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A dual-function selection system enables positive selection of multigene CRISPR mutants and negative selection of Cas9-free progeny in Arabidopsis

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aBIOTECH 2024年第2期5卷 140-150页

作者： feng-Zhu Wang Ying Bao Zhenxiang Li Xiangyu Xiong Jian-feng Li State Key Laboratory of Biocontrol Guangdong Provincial Key Laboratory of Plant ResourcesSchool of Life SciencesSun Yat-sen UniversityGuangzhou 510275China

The CRISPR/Cas9 technology revolutionizes targeted gene knockout in diverse organisms including ***,screening edited alleles,particularly those with multiplex editing,from herbicide-or antibiotic-resistant transgenic plants and segregating out the Cas9 transgene represent two laborious *** solutions to facilitate these processes rely on different selection ***,by taking advantage of the opposite functions of a D-amino acid oxidase(DAO)in detoxifying D-serine and in metabolizing non-toxic D-valine to a cytotoxic product,we develop a DAO-based selection system that simultaneously enables the enrichment of multigene edited alleles and elimination of Cas9-containing progeny in Arabidopsis *** five DAOs tested in Escherichia coli,the one encoded by Trigonopsis variabilis(TvDAO)could confer slightly stronger D-serine resistance than other *** expression of TvDAO in Arabidopsis allowed a clear distinction between transgenic and nontransgenic plants in both D-serine-conditioned positive selection and D-valine-conditioned negative *** a proof of concept,we combined CRISPR-induced single-strand annealing repair of a dead TvDAO with D-serine-based positive selection to help identify transgenic plants with multiplex editing,where D-serine-resistant plants exhibited considerably higher co-editing frequencies at three endogenous target genes than those selected by ***,D-valine-based negative selection successfully removed Cas9 and TvDAO transgenes from the survival offspring carrying inherited ***,this work provides a novel strategy to ease CRISPR mutant identification and Cas9 transgene elimination using a single selection marker,which promises more efficient and simplified multiplex CRISPR editing in plants.

关键词： CRISPR Genome editing D-amino acid oxidase Cas9-free Selection marker

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Honokiol Prevents Intestinal Barrier Dysfunction in Mice with Severe Acute Pancreatitis and Inhibits JAK/STAT1 Pathway and Acetylation of HMGB1

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Chinese Journal of Integrative Medicine 2024年第6期30卷 534-542页

作者： LI Jie CHEN Ya-feng GAO Lei LI Yi-jie feng Dian-xu Department of General Surgery Putuo HospitalShanghai University of Traditional Chinese MedicineShanghai200062China

Objective To investigate the effect of honokiol(HON)and the role of high-mobility group protein B1(HMGB1)on the pathogenesis of severe acute pancreatitis(SAP).Methods Thirty mice were numbered according to weight,and randomly divided into 5 groups using a random number table,including control,SAP,SAP and normal saline(SAP+NS),SAP and ethyl pyruvate(SAP+EP),or SAP+HON groups,6 mice in each *** of pancreas,intestine,and blood were collected 12 h after SAP model induction for examination of pathologic changes,immune function alterations by enzyme linked immunosorbent assay(ELISA),and Western *** vitro experiments,macrophages were divided into 5 groups,the control,lipopolysaccharide(LPS),LPS+DMSO(DMSO),LPS+anti-HMGB1 monoclonal antibody(mAb),and LPS+HON *** tight connection level was determined by transmission electron microscopy and fluorescein *** location and acetylation of HMGB1 were measured by Western ***,pyridone 6 and silencing signal transducer and activator of the transcription 1(siSTAT1)combined with honokiol were added to determine whether the Janus kinase(JAK)/STAT1 participated in the regulation of honokiol on *** protein expression levels of HMGB1,JAK,and STAT1 were detected using Western *** Mice with SAP had inflammatory injury in the pancreas,bleeding of intestinal tissues,and cells with disrupted *** in the SAP+HON group had significantly fewer pathological *** with SAP also had significant increases in the serum levels of amylase,lipase,HMGB1,tumor necrosis factor-α,interleukin-6,diamine oxidase,endotoxin-1,and *** in the SAP+HON group did not show these abnormalities(P<0.01).Studies of Caco-2 cells indicated that LPS increased the levels of occludin and claudin-1 as well as tight junction permeability,decreased the levels of junctional adhesion molecule C,and elevated intercellular permeability(P<0.01).HON treatment blocked these *** of

