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Comparative Performance of Microscopy and Nested PCR for the Detection of Cryptosporidium Species in Patients Living with HIV/AIDS in Abidjan (Côte d’Ivoire)

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American Journal of Molecular Biology 2023年第1期13卷 18-31页

作者： N’Docho Assi Fiacre-Tanguy Gonédélé Bi Sery Ernest Tuo Karim bialé marina danielle datcho-gouenet ayaud N’Guessan Tiacoh Landry Koffi Attoungbré Clément Koffi David Ako Ako Aristide Bérenger Vanga Bosson Abo Henriette Touré Offianan André Department of Parasitology Mycology Institut Pasteur Abidjan C&#244 te d’Ivoire Laboratory of Genetics and Species Improvement Félix Houphou&#235 t-Boigny University Abidjan C&#244 te d’Ivoire Department of Parasitology Mycology UFR Pharmaceutical and Biological Sciences Félix Houphou&#235 t-Boigny University Abidjan C&#244 te d’Ivoire

Cryptosporidium spp. infection is one of the causes of diarrhea in people living with HIV/AIDS. The objective of this study is to compare the sensitivity of microscopy and molecular biology to determine the prevalence of Cryptosporidium spp. in Patients Living With HIV (PLWH). This is a descriptive cross-sectional study conducted in three care centers for people living with HIV/AIDS in Abidjan. It took place from November 2018 to March 2020. Sociodemographic data were obtained via a questionnaire. Stool and blood samples were collected and analyzed for microscopy and Nested PCR detection of Cryptosporidium spp. Blood samples were analyzed for CD4+ count. A total of 363 stool samples were collected from the three sites. Individuals aged 40 - 50 years (36.52%) were most likely to participate in the study. HIV Type 1 accounted for 86.22% of the study population. The samples collected consisted of 47.65% diarrheal stool. Microscopic examination of the stool yielded a prevalence of 3.86% for Cryptosporidium spp. while the prevalence was 3.96% with molecular identification. No statistically significant difference was observed between these two prevalences (χ2 = 0.26;p = 0.609). CD4+ count was the factor associated with Cryptosporidium spp. infection for both microscopy (OR = 0.887, p = 0.001) and PCR (OR = 0.896, p = 0.001). This study demonstrated that Nested PCR improves the detection of Cryptosporidium spp. in patient diagnosis.

关键词： Cryptosporidium PCR Microscopy PLHIV Côte D’Ivoire

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Playing Well on the Data FAIRground:Initiatives and Infrastructure in Research Data Management

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Data Intelligence 2019年第4期1卷 350-367页

作者： danielle Descoteaux Chiara Farinelli marina Soares e Silva Anita de Waard Elsevier Inc50 Hampshire StCambridgeMA 02139USA Elsevier B.V.Radarweg 292043NX AmsterdamThe Netherlands

Over the past five years,Elsevier has focused on implementing FAIR and best practices in data management,from data preservation through *** this paper we describe a series of efforts undertaken in this time to support proper data management *** particular,we discuss our journal data policies and their implementation,the current status and future goals for the research data management platform Mendeley Data,and clear and persistent linkages to individual data sets stored on external data repositories from corresponding published papers through partnership with *** analysis of our data policies implementation confirms significant disparities at the subject level regarding data sharing practices,with most uptake within disciplines of Physical *** directions at Elsevier include implementing better discoverability of linked data within an article and incorporating research data usage metrics.

关键词： Open data Data sharing Data citation Open research

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Understanding of Socially Vulnerable Individuals on Oral Health and Access to Dentist: A Brazilian Study

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Open Journal of Preventive Medicine 2014年第11期4卷 868-875页

作者： Cristina Berger Fadel Jean Erick Langoski marina Izabela Machado Juliana Maria Stremel danielle Bordin Joao Alfredo Madalozo Departament of Dentistry State University of Ponta GrossaPonta GrossaBrazil Paulist State University AraçatubaBrazil Departament of Geography State University of Ponta GrossaPonta GrossaBrazil

Social vulnerability experienced by collectors of recyclable materials has a strong influence on their understanding and confrontations health. This research aimed to explore the relationship between oral health and perception of subjects in a context of social disadvantage, in order to support concrete alternatives for action on (oral) health conference. This was a qualitative study carried out with representatives of an autonomous community of Brazilian collectors of recyclable materials. Data were collected through interviews and focus group treated with the technique of qualitative analysis. Respondents showed perceptions of etiology and care practices on oral health promoted beliefs and values collectively instituted in a social risk territory. Also, they associate the quality of their oral health and their difficulty of public access to the context of social vulnerability. This study suggests the adoption of protective, educational and interceptive in oral health practice in order to improve and enable the oral health status of this population.

关键词： Health Care Vulnerable Populations Oral Health

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Oncosexology in côte d’Ivoire:Attitudes and Practices of Physicians Involved in the Management of Breast and Prostate Cancer

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Psycho-Oncologie 2024年第4期18卷 359-365页

作者： KouaméKonan Yvon Kouassi Bitti Adde Odo Yenahaban Lazare Toure Ouowene Prisca marina Sougue Petiori Gningayou Laurenceépouse Kamara Touré N’Guessan Manlan Prosper Mebiala Fleur Audrey Sessegnon Marie Sandrine Koffi Akissi Marie Barbara Yvonne Nogbou Sherif Traore Asmaho Akissi danielle Andree Traore-Kouassi Moctar Toure Department of Medicine and Medical Specialties UFR Medical SciencesFélix Houphouët Boigny UniversityYamoussoukro01 BP V166 Abidjan 01Côte d’Ivoire Service De Cancerologie Du Chu De Treichville Treichville01 BP V03 Abidjan 01Côte d’Ivoire

Objective:To evaluate the attitudes and practices of oncosexology in the management of breast and prostate cancer in Côte d’***:A cross-sectional,multicenter,descriptive and analytical survey carried out over 02 months from 1 November to 31 December 2022,among doctors involved in the management of breast and prostate cancer in Côte d’***:78 physicians on 114 participated in the study,with a participation rate of 79.5%.Only one doctor discussed the sexual risks associated with cancer with all his patients and 7.7%of doctors said they never broached questions of sexuality with their *** approach to sexuality was strongly associated with occupation(p=0.002).A request for oncosexological care was initiated by the patient and the partner respectively in 3.8%and 25.6%of *** 5.1%of physicians claimed to have received training in *** 92.3%of cases,doctors would like to have training on this ***:On the questions relating to sexuality,Physicians are not addressing sexuality with their patients diagnosed with breast or prostate *** lack of training in this area appears to be the main reason for this lack of communication and onco-sexological care.

关键词： Oncosexology oncosexuality breast prostate

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