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Steroidal Plant Growth Promoters vs. Phytopathogens, via Enzymatic regulation;An in <i>Silico</i>Approach

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Advances in Enzyme research 2021年第4期9卷 55-71页

作者： Alan Carrasco-Carballo Emiliano Marín-Merino Penélope Merino-Montiel blanca Colin-Lozano Sandra Luz Cabrera Hilerio Jazmin Ciciolil Hilario-Martínez Jesús Sandoval-Ramírez Laboratorio de Elucidación y Síntesis en Química Orgánica Facultad de Ciencias Químicas Benemérita Universidad Autónoma de Puebla Puebla México Laboratorio de Síntesis y Modificación de Productos Naturales Facultad de Ciencias Químicas Benemérita Universidad Autónoma de Puebla Puebla México Laboratorio de Bromatología Facultad de Ciencias Químicas Benemérita Universidad Autónoma de Puebla Puebla México 4Facultad de Estudios Superiores-Zaragoza UNAM CDMX México Facultad de Estudios Superiores-Zaragoza UNAM CDMX México

Steroidal plant growth promoters (SPGP) have been continuously studied due to their high activity increasing biomass and resistance to diverse stress factors. In our hands, a new SPGP family of 22-oxocholestanic compounds stands out at a comparative level to brassinosteroids (BSs). The potential activity of new SPGP against phytopathogens was studied through in silico molecular docking, these assays were performed with relevant ensymes of phytopatogens Chitinase B and 1,3-β-Glucanase. Nine Chitinase B inhibitors and two 1,3-β-Glucanase inhibitors were proposed. The launched study analyzed the interactional and spatial level, determining the presence of interactions with key amino acids in receptors in comparison to reference inhibitors. Even more, the AVR4 and ECP6 effectors were also examined. No compound that blocks ECP6 was found;due to, probably, the influence of its highly hydrophilic environment. In the case of AVR4, two SPGP showed a better docking score (DS) than a chitin fragment (endogenous ligand);this fact demonstrates the latent potential of the 22-oxocholestanic derivatives against phytopathogens, with a specific regulation via proliferation inhibition. Moreover, this SPGP does not affect the symbiotic fungi that are beneficial for the natural plant system.

关键词： 22-Oxocholestanes Brassinosteroids Chitinase B 1 3-β-Glucanase ARV4 ECP6

<i>Stenotrophomonas maltophilia</i>Keratitis related to Therapeutic Contact Lens Misidentified with an Automated Identification System

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Open Journal of Clinical Diagnostics 2015年第2期5卷 63-67页

作者： Daniel Fatela-Cantillo Javier Crespo-González blanca Fatela-Cantillo Antonio Fernandez-Suarez Jose Miguel Aguilar Benítez Juan Antonio Saez Nieto remedios González-Amezcua Department of Biotechnology Hospital Alto Guadalquivir Andújar (Jaén) Spain Department of Ophthalmology Hospital Linares Linares (Jaén) Spain Department of Ophthalmology Hospital La Princesa Madrid (Madrid) Spain Department of Biotechnology Hospital Alcalá La Real Alcalá La Real (Jaén) Spain NationalMicrobiology Centre Majadahonda (Madrid) Spain Department of Ophthalmology Hospital Alto Guadalquivir Andújar (Jaén) Spain

We present a case of keratitis caused by Stenotrophomonas maltophilia in a therapeutic contact lens user with trichiasis and symblepharon. This keratitis was initially diagnosed as caused by Achromobacter xylosoxidans, but the strain was sent for species confirmation and the isolate was finally identified as S. maltophilia by means of 16S rDNA sequencing. The patient rapidly improved on administration of fortified ceftazidime. Physicians should be aware that the definitive identification of the pathogenic agent and prolonged antimicrobial treatment according to culture sensitivities in keratitis are mandatory as treatment success depends greatly on them.