关键词： severe acute pancreatitis intestinal barrier dysfunction honokiol high-mobility group protein B1 Januskinase signal transducerand activatorof transcription1

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Enhanced High-Temperature Energy Storage Performance of All-Organic Composite Dielectric via Constructing Fiber-Re in forced Structure

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Energy & Environmental Materials 2024年第2期7卷 299-307页

作者： Mengjia feng Yu feng Changhai Zhang Tiandong Zhang Xu Tong Qiang Gao Qingguo Chen Qingguo Chi Key Laboratory of Engineering Dielectrics and Its Application Ministry of EducationHarbin University of Science and TechnologyHarbin 150080China School of Electrical and Electronic Engineering Harbin University of Science and TechnologyHarbin 150080China

Optimizing the high-temperature energy storage characteristics of energy storage dielectrics is of great significance for the development of pulsed power devices and power control *** a polymer with a higher glass transition temperature(T_(g))as the matrix is one of the effective ways to increase the upper limit of the polymer operating ***,current high-T_(g)polymers have limitations,and it is difficult to meet the demand for high-temperature energy storage dielectrics with only one *** example,polyetherimide has high-energy storage efficiency,but low breakdown strength at high *** has high corona resistance,but low high-temperature energy storage *** this work,combining the advantages of two polymer,a novel high-T_(g)polymer fiber-reinforced microstructure is *** is designed as extremely fine fibers distributed in the composite dielectric,which will facilitate the reduction of high-temperature conductivity loss for *** the same time,due to the high-temperature resistance and corona resistance of polyimide,the high-temperature breakdown strength of the composite dielectric is *** the polyimide content with the best high-temperature energy storage characteristics is determined,molecular semiconductors(ITIC)are blended into the polyimide fibers to further improve the high-temperature ***,excellent high-temperature energy storage properties are *** 0.25 vol%ITIC-polyimide/polyetherimide composite exhibits high-energy density and high discharge efficiency at 150℃(2.9 J cm^(-3),90%)and 180℃(2.16 J cm^(-3),90%).This work provides a scalable design idea for high-performance all-organic high-temperature energy storage dielectrics.

关键词： all-organic energy storage density high-temperature high-temperature breakdown strength

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Experimental quantum state compression from two identical qubits to a qutrit

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Science China(Physics,Mechanics & Astronomy) 2024年第7期67卷 1-5页

作者： Qiao Xu Lin-Xiang Zhou Tian-feng feng Shang-feng Qiu Si-Wu Li Wu-Ji Zhang Hui Luo Xiao-Qi Zhou State Key Laboratory of Optoelectronic Materials and Technologies and School of Physics Sun Yat-sen UniversityGuangzhou 510000China Hefei National Laboratory Hefei 230088China

In the realm of modern information technology,data compression technology occupies a pivotal *** advancements in quantum information technology,the need to compress large-scale qubits ensembles has become urgent,aiming to reduce the demand on quantum storage ***,existing quantum state compression schemes generally face a limitation:the particles before and after compression must reside in the same dimensional *** specific scenarios,compressing qubits into particles of higher dimensions not only enhances the efficiency of quantum state compression but also further reduces the usage of quantum storage *** we experimentally demonstrated a quantum state compression between particles of different dimensions,successfully compressing two qubits into a single *** average fidelity of the resulting qutrit with the ideal quantum state is *** study may have potential applications in future quantum information,such as increasing quantum communication bandwidth and reducing storage resource consumption in quantum computing.

