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A modular microfluidic platform to study how fluid shear stress alters estrogen receptor phenotype in ER+breast cancer cells

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Microsystems & Nanoengineering 2024年第1期10卷 287-299页

作者： Braulio Andrés Ortega Quesada Jonathan cuccia Rachael coates blake Nassar Ethan Littlefield Elizabeth c.Martin Adam T.Melvin cain Department of chemical Engineering Louisiana State UniversityBaton RougeLA 70803USA Department of chemical and Biological Engineering Clemson UniversityClemsonSC 29634USA Biological and Agricultural Engineering Louisiana State UniversityBaton RougeLA 70803USA Department Medicine Section Hematology and Medical OncologyTulane UniversityNew OrleansLA 70118USA

Metastatic breast cancer leads to poor prognoses and worse outcomes in patients due to its invasive behavior and poor response to *** is still unclear what biophysical and biochemical factors drive this more aggressive phenotype in metastatic cancer;however recent studies have suggested that exposure to fluid shear stress in the vasculature could cause *** this study a modular microfluidic platform capable of mimicking the magnitude of fluid shear stress(FSS)found in human vasculature was designed and *** device provides a platform to evaluate the effects of FSS on McF-7 cell line,an estrogen receptor positive(ER+)breast cancer cell line,during circulation in the *** of the effects of FSS on McF-7 cells was carried out utilizing two approaches:single cell analysis and bulk *** single cell analysis,cells were trapped in a microarray after exiting the serpentine channel and followed by immunostaining on the device(on-chip).Bulk analysis was performed after cells were collected in a microtube at the outlet of the microfluidic serpentine channel for western blotting(off-chip).It was found that cells exposed to an FSS magnitude of 10 dyn/cm^(2) with a residence time of 60 s enhanced expression of the proliferation marker Ki67 in the McF-7 cell line at a single cell *** understand possible mechanisms for enhanced Ki67 expression,on-chip and off-chip analyses were performed for pro-growth and survival pathways ERK,AKT,and JAK/*** demonstrated that after shearing the cells phosphorylation of p-AKT,p-mTOR,and p-STAT3 were ***,there was no change in p-ERK1/*** is a mediator of ER rapid signaling,analysis of phosphorylated ERαwas carried out and no significant differences between sheared and non-sheared populations were *** together these results demonstrate that FSS can increase phosphorylation of proteins associated with a more aggressive phenotype in circulating cancer *** findin

关键词： fluid breast estrogen

sRNAminer:多功能的植物小RNA分析工具

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Science Bulletin 2024年第6期69卷 784-791页

作者：李冠良陈程杰陈佩珂 blake c.Meyers 夏瑞 State Key Laboratory for conservation and Utilization of Subtropical Agro-Bioresources College of HorticultureSouth China Agricultural UniversityGuangzhou 510640China Guangdong Laboratory for Lingnan Modern Agriculture South China Agricultural UniversityGuangzhou 510640China Key Laboratory of Biology and Germplasm Enhancement of Horticultural crops in South china Ministry of Agriculture and Rural AffairsSouth China Agricultural UniversityGuangzhou 510640China center Saint LouisMO 63132USA Division of Plant Science and Technology University of Missouri-ColumbiaColumbiaMO 65211USA

Small RNAs(sRNAs),found extensively in plants,play an essential role in plant growth and *** various sRNA analysis tools have been developed for plants,the use of most of them depends on programming and command-line environments,which is a challenge for many wet-lab ***,current sRNA analysis tools mostly focus on the analysis of certain type of sRNAs and are resource-intensive,normally demanding an immense amount of time and effort to learn the use of numerous tools or scripts and assemble them into a workable pipeline to get the final ***,we present sRNAminer,a powerful stand-alone toolkit with a user-friendly interface that integrates all common functions for the analysis of three major types of plant sRNAs:microRNAs(miRNAs),phased small interfering RNAs(phasiRNAs),and heterochromatic siRNAs(hc-siRNAs).We constructed a curated or"golden"set of MIRNA and PHAS loci,which was used to assess the performance of sRNAminer in comparison to other existing *** results showed that sRNAminer outperformed these tools in multiple aspects,highlighting its *** addition,to enable an efficient evaluation of sRNA annotation results,we developed Integrative Genomics Viewer(IGV)-sRNA,a modified genome browser optimized from IGV and we incorporated it as a functional module in ***-sRNA can display a wealth of sRNA-specific features,enabling a more comprehensive understanding of sRNA *** and IGV-sRNA are both platform-independent software that can be run under all operating *** are now freely available at https://***/kli28/sRNAminer and https://***/cJchen/IG V-sRNA.