关键词： Stenotrophomonas maltophilia Keratitis Ceftazidime

种植非法作物和武装冲突成为安第斯山脉地区生物多样性保护的限制因素

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AMBIO－人类环境杂志 2005年第3期34卷 201-207页

作者： Jon Fjelds Maria D.lvarez Juan Mario Lazcano blanca Len 盛岩哥本哈根大学动物博物馆纽约哥伦比亚大学 Centro de Biodiversidad y Genetica Universidad Mayor de San Simon Museo de Historia Natural UNMSM

古柯,在安第斯山脉地区过去曾为当地居民使用而种植,现在却为了外地市场需求而生产,而且经常种植在武装冲突地区。国际组织资助的根除非法作物运动迫使毒贩子和种植者不断重新开发新的种植区,因此与毒品种植相关的活动成为导致森林丧失... 详细信息

古柯,在安第斯山脉地区过去曾为当地居民使用而种植,现在却为了外地市场需求而生产,而且经常种植在武装冲突地区。国际组织资助的根除非法作物运动迫使毒贩子和种植者不断重新开发新的种植区,因此与毒品种植相关的活动成为导致森林丧失的一个主要原因。对于非法作物种植对生物多样性的影响人们只是笼统地有所了解,本文首次通过区域分析、以鸟类数据为代表,确定特别值得人们关注的地区。在一些地区如圣马尔塔(Santa Marta)、佩里哈山(Perija mountains)、达连(Darien)、哥伦比亚中安第斯山(CentralAndes)的一些地区,以及秘鲁境内的马拉尼翁(Maranon)河谷中部与瓦亚加(Huallaga)河谷中部之间的地区,保护所有物种这一目标会受到很大限制。为解决这些问题必须从根本上找原因:即国际毒品市场,长期存在的武装冲突,以及农村贫困人口除种植非法作物外缺乏别的收入来源。

关键词：生物多样性保护作物种植安第斯山脉种植区冲突武装市场需求国际组织中安第斯山

Comparability Assessments of Process Changes Made during Development of Anti-Idiotype Vaccine

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World Journal of Vaccines 2014年第1期4卷 24-32页

作者： Kathya de la Luz-Hernández Yamilé Rabasa Raquel Montesinos Dasha Fuentes Julio Felipe Santo Tomás Orlando Morales Yadira Aguilar blanca Pacheco Adolfo Castillo Ana Maria Vazquez Center of Genetic Engineering and Biotechnology Havana Cuba National Center for Laboratory Animal Breeding Havana Cuba Process Development Direction INIM Center of Molecular Immunology Havana Cuba

Racotumomab monoclonal antibody is a murine anti-idiotypic antibody. This monoclonal antibody mimics N-glycolyl-GM3 gangliosides has been tested in several clinical trials Phase I/II for breast, melanoma and non-small cell lung cancer patients as an anti-idiotypic cancer vaccine. The early production process was performed in vivo from mice ascites fluid. This process was transferred to bioreactor-based method at pilot scale followed to the scale-up of the fermentation. In this work we present a comprehensive molecular characterization of racotumomab MAb produced by the two different production scales in order to determine the impact of the manufacturing process in vaccine performance. We observed differences in glycosylation pattern and charge heterogeneity between racotumomab produced in both scales. Interestingly, these modifications had no significant impact on biological activity elicited in chickens. So, changes in primary structure like glycosylation, charge heterogeneity and oxidation did not affect biological activity of the vaccine.

