preferential reaction with lithium during the first deposition and assist in the uniform deposition of lithium by preventing the agglomeration of lithium clusters during the second *** two-step process is attributed to the degree of selectivity due to the difference in lithium affinity,which allows long-term electrochemical stability and a high rate capability via boundary *** study highlights the significance of the boundary effect,which can open new avenues for the formation of a large family of seed structures in lithium-metal batteries.

关键词： Cu_(2)O boundary dendrite-free lithium deposition lithium-metal battery lithium spatial control seed boundary effect

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Conceptual design of the Kumgang laser: a high-power coherent beam combination laser using SC-SBS-PCMs towards a Dream laser

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High Power Laser Science and Engineering 2015年第1期3卷 19-31页

作者： Hong Jin Kong Sangwoo Park Seongwoo Cha Heekyung Ahn Hwihyeong Lee jungsuk Oh bong ju Lee Soungwoong Choi Jom Sool kim Department of Physics Korea Advanced Institute of Science and Technology (KAIST) Department of Advanced Green Energy and Environment Handong Global University Laser Spectronix

In this review paper, we introduce a self-phase controlled stimulated Brillouin scattering phase conjugate mirror(SCSBS-PCM) and the Kumgang laser. The SC-SBS-PCM was proposed and demonstrated its success at the academic low power level, ~100 mJ@10 Hz. The Kumgang laser is under development to verify whether the SC-SBS-PCM is operable at the k W level. It is a 4 kW beam combination laser combining four 1 k W beams using the SC-SBS-PCM. If the Kumgang laser functions successfully, it will be the most important step towards a Dream laser, a hypothetical laser with unlimited power and a high repetition rate.

关键词： coherent beam combination diode-pumped laser high-power laser self-phase controlled SBS-PCM

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Biomass growth characteristics of 13-year-old Pinus densiflora *** *** a post-fire plantation on different contour conditions in Samcheuk,Korea

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Journal of Mountain Science 2016年第7期13卷 1238-1244页

作者： Lee ju-Hyoung Lee Do-Hyung kim Do-Hyun Park Jin-Hwa kimJae-Hee Department of Forest Resources Yeungnam UniversityGyeongsan 712-749Republic of Korea Useful Plant Resources Center Korea National ArboretumYangpgeong-gun 12519Republic of Korea Korea Forest Welfare Institute Therapy-ro 209Bonghyeon-myeonYeongju-siGyeongsangbuk-doRepublic of Korea Department of forest genetic resources National Institute of Forest Science57HoegiroDongdaemun-guSeoul 02455Republic of Korea

We used preliminary data to estimate the growth volume of artificially reforested Pinus densiflora in a post-fire area on three different contour conditions. We compared the growth of P. densiflora on a south-facing slope(Ssth), north-facing slope(Snth) and ridge area(Ridge), using 7 trees selected from each stand aspect. The tree height, diameter and growth volume were measured and the dry weight of each plant part were compared and analyzed. The results revealed that the total dry weight was highest on Ssth(5992.3 g), followed by Snth(4833.2 g) and lowest on Ridge(3160.1 g). The height growth was highest on Snth(285.8 cm), followed by Ssth(274.5 cm) and lowest on Ridge(211.5 cm). The diameter growth was greatest on Ssth(7.37 cm), followed by Snth(7.10 cm) and lowest on Ridge(5.72 cm). The volume growth was highest on Ssth(4257.7 cm3), followed by Snth(3750.7 cm3) and lowest on Ridge(2093.7 cm3). Therefore, we should consider and include the concept of slope orientation together with differences in habitat environments in afforestation projects when creating artificial forests with P. densiflora. These study results can serve as important preliminary data for future establishment of artificial forest of P. densiflora in a post-fire plantation.

关键词： Pinus densiflora Growth characteristics Contour conditions Planted stand Post-fire plantation

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Thoracic pyogenic infectious spondylitis presented as pneumothorax:A case report

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World Journal of Clinical Cases 2021年第6期9卷 1402-1407页

作者： Mi-Kyung Cho Byeong-ju Lee Jae-Hyeok Chang Young-Mo kim Department of Rehabilitation Medicine Pusan National University HospitalBusan 49241South Korea