关键词： sRNAminer sRNA analysis miRNA PhasiRNA hc-siRNA IGV-sRNA

Identification of key genes responsible for cytokine-induced erythroid and myeloid differentiation and switching of hematopoietic stem cells by RAGE

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cell Research 2006年第12期16卷 923-939页

作者： Kyung L chin Delia c Tang Griffin P rodgers Molecular and clinical Hematology Branch (McHB) National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)National Institutes of HealthBethesdaMD 20892USA

We utilized a unique culture system to analyze the expression patterns of gene,protein,and cell surface antigen,and the biological process of the related genes in erythroid and myeloid differentiation and switching of hematopoieticstem cells(HScs)in response to cytokine ***-specific fragments(266)identified from five populationsof cytokine-stimulated HScs were categorized into three groups:(1)expressed specifically in a single cell population;(2)expressed in two cell populations,and(3)expressed in three or more populations. Of 145 defined cDNAs,three(2%)were novel *** two-dimensional gel electrophoresis and flow cytometry analyses showed overlappedand distinguished protein expression profiles in the cell populations *** process mapping of mRNAsexpressed in erythroid and myeloid lineages indicated that mRNAs shared by both lineages attended 'core processes,'whereas genes specifically expressed in either lineage alone were related to specific processes or cellular *** this study support the hypothesis that committed HScs(E14 or G14)cells can still be redirected to develop intomyeloid or erythroid cells when erythropoietin(EPO)is replaced with granulocyte-colony stimulating factor(G-cSF)under erythroid-cultured condition or G-cSF with EPO in myeloid-cultured environment,*** results sug-gest that genes or proteins co-expressed in erythroid and myeloid lineages may be essential for the lineage maintenanceand switching in hematopoiesis.

关键词： lineage switching hematopoietic stem cells erythroid/myeloid differentiation co-expression biological processes cytokines

clinical features and long-term outcomes of patients with colonic oligopolyposis of unknown etiology

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World Journal of Gastroenterology 2022年第48期28卷 6950-6961页

作者： Dan Feldman Linda rodgers-Fouche Stephanie Hicks Daniel c chung Division of Gastroenterology Massachusetts General HospitalBostonMassachusetts 02114United States center for cancer Risk Assessment Massachusetts General HospitalBostonMassachusetts 02114United States Division of Gastroenterology and center for cancer Risk Assessment Massachusetts General HospitalHarvard Medical SchoolBostonMassachusetts 02114United States

BAcKGROUND colonic adenomatous polyposis of unknown etiology(cPUE)is an adenomatous polyposis phenotype that resembles Familial Adenomatous Polyposis(FAP)even though no germline pathogenic variant is *** We sought to better characterize the clinical features and outcomes in a cohort of cPUE *** This is a retrospective case series of patients 18 years old or older with adenomatous oligopolyposis(between 10-100 adenomas)and negative genetic testing,identified through the Hereditary Gastrointestinal cancer Database at Massachusetts General Hospital,a tertiary academic referral center.A retrospective chart review was performed with a focus on demographics,alcohol and tobacco use,medication use,familial malignancy and polyp burden,genetic testing information,endoscopic surveillance data including the corresponding histopathology,colonic and extracolonic malignancies,mortality events,and their *** correlation and Pearson chi-square test(or Fisher's exact test)were used for continuous and categorical variables *** cPUE patients were primarily male(69%)and presented for genetic counseling at 63.7 *** 2 patients(2.9%)reported a first-degree relative with *** an average surveillance period of 12.3 years,0.5 colonoscopies per year were *** developed 2.3 new adenomas per year.4(5.7%)were diagnosed with colorectal cancer(cRc)at a mean age of 66 years,and 3 were diagnosed prior to the onset of oligopolyposis.7(10%)required colectomy due to advanced dysplasia or polyp *** respect to upper gastrointestinal manifestations,1 patient had a gastric adenoma,but there were no cases of gastric or small bowel *** surveillance,10(14%)patients died at a mean age of 72,and none were due to *** cPUE is distinct from familial adenomatous polyposis(FAP)syndrome and the use of FAP surveillance guidelines may result in unnecessarily frequent upper and lower endoscopies.