关键词： Comparability Studies Monoclonal Antibody Cancer Vaccine Mass Spectrometry

Anthropometric Analysis of the Upper Eyelid and Eyebrow in Patients Attended by the Plastic and reconstructive Surgery Service of the Hospital Central Sur De Petróleos Mexicanos

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Journal of Biosciences and Medicines 2022年第8期10卷 47-56页

作者： Alejandro Costa-Dulché Jaime García-Espinoza Daniel De-Luna-Gallardo Cuahutemoc Márquez-Espriella Rodrigo Dávila-Díaz blanca Arámbula-Sánchez Marco Cuervo-Vergara David Flores-Soto Hospital Central Sur de Alta Especialidad de Petróleos Mexicanos Mexico City Mexico

Upper eyelid blepharoplasty is one of the most frequently performed surgeries by plastic surgeons worldwide. A prospective observational study was conducted to describe the anthropometric measurements of the upper eyelid and eyebrow in a Mexican population. The researcher measured the anthropometry of the eyelids and eyebrows to establish the diagnosis and surgical plan according to the checklist created and applied by the plastic and reconstructive surgery service. In anatomical position with a caliper instrument, the marginal reflex distance (MRD1), the tarsal thickness, the length of the vertical and horizontal slit, and finally the distance at which the external canthus is located with respect to the internal canthus were measured in millimeters. For the anthropometry of the eyebrows, the distance from the eyebrows to the hairline, the position in millimeters of the orbital rim with respect to the eyebrow, and the angle of its apex were measured. A sample of 210 patients was obtained, of which 65 were male and 145 females, divided by age groups. It was observed that in the three age groups, females presented a vertical palpebral fissure with a higher mean than males. Similar is the case of tarsal thickness, in which the difference was significantly greater in the female of the three age groups. On the other hand, it was observed that the horizontal slit in males was higher than that of females, while the MDR1 was similar in both sexes. Finally, it was identified that in the group of 41 to 50 years old significant decreases are observed both in the vertical cleft, tarsal thickness and the position of the external canthus compared to the group of 20 to 30 years old, this is due to the aging process. When evaluating the anthropometrics of the eyebrows, a greater distance from the trichion to the eyebrow was observed in males than in females, in contrast to the position of the eyebrow with respect to the supraorbital rim and its apex, in which it was shown that in f

关键词： Eyelid Upper-Eyelid Eyebrow Anthropometrics Facial-Analysis

High-throughput Proteomic Analysis in Chicken Lung with Avian Influenza Virus Infection by MASS Spectrometry

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High-throughput Proteomic Analysis in Chicken Lung with Avia...

Pathology Isolation and Identification of Canine Herpesvirus (CHV-1) in Mexico

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作者： Ying Wang Yue Shi Yaling Song Yixiang Zhang Joshua Yuan Susie Dai Vinayak Brahmakshatriya blanca Lupiani Sanjay reddy Susan J.Lamont Huaiiun Zhou Department of Poultry Science Texas A &M University College StationTX 77843-2472USA Department of Plant Pathology and Microbiology Texas A&M UniversityCollege StationTX 77843USA Department of Veterinary Pathobiology College of Veterinary MedicineTexas A&M UniversityCollege StationTX 77843-4467 USA Department of Animal Science Iowa State UniversityAmesIA 50011USA

Avian influenza virus(AIV) not only causes significant economic losses in poultry production,but also is of big concern to human *** objective of this study was to identify proteins associated with AIV infections in two genetically distinct,highly inbred chicken lines that differ in AIV resistance(Fayoumi is resistant and Leghorn is susceptible to AIV infection).Chickens were inoculated with 10 EID of H5N3 AIV or PBS at three weeks of *** lungs were collected at 4 days post *** with equal amount of cytosolic, membrane,nuclear and cytoskeletal fractions were extracted by differential detergent fractionation followed by

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Open Journal of Pathology 2016年第3期6卷 111-121页

作者： E. Guillermo Valdivia Lara blanca Lilia Barrón Romero Laura Cobos Marín Jessica Ileana Ángeles Solis Sofía González Gallardo Cesar Cuenca Verde Juan Antonio Montaraz Crespo Guillermo Valdivia Anda Facultad de Estudios superiores Cuautitlán Universidad Nacional Autónoma de México Estado de México México Escuela Nacional de Ciencias Biológicas Instituto Politécnico Nacional Ciudad de México México Universidad Nacional Autónoma de México Ciudad Universitaria Ciudad de México México Facultad de Estudios superiores Cuautitlán Universidad Nacional Autónoma de México Estado de México México