BACKGROUND Pyogenic infectious spondylitis(PIS)is a rare condition,with an incidence between 0.2 and 2 cases per 100000 per ***’s most common symptom-back or neck pain-occurs in more than 90%of ***,we reported a case of thoracic PIS accompanied by pneumothorax in a 65-year-old male *** SUMMARY A 65-year-old man presented with right chest pain and *** initial erect posteroanterior chest radiography revealed pneumothorax,which was further evaluated by chest computed tomography,revealing pleural effusion in the right lung and a paravertebral abscess with bony destruction of vertebral *** on magnetic resonance imaging,the patient was diagnosed with thoracic infectious spondylitis with an anterior paravertebral *** was prescribed antibiotics and underwent neurosurgery due to aggravated symptoms and neurologic *** examination revealed that the cause of pleural effusion and pneumothorax was Staphylococcus aureus infection contiguously spread to lung *** several surgical treatments with intravenous antibiotic therapy for two months and transition to oral antibiotics(rifampin 600 mg qd and ciprofloxacin 500 mg bid),the patient received physical therapy to recover *** month after discharge,the patient had no chest pain or dyspnea,and exhibited no elevation in inflammatory markers or new thoracic *** To our knowledge,this is the very first report of a case of thoracic PIS with pneumothorax.

关键词： Chest pain Pneumothorax Pleural effusion Neurologic deficits Spondylitis Case report

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HOS1 Facilitates the Phytochrome B-Mediated Inhibition of PIF4 Function during Hypocotyl Growth in Arabidopsis

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Molecular Plant 2017年第2期10卷 274-284页

作者： ju-Heon kim Hyo-jun Lee Jae-Hoon jung Sangmin Lee Chung-Mo Park Department of Chemistry Seoul National University Seoul 08826 Korea Sainsbury Laboratory University of Cambridge Cambridge CB2 1LR UK Plant Genomics and Breeding Institute Seoul National University Seoul 08826 Korea These authors contributed equally to this article Present address: SK Innovation Daejeon 34124 Korea

Upon exposure to light, developing seedlings undergo photomorphogenesis, as illustrated by inhibition of hypocotyl elongation, cotyledon opening, and leaf greening. During hypocotyl photomorphogenesis, light signals are sensed by multiple photoreceptors, among which the red/far-red light-sensing phytochromes have been extensively studied. However, it is not fully understood how the phytochromes modulate hypo- cotyl growth. Here, we demonstrated that HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES 1 （HOS1）, which is known to either act as E3 ubiquitin ligase or affect chromatin organization, inhibits the transcriptional activation activity of PHYTOCHROME INTERACTING FACTOR 4 （PIF4）, a key transcrip- tion factor that promotes hypocotyl growth. Consistent with the negative regulatory role of HOSl in hypo- cotyl growth, HOSl-defective mutants exhibited elongated hypocotyls in the light. Notably, phyB induces HOS1 activity in inhibiting PIF4 function. Taken together, these observations provide a molecular basis for the phyB-mediated suppression of hypocotyl growth in Arabidopsis.

关键词： photomorphogenesis hypocotyl growth phytochrome signaling HOS1 PIF4

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Direct modification of spermatogonial stem cells using lentivirus vectors in vivo leads to efficient generation of transgenic rats

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Asian Journal of Andrology 2019年第2期21卷 190-195页

作者： Bang-Jin kim Yong-Hee kim Myeong-Geun Oh Ki-jung kim Sang-Eun jung ju-Hee Jin Sun-Uk kim Kwan-Sik Min Buom-Yong Ryu Department of Animal Scienee and Technology College of Biotechnology and Natural ResourcesChung-Ang UniversityAnseongGyeonggi-do 17546Korea National Primate Research Center Korea Research Institute of Bioscienee and BiotechnologyCheongjuChungcheongbuk-do 28116Korea Futuristic Animal Resource and Research Center Korea Research Institute of Bioscience and BiotechnologyCheongjuChungcheongbuk-do 28116Korea Animal Biotechnology Graduate School of Future Con verge nee Tech no logyDepartment of Animal Life ScienceInstitute of Genetic Engi neeringHankyong National UniversityAnseongGyeon ggi-do 17579Korea Department of Cancer Biology University of PennsylvaniaPhiladelphiaPA 19104USA.

Spermatogonial stem cells(SSCs)transmit genetic information to the next progeny in ***,SSCs are a potential target for germ I i ne modifications to gen erate tran sgenic an *** this study,we report a technique for the gen erati on of tran sgenic rats by in vivo manipulation of SSCs with a high success *** in juvenile rats were transduced in vivo with high titers of lentivirus harbori ng enhan ced green fluoresce nt protei n and mated with wild-type females to create foun der *** founder rats expressed the transgene and passed on the transgene with an overall success rate of 50.0%.Subsequent generations of progeny from the founder rats both expressed and passed on the ***,direct modification of SSCs in juvenile rats is an effective means of generating transgenic rats through the male *** technology could be adapted to larger animals,in which existing methods for gene modificatio n are in adequate or in applicable,resulti ng in the gen eration of tran sge nic an imals in a variety of species.

关键词： germline modification lentivirus spermatogonial stem cell testis transgenic animal

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