关键词： quantum state compression quantum information technology optics

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Preface to the Special Issue in Celebration of Professor Shouwen Yu’s 85th Birthday

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Acta Mechanica Solida Sinica 2024年第3期37卷 361-362页

作者： Xi-Qiao feng Qunyang Li Department of Engineering Mechanics Tsinghua UniversityBeijing100084China

Shouwen Yu,Emeritus Professor of Applied Mechanics at Tsinghua University,was born on May 4,1939 in Xianyou County,Fujian Province,*** pursued his undergraduate studies from 1955 to 1958 in the Department of Civil Str... 详细信息

关键词： Fujian Preface pursue

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电脑中频药物导入治疗毛细支气管炎的疗效观察

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中国现代医生 2010年第34期48卷 17-18页

作者：冯碧红马旭升宁波北仑区人民医院浙医一院北仑分院儿科浙江宁波315800

目的观察电脑中频药物导入治疗毛细支气管炎的疗效。方法对本科于2008年1月～2009年12月收治的182例毛细支气管炎患儿随机分成两组：治疗组92例，对照组90例，年龄40天～22个月，两组患儿从年龄、病情、病程、药物治疗的种类及剂量差异... 详细信息

目的观察电脑中频药物导入治疗毛细支气管炎的疗效。方法对本科于2008年1月～2009年12月收治的182例毛细支气管炎患儿随机分成两组：治疗组92例，对照组90例，年龄40天～22个月，两组患儿从年龄、病情、病程、药物治疗的种类及剂量差异无统计学意义。治疗组于入院后，用电脑中频药物导人治疗仪对患儿进行肺部中药导入治疗，以一次性理疗贴片2片，用以配套呼吸道中药液湿润理疗贴片，贴于患儿背部3-8肋间，每次20min，每日1次，连用5d。对照组不予药物导入治疗。结果患儿治疗后咳嗽消失时间、肺部喘鸣音、湿哕音消失时间及住院时间，治疗组较对照组时间明显缩短，治疗组总有效率为97-8％，对照组为85．6％，进行肺部理疗的患儿其中有3例患儿背部贴片接触部位出现少许红色皮疹，表面无渗出及破溃。结论电脑中频药物导入治疗毛细支气管炎是一种有效且安全简便经济的治疗方法。

关键词：毛细支气管炎儿童药物导入疗效

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Attractor for the Extensible Beam Equation with Nonlocal Weak Damping on Time–Dependent Space

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Acta Mathematica Sinica,English Series 2024年第4期40卷 1115-1126页

作者： Chun Xiang ZHAO feng Juan MENG Institute of Applied System Analysis Jiangsu UniversityZhenjiang 212013P.R.China School of Mathematics and Physics Jiangsu University of TechnologyChangzhou 213001P.R.China

In this article,we consider the long-time behavior of extensible beams with nonlocal weak damping:ε(t)u_(tt)+Δ^(2)u-m(‖▽u‖^(2))Δu+‖u_(t)‖^(p_(u_(t)))+f(u)=h,whereε(t)is a decreasing function vanishing at *** the theory of process on time-dependent spaces,we investigate the existence of the time-dependent attractor by using the Condition(C_(t))method and more detailed *** results obtained essentially improve and complete some previous works.

关键词： Time-dependent attractor extensible beams equation nonlocal weak damping

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Bayesian partial pooling to reduce uncertainty in overcoring rock stress estimation

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Journal of Rock Mechanics and Geotechnical Engineering 2024年第4期16卷 1192-1201页

作者： Yu feng Ke Gao Suzanne Lacasse Norwegian Geotechnical Institute OsloNorway Department of Earth and Space Sciences Southern University of Science and TechnologyShenzhenChina

The state of in situ stress is a crucial parameter in subsurface engineering,especially for critical projects like nuclear waste *** one of the two ISRM suggested methods,the overcoring(OC)method is widely used to estimate the full stress tensors in rocks by independent regression analysis of the data from each OC ***,such customary independent analysis of individual OC tests,known as no pooling,is liable to yield unreliable test-specific stress estimates due to various uncertainty sources involved in the OC *** address this problem,a practical and no-cost solution is considered by incorporating into OC data analysis additional information implied within adjacent OC tests,which are usually available in OC measurement ***,this paper presents a Bayesian partial pooling(hierarchical)model for combined analysis of adjacent OC *** performed five case studies using OC test data made at a nuclear waste repository research site of *** results demonstrate that partial pooling of adjacent OC tests indeed allows borrowing of information across adjacent tests,and yields improved stress tensor estimates with reduced uncertainties simultaneously for all individual tests than they are independently analysed as no pooling,particularly for those unreliable no pooling stress estimates.A further model comparison shows that the partial pooling model also gives better predictive performance,and thus confirms that the information borrowed across adjacent OC tests is relevant and effective.

关键词： Overcoring stress measurement Uncertainty reduction Partial pooling Bayesian hierarchical model Nuclear waste repository

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Audio Mixing Inversion via Embodied Self-supervised Learning

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Machine Intelligence Research 2024年第1期21卷 55-62页

作者： Haotian Zhou feng Yu Xihong Wu Department of AI Music and Music Information Technology Central Conservatory of MusicBeijing 100031China Laboratory of Music Artificial Intelligence Laboratory of Philosophy and Social SciencesMinistry of EducationBeijing 100031China School of Intelligence Science and Technology Peking UniversityBeijing 100871China

Audio mixing is a crucial part of music *** analyzing or recreating audio mixing,it is of great importance to conduct research on estimating mixing parameters used to create mixdowns from music recordings,i.e.,audio mixing ***,approaches of audio mixing inversion are rarely explored.A method of estimating mixing parameters from raw tracks and a stereo mixdown via embodied self-supervised learning is *** this work,several commonly used audio effects including gain,pan,equalization,reverb,and compression,are taken into *** method is able to learn an inference neural network that takes a stereo mixdown and the raw audio sources as input and estimate mixing parameters used to create the mixdown by iteratively sampling and *** the sampling step,the inference network predicts a set of mixing parameters,which is sampled and fed to an audio-processing framework to generate audio data for the training *** the training step,the same network used in the sampling step is optimized with the sampled data generated from the sampling *** method is able to explicitly model the mixing process in an interpretable way instead of using a black-box neural network model.A set of objective measures are used for *** experimental results show that this method has better performance than current state-of-the-art methods.

关键词： Audio mixing inversion intelligent audio mixing self-supervised learning audio signal processing deep learning

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Identification of the Hammerstein nonlinear system with noisy output measurements

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Control Theory and Technology 2024年第2期22卷 203-212页

作者： Qiming Zha feng Li Ranran Liu School of Electrical and Information Engineering Jiangsu University of TechnologyChangzhou213001JiangsuChina School of Automobile and Traffic Engineering Jiangsu University of TechnologyChangzhou213001JiangsuChina

In this research, we present a methodology to identify the Hammerstein nonlinear system with noisy output measurements. The Hammerstein system presented is comprised of neural fuzzy model (NFM) as its static nonlinear block and auto-regressive with extra input (ARX) model as its dynamic linear block, and a two-step procedure is accomplished using signal combination. In the first step, in the case of input–output of Gaussian signals, the correlation function-based least squares (CF-LS) technique is utilized to identify the linear block, solving the problem that the intermediate variable connecting nonlinear and linear blocks cannot be measured. In the second step, to improve the identification accuracy of the nonlinear block parameters, an improved particle swarm optimization technique is developed under input–output of random signals. The validity and accuracy of the presented scheme are verified by a numerical simulation and a practical nonlinear process, and the results illustrate that the proposed methodology can identify well the Hammerstein nonlinear system with noisy output measurements.

关键词： Hammerstein nonlinear system Signal combination Auto-regressive with extra input Improved particle swarm optimization

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