This work presents the pathology description, isolation and identification of canine herpesvirus (CHV-1) in Mexico, a virus that causes a generalized hemorrhagic infection in puppies from the canidae family. Methods: Isolates were obtained from puppies that died within the first four weeks of life and had lesions consistent with canine herpesvirus. results: The main gross lesions were petechial and ecchymotic hemorrhages in kidneys, liver and lungs;proliferative interstitial nephritis;multifocal necrosis in liver and kidneys;and encephalitis with intranuclear inclusion bodies. Herpesvirus was confirmed through direct immunofluorescence, electron microscopy and polymerase chain reaction for DNA polymerase and glycoprotein B genes. Discussion: Eight strains were isolated and identified as canine herpesvirus corresponding to three of the working cases with gross and microscopic lesions very similar to those described in the literature;then, isolates were confirmed by PCR gene amplification, positive reactions on immunofluorescence and observations from electron microscopy. This work represents the first report of this disease, including gross and histological lesions, and confirmation by isolation and identification of the canine herpesvirus in Mexico.

关键词： Pathology of Puppies Canine Herpesvirus PCR Electron Microscopy

Diversity and host assemblage of avian haemosporidians in different terrestrial ecoregions of Peru

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Current Zoology 2022年第1期68卷 27-40页

作者： Luz GARCIA-LONGORIA Jaime MURIEL Sergio MAGALLANES Zaira Hellen VILLA-GALARCE Leonila RICOPA Wilson Giancarlo INGA-DIAZ Esteban FONG Daniel VECCO Cesar GUERRA-SALDANA Teresa SALAS-reNGIFO Wendy FLOreS-SAAVEDRA Kathya ESPINOZA Carlos MENDOZA blanca SALDANA Manuel GONZALEZ-BLAZQUEZ Henry GONZALES-PINEDO Charlene LUJAN-VEGA Carlos Alberto Del AGUILA Yessica VILCA-HERreRA Carlos Alberto PINEDA Carmen reATEGUI Jorge Manuel CARDENAS-CALLIRGOS JoseAlberto IANNACONE Jorge Luis MENDOZA Ravinder NMSEHGAL Alfonso MARZAL Departamento de Anatomia Biologia Celular y ZoologiaUniversidad de ExtremaduraBadajoz E-506071Spain Molecular Ecology and Evolution Lab Department of BiologyLund UniversitySolvegatan 37SE-22362 LundSweden Instituto Pirenaico de Ecologıa-IPE(CSIC) Avda.Nuestra Senora de la Victoria 16Jaca 22700Spain DIreSA Direccion Regional de SaludLoreto 16001Peru Departamento Academico de Microbiologia y Parasitologia.Facultad de Ciencias Biologicas Universidad Nacional de la Amazonia PeruanaIquitos 16001Peru Facultad de Ciencias Agropecuarias Universidad Alas PeruanasLima 15823Peru EverGreen Institute-San Rafael Distrito de IndianaLoreto 16200Peru Observatorio de Aves Loreto(LBO) Distrito de San JuanLoreto 16008Peru Centro Urku de Estudios Amazonicos Tarapoto 22200Peru Servicio Nacional de Areas Naturales Protegidas por el Estado 15036Peru Sanidad Animal-Facultad de Zootecnia Universidad Nacional Agraria La MolinaLima 15012Peru Laboratorio de Microbiologia Facultad de Medicina Veterinaria y ZootecniaUniversidad CientUfica del SurLima 15067Peru Laboratorio de Analisis Clinico Moraleslab SAC MoralesSan Martin 22201Peru Asociacion Ecosistemas Andinos(ECOAN) Cuzco 08002Peru Pharmacology and Toxicology Graduate Group University of CaliforniaDavisDA 95616USA Global Health Initiative Wabash CollegeCrawfordsvilleIN 47933USA Facultad de Ciencias Biologicas Universidad Nacional Mayor de San MarcosLima 15081Peru Facultad de Medicina Veterinaria Universidad Nacional Hermilio ValdizanHuanuco10160Peru Asociacion Peruana de Helmintologia e Invertebrados Afines Lima 15054Peru Laboratorio de Ecologia y Biodiversidad Animal Universidad Nacional Federico VillarrealEl AgustinoLima 15007Peru Laboratorio de Invertebrados Universidad Ricardo Palma-Santiago de SurcoLima 15537Peru Department of Biology San Francisco State UniversitySan FranciscoCA 94132USA