关键词： colonic polyposis of unknown etiology Multigene cancer panel colorectal cancer colectomy Surveillance Mortality

An Integrated Workflow for DNA Methylation Analysis

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Journal of Genetics and Genomics 2013年第5期40卷 249-260页

作者： Pingchuan Li Feray Demirci Gayathri Mahalingam caghan Demirci Mayumi Nakano blake c.Meyers Department of Plant & Soil Sciences Delaware Biotechnology InstituteUniversity of DelawareNewarkDE 19711USA

The analysis of cytosine methylation provides a new way to assess and describe epigenetic regulation at a whole-genome level in many *** methylation has a demonstrated role in the genome stability and protection,regulation of gene expression and many other aspects of genome function and ***-seq is a relatively unbiased method for profiling the DNA methylation,with a resolution capable of measuring methylation at individual *** we describe,as an example,a workflow to handle DNA methylation analysis,from BS-seq library preparation to the data *** describe some applications for the analysis and interpretation of these *** laboratory provides public access to plant DNA methylation data via visualization tools available at our ＂Next-Gen Sequence＂ websites（http：//***）,along with small RNA,RNA-seq and other data types.

关键词： DNA methylation BS-seq Epigenetics

Geomorphic predictors of riparian vegetation in small mountain watersheds

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Journal of Plant Ecology 2015年第6期8卷 593-604页

作者： blake M.Engelhardt Jeanne c.chambers Peter J.Weisberg Department of Natural Resources and Environmental Science University of NevadaMS 1861664 Virginia StreetRenoNV 89557USA USDA Forest Service Rocky Mountain Research Station920 Valley RoadRenoNV 89512USA

Aims Hydrogeomorphic processes operating at watershed,process zone and site scales influence the distribution of riparian ***,most studies examining the relationships between hydrogeomorphic processes and riparian vegetation are conducted at site *** quantified the relative importance of watershed,process zone and site geomorphic characteristics for predicting riparian plant community types and plant species abundances in four small mountain watersheds in central Nevada,*** We mapped riparian vegetation types and identified process zones(based on dominant geomorphic process and valley fill material)within the *** sampled sites in each combination of veg-etation type and process zone(n=184 sites)and collected data on watershed scale factors,valley and stream geomorphic characteristics and on plant cover of each geomorphic *** community types were defined by cluster and indicator species analyses of plant cover data,and related to geomorphic variables using ordination analysis(nonmetric multidimensional scaling).linear mixed effects models were used to predict abundances of indicator *** Findings Variables describing position in the watershed(elevation,contrib-uting area)that are related to gradients of temperature,moisture and stream discharge were of primary importance in predicting plant community *** describing local geomorphic setting(valley width,stream gradient,channel sediments,geomor-phic surface height)were of secondary importance,but accurately described the geomorphic setting of indicator *** process zone classification did not include position in the watershed or channel characteristics and only predicted plant community types with unique geomorphic *** small mountain watersheds,predicting riparian vegetation distribution requires explicit consid-eration of scale and geomorphic context within and among water-sheds in addition to site variables.

关键词： great basin multiscale plant community analysis process zone riparian

characteristics and Inpatient Outcomes of Primary Biliary cholangitis and Autoimmune Hepatitis Overlap Syndrome

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Journal of clinical and Translational Hepatology 2021年第3期9卷 392-398页

作者： Yi Jiang Bing-Hong Xu Brandon rodgers Nikolaos Pyrsopoulos Department of Medicine Rutgers New Jersey Medical SchoolNewarkNew JerseyUSA Liver center&center for Asian Health RWJBH-Saint Barnabas Medical CenterFlorham ParkNew JerseyUSA Division of Gastroenterology and Hepatology Rutgers New Jersey Medical SchoolNewarkNew JerseyUSA