Characterizing the diversity and structure of host–parasite communities is crucial to understandingtheir eco-evolutionary *** and related haemosporidian parasites are responsible forfitness loss and mortality in bird species ***,despite exhibiting the greatest ornithologicalbiodiversity,avian haemosporidians from Neotropical regions are quite ***,we analyze the genetic diversity of bird haemosporidian parasites(Plasmodium andHaemoproteus)in 1,336 individuals belonging to 206 bird species to explore for differences in diversityof parasite lineages and bird species across 5 well-differentiated Peruvian *** 70 different haemosporidian lineages infecting 74 bird *** showed that 25 out ofthe 70 haplotypes had not been previously ***,we also identified 81 new host–parasite interactions representing new host records for these haemosporidian *** outcomesrevealed that the effective diversity(as well as the richness,abundance,and Shannon–Weaver index)for both birds and parasite lineages was higher in Amazon basin ***,we also showed that ecoregions with greater diversity of bird species also had highparasite richness,hence suggesting that host community is crucial in explaining parasite *** parasites were found in ecoregions with lower bird diversity,implying that the abundanceand richness of hosts may shape the exploitation strategy followed by haemosporidian *** outcomes reveal that Neotropical region is a major reservoir of unidentified *** studies analyzing host distribution and specificity of these parasites inthe tropics will provide important knowledge about phylogenetic relationships,phylogeography,and patterns of evolution and distribution of haemosporidian parasites.

关键词： Amazonia avian malaria generalist parasite habitat specificity Haemoproteus Plasmodium specialist parasite

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炎症性肠病患者自身抗体检测及其临床意义

吉林医学院学报 1999年第2期19卷 28-29页

作者：李永哲 stcker W 崔京涛 blanca T 曲长翠中国医学科学院中国协和医科大学协和医院检验科北京 100730 德国EUROIMMUN实验免疫制品有限公司吉林市中心医院

目的；检测炎症性肠病患者血清听性粒细胞胞浆抗体，抗肠杯状细胞抗体，抗胰外分泌腺抗体三种自身抗体，并探讨三种自身抗体与ＩＢＤ中溃疡性结肠炎和克隆病的关系及临床诊断应用价值。方法：应用间接免疫荧光法。结果：ＡＮＣＮ在Ｉ... 详细信息

目的；检测炎症性肠病患者血清听性粒细胞胞浆抗体，抗肠杯状细胞抗体，抗胰外分泌腺抗体三种自身抗体，并探讨三种自身抗体与ＩＢＤ中溃疡性结肠炎和克隆病的关系及临床诊断应用价值。方法：应用间接免疫荧光法。结果：ＡＮＣＮ在ＩＢＤ患者中，常见于ＵＣ，同ＣＤ比较有显著性差异；抗肠杯状细胞状抗体，抗胰外分泌腺抗体多见于ＣＤ。结论：抗中性粒细胞胞浆抗体，抗肠杯状细胞抗体，抗胰外分泌腺抗体三种自身抗体检测。

关键词：痰症性肠病 ANCE 自身抗体检测