Background and Aims:Primary biliary cholangitis(PBc)and autoimmune hepatitis(AIH)are hepatobiliary diseases of presumed immune-mediated origin that have been shown to *** aim of this retrospective trial was to use national data to examine the characteristics and outcomes of patients hospitalized with overlapping PBc and AIH(PBc/AIH).Methods:The National Inpatient Sample was used to identify hospitalized adult patients with PBc,AIH,and PBc/AIH from 2010 to 2014 by International classification of Diseases-Ninth Edition Revision codes;patients with hepatitis B virus and hepatitis c virus infection were *** outcomes measures were in-hospital outcomes that included mortality,respiratory failure,septic shock,length of stay,and total hospital *** outcomes were the clinical characteristics of PBc/AIH,including the comorbid extrahepatic autoimmune disease pattern and complications of ***:A total of 3,478 patients with PBc/AIH were included in the ***/AIH was associated with higher rates of Sjögren’s syndrome(pcrohn’s disease(pcirrhosis-related complications when compared to PBc or AIH *** were similar rates of mortality between the PBc/AIH,PBc,and AIH *** PBc/AIH group had higher rates of septic shock when compared to the PBc group(pc/AIH is associated with a lower rate of crohn’s disease,a higher rate of Sjögren’s syndrome,higher rates of cirrhosis-related complications,and significantly increased risk of septic shock compared to PBc and AIH individually.

关键词： Primary biliary cholangitis Autoimmune hepatitis Extrahepatic autoimmune diseases cirrhosis-related complications Septic shock Hospital burden

Prevalence of vitreous floaters in a community sample of smartphone users

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International Journal of Ophthalmology(English edition) 2013年第3期6卷 402-405页

作者： blake F. Webb Jadon R. Webb Mary c. Schroeder carol S. North Texas Tech University Health Sciences center Texas Tech University child Study center Yale University Department of Pharmacy Practice and Science University of Iowa Department of Psychiatry UT Southwestern

AIM: To estimate the prevalence and risk factors for vitreous floaters in the general population. ·METHODS: An electronic survey was administered through a smartphone app asking various demographic and health questions, including whether users experience floaters in their field of vision. Multivariate logistic regression analysis was used to determine risk factors. ·RESULTS: A total of 603 individuals completed the survey, with 76% reporting that they see floaters, and 33% reporting that floaters caused noticeable impairment in vision. Myopes were 3.5 times more likely (P =0.0004), and hyperopes 4.4 times more likely (P =0.0069) to report moderate to severe floaters compared to those with normal vision. Floater prevalence was not significantly affected by respondent age, race, gender, and eye color. ·cONcLUSION: Vitreous floaters were found to be a very common phenomenon in this non -clinical general population sample, and more likely to be impairing in myopes and hyperopes.

关键词： vitreous floaters myopia survey floaters age

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美国文化人类学的当代理论趋势

中南民族大学学报（人文社会科学版） 2008年第4期28卷 5-18页

作者： c.Fred blake 冉凡美国夏威夷大学人类学系

本文试图总结美国文化人类学的当代理论和方法论。这里所讨论的这些美国人类学的当代观念是基于它们的历史脉络、继承、重现、转变和对立。从20世纪60年代起,在诸如主观性、权力和民族志表述等问题上,人类学理论和方法论发生了许多非常... 详细信息

本文试图总结美国文化人类学的当代理论和方法论。这里所讨论的这些美国人类学的当代观念是基于它们的历史脉络、继承、重现、转变和对立。从20世纪60年代起,在诸如主观性、权力和民族志表述等问题上,人类学理论和方法论发生了许多非常显著的转变和分歧。这种状况起因于人类文化学研究的文学化途径,这种途径是经由格尔茨(Geertz)以及其他学术运动,如英国的文化研究(cultural Studies),特别是福柯(Foucault)的后现代主义而发展起来的。由此,人类学由来已久的喧嚣的科学和人文之间的对峙已经演绎出独立的认识论阵营,在某些情况下导致了一些大学中人类学系的分离。同时,这种分裂也激发或伴随着一种对当代全球化形势下文化人类学研究对象的定位的承认,比如本土性(indigenous)、下层(subalterns)研究,确认了这些研究对象在研究项目中具有合法的权利和合作的声音。这种转向使人们愈加关注应用人类学,同时也伴随着许多新的问题,例如"本土知识"的观念。另一个分裂是一些特定的专门研究领域的学科建制,如医学人类学、生态人类学和教育人类学等,而这些领域的每一个又都存在着其内部分歧和区别。这些正在发生的变化引起了对于博厄斯学派所传承下来的诸如文化、文化相对主义等观念的效用和学术规范的质疑。过去的30年是一个骚动激荡、分崩离析的时期,人类学研究在这个时期卷入了全球资本主义的新的文化逻辑的转换之中。

关键词：美国文化人类学当代理论